Experimental Studyon Anti－tumor Effect of SplenocytesInduced by Anti－CD3McAb，PHA and IL－2

（沈张悦）（邵静芳）（王晓林）（朱慧芬）ＥｘｐｅｒｉｍｅｎｔａｌＳｔｕｄｙｏｎＡｎｔｉ－ｔｕｍｏｒＥｆｆｅｃｔｏｆＳｐｌｅｎｏｃｙｔｅｓＩｎｄｕｃｅｄｂｙＡｎｔｉ－ＣＤ３ＭｃＡｂ，ＰＨＡａｎｄＩＬ－２￥ＳＨＥＮＧｕａｎ－ｘｉｎ，ＷＡＮＧＸｉａｏ－ｌｉ...     

Efficacy of SIV/deltaB670 glycoprotein-enriched and glycoprotein-depleted subunit vaccines in protecting against infection and disease in rhesus monkeys

Immunization with an inactivated whole-virus vaccine is highly effective in preventing lentivirus infection. The viral protein(s) essential to the induction of protective responses, however, have not been identified. To define the role of virion components in the induction of protective immunity, we evaluated the efficacy of glycoprotein-enriched and glycoprotein-depleted simian immunodeficiency virus (SIV) subunit vaccines prepared by lentil-lectin affinity chromatography of gradient-purified virions using the immunization and challenge regimen previously found successful with an inactivated whole-virus vaccine. Infection was determined by successful recovery of virus, the induction of SIV-specific antibody responses, and infection of naive recipients by inoculation with lymph-node-derived lymphocytes from the vaccinates. Immunization with the glycoprotein-enriched preparation prevented infection in two out of four monkeys, whereas the glycoprotein-depleted vaccine failed to prevent infection in all four vaccinates tested. However, the glycoprotein-depleted vaccine appeared to moderate the progression of SIV-induced disease compared with non-immunized infected control monkeys inoculated with the same challenge dose. These data suggest that subunit vaccines containing sufficient quantities of viral glycoproteins can protect against SIV infection, whereas subunit vaccines composed predominantly of viral core proteins cannot. The development of effective vaccines against HIV infection should include studies on the optimum presentation of the viral envelope glycoproteins to produce long-term broadly protective immune responses.     

实验性急性胰腺炎肺内细胞凋亡状况及其意义的初步探讨

目的：探讨重症急性胰腺炎时肺内细胞凋亡的状况及其在肺损伤发病机制中的意义。方法：以不同浓度牛磺胆酸钠液逆行胰胆管注射造成大急急性水肿性胰腺炎（AEP)与急性坏死性胰腺炎(ANP)两种模型，测定血浆TNF-α与内毒素水平的动态变化，免疫组化检测肺内TNF-α的表达，并以TUNEL法结合激光扫描共聚焦显微镜检测肺组织切片内细胞凋亡的情况。结果：正常时大鼠肺内偶见淋巴细胞及纤维母细胞等发生凋亡，诱导AEP或ANP后凋亡细胞数量无明显变化。随着肺损伤的出现，少许浸润的炎细胞、肺泡上皮细胞及血管内皮细胞等发生了凋亡。凋亡指数(‰)在ANP组呈一过性下降，在ANP组表现为持续下降，在6h后各时点均显著低于AEP组相应值(P＜0．05)。分析表明，ANP组血中TNF-α、内毒素含量的增加与凋亡指数的变化存在负相关(P＜0.05)。结论：ANP时肺内浸润的以中性粒细胞为代表的大量炎细胞出现延迟凋亡，这种现象可能是肺损伤发生的重要前提，同时内毒素血症及TNF-α的过度合成可能是中性粒细胞延迟凋亡的部分原因。     

Duchenne型肌营养不良症

作者综述了１０年来对Ｄｕｃｈｅｎｎｅ型肌营养不良症（ＤＭＤ）的研究概况。主要包括①ＤＭＤ的临床研究。②血清生化研究表明ＣＫ、ＬＤＨ、Ｍｂ是诊断ＤＭＤ病人和携带者的敏感指标。③心脏无创性检测和肌肉超微结构研究。④部分抗肌萎缩蛋白基因ＹＡＣ物理图谱，精细限制酶图谱和缺失热区的核苷酸顺序分析，首次发现内含子中ＡＴ富集区的同源顺序与ＤＭＤ断裂有关。⑤抗肌萎缩蛋白的缺失热区疏水肽段存在与否与ＤＭＤ发病密切相关。     

Study on lymphocyte activation and proliferation induced by anti-CD3 McAb

Summary T cell activation and proliferation via CD₃-TCR complex were investigated by lymphocyte DNA synthesis in vitro. Several interfering factors were also discussed. The result indicated that lymphocyte activation and proliferation are calciumdependent. A rise of cytoplasmic free Ca²⁺ quickly following activation with CD₃ McAb is mainly due to intracellular mobilization of Ca²⁺, while lymphocyte proliferation needs both intracellular mobilization of Ca²⁺ as well as influx of extracellular Ca²⁺. It was confirmed that CTX sensitive G protein plays a role in regulating T cell proliferation by pretreatment with CTX suppressing lymphocyte³H-TdR incorporation obviously. PLC and PKC inhibitor neomycin and P. S. S could also decrease T cell proliferation.     

The Inhibition of Apoptosis of Hepatoma Cells Induced by HBx Is Mediated by Up-regulation of Survivin Expression

Abnormalityofproliferationandapoptosisinma lignanttumorsisubiquitousandinvolvedmanygenes.Studiesshowedthathepatocellularcarcinoma(HCC) ,oneofthemostcommonmalignancesinChi na ,hasmanyabnormalexpressionofgenesrelatedtoproliferationandapoptosis .Recentstudiesindicatedthatsurvivin ,whichbelongstothefamilyofapopto sisinhibitionprotein ,hadahighexpressionlevelinHCCandwasrelatedtothemechanismsofabnormalapoptosisofHCCcells[1] .PatientswithHCCinChi nashowedahighinfectiousrateofover 80 %ofhep atit…     

X-刀立体定向放射治疗颅内肿瘤的近期疗效分析

目的:分析立体定向X-刀放射治疗颅内肿瘤后的近期疗效,并进行阶段性工作总结。材料和方法:53例不同类型脑瘤患者,以CT、MR、DSA图像资料作为定位依据,用德国Brain LAB治疗计划系统设计三维优化方案,西门子MD-2型直线加速器旋转照射。结果:53例患者经X-刀治疗后,近期均取得了满意效果,诸如以卡氏评分法评价,临床症状有所改善;以CT增强扫描复查观察,肿瘤大小趋于缩小或消失。结论:立体定向放射治疗后的追踪观察表明,只要严格掌握适应症,精确定位,精确计划,精心治疗,X-刀是一种安全、有效、无痛苦的治疗脑肿瘤的方法。它可起到改善临床症状,提高患者生活质量,延长生存期限的作用。     

血清MUC1粘蛋白IRMA及其初步临床应用

建立有效的ＭＵＣ１粘蛋白免疫放射分析法（ＩＲＭＡ），并初步用于乳腺肿瘤的诊断。利用纯化的ＭＵＣ１及其多抗，建立ＭＵＣ１双抗夹心ＩＲＭＡ法，对５５例健康女性、１１例乳腺良性肿瘤、２７例乳腺癌及２８例乳腺癌术后患者进行血清ＭＵＣ１的测定。结果：血清ＭＵＣ１ＩＲＭＡ的灵敏度为０７４ｋＵ／Ｌ；健康女性、乳腺良性肿瘤、乳腺癌及乳腺癌术后患者血清ＭＵＣ１的平均含量分别为７２３±３６７、１７８０±１０５６、３００４±２３８４和３１８４±２４７９ｋＵ／Ｌ；正常值上限为１３５４ｋＵ／Ｌ，假阳性率为１８２％；乳腺肿瘤患者血清ＭＵＣ１水平较健康女性明显提高；乳腺癌患者血清ＭＵＣ１水平高于乳腺良性肿瘤患者，但两者的阳性率（７７８７％和７２７０％）差异无显著性（χ２＝０１５４，Ｐ＞００５）；乳腺癌与乳腺癌术后患者血清ＭＵＣ１水平差异无显著性（ｔ＝－０２２，Ｐ＞００５）。ＭＵＣ１血清水平与肿瘤恶性程度成正相关，对于乳腺癌的诊断具有重要参考价值。     

Hemorrhagic fever with renal syndrome virus infection in livers studied by in situ hybridization and immunohistochemistry

ＨｅｍｏｒｒｈａｇｉｃｆｅｖｅｒｗｉｔｈｒｅｎａｌｓｙｎｄｒｏｍｅｖｉｒｕｓｉｎｆｅｃｔｉｏｎｉｎｌｉｖｅｒｓｓｔｕｄｉｅｄｂｙｉｎｓｉｔｕｈｙｂｒｉｄｉｚａｔｉｏｎａｎｄｉｍｍｕｎｏｈｉｓｔｏｃｈｅｍｉｓｔｒｙＹａｎｇＳｈｏｕｊｉｎｇ（杨守京）；Ｌ...     

帕金森氏病和肌营养不良症（Erb型）中线粒体DNA的部分缺失

用ＰＣＲ技术对１２例神经肌肉性疾病患者的１６个标本进行了线粒体ＤＮＡ缺失情况的研究。发现２例帕金森氏病患者的肌肉组织以及其中１例的外周血和另１例肌营养不良（Ｅｒｂ型）患者的肌肉组织中线粒体ＤＮＡ至少有５２６ｂｐ的缺失。还对线粒体ＤＮＡ的部分缺失与疾病发生的关系进行了讨论。