分娩镇痛的临床观察

目的 探讨布比卡因硬膜外自控镇痛和罗湃卡因蛛风膜下腔－硬膜外自控镇痛用于分娩的效果及对产程、母婴的影响。方法 ９０例初产妇分为３组,布比卡因硬膜外自控镇痛组（Ａ组,ｎ＝３０）、罗哌卡因蛛网膜下腔－硬膜外自控镇痛组（Ｂ组,ｎ＝３０）和对照组（Ｃ组,ｎ＝３０,未行分娩镇痛）,观察各组镇痛效果、产程时间、分娩方式血清皮质醇及新生儿Ａｐｇａｒ评分情况。结果 Ａ,Ｂ组镇痛效果满意,以Ｂ组为佳两组 产程未血清     

Observation on therapeutic effect of cold moxibustion on rheumatoid interphalangeal arthritis

目的:观察冷灸对类风湿性指间关节炎的临床疗效.方法:将576例类风湿性指间关节炎随机分为冷灸组290例和对照组286例,治疗时间为2个月.结果:两者总有效率差异不明显,但在第2个疗程末前者完全缓解率达77.2%,后者仅23.4%,两者差异有显著意义(P＜0.005).结论:冷灸治疗类风湿性指间关节炎效果优于西药抗炎剂.     

冷灸治疗类风湿性指间关节炎疗效观察

目的:观察冷灸对类风湿性指间关节炎的临床疗效。方法:将576例类风湿性指间关节炎随机分为治疗组(冷灸组)290例和对照组(服药组)286例,治疗时间为2个月。结果:两者总有效率虽然差异不明显,但在第二疗程末前者完全缓解率达77.2％,后者仅23.4％,经统计学处理P<0.005,两者差异有非常显著性意义。结论:冷灸治疗类风湿性指间关节炎效果优于西药抗炎剂。     

抗真菌微生物天然产物的研究进展

真菌感染疾病近年在增加．能有效治疗这种疾病的药物不多．因此．开发新的抗真菌药物成为当务之急。天然产物在新药及新药先导化合物的发现中起着重要作用。本文主要综述微生物来源的天然抗真菌药物的发展，并根据化合物作用机制将其分为细胞壁成分合成抑制剂、鞘脂类合成抑制剂、蛋白质合成抑制剂和作用于新靶点的抑制剂四类。     

23例乳腺派杰氏病诊治体会

目的总结23例乳腺派杰氏(Paget’s)病患者的诊断及治疗。方法收集我院自1984～2004年23例经病理确诊的乳腺Paget’s病例，通过对病人的临床资料及治疗方法的分析，总结该病的诊治经验。结果乳腺Paget’s病以慢性乳头皮肤改变为主要特点，钼靶X线多显示导管区恶性改变。细胞学检查见到Paget’s细胞。术后7例失访，余16例随访时间最长13年，最短6月，其中3例出现术后远处转移。结论早期诊断及手术为主的综合治疗预后较佳。     

Enhancement of manumycin A-induced apoptosis by methoxyamine in myeloid leukemia cells.

Farnesyltransferase inhibitors (FTIs) are currently under investigation for leukemia treatment. We evaluated the FTI manumycin A (manumycin) in two myeloid leukemia cell lines (U937 and HL-60). Manumycin induced nitric oxide production and apoptosis of the leukemia cells. Nitric oxide or other reactive oxygen species may induce oxidative DNA damage, and the number of apurinic sites increased after manumycin treatment, which was reversed by concurrent treatment with N-acetyl-L-cysteine. Since repair of DNA damage is important to cell survival, we hypothesized that methoxyamine, an inhibitor of base-excision repair, would enhance the antineoplastic effect of manumycin. The combination of manumycin and methoxyamine resulted in enhanced apoptosis by six criteria increased annexin V binding, release of mitochondrial cytochrome c into the cytosol, activation of caspase-9, activation of caspase-3, specific cleavage of poly-adenosyl ribose polymerase, and increase in the sub-G1 cell cycle fraction. The drug combination enhanced inhibition on the soft agar clonogenic assay and on the formazan dye cell viability assay. The effects of manumycin or manumycin plus methoxyamine on apoptosis were blocked by N-acetyl-L-cysteine, and partially by nitric oxide synthase inhibitors or scavenger of peroxide. We conclude that methoxyamine enhances manumycin-induced apoptosis in myeloid leukemia cells.     

复方大青叶合剂主要药效学研究

目的:对复方大青叶合剂进行了与临床主治功能有关的主要药效学研究.方法:解热试验、抗炎试验和抗菌试验.结果:复方大青叶合剂能明显降低因大肠杆菌细菌内毒素引起的家兔体温升高;对腹腔注射金黄色葡萄球菌、流感杆菌引起的小鼠死亡有一定的保护作用;体外抗菌试验表明对金黄色葡萄球菌、流感杆菌、肺炎双球菌、链球菌(甲、乙型)均有较好的抑菌作用.能明显抑制角叉菜胶所致的大鼠足肿胀和醋酸所致的小鼠腹腔毛细血管通透性增加.结论:复方大青叶合剂具有解热、抗菌、抗炎作用.     

Conversional synthesis and cytotoxic evaluation of novel taxoid analogs

Four novel taxoid analogs were conversionally synthesized from the C₁₉-diterpenoid alkaloid deltaline, and their cytotoxic activities were evaluated against a small panel of cancer cell lines.     

A pilot study of nocturnal temporalis muscle activity in TMD diagnostic groups of women

Temporomandibular disorder (TMD) incidences are believed to be related to parafunctional behaviours like teeth clenching. This pilot study aimed to (i) develop an automated clench‐detection algorithm, and (ii) apply the algorithm to test for differences in nocturnal clenching in women with and without TMD. Subjects gave informed consent to participate. Adult women were categorised using Diagnostic Criteria for TMD according to presence/absence (+/?) of both TM joint disc placement (DD) and chronic pain (P) into two groups (+DD+P, ?DD?P) with 12 subjects each. Surface temporalis electromyography was recorded during oral tasks performed by subjects at two laboratory sessions. The data were used to characterise muscle activity per N of bite force (μV/N) for each subject, develop the clench‐detection algorithm and test its accuracy. Ambulatory surface temporalis electromyography was self‐recorded by each subject over three nights and analysed using the algorithm and bite force (N) versus muscle activity μV/N calibrations. Bonferroni‐adjusted homoscedastic t‐tests assessed for significant between‐group differences in clenching (P < 0·05). Sensitivity, specificity and accuracy of algorithm‐detected laboratory clenches were all ≥96%. During self‐recordings 95% of clenches had durations of <4 s and peak forces of <10 N in both groups. Mean clench durations were significantly longer (P = 0·042) in +DD+P (1·9 ± 0·8 s) than ?DD?P subjects (1·4 ± 0·4 s). Mean temporalis duty factors (%clench time/total recording time) were significantly larger (P = 0·041) in +DD+P (0·47 ± 0·34%) than ?DD?P (0·26 ±0·22%) subjects. Nocturnal temporalis muscle activities detected by a validated algorithm were longer per clench and recording time in +DD+P compared to ?DD?P women.     

葡萄糖转运蛋白1和波形蛋白在肺癌胸水中的应用价值

目的:采用免疫细胞化学技术测定葡萄糖转运蛋白1(GLUT1)及波形蛋白(VM)在癌细胞中的定位表达,探讨GLUT1和VM在肺癌胸水中的应用价值。方法:以肺癌患者为研究对象,以肺癌性胸水为检测基础,收集胸水沉淀细胞,制成石蜡切块,采用免疫组化技术-链霉菌抗生物素-过氧化物霉法检测30例肺癌胸水和20例良性胸水中GLUT1和VM蛋白表达,统计单个及联合应用价值。结果:30例肺癌胸水中,GLUT1表达阳性的有25例(86.7%),良性胸水中间皮细胞VM表达阳性的有18例(90.0%),两种不同性质的胸水GLUT1和VM的阳性率表达均有差异(P<0.05),GLUT1和VM在肺癌胸水中表达呈负相关(P<0.05),两者联合诊断灵敏度为90%。结论:应用免疫组化方法检测肺癌胸水中GLUT1和VM蛋白表达,可提高肺癌早期诊断的灵敏度。