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991.
为了解肝素对脂多糖诱导人肾小球系膜细胞增殖和细胞因子分泌的影响。作者采用四甲基偶氮唑蓝比色分析法及生物活性法,分别测定了人肾小球MC增殖和细胞因子的分泌情况。结果显示;在正常培养条件下,人肾上球MC分泌一定理的白细胞介素6(IL-60和肿瘤坏死因子α(TNF-α)。脂多糖诱导MC增殖及分泌IL-6,TNF-α。高浓度的肝素(500U/ml)促进MC增殖及分泌细胞因子,低浓度的肝素(5U/ml)抑制 相似文献
992.
彩色多普勒超声评价改良迷宫手术 总被引:3,自引:0,他引:3
为了解改良迷宫手术患者心房收缩功能恢复及心功能的情况,运用彩色多普勒超声来评价二尖瓣置换术时施行改良迷宫手术患者的手术前,后心房收缩功能和心功能情况。14例风湿性二尖瓣疾病患者,狭窄11例,关闭不全3例。对照组16例。术前,术后(半年至1年)测量左房,室,右房,室内径,M型测量左室收缩功能,左房后壁运动幅度。多普勒组织成像测量左,右房室瓣环运动速度。 相似文献
993.
鸡胚脊髓背角感觉神经元诱向因子的初步分离及鉴定 总被引:7,自引:1,他引:6
为探讨脊髓背角内影响SG神经元存活和生长的因素,取500只Hamburger40期鸡胚脊髓背角组织匀浆,离心后提取上清液,用SDS-PAGE电泳初步分离脊髓背角内的蛋白质。以简易细胞钓蛋白带(PBFC)技术找寻有神经细胞贴附的蛋白带并求相对迁移率(Rf)。将有神经细胞贴附的各Rf范围凝胶剪下并将胶内各蛋白带电转移至硝酸纤维膜上,用NGF、BDNF、NT-3、GDNF兔抗血清于硝酸纤维膜上分别进行W 相似文献
994.
血管内皮生长因子165反义RNA治疗人脑胶质瘤的实验研究 总被引:8,自引:0,他引:8
目的 探讨应用血管内皮生长因子165(VEGF165)反义RNA治疗人脑胶质瘤的可行性。方法 构建和鉴定反义VEGF165真核表达载体;将其转染入人脑胶质瘤细胞SHG44,检测其转染前、后的生物学性状;比较转染前、后SHG44细胞的裸鼠皮下致瘤性;分别应用免疫印迹、免疫组织化学、微血管计数、电镜和流式细胞仪检测上述改变。结果 成功构建反义VEGF165真核表达载体并在SHG44细胞获得表达,该细胞 相似文献
995.
996.
情感性精神障碍事件相关电位变异与自杀行为史的相关性 总被引:3,自引:0,他引:3
探讨情感性精神障碍患者件相关电位变异的临床意义。方法收集39例抑郁相和22例躁狂相患者,以33例正常人为对照组,记录其事件相关电位P300和CNV。结论P300和CNV临床应用价值得到初步证实,自杀行为相关的生物学指标研究中需进一步重视。 相似文献
997.
The prevalence of NIDDM and IGT and related factors among residents in some areas of Hubei Province, China 总被引:2,自引:0,他引:2
Wang ZZ Huang XZ Tang SB Chen YM Chen LG Jin ZX Luan XJ Zhang JH 《Biomedical and environmental sciences : BES》2000,13(2):148-153
The epidemiological survey of prevalence of NIDDM (non-insulin dependent diabetes mellitus) and IGT(impaired glucose tolerance) was conducted among 9450 residents aged 25-70 in some areas of Hubei Province, China. The results show that NIDDM and IGT prevalences are 2.62% and 4.48%, respectively. There is no significant difference between male and female (P > 0.05). The NIDDM prevalence in cities is slightly higher than that in countryside, but the difference is not significant (P > 0.05). However, the IGT prevalence in city is significantly higher than that in countryside (P < 0.01). The prevalence of both NIDDM and IGT is increasing along with the age of the population. It is also significantly related to the family history of NIDDM, hypertension, and high body mass index (BMI). By using stepwise logistic regression to analyse the risk factors of NIDDM, age (OR = 1.86), BMI(OR = 2.69), family history (OR = 2.84) and hypertension (OR = 2.23) entered the model (significance level is alpha = 0.05). 相似文献
998.
Adaptive response of thymocyte apoptosis and cell cycle progression induced by low dose X-ray irradiation in mice 总被引:18,自引:1,他引:17
Gong SL Liu SC Liu JX Zhang YC Liu SZ 《Biomedical and environmental sciences : BES》2000,13(3):180-188
The dose-effect of adaptive response of thymocyte apoptosis and cell cycle progression induced by whole-body X-ray irradiation (WBI) was studied in male Kunming mice. The inductive doses (D1) were 25, 50, 75, 100 or 200 mGy 6 h before the challenging doses (D2) of 1.0, 1.5 or 2.0 Gy. The changes in the percentages of the thymocyte apoptotic bodies (TAB) and the cells in different phases of cell cycle were measured with flow cytometry. The percentages of TAB decreased, the arrests of G1 and G2 + M phases diminished, and the cells of DNA synthesis of S phase increased when the D1 + D2 groups was compared with the D2 groups. When D1 was 200 mGy, the adaptive response of thymocyte apoptosis and cell cycle progression were no longer induced by low dose radiation (LDR). In addition, the extracellular fluid from the splenocytes were cultured with Con A for 48 h in vitro 24 h after 75 mGy WBI was placed in the murine thymocyte suspension from mice irradiated with 2.0 Gy WBI and co-incubated. The thymocyte apoptosis decreased. Especially, noteworthy was that the percentages of TAB after the incubation for 72 h were significantly lower than those in 2.0 Gy irradiated thymocytes (P < 0.05). These results indicate that when the mice were irradiated with 25-100 mGy (D1, 12.5 mGy/min) 6 h before 1.0-2.0 Gy (D2, 0.287 Gy/min) exposure, an adaptive response of thymocyte apoptosis and cell cycle progression may be induced under the condition of WBI, and LDR (75 mGy) may change the microenvironment of immune cells and decrease the thymocyte apoptosis. 相似文献
999.
Changes in tissue metals after cadmium intoxication and intervention with chlorpromazine in male rats 总被引:8,自引:0,他引:8
Yang XF Wang SY Zhao RC Ao SQ Xu LC Wang XR 《Biomedical and environmental sciences : BES》2000,13(1):19-25
Cadmium(Cd),one of the most dangerous heavy metals,has a very similar ionic radius to calcium(Ca),The interference of cadmium in calcium homeostasis may play an important role in cadmium toxicity.Recent reports indicate that calmodulin(CaM) inhibitors such as trifluoperazine and chlorpromazine(CPZ) could protect rodents against cadmium toxicity,It was also reported that pretreatment of mice with zine(Zn)could reduce the adverse effects induced by cadmium.The aim of this study is to determine whether Cd changes the balance of other essential metals such as Zn and copper(Cu) in rat tissues,and whether CPZ can reverse these changes which are induced by cadmium intoxication.Adult male Sprague-Dawley(SD) rats were injected intraperitoneally(ip) with cadmium chloride(CdCl2)(0.2,0.4,0.8mg Cd/kg body wight) alone and 0.4mg Cd/kg in association with CPZ(5mg/kg) daily for a week.The control animals were injected with normal saline only.The results showed that the cadmium content in the liver,kidney,and testis increased significantly with a dose-response relationship.Cadmium treatment markedly increased the Zn and Ca content in some of the tissues,Hepatic and renal metallothionein(MT) increased significantly after cadmium intoxication,CPZ treatment,howerver,reduced cadmium content in liver,but not blood and kidney.CPZ seemed to decrease the content of MT in liver and significantly increase the amounts of MT in kidney.These data suggest that the intervention of cadmium with tissue essential metals may play a role in cadium toxicity in rats,and calmodulin inhibitors to some extent can reduce the adverse effect of cadmium by decreasing the cadmium load in tissues and reversing the unbalance of essetial metals. 相似文献
1000.
用RT-PCR技术从人胎盘组织内成功地扩增全长肝细胞生长因子(HGF)cDNA基因(2 184bp),并将其克隆至pGEM-T载体,经限制性核酸内切酶NdeⅠ,BglⅡ,HindⅢ,BamHⅠ和XhoⅠ的酶谱分析和DNA测序分析证实。再将其亚克隆至逆病毒载体pLNL-XHC,可进一步用于基因表达和基因治疗的研究。 相似文献