Enhancement of fibronectin synthesis and fibrillogenesis by BMP-4 in cultured rat osteoblast.

The skeletal extracellular matrix produced by osteoblasts contains the glycoprotein fibronectin (Fn), which regulates the adhesion, differentiation, and function of osteoblasts. Fn fibrillogenesis is involved in the process of bone mineralization. Bone morphogenetic proteins (BMPs) can be isolated from organic bone matrix and are able to initiate de novo cartilage and bone formation. In this study, the effect of BMP-4 on Fn fibrillogenesis in cultured rat osteoblasts was examined. BMP-4 enhanced Fn synthesis and extracellular Fn assembly in primary cultured osteoblasts. In addition, the extracellular assembly of Fn from exogenously applied soluble human Fn was also increased by BMP-4. It has been reported that alpha5beta1 integrin is related to Fn fibrillogenesis. The synthesis of both alpha5 and beta1 integrins was upregulated by BMP-4. Immunocytochemistry showed that the clustering of alpha5 and beta1 integrins was also increased by BMP-4. BMP-4 increased fibril formation of Fn and the adhesion of osteoblasts onto Fn matrix, which was inhibited by disintegrin triflavin and Gly-Arg-Gly-Asp-Ser (GRGDS) peptide. Phosphorylation of extracellular signal-regulated kinase (ERK) and focal adhesion kinase (FAK) was increased by BMP-4. Enhancement of extracellular Fn fibrillogenesis and the mRNA expression of beta1 integrin by BMP-4 were inhibited by ERK kinase (MEK) inhibitor PD98059. These results suggest that the enhancement of extracellular Fn fibrillogenesis by BMP-4 in cultured osteoblasts is related to the increase of the synthesis of Fn and clustering of alpha5 and beta1 integrins. ERK is involved in the signaling pathway of BMP-4 in regulating Fn fibrillogenesis in osteoblasts.     

槲皮素磷酸酯钾对实验性心肌梗塞的影响

iv10～20mg/kg(木解)皮素磷酸酯钾使兔急性心肌梗塞范围明显缩小,ST段明显降低,并能对抗冠脉结扎后引起的血清CPK含量增加及心律失常,这些作用与普萘洛尔相似。但iv40mg/kg后作用不明显。     

Distinguishment of A 42 Capsomere Structure of Icosohedral Virion from A 32 One by Electron Microsco

ＤｉｓｔｉｎｇｕｉｓｈｍｅｎｔｏｆＡ４２ＣａｐｓｏｍｅｒｅＳｔｒｕｃｔｕｒｅｏｆＩｃｏｓｏｈｅｄｒａｌＶｉｒｉｏｎｆｒｏｍＡ３２ＯｎｅｂｙＥｌｅｃｔｒｏｎＭｉｃｒｏｓｃｏｐｅＣｎｅｎＢｉｎｇｙｉｎｇ（陈丙莺）；ＺｈｏｕＴｏｎｇ（周）（Ｄｅｐａｒｔｍｅ...     

单向中频电加助透液防治瘢痕增生

为防治瘢痕增生，寻求有效治疗方法，我们采用单向中频电加助透液治疗瘢痕增生患者３３例，痊愈显效率为６４％，总有效率为８８％。结果表明，单向中频电加助透液能增强导入作用，是防治瘢痕增生的有效方法。     

RELATIONSHIP BETWEEN THE CLINICAL USE OF ANTIBIOTIC AND PRODUCTION OF ESBLS

Extended -spectrum β -lactamases(ESBLs)aremainlyproducedbymembersofthefamilyEnter obacteriaceaewhichcanhydrolyzeβ -lactamantibi oticsincludingthethird - generationcephalosporinandaztreonam ,theESBLs- producingbacillishowedmedian -highresistancetoceftazidimeandaztreonamparticularly[1] .NowadaystheprevalenceofESBLs -producingstrainshavebeenreportedinmanyareasaroundtheworld[2 ] .ButthereisfewinformationabouttherelationshipbetweentheuseofantibioticandtheproductionofESBLs .Weperformedastud…     

Reply

Sir, Scarpioni described a patient with acute hydrothorax complicatingCAPD who was treated with repeated autologous blood instillationinto the pleural cavity together with a switch in the mode ofdialysis     

D4S1647、D6S2414基因座在中国汉族、蒙古族、藏族的遗传多态性

目的 调查D4S1647、D6S2414基因座在中国汉族、蒙古族、藏族群体中的遗传分布规律。方法 采集308份血及唾液标本应用PCR技术，扩增产物用非变性聚丙酰胺凝胶电泳分离，银染显色分析。结果 两位点各群体基因型频率分布均符合Hardy-Weinberg平衡，每一位点等位基因频率分布在各群体间无显著差异；通过对10个汉族家系的遗传模式分析，证实了两位点等位基因传递遵循孟德尔遗传规律。结论 D4S1647、D6S2414基因座在中国汉族，蒙古族，藏族群体均具有高度遗传多态性。     

131例小儿心律失常的临床分析

分析1977～1986年因各种疾病住院伴有心律失常病例131份表明:小儿心律失常的发生、病因、预后等方面与成人相比均有其特点。本组原发疾病共计22种,首位是感染中毒性心肌炎,占50.4％。发作类型以单纯性心律失常为最多见,占42.5％。全部病例采用综合治疗,大多预后良好。