一期动静脉转流与并加压破坏深静脉瓣对比的实验研究

目的:改进一期动静脉转流术的疗效。方法:10只狗双后肢,随机选择一侧后肢行一期动静脉转流,另一侧行一期动静脉转流加水压破坏深静脉瓣。术后30天通过动脉造影进行对比研究。结果:后者能更有效地加速远段深静脉瓣的破坏,使动脉血逆行更远,同时形成更为丰富的侧枝循环,而不会增加肢体肿胀和深静脉血栓的机会。结论:一期动静脉转流加水压破坏深静脉瓣可能是治疗严重肢体缺血更为有效的方法。     

用足孕期畸形学指标评价毫米波辐射对小鼠植入后胚胎生长发育的影响

本实验用频率36.11GHz、功率密度为10.0mW/cm~2的毫米波,在小鼠怀孕6～15d时进行2h/d的照射,在孕期终了用足孕期畸形学指标进行分析。结果显示,毫米波照射可导致足孕期孕鼠体重、体重增加数和胎鼠体重的明显降低,胎盘重明显减轻,足孕时胎仔身长及尾长均减短,照射未导致孕鼠脑、肝、肾、卵巢等脏器重量(右肾除外)及脏器/体重比值、活胎数、死胎数、吸收数等指标发生明显变化,亦未导致胎鼠外表发生畸形、内脏发生畸形,或骨骼畸形增多。     

父母文化程度与高师学生心理健康问题研究

目的 了解高师学生心理健康现状和父母文化程度对高师学生心理健康水平的影响。方法 对河南省部分高师学生进行问卷调查。结果 河南省大部分高师学生心理健康状况是好的和比较好的；15.13%的高师学生心理健康状况不良，教师、咨询人员和家长应高度重视，密切配合，正确地对他们进行引导和教育。结论 父母文化程度不同对高师学生心理健康水平的影响有差异。但差异不显著。     

美国国立医学图书馆Index Medicus/MEDLINE收录期刊的标准

就医学期刊而言，美国国立医学图书馆的Index Medicus／Medline／PubMed应是生物医学类期刊的最佳选择，是否被该检索系统收录对医学期刊及其内容的影响至关重要。期刊被该数据库的收录审查过程极其严格，涉及很多具体问题。     

纳米硒对断奶仔猪肝脏谷胱甘肽过氧化物酶和脱碘酶I活性的影响

研究了纳米硒和亚硒酸钠对断奶仔猪肝脏谷胱甘肽过氧化物酶和脱碘酶活性的影响。将纳米硒和亚硒酸钠2种硒源分别以0.1、0.2、0.3、0.4、0.5、1.0 mg/kg 6个硒水平添加到基础日粮中,配制成12种试验日粮,基础日粮作对照,饲养“杜长大”断奶仔猪(8.3 kg左右)。结果表明:以亚硒酸钠形式添加的硒浓度为1.0 mg/kg时,仔猪生长性能和GSH-Px活性显著低于0.2~0.4 mg/kg硒添加水平(P<0.05),纳米硒添加浓度为1.0 mg/kg,仔猪生长性能和GSH-Px活性仍然保持在高峰平台;硒源添加浓度为0.10~0.40 mg/kg时,两种硒源对GSH-Px活性的影响无显著差异(P>0.05);在0.50和1.0 mg/kg硒添加水平上,纳米硒组GSH-Px活性显著高于亚硒酸钠组(P<0.05)。基础日粮组肝脏脱碘酶活性显著低于各硒源的添加水平(P<0.05),硒源和硒添加水平对脱碘酶活性没有显著影响(P>0.05)。上述结果提示:纳米硒的Weinberg剂量-效应的最适剂量范围宽于亚硒酸钠。     

Reaction of chitosan with genipin and its fluorogenic attributes for potential microcapsule membrane characterization

This study investigates the fluorogenic characteristics of the chitosan-genipin reaction for applications in microencapsulation research. Results showed that the chitosan-genipin reaction generated a colored and fluorescent product, with optimal excitation and emission wavelengths at 369 and 470 nm, respectively. Furthermore, it was found that reaction conditions affected the fluorescence intensity of the product. Mixture at the ratio of 4:1 (chitosan: genipin by weight) fluoresced the most. It also fluoresced stronger if the reaction occurred at higher temperature, with the intensity of 10.4 x 10(5) CPS at 37 degrees C, 5.9 x 10(5) CPS at 20 degrees C, and 2.5 x 10(5) CPS at 4 degrees C. As well, the fluorescence of the mixture developed gradually over time, attaining the emission maxima of 2.9 x 10(5), 7.6 x 10(5), and 10.0 x 10(5) CPS in 1, 6, and 18 h, respectively. Chitosan-coated alginate microcapsules were prepared without prior labeling, to which subsequent genipin treatment was applied in order to examine the potential of using genipin in microcapsule characterization. Chitosan bound to the alginate beads interacted with genipin, from which the resultant fluorescent signals allowed for clear visualization of the chitosan coating under confocal laser scanning microscopy. The relative fluorescence intensity across the chitosan membrane was found to be considerably higher than the controls (175 vs. 50). The membrane thickness measured was 29.2 +/- 7.3 microm. These findings demonstrate a convenient and effective way of characterizing chitosan-based microcapsules using genipin as a fluorogenic marker, a technique that will be useful in microcapsule research and other biomedical applications.     

不同注意偏移和言语材料对耳优势影响的初步分析

对５５名健康被试在：（１）无注意偏移，（２）注意右偏，（３）注意左偏等三种注意条件下进行了下述３项言语分听测验；１．分离语测验，２。数字测验，３．有鲜明形象意义词汇测验。分析了不同注意偏移和言语材料对耳优优势的影响，认为注意右偏可加强分听言语材料时的右耳优势，左偏可减弱右耳优势，不同言语材料获得耳优势的结果不同。     

Involvement of ERK, p38 and NF-kappaB signal transduction in regulation of TLR2, TLR4 and TLR9 gene expression induced by lipopolysaccharide in mouse dendritic cells

Toll-like receptors (TLR) are sentinel receptors capable of recognizing pathogen-associated molecule patterns (PAMP) such as lipopolysaccharide (LPS) and CpG-containing oligonucleotides (CpG ODN). TLR2 and TLR4 are major receptors for Gram-positive and Gram-negative bacterial cell wall components, respectively. TLR9 is necessary for CpG signalling. LPS or CpG ODN can activate immature dendritic cells (DC) and induce DC maturation characterized by production of cytokines, up-regulation of co-stimulatory molecules, and increased ability to activate T cells. However, little is known regarding the regulation of TLR gene expression in mouse DC. In this study, we investigated the regulation of TLR2, TLR4 and TLR9 gene expression by LPS in murine immature DC. TLR2, TLR4 and TLR9 mRNA were up-regulated following LPS stimulation. The up-regulation of TLR9 expression coincided with significantly increased production of tumour necrosis factor-alpha induced by LPS plus CpG ODN. While inhibition of extracellular signal-related kinase and NF-kappaB activation suppressed the up-regulation of the expression of TLR2, TLR4 and TLR9 mRNA, inhibition of p38 kinase prevented the up-regulation of TLR2 and TLR4 mRNA expression but enhanced the up-regulation of TLR9 expression. These results demonstrated that TLR2, TLR4 and TLR9 gene expression was differently regulated by LPS in mouse immature DC. Up-regulation of TLR2, TLR4 and TLR9 expression by LPS might promote the overall responses of DC to bacteria and help to explain the synergy between LPS and other bacterial products in the induction of cytokine production.     

平板运动试验不同阶段出现的室性心律失常分析

目的：通过进行平板运动试验鉴别室性早搏的性质。方法：从所有做平板运动试验的2383例室性心律失常病例中，选出在不同阶段出现室行早搏的217例患者进行分析。结果：经冠状动脉造影证实确定为冠心病的174例，高血压性心脏病合并冠心病12例，X综合症4例，心律失常15例，植物神经功能紊乱12例。174例冠心病患者7例为左主干病变，58例为双支病变，109例为单支病变。结论：平板运动试验对室性心律失常的定性分析具有较高的临床价值。     

基于稀疏分解法的单次诱发电位提取

迭代加权稀疏分解法是按照白噪声在小波的多分辨结构中的二尺度关系来确定求最小l1模优化问题时的加权系数,并通过一个迭代过程来逐步消除强噪声的影响。通过对视觉诱发电位的单次提取的研究说明了这种方法具有良好的单次提取效果,其实验结果支持单次提取的视觉诱发电位是不相同的观点。