全文获取类型
收费全文 | 312632篇 |
免费 | 30552篇 |
国内免费 | 23842篇 |
专业分类
耳鼻咽喉 | 2559篇 |
儿科学 | 2883篇 |
妇产科学 | 3375篇 |
基础医学 | 37194篇 |
口腔科学 | 5632篇 |
临床医学 | 44176篇 |
内科学 | 42911篇 |
皮肤病学 | 2974篇 |
神经病学 | 16589篇 |
特种医学 | 11547篇 |
外国民族医学 | 216篇 |
外科学 | 30063篇 |
综合类 | 56395篇 |
现状与发展 | 90篇 |
一般理论 | 30篇 |
预防医学 | 20233篇 |
眼科学 | 9308篇 |
药学 | 34082篇 |
388篇 | |
中国医学 | 19857篇 |
肿瘤学 | 26524篇 |
出版年
2024年 | 890篇 |
2023年 | 4625篇 |
2022年 | 11283篇 |
2021年 | 16789篇 |
2020年 | 12926篇 |
2019年 | 10823篇 |
2018年 | 11342篇 |
2017年 | 10442篇 |
2016年 | 9697篇 |
2015年 | 14714篇 |
2014年 | 18142篇 |
2013年 | 15963篇 |
2012年 | 23846篇 |
2011年 | 26856篇 |
2010年 | 17121篇 |
2009年 | 13564篇 |
2008年 | 17124篇 |
2007年 | 16742篇 |
2006年 | 16540篇 |
2005年 | 16173篇 |
2004年 | 9758篇 |
2003年 | 8854篇 |
2002年 | 7306篇 |
2001年 | 6439篇 |
2000年 | 7096篇 |
1999年 | 7656篇 |
1998年 | 4956篇 |
1997年 | 4946篇 |
1996年 | 3869篇 |
1995年 | 3657篇 |
1994年 | 3015篇 |
1993年 | 2062篇 |
1992年 | 2294篇 |
1991年 | 1916篇 |
1990年 | 1636篇 |
1989年 | 1378篇 |
1988年 | 1174篇 |
1987年 | 987篇 |
1986年 | 768篇 |
1985年 | 629篇 |
1984年 | 355篇 |
1983年 | 221篇 |
1982年 | 120篇 |
1981年 | 128篇 |
1980年 | 86篇 |
1979年 | 95篇 |
1978年 | 4篇 |
1976年 | 3篇 |
1975年 | 3篇 |
1926年 | 2篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
31.
Effects of the Clearing the Lung and Dissipating Phlegm Method in the Treatment of Acute Exacerbation of Chronic Obstructive Pulmonary Disease: A Systematic Review and Meta-Analysis 下载免费PDF全文
Tao Zhang Yao-Hui Li Gai-Xia Liu Xiu-Lin Tang 《World Journal of Traditional Chinese Medicine》2019,(1)
Objective: The objective of this study is to evaluate the efficacy and safety of the clearing the lung and dissipating phlegm method in the treatment of acute exacerbation of chronic obstructive pulmonary disease(COPD) and to provide evidence for the treatment of the disease. Materials and Methods: Literature was searched from the United States National Library of Medicine(PubMed), Embase, Cochrane Library, China National Knowledge Infrastructure, Wanfang Database(Wanfang), and the Full?Text Database of Chinese Scientific and Technical Periodicals(VIP).A comprehensive collection was made of randomized controlled trials(RCTs) before June 2018, in which the treatment groups used either the clearing the lung and dissipating phlegm formulas only or combined it with routine Western medicine therapy, and the control group adopted routine Western medicine therapy only for the acute exacerbation of COPD. The Cochrane risk of bias method was used to evaluate the quality of the literature. The data were analyzed and retrieved independently by two reviewers before meta?analysis was carried out with RevMan 5.3 software to evaluate the primary outcome measures, including the total clinical effective rate, and the secondary outcome measures such as the pulmonary function(forced vital capacity [FVC], forced expiratory volume in the 1 s [FEV1], percentage of FEV1 [FEV1%], and FEV1/FVC)and blood gases(PaO_2 and PaCo_2). Results: A total of 13 RCTs involving 990 patients(496 in the treatment group and 494 in the control group)were included in this study. Meta?analysis revealed significant difference in the efficacy of the group that adopted solely the routine Western medicine method and the group that combined the Western medicine with the clearing the lung and dissipating phlegm method. Outcome measures including the pulmonary function(FVC, FEV1, FEV1%, and FEV1/FVC) and the blood gases(PaO_2 and PaCo_2) were significantly improved as compared to the control group(P 0.00001). However, adverse effects in the treatment group using combined traditional Chinese medicine were not reported due to the short observation time of the study. Conclusion: The clearing the lung and dissipating phlegm method can improve the efficacy in the treatment of acute exacerbation of COPD, the outcome measures of the pulmonary function and the blood gases,as well as the life quality of the patients. However, due to the fact that the existing studies are generally of poor quality in which randomization and its implementation were not properly carried out, more high?quality RCTs are necessary to confirm the findings of this study. 相似文献
32.
33.
Yan Gao Shi-Feng Chu Zhao Zhang Qi-Di Ai Cong-Yuan Xia Hui-Yong Huang 《Journal of Asian natural products research》2019,21(8):782-797
Inappropriate use of acetaminophen (APAP) can lead to morbidity and mortality secondary to hepatic necrosis. Ginsenoside Rg1 is a major active ingredient in processed Panax ginseng, which is proved to elicit biological effects. We hypothesized the beneficial effect of Rg1 on APAP-mediated hepatotoxicity was through Nrf2/ARE pathway. The study was conducted in cells and mice, comparing the actions of Rg1. Rg1 significantly improved cell survival rates and promoted the expression of antioxidant proteins. Meanwhile, Rg1 reduced the excessive ROS and the occurrence of cell apoptosis, which were related to Nrf2/ARE pathway. Expression of Nrf2 has a certain cell specificity.
34.
35.
36.
37.
38.
文题释义:
细胞膜片技术:是在体外接种培养高密度的细胞,使其相互融合生长至100%而形成的透明致密膜状物。该技术不需要胰酶消化即可收集细胞,因此保留了大量的胞外基质、细胞间连接以及细胞-基质连接等结构。目前细胞膜片技术已成为组织工程领域的研究热点,已被推广应用于牙周膜、角膜、心脏、软骨、食管等多种组织器官修复。
成骨细胞:主要由内外骨膜和间充质始祖细胞分化而来,在复杂的骨形成过程中发挥着主要的功能,承担着骨基质的合成、分泌和矿化。骨髓间充质干细胞具有多向分化潜能,能定向分化为成骨细胞,其成骨分化过程可受多种因素的影响,如细胞因子的调控、遗传因素和激素水平等。背景:现阶段骨形态发生蛋白2和碱性成纤维生长因子2对骨髓间充质干细胞膜片增殖、成骨分化的影响和作用机制还尚未可知,如何将生长因子与组织工程细胞膜片技术相整合,最终将其用于骨缺损修复具有重要意义。
目的:探讨单独及联合应用骨形态发生蛋白2和碱性成纤维生长因子2对骨髓间充质干细胞膜片增殖和成骨分化的影响。
方法:体外分离培养鉴定SD大鼠骨髓间充质干细胞并构建细胞膜片,选用不同质量浓度的骨形态发生蛋白2和碱性成纤维生长因子2单独及联合诱导骨髓间充质干细胞膜片,CCK-8法结合碱性磷酸酶活性检测确定2种因子促进膜片增殖和成骨分化的最佳有效质量浓度;然后对骨髓间充质干细胞膜片进行成骨诱导,通过大体及显微镜观察、Vonkossa染色、茜素红染色、RT-PCR检测相关成骨标志物来评估诱导效果。
结果与结论:单独应用骨形态发生蛋白2可增强骨髓间充质干细胞膜片的碱性磷酸酶活性,最佳质量浓度为100 μg/L(P < 0.001),单独应用碱性成纤维生长因子2能加速骨髓间充质干细胞膜片的增殖,最佳质量浓度为20 μg/L(P < 0.001),而联合应用既可以促进膜片增殖又能提高其碱性磷酸酶活性(P < 0.001);经成骨诱导后,4组膜片在形态学上无明显差异,均能诱导骨髓间充质干细胞膜片的成骨分化,其中联合组钙结节最明显(P < 0.001),可显著促进膜片晚期成骨分化并抑制其早期成骨分化,具有明显的协同促进作用(P < 0.001)。结果表明,骨形态发生蛋白2和碱性成纤维生长因子2联合应用时具有协同作用,既可以促进骨髓间充质干细胞膜片增殖,又能显著增强其成骨诱导能力。ORCID: 0000-0003-1918-579X(何惠宇)
中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程 相似文献
39.
40.