表面等离子共振技术在新药开发研究中的应用进展

表面等离子共振技术(SPR技术)在基因工程药物研究中的应用发展迅速。文章综述了近年来SPR技术在β-淀粉样蛋白(Aβ)沉积的保护蛋白质研究及抗癌新药和艾滋病病毒新抑制剂筛选中的应用进展。     

血培养病原菌的变迁及结果分析

目的调查分析本院2004-2006年血培养主要病原菌的变迁,为临床的诊治提供依据。方法分析3年来血培养的致病菌种类及临床分布。结果从4 579份血液标本中共分离出471致病菌,阳性率为10.29%。分离率居前五位的病原菌分别是大肠埃希菌130株,真菌62株,肺炎克雷伯氏菌55株,凝固酶阴性葡萄球菌51株及金黄色葡萄球菌47株。临床送检血培养以肾脏内科最多,达899份。结论了解血培养主要病原菌的变迁趋势和临床分布情况,有助于临床确立诊疗方案。     

Marchiafava-Bignami病1例报告

Marchiafava-Bignami病(MBD)是一种罕见的大脑白质脱髓鞘性疾病,以胼胝体受累为突出表现,是导致胼胝体变性、渐进性脱髓鞘和坏死的中毒性疾病,大多见于慢性酒精中毒的患者.     

Unusual morphologic features of low‐grade endometrial stromal sarcoma: A case report

BackgroundEndometrial stromal tumours are uncommon tumours of the uterus. They mainly occur in perimenopausal women. Tumours with typical clinicopathological features do not usually pose diagnostic problems. However, rare clinicopathological features can occur, and clinicians without significant experience may have difficulty diagnosing these tumours and managing these patients.MethodsHerein, we report a case of endometrial stromal sarcoma that occurred in a 25‐year‐old woman. The pathological features, immunophenotype, treatment and prognosis were discussed.ResultsThe tumour revealed morphological heterogeneity, and there were similar proliferative‐type endometrial stromal cells, an extensive amount of mature adipose tissue, and prominent rhabdomyoblastic and smooth muscle cells. Histopathological and immunohistochemical studies confirmed low‐grade endometrial stromal sarcoma with smooth muscle, adipocytic and rhabdomyoblastic differentiation (approximately 60% were differentiated tissues). The final treatment of the tumour was total abdominal hysterectomy with bilateral salpingo‐oophorectomy. There was no evidence of recurrence for 109 months postoperatively.ConclusionsWe found that low‐grade endometrial stromal tumours with extensive adipocytic and prominent rhabdomyoblastic differentiation are misdiagnosed because they are infrequent. They must be differentiated from rhabdomyosarcoma with accurate identification of adipocytes, and long‐term follow‐up is needed.     

SB203580和WP631对照射后肺成纤维细胞内Smad通路信号转导的影响

背景与目的:放射性肺纤维化以成纤维细胞增殖和细胞外基质过度沉积为特征.转化生长因子β1 (transforming growth factor β1,TGFβ1)是放射性肺纤维化发生发展的"开关"性分子并被作为治疗的靶分子,因此阻断TGFβ1的信号转导可能减轻肺纤维化.本研究检测Smad通路抑制剂SB203580和WP631对照射后人肺成纤维细胞(human lung fibroblast, HLF)中信号转导的影响并探讨其效应.方法:将HLF用25μmol/L SB203580和5nmol/L WP631预处理后,再应用3 Gy 60co γ射线照射和10μg/L TGFβ1 刺激,通过凝胶阻滞电泳(electrophoretic mobility shift assay, EMSA)、免疫印迹、免疫组织化学及细胞周期流式细胞仪检测等方法检测转录因子SP1和AP1活性的变化、Smad3,4,7和p-Smad3 及Ⅰ型胶原和Ⅰ型组织纤溶酶原激活物抑制剂 (typeⅠplasminogen activator inhibitor,PAI-1)的表达变化及细胞周期的变化.结果:经3 Gy γ射线照射及TGFβ1 刺激后,与未经预处理的HLF相比,应用SB203580或WP631预处理的HLF出现G2-M 期阻滞,S期细胞显著减少.SB203580预处理可减少HLF中P21WAF1/CIP1和p-Smad3的表达,而WP631预处理可减少HLF表达PAI-1,抑制转录因子SP1和AP1的活化.结论:SB203580和WP631通过阻断TGFβ-Smad通路的信号转导,抑制γ射线和TGFβ1 作用所导致的HLF过度增殖和P21WAF1/CIP1及PAI-1表达增多.     

The effect of apolipoprotein E gene polymorphism and Lp(a) levels on coronary artery disease with atrial fibrillation

ObjectiveTo explore the influence of apolipoprotein E (APOE) genotypes and blood lipid metabolism on coronary artery disease (CAD) with atrial fibrillation (AF).MethodsPatients with suspected CAD were consecutively enrolled and divided into groups with or without CAD and/or AF. Blood lipid levels and APOE genotypes were determined and analysed for associations with CAD and AF.ResultsA total of 2048 patients were included (400 patients without CAD or AF [controls], 126 patients without CAD but with AF, 1294 patients with CAD without AF, and 228 patients with CAD and AF). Age and lipoprotein (a) (Lp[a]) levels were significantly higher in patients with CAD and AF versus those with CAD without AF. Among patients with CAD, the E3/E3 genotype and ε3 allele frequencies were significantly lower in patients with AF than in those without AF, and the E4/E4 genotype and ε4 allele frequencies were significantly increased. Multivariate logistic regression revealed that increased Lp(a) levels and age were independent risk factors for AF in patients with CAD.ConclusionAmong patients with CAD, those with AF had increased age, ε4 frequencies and Lp(a) levels. Age and Lp(a) levels may be independent risk factors for AF in patients with CAD.     

注射用奥沙利铂对比奥沙利铂脂质体Beagle犬的解剖病理学评价

目的:比较注射用奥沙利铂和奥沙利铂脂质体对Beagle犬毒性作用的解剖病理学改变,为抗癌药物脂质体制剂的临床前安全性评价提供重要的形态学参考数据。方法:Beagle犬分为阴性对照组、空载体脂质体组、注射用奥沙利铂组、奥沙利铂脂质体低、高剂量组。静脉滴注每2周给药1次,共4次,恢复期1个月。解剖动物进行组织病理学检查。结果:与脂质体、注射用奥沙利铂和奥沙利铂脂质体相关的组织病理学变化分别为多组织器官的泡沫细胞聚集;睾丸生精细胞数量减少,附睾精子减少症和胸腺、脾脏萎缩;肝脏小肉芽肿,胸腺、脾脏萎缩。结论:通过以上同一抗癌药物不同剂型对Beagle犬的解剖病理学评价比较,为抗癌药物脂质体剂型临床前安全性评价提供了重要的形态学参考数据。     

蜂海绵Haliclonasp.化学成分的研究

对采自我罱海的蜂海绵Ｈａｌｉｃｌｏｍａｓｐ．的化学成分进行了研究。结果表明：从Ｈａｌｉｃｌｏｎａｓｐ．中分离出２个结晶性化合物１和２。应用波谱分析和元素分析鉴定１和２的结构分别为１，３－二甲基黄嘌呤和对羟基苯甲酸。这是从海绵中分离出上述化合物的首次报道。     

内生真菌SEF15927对截短侧耳素的微生物羟化研究

目的 研究植物内生真菌SEF15927对截短侧耳素的转化作用.方法 利用液体培养生长细胞对截短侧耳素进行生物转化.发酵液经乙酸乙酯萃取和硅胶柱层析分离获得一个羟化产物.运用质谱和核磁共振光谱鉴定结构.结果 转化产物为2-羟基截短侧耳素.结论 植物内生真菌SEF15927为一具有高转化率的截短侧耳素羟化菌株,     

焦谷氨酸油醇酯促进药物经皮吸收活性的研究及其合成

用谷氨酸和油醇合成焦谷氨酸油醇酯。在该化合物作用下替硝唑、咖啡因和 醋酸可的松经皮渗透分别增大了41．8、9．1和2．9倍。