Liver transplant recipients: portable duplex US with correlative angiography

Twenty patients, aged 4 months to 58 years, were evaluated for liver transplantation by duplex sonography, and 15 transplantations were completed; 42 postoperative examinations were performed. Sonographic findings were correlated with seven preoperative and five postoperative angiographic evaluations. Preoperative duplex US findings included tumors, portal vein occlusion, varices, biliary obstruction, and variant vascular anatomy. Postoperative findings included hepatic artery occlusion, portal vein occlusions (one with cavernous transformation), portal vein stenosis, biliary obstruction, intrahepatic and extrahepatic fluid collections, and air in the portal vein due to ischemic bowel. Use of angiography allowed confirmation of the vascular abnormalities and demonstrated evidence of rejection in patients with normal Doppler waveforms. Duplex sonography is a valuable portable technique for evaluating these patients and can be used in triage of patients requiring angiography.     

Trabecular bone mineral density measurement in vivo: use of the ratio of coherent to Compton-scattered photons in the calcaneus

Trabecular bone mineral density (TBMD) was measured in vivo in the calcaneus by a new method that uses the ratio of coherent to Compton-scattered photons arising from irradiation of a small volume of trabecular bone by a gamma ray source with highly collimated geometry. TBMD values for healthy men (22-77 years) were in the range of 180-357 mg/ml, and values for healthy women (18-73 years) were in the range of 160-321 mg/ml. In contrast, values in a small group of paraplegics were in the range of 90-199 mg/ml.     

单克隆抗体BDI-I导向的阿霉素白蛋白毫微球对人膀胱癌细胞的特异杀伤活性

用化学偶联法将抗人膀胱癌单克隆抗体分子偶联到阿霉素白蛋白毫微球上,构建了一个有靶向杀伤性的免疫毫微球,即:阿霉素白蛋白载单克隆抗体毫微球(ADR-NP-Ab)。改变阿霉素毫微球和单克隆抗体的反应分子比,确定了制备该免疫毫微球的最佳条件。经免疫荧光检测及显微照像分析证明,免疫毫微球可有效地和人膀胱癌细胞结合。体外杀伤试验表明,此免疫毫微球对靶细胞EJ有高度特异杀伤活性,而对无关的人直肠癌Lovo细胞则无明显作用。     

Thymidylat-de novo-Synthese,salvage pathway und DNS-Synthese während der Differenzierung von Erythroblasten

Zusammenfassung Die de novo-Synthese von Thymidinmonophosphat in erythropoetischen Knochenmarkzellen einer normalen Ratte und einer gesunden Versuchsperson wird durch in vitro-Inkubation mit Fluordesoxyuridin FUdR) blockiert. Zur Aufrechterhaltung einer normalen DNS-Synthesegeschwindigkeit erhöhen die Zellen kompensatorisch die Inkorporationsrate von¹⁴C-Thymidin aus dem Inkubationsmedium. Dadurch können der Anteil des salvage pathway an der Thymidylat-Synthese und die DNS-Synthesegeschwindigkeit mit Hilfe einer quantitativen autoradiographischen Methode in den einzelnen Reifungsstufen bestimmt werden. Der Anteil des salvage pathway unter 10^–5m Thymidin liegt bei der Ratte mit 40 bis 80% erheblich höher als beim Menschen mit 28 bis 37%. In beiden Fällen findet sich bei den polychromatischen Erythroblasten sowohl ein geringerer Anteil des salvage pathway an der Thymidinmonophosphat-Synthese als auch eine deutlich geringere DNS-Synthesegeschwindigkeit als bei den Proerythroblasten und basophilen Erythroblasten.

---

Summary The de novo synthesis of thymidinemonophosphate is blocked by in vitro incubation with fluorodeoxyuridine (FUdR) of erythropoietic bone marrow cells of a normal rat and a healthy individual. The cells maintain a normal DNA synthesis rate under these conditions by increasing the incorporation rate of¹⁴C-thymidine out of the medium. Thereby the participation of the salvage pathway in the synthesis of thymidilate as well as the DNA synthesis rate can be evaluated. This is by means of a quantitative autoradiographic method in the different erythropoietic maturation stages. The participation of the salvage pathway in the erythropoietic cells of the rat with 10^–5m¹⁴C-thymidine amounts to 40 to 80%. This is considerably higher than in the human cells where a participation of 28 to 37% is found. The salvage pathway activity as well as the DNA synthesis rate is markedly lower in polychromatic erythroblasts than in proerythroblasts and basophilic erythroblasts of both rat and man.

---

---

Studie im Rahmen des Assoziationsvertrages EURATOM-GSF für Hämatologie Nr. 031 641 BIAD. Unterstützt von der Deutschen Forschungsgemeinschaft: SFB 51/5.