Identification of a proteomic biomarker associated with invasive ST1, serotype VI Group B Streptococcus by MALDI-TOF MS

Background

Group B Streptococcus (GBS) is an important invasive pathogen in neonates, pregnant women and the elderly. Serotype VI GBS, which has been rarely reported globally, has emerged as a significant pathogen in Asia. However, traditional serologic latex agglutination (LA) methods may fail to type isolates that lack of or low expression of CPS.

STAT3 couples with 14-3-3σ to regulate BCR signaling,B-cell differentiation,and IgE production

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心室晚电位的时间－频率表示法的进一步改进

在心室晚电位的研究中，我们引入了信号的时间一频率表示法，并根据晚电位的具体特性用Ｗｉｇｎｅｒ分布法（维格纳分布）把信号表示为在时间及频率空间中的能量分布。由于Ｗｉｇｎｅｒ分布的优异性质使我们有可能更准确表示出心室晚电位的存在。但当输入信号是两个信号的线性叠加时，Ｗｉｇｎｅｒ分布的结果会出现一个交叉项，相当于引入干扰。针对此不足，作者介绍改进方案，消除部分交叉项，收到较好的效果，并给出一些仿真及应用实例。     

山莨菪碱对中性粒细胞糖皮质激素受体的影响

以~3H地塞米松(Dex)为特异性配基,以一点分析法测得大白鼠腹腔中性粒细胞(PMNs)的糖皮质激素受体(GCR)位点数为5270±216/个PMN(n=18,X±SE下同);体外实验,PMNs直接与山莨菪碱(654-23×10~(-5)M)温育,对GCR无明显影响,测得位点数为4896±360/个PMNs(n=20,P>0.4);体内实验表明,给大鼠肌肉注射654-2(10mg/kg)每日两次共二天,则GCR位点数降至2740±101/个PMNs(n=18,P<0.001)。此时还测得血浆皮质酮含量明显增高(P<0.01)。提示654-2对GCR的影响可能与增加皮质激素所致的“降调节”有关。     

采用微孔聚丙烯纤维材料的“四川联大Ⅰ型”管外流式(ELF)中空纤维膜式氧合器的研制

介绍了“四川联大Ⅰ型”（以下简称：ＳＵＵⅠ）管外流式（ＥＬＦ）中空纤维膜式氧合器的研制、设计、外形制做及离心封端等过程，重点介绍了中空纤维膜材料选择，膜肺离心封端的原理、材料、设备及方法，并对聚氨酯胶进行离心封端的具体步聚作了较详细的叙述。作者根据自身在国外与国内的研制经验，认为离心封端的关键在于：（１）中空纤维膜材料的质量及成束质量；（２）聚氨酯封端胶材料固化特性的掌握及料量计算；（３）反应温度控制；（４）气体排除；（５）转速的控制；（６）纤维束的安装；（７）脱模和切头的时间掌握等方面。作者用外购的材料和自己设计制造的离心封端机成功地制造了我国第一个管外流式（ＥＬＦ）中空纤维成人氧合器。ＳＵＵⅠ膜式氧合器的设计是作者在国外建立的交叉流式膜肺Ｏ２、ＣＯ２传递模型的具体应用。     

应用PCR技术检测恙螨体内恙虫病立克次体动态和经卵传递的研究

本文应用聚合酶链反应技术检测恙虫病立克次体实验感染的恙螨体内立克次体的动态变化，通过人工腹腔接种而感染的恙螨成虫，恙虫病立克次体至少能在其体内生存３６０天和经卵传递４代；通过未食幼虫叮咬病小鼠而感染的恙螨，经饱蚴、若蛹、若虫、成蛹和成虫阶段，恙虫病立克次体检测均呈阳性，至少能在成虫期体内生存２７０天和经卵传递１代。     

Requirements for CD4+ cells and gamma interferon in resolution of established Cryptosporidium parvum infection in mice.

The importance of CD4+ cells and gamma interferon (IFN-gamma) in the resolution of established Cryptosporidium parvum infection was investigated with a murine model of cryptosporidiosis in severe combined immunodeficient (SCID) mice. C. parvum-infected SCID mice were reconstituted with spleen cells from immunocompetent donors. The recipients were able to resolve their C. parvum infection by 17 days postreconstitution. Treatment of reconstituted SCID mice with either anti-CD4 monoclonal antibodies to deplete them of CD4+ cells or with anti-IFN-gamma to neutralize IFN-gamma activity reduced or eliminated their ability to resolve C. parvum infection whereas treatment with either anti-CD8 monoclonal antibodies or anti-asialo-GM1 antibodies had no effect. We also found C. parvum-specific antibodies in serum samples from two of four reconstituted SCID mice killed on postreconstitution day 17 but not in unreconstituted SCID mice. Moreover, anti-CD4-treated mice had no detectable specific antibodies to C. parvum, whereas all mice treated with either anti-CD8 or anti-asialo-GM1 had substantial levels of specific antibodies in their serum. Although the role of the specific antibody is not known, these findings clearly indicate that resolution of an established C. parvum infection in immunologically reconstituted SCID mice is dependent on both CD4+ cells and IFN-gamma.     

一次性注射器填充级HDPE专用料的研制

通过对母料载体树脂的筛选,无机填料的选择,添加性能优良的耐辐射助剂,制成了具有耐辐射性能、加工性能良好的母料,该母料的制备工艺简单。本文所述的填充级HDPE专用料是将HDPE与填充母料经过简单掺混制成的。经测试专用料满足国家医药标准YY 0114—93《医用输液、输血、注射器聚乙烯专用料》的要求。制品厂试验表明:该填充级的HDPE专用料加工性能良好,用该填充级HDPE专用料注射成型的注射器芯杆装配成一次性注射器满足国家专业标准ZBC 31009—87《一次性无菌注射器》的要求。该填充母料的加入可大大降低材料的成本,100份HDPE 7006A可添加67份填充母料,该填充级专用料的价格比HDPE树脂降低约1500元/吨,因此该填充级HDPE专用料的推广和使用具有良好的经济效益。     

老年学习记忆减退大鼠齿状回突触的定量研究

据老年大鼠在Ｍｏｒｒｉｓ水迷宫中的行为表现，将其分为老年学习记忆减退和学习记忆正常两部分，采用透射电镜观察、拍片，对齿状回中分子层触的数量和大小进行体视学定量分析。     

逆行半比目鱼肌带蒂肌瓣修复小腿远端软组织缺损

目的总结逆行半比目鱼肌带蒂肌瓣修复小腿远端软组织缺损的临床应用结果。方法 2006年10月~2012年10月,应用逆行半比目鱼肌带蒂肌瓣修复12例小腿远端软组织缺损,年龄16~42岁(平均35岁)。肌瓣表面行一期中厚网状游离植皮,供区直接缝合。结果所有的肌瓣全部成活,取得了满意的临床效果。随访1.5~3.5年,平均2.5年,8例伴有胫骨骨折者骨折全部愈合。结论逆行半比目鱼肌带蒂肌瓣具有血管解剖恒定、血运丰富以及操作简单等优点,适宜修复小腿远端软组织缺损。