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991.
目的:探讨锌指E-盒结合同源异形盒-1 (ZEB1)在肺鳞状细胞癌组织中的表达,分析ZEB1 mRNA和蛋白的表达水平与肺鳞状细胞癌侵袭及转移的关系。方法:选择45例肺鳞状细胞癌行外科手术切除的患者,根据所取组织与肿瘤的距离不同分为肺鳞状细胞癌组织和癌旁正常肺组织,采用RT-PCR和Western blotting方法检测肺鳞状细胞癌组织和癌旁正常肺组织中ZEB1 mRNA和蛋白的表达情况,并分析肺鳞状细胞癌不同临床病理特征ZEB1 mRNA和蛋白的表达差异;构建ZEB1特异性siRNA载体,感染肺癌NCI-H2170细胞,设未转染对照组、转染照组和ZEB1 siRNA转染组,应用Western blotting方法从蛋白质水平检测各组干扰质粒对ZEB1基因的干扰效果,通过Transwell侵袭小室观察各组NCI-H2170细胞侵袭能力的变化。结果: ZEB1 mRNA在肺鳞状细胞癌组织中的阳性表达率显著高于癌旁正常肺组织(P<0.05);在淋巴结转移、远处转移阳性者肺癌组织中的阳性表达率显著高于其在淋巴结转移、远处转
移阴性者肺癌组织中的表达(P<0.05);但ZEB1 mRNA的表达与肺鳞状细胞癌患者的性别、年龄及肿瘤大小及分化程度无关(P>0.05)。肺鳞状细胞癌组织中ZEB1蛋白的表达与ZEB1 mRNA的表达结果类似,ZEB1蛋白在肺鳞状细胞癌组织中的表达量显著高于癌旁正常肺组织(P<0.05);在淋巴结转移和远处转移阳性者癌组织中的表达显著高于其在淋巴结转移和远处转移阴性者癌组织中的表达(P<0.05)。下调NCI-H2170细胞中ZEB1蛋白的表达后,NCI-H2
170细胞的侵袭及转移能力明显降低(P<0.05)。结论:ZEB1在肺鳞状细胞癌组织中高表达,表达变化与肺鳞状细胞癌的病理特征密切相关;运用RNA干扰技术有效沉默NCI-H2170细胞的ZEB1基因后,可显著降低NCI-H2170细胞的侵袭及转移能力,提示ZEB1在肺癌的发生发展中起重要作用,抑制ZEB1的表达可能成为一种治疗肺癌的方法。
相似文献
移阴性者肺癌组织中的表达(P<0.05);但ZEB1 mRNA的表达与肺鳞状细胞癌患者的性别、年龄及肿瘤大小及分化程度无关(P>0.05)。肺鳞状细胞癌组织中ZEB1蛋白的表达与ZEB1 mRNA的表达结果类似,ZEB1蛋白在肺鳞状细胞癌组织中的表达量显著高于癌旁正常肺组织(P<0.05);在淋巴结转移和远处转移阳性者癌组织中的表达显著高于其在淋巴结转移和远处转移阴性者癌组织中的表达(P<0.05)。下调NCI-H2170细胞中ZEB1蛋白的表达后,NCI-H2
170细胞的侵袭及转移能力明显降低(P<0.05)。结论:ZEB1在肺鳞状细胞癌组织中高表达,表达变化与肺鳞状细胞癌的病理特征密切相关;运用RNA干扰技术有效沉默NCI-H2170细胞的ZEB1基因后,可显著降低NCI-H2170细胞的侵袭及转移能力,提示ZEB1在肺癌的发生发展中起重要作用,抑制ZEB1的表达可能成为一种治疗肺癌的方法。
相似文献
992.
目的探讨创伤性膈疝的诊断及治疗方法。方法回顾性分析24例创伤性膈疝患者的致伤原因及诊断、治疗过程。结果 24例创伤性膈疝患者均行手术治疗,治愈22例,死亡2例,治愈率91.7%。结论对胸腹部创伤患者详细询问受伤情况,全面体格检查,根据X线、CT和临床特点多能得到早期诊断,及时手术治疗是提高治愈率的关键。 相似文献
993.
An open-label, single-dose, randomized, crossover study was carried out in 20 Chinese healthy male subjects to compare the pharmacokinetics of 2 cefaclor (CAS 53994-73-3) formulations after administration of a single 250 mg dose of each drug with a 1-week wash-out period. Blood samples were collected before and with 6 h after drug administration. Plasma concentrations were determined by high-performance liquid chromatography (HPLC) with UV detector. 2 formulations were evaluated using the following pharmacokinetic parameters: AUC0-t, Cmax and tmax was analyzed nonparametrically. The 90% confidence interval (CI) of the ratios (teat/reference) of log-transformed AUC0-t and Cmax fell within the bioequivalence acceptance range of 80-125%. The results showed that the 90% CI of the ratios of AUC0-t and Cmax were 105.1% (101.0-109.4%) and 92.4% (82.5-103.4%), respectively, which therefore could conclude 2 oral cefaclor capsule formulations of cefaclor are bioequivalent. Both treatments showed similar tolerability and safety. 相似文献
994.
Recent clinical studies on human skin indicated that in vivo multi-harmonic generation microscopy (HGM) can achieve sub-micron resolution for histopathological analysis with a high penetration depth and leave no energy or photodamages in the interacted tissues. It is thus highly desired to apply HGM for in vivo mucosa histopathological diagnosis. In this paper, the first in vivo optical virtual biopsy of human oral mucosa by using epi-HGM is demonstrated. We modified an upright microscope to rotate the angle of objective for in vivo observation. Our clinical study reveals the capability of HGM to in vivo image cell distributions in human oral mucosa, including epithelium and lamina propria with a high penetration depth greater than 280 μm and a high spatial resolution better than 500 nm. We also found that the third-harmonic-generation (THG) contrast on nucleus depends strongly on its thicknesses, in agreement with a numerical simulation. Besides, 4% acetic acid was found to be able to enhance the THG contrast of nucleus in oral mucosa, while such enhancement was found to decay due to the metabolic clearance of the contrast enhancer by the oral mucosa. Our clinical study indicated that, the combined epi-THG and epi-second-harmonic-generation (SHG) microscopy is a promising imaging tool for in vivo noninvasive optical virtual biopsy and disease diagnosis in human mucosa. 相似文献
995.
Purpose: Spread of seizure activity outside the frontal lobe due to cortico‐cortical connections can result in alteration in the cortex beyond the frontal lobe in children with intractable frontal lobe epilepsy (FLE). The aim of this study was to identify regions of reduced cortical thickness in children with intractable FLE. Methods: High‐resolution volumetric T1‐weighted imaging was performed on 17 children with FLE, who were being evaluated for epilepsy surgery, and 26 age‐matched healthy controls. The cortical thickness of 12 patients with left FLE and 5 patients with right FLE was compared to controls. The clusters of cortical thinning were regressed against age of seizure onset, duration of epilepsy, seizure frequency, and number of medications. Key Findings: In children with left FLE, cortical thinning was present in the left superior frontal, paracentral, precuneus, cingulate, inferior parietal, supramarginal, postcentral, and superior temporal gyri, as well as in the right superior and middle frontal, medial orbitofrontal, supramarginal, postcentral, banks of superior temporal sulcus, and parahippocampal gyri. In children with right FLE, cortical thinning was present in the right precentral, postcentral, transverse temporal, parahippocampal, lingual, and lateral occipital gyri, as well as in the left superior frontal, inferior parietal, postcentral, superior temporal, posterior cingulate, and lingual gyri. In children with left FLE, following exclusion of one outlier, there was no significant association between age at seizure onset, duration of epilepsy, seizure frequency and number of medications with clusters of cortical thinning. In children with right FLE, age at seizure onset, duration of epilepsy, frequency of seizures, and number of medications were not associated with clusters of cortical thinning within the right and left hemispheres. Significance: Cortical changes were present in the frontal and extrafrontal cortex in children with intractable FLE. These changes may be related to spread of seizure activity, large epileptogenic zones involving both frontal and extrafrontal lobes, and development of secondary epileptogenic zones that over time lead to cortical abnormality. Further studies correlating cortical changes with neurocognitive measures are needed to determine if the cortical changes relate to cognitive function. 相似文献
996.
997.
Pengfei Zheng Kai Tang Robert Lee Chenbo Ji Gang Lin Xinhua Pan Zhiqun Zhang Yue Lou 《Journal of orthopaedic science》2011,16(2):165-170
Background
Management of developmental dysplasia of the hip in older children remains controversial. The objective of this study was to evaluate the results of surgical treatment of developmental dysplasia of the hip in children older than 10 years. 相似文献998.
Huiying Liu Boye Fang Jieling Chan Vivian WQ Lou 《Geriatrics & Gerontology International》2019,19(10):972-976
999.
Song I Borland J Min S Lou Y Chen S Patel P Wajima T Piscitelli SC 《Antimicrobial agents and chemotherapy》2011,55(7):3517-3521
Dolutegravir (DTG) is an unboosted, once-daily integrase inhibitor currently in phase 3 trials. Two studies evaluated the effects of etravirine (ETR) alone and in combination with ritonavir (RTV)-boosted protease inhibitors (PIs) on DTG pharmacokinetics (PK) in healthy subjects. DTG 50 mg every 24 h (q24h) was administered alone for 5 days in period 1, followed by combination with ETR at 200 mg q12h for 14 days in period 2 (study 1) or with ETR/lopinavir (LPV)/RTV at 200/400/100 mg q12h or ETR/darunavir (DRV)/RTV at 200/600/100 mg q12h for 14 days in period 2 (study 2). PK samples were collected on day 5 in period 1 and day 14 in period 2. All of the treatments were well tolerated. ETR significantly decreased exposures of DTG, with geometric mean ratios of 0.294 (90% confidence intervals, 0.257 to 0.337) for the area under the curve from time zero until the end of the dosage interval (AUC(0-τ)), 0.484 (0.433 to 0.542) for the observed maximum plasma concentration (C(max)), and 0.121 (0.093 to 0.157) for the plasma concentration at the end of the dosage interval (C(τ)). ETR combined with an RTV-boosted PI affected the exposure of DTG to a lesser degree: ETR/LPV/RTV treatment had no effect on the DTG plasma AUC(0-τ) and C(max), whereas the C(τ) increased by 28%. ETR/DRV/RTV modestly decreased the plasma DTG AUC(0-τ), C(max), and C(τ) by 25, 12, and 37%, respectively. Such effects of ETR/LPV/RTV and ETR/DRV/RTV are not considered clinically relevant. The combination of DTG and ETR alone should be avoided; however, DTG may be coadministered with ETR without a dosage adjustment if LPV/RTV or DRV/RTV is concurrently administered. 相似文献
1000.