荆防颗粒对雌性小鼠性激素的影响

目的：探讨荆防颗粒对雌性小鼠雌激素和雄激素水平的影响。方法：将4周龄雌性昆明小鼠随机分为对照组,荆防颗粒高、中、低剂量组,分别给予去离子水和荆防颗粒溶液连续灌胃4 d,检测小鼠血清雌二醇和睾酮水平,检测小鼠血清不同时间点雌二醇水平变化;将8周龄雌性C57小鼠随机分为对照组,模型组,荆防颗粒高、中、低剂量组,将小鼠背部脱去毛发,荆防颗粒组和模型组给予二氢睾酮皮下注射,对照组给予注射用大豆油皮下注射,对照组和模型组用去离子水灌胃,荆防颗粒组用荆防颗粒溶液连续灌胃18 d,观察小鼠背部毛发生长情况,检测血清睾酮、雌二醇水平,观察皮肤和卵巢病理变化。结果：荆防颗粒可以升高正常小鼠血清雌二醇水平,降低睾酮含量（P<0.05）;与模型组比较,荆防颗粒能降低外源性雌二醇的峰谷波动幅度,减缓雌二醇的代谢;与模型组比较,荆防颗粒可以升高雄激素性斑秃模型雌性小鼠血清中的雌二醇水平,降低睾酮水平（P<0.05）,并具有一定的促进毛发生长和卵巢保护作用,与对照组睾酮浓度比较无明显降低作用。结论：荆防颗粒可以促进正常雌性小鼠的雌激素分泌,对外源性性激素引起的性激素水平异常具有调节作用,并且对雄激素性斑秃模型小鼠具有一定的促毛发生长和卵巢保护作用。     

DXA、力学测试和矿盐含量评估大鼠股骨性能的精确性比较

目的 为深入了解DXA、力学测试和矿盐含量在航天骨丢失研究的应用,本文比较了这三种方法评估大鼠股骨性能的精确性,并比较了大鼠股骨在活体、离体和冷冻三种情况下的DXA测量差别.方法 选择3只雄性SD大鼠,首先对其两侧股骨进行DXA测量,得到活体、离体和冷冻三种情况的骨矿密度(BMD),然后进行力学测试和矿盐含量测试.结果 DXA、力学测试和矿盐含量的变异系数(CV)分别为7.5%～8.5%,10.1%～17.1%和0.8%.对于DXA测量,活体股骨BMD值与离体和冷冻股骨BMD值都有极其显著差别(P＜0.001),而离体与冷冻股骨BMD值无显著性差别.结论 矿盐含量精确性好于DXA和力学测试.DXA测量活体与离体骨骼差别很大,冷冻不会影响DXA测量.     

坐骨神经和T10椎体部脊髓损伤对大鼠股骨干骨折早期骨痂生成的影响

背景:神经因素在骨折愈合过程中具有调解作用,但其具体机制不明,实验进一步观察了外周神经和中枢神经在骨折愈合过程中的作用.目的:探讨失神经(坐骨神经和T10椎体部脊髓损伤)支配对Wistar大鼠股骨干骨折早期骨痂生成的影响.方法:建立Wistar大鼠单侧股骨中段开放性横行骨折动物模型,然后分别进行同侧坐骨神经横断和或T10椎体部脊髓开放性横断,并设仅行骨折建模者为对照组.所有大鼠分别于建模后14和28 d后处死并于骨折部位取材,进行影像学测量及标本新生骨痂组织学观察.结果与结论:建模14和28 d后,骨痂影像学测量结果均显示,坐骨神经横断组骨折愈合最快,对照组其次,T10椎体部脊髓横断组最慢,3组间差异均有显著性意义(P<0.05);股骨标本骨痂组织学观察显示:对照组骨痂较成熟,坐骨神经横断组和T10椎体部脊髓横断组均生成不成熟骨痂.实验以此推断周围神经损伤加速骨折愈合,中枢神经损伤则延缓骨折愈合,失神经支配下骨痂的构成有明显缺陷,完整的神经支配是骨折愈合的必要条件.     

Mechanical properties of an interpenetrating network poly(vinyl alcohol)/alginate hydrogel with hierarchical fibrous structures

Bioinspired hierarchical fibrous structures were constructed in an interpenetrating poly(vinyl alcohol, PVA)/alginate hydrogel network to improve its mechanical properties. The interpenetrating hydrogel network with hierarchical fibrous structures was prepared by combining the confined drying method and freeze–thaw method. First, Ca²⁺ cross-linked alginate formed a nano–micro hierarchical fibrous structure via the confined drying method. Then, PVA that was uniformly distributed among the Ca²⁺–alginate chains was cross-linked by hydrogen bonding via the freeze–thaw method, further dividing the hierarchical fibers into finer fibers. The results of a tensile test demonstrated that both the tensile stress and fracture energy improved by more than double after the introduction of 2 wt% PVA, achieving a combination of high strength (∼12.9 MPa), high toughness (∼13.2 MJ m⁻³) and large strain (∼161.4%). Cyclic tensile tests showed that a hysteresis loop existed on the loading–unloading curves of the hydrogel along the fibrous directions, and a good self-recovery property emerged after resting for a period of time. The hydrogel with hierarchical fibrous structures constructed by alginate and PVA can be employed in biomedical applications in the future.

The mechanical properties both along and perpendicular to the fibrous directions were improved more than double after the construction of hierarchically arranged fibrous structures in the interpenetrating network PVA/alginate hydrogel.     

Differentiation-stimulating potency of differentiated HL60 cells after drug treatment

Differentiation therapy in the treatment of leukemia is often hampered by limitations on using certain pharmaceutical regents or on the required doses due to various reasons, such as drug-resistance and retinoic acid syndrome. To circumvent these problems, a strategy might be developed on the basis of the ability of drug-differentiated cells to stimulate differentiation in leukemia cells. Using the promyelocytic leukemia cell line HL60 as a cell model, we assessed the differentiation-stimulating potency of differentiated granulocytes and monocytes/macrophages after treatments with all-trans retinoic acid (ATRA) and 12-O-tetradecanoylphorbol-13-acetate (TPA), respectively. ATRA- and TPA-differentiated cells were able to stimulate differentiation in fresh HL60 cells, accompanied by inhibition on cell growth to various extents. The differentiated cells of the second generation, especially those originated from TPA treatment, were as potent as the drugs themselves in stimulating differentiation in fresh HL60 cells. On the basis of “differentiation induced by differentiated cells”, we explored the feasibility of ex vivo therapy.     

Differential expression and analysis of extrachromosomal circular DNAs as serum biomarkers in lung adenocarcinoma

BackgroundExtrachromosomal circular DNAs (eccDNAs) increase the number of proto‐oncogenes by enhancing oncogene expression to promote tumorigenesis. However, there are limited reports on differential eccDNA expression and analysis in lung cancer, especially in lung adenocarcinoma (LAD).MethodsThree LAD and three corresponding NT tissues samples were used for eccDNA next‐generation sequencing analysis, and an additional 20 were used for quantitative PCR (qPCR) evaluations. We further performed qPCR amplification using serum samples from LAD patients and healthy medical examiners.ResultseccDNAs from LAD samples were mainly 200–1000 bp in length. Gene annotation analysis revealed that most eccDNAs were derived from chromosomes 1 and 2. The top‐ten increased and top‐ten decreased eccDNAs in LAD tissues were CircD‐ARPC1B, CircD‐ARPC1A, CircD‐FAM49B, CircD‐SDK1, CircD‐KCNG1, CircD‐POLR2F, CircD‐SS18L1, CircD‐SLC16A3, CircD‐CSNK1D, CircD‐KCTD1, and CircD‐TMIGD2, CircD‐PDIA5, CircD‐VAV2, CircD‐GATAD2A, CircD‐CAB39L, CircD‐KHDC1, CircD‐FOXN3, CircD‐SULT2B1, CircD‐DPP9, and CircD‐CSNK1D. qPCR demonstrated that the expression of CircD‐DZRN3 was higher in LAD tissues than in normal lung tissues, whereas CircD‐LGR6 and CircD‐UMODL1 expression levels were lower in LAD than in normal lung tissues. Furthermore, the serum CircD‐PDZRN3 level increased, while CircD‐LGR6 decreased in LAD. Receiver operating characteristic (ROC) analysis showed that area under curve (AUC) of serum CircD‐PDZRN3 (0.991), CircD‐LGR6 (0.916) was higher than that of serum carcinoembryonic antigen (CEA) (0.825), CY211 (cytokeratin 19 fragment) (0.842), SCCA(squamous cell carcinoma antigen) (0.857) for the diagnosis of LAD.ConclusionsOur study first showed that several eccDNAs were aberrantly expressed in LAD, among which CircD‐PDZRN3 and CircD‐LGR6 clearly distinguished LAD patients from healthy controls, indicating their potential as biomarkers.     

山西省医疗系统科技工作者疲劳症状及其影响因素的分析

目的调查山西省医疗系统科技工作者疲劳症状严重程度,分析其影响因素及其与述情障碍、应对方式及社会支持之间的相关性。方法采用自制的一般情况调查表、疲劳量表-14(FS-14)、多伦多述情障碍量表(TAC)、特质应对方式问卷(TCSQ)及社会支持评定量表(SSRS)收集相关信息。随机调查山西省医疗系统科技工作者550例,获得有效试卷518份,合格回收率94.2%。结果1山西省医疗系统科技工作者躯体疲劳、脑力疲劳及疲劳总分与王天芳等报道的健康对照组及CFS组比较均数差异均有显著性,P0.001;2躯体疲劳、脑力疲劳及疲劳总分与述情障碍因子1、因子2、因子3及述情总分均呈正相关,除脑力疲劳与因子3外,其余P0.001;躯体疲劳、脑力疲劳及疲劳总分与消极应对方式呈负相关(P0.001);三者与积极应对方式呈正相关(P0.001～0.01);除脑力疲劳与客观支持无明显相关外,余疲劳症状各因子分及总分与社会支持各因子分及总分均呈明显负相关(P0.001～0.01);3预测躯体疲劳的因素有述情总分、工作强度及支持总分(P0.001～0.01);预测脑力疲劳的因素有述情总分、行政职务、积极应对及是否了解健康方面的知识,除是否了解健康方面的知识外,余P0.05;预测疲劳总分的影响因素有述情总分、支持总分及工作强度(P0.01)。结论影响山西省医疗系统科技工作者疲劳症状的因素是多方面的,本研究结果显示以述情障碍、工作强度、社会支持、应对方式、行政职务等为主要因素。     

缺氧缺血性脑损伤新生大鼠海马突触体素的表达及当归调控作用

目的探讨脑缺氧缺血对新生大鼠海马齿状回突触体素表达的影响及当归注射对其表达的调控作用。方法取7日龄健康SD新生大鼠33只,随机分为对照组、缺氧组和当归组各11只。缺氧组和当归组新生大鼠在无菌环境下结扎左侧颈总动脉,术后护理2 h后置于三气培养箱持续缺氧2 h,制作新生鼠缺氧缺血性脑损伤（HIBD）模型,对照组仅行假手术,不结扎左侧颈总动脉、不缺氧。术后第8 d开始,缺氧组和对照组大鼠经腹腔注射生理盐水（8ml/kg）,连续7 d;当归组用等量当归注射液（250 g/L）代替生理盐水。于生后第22 d取大鼠脑组织,常规石蜡包埋、经海马切片,行突触体素（SY）免疫组化染色,图像分析海马齿状回SY阳性细胞的积分光密度（IOD）值。结果缺氧组大鼠海马齿状回SY阳性细胞的IOD值较对照组降低,而当归组SY阳性细胞的IOD值较缺氧组增高。结论脑缺氧缺血可降低新生大鼠海马齿状回SY的表达,而当归注射液对缺氧缺血性脑损伤新生大鼠可能具有保护作用。     

Expression of FAK-related non-kinase (FRNK) coincides with morphological change in the early stage of cell adhesion

Focal adhesion kinase (FAK), a protein tyrosine kinase, has recently been suggested to play a role in signal transduction through integrins. In fact, FAK is involved in cell proliferation and cell motility by performing signal transduction through integrins. FAK-related non-kinase (FRNK) has been found to be an inhibitor of FAK. As the expression level of FRNK in the cell is very low, the study of FRNK has been preferentially performed by gene overexpression, up to the present, and the role of constitutive FRNK in cells remains unclear. We hypothesized that FRNK is involved in the adhesion of cells to the extracellular matrix (ECM) and investigated the expression of FRNK by time kinetic analysis shortly after cell seeding. We found that FRNK expression was significantly increased in the cells during the early stage of cell adhesion to the ECM. These data indicated that FRNK plays an important role in cell adhesion during the very early stages of cell culture.