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BRUNA PALOMA DE OLIVEIRA CARLOS MENEZES AGUIAR ANDRéA CRUZ C?MARA MIRACY MUNIZ DE ALBUQUERQUE ANA CRISTINA REGIS DE BARROS CORREIA MONICA FELTS DE LA ROCA SOARES 《Acta stomatologica Croatica》2015,49(4):294-303
Objective
This in vitro study aimed to evaluate the efficacy of the disinfection of root canal systems carried out with ReciprocTM and ProTaper UniversalTM systems using 1% sodium hypochlorite (NaOCl).Methods
Forty human single-rooted mandibular premolars were infected with Enterococcus faecalis, Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans, and twenty were not infected. The specimens were randomly divided into 6 groups (n = 10): Group 1: ProTaper UniversalTM + 1% NaOCl; Group 2 (positive control): ProTaper UniversalTM + saline; Group 3 (negative control without microorganisms): ProTaper UniversalTM + saline; Group 4: ReciprocTM + 1% NaOCl; Group 5 (positive control): ReciprocTM + saline; Group 6 (negative control without microorganisms): ReciprocTM + saline. Samples were collected before and after the completion of specific treatments, and plated in specific media cultures. The Fisher exact test was used for the statistical analysis of differences in terms of presence or absence of microbial growth among groups. For all tested pathogens, significant differences (p < 0.001) were verified between the instrumentation systems used.Results
ProTaper UniversalTM associated with 1% NaOCl completely eliminated all microorganisms. Microbial growth, however, was observed when ReciprocTM was used associated with 1% NaOCl.Conclusion
According to the protocol executed for this study, the ReciprocTM system associated with 1% NaOCl was not able to completely eliminate E. faecalis, P. aeruginosa, S. aureus and C. albicans from the root canal systems.Key words: endodontic treatment, Enterococcus faecalis, single-file instrumentation, sodium hypochlorite 相似文献12.
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Málnási-Csizmadia A Dickens JL Zeng W Bagshaw CR 《Journal of muscle research and cell motility》2005,26(1):31-37
The myosin II motor from Dictyostelium discoideum has been engineered to contain single tryptophan residues at strategic locations to probe movements of switch 1 and switch
2. The tryptophan residue at W501 probes movement of the relay helix and indirectly reports on switch 2 movement. This probe
suggests that there is an equilibrium between the switch 2 open- and closed-states when the γ-phosphate position is occupied.
Actin does not appear to greatly affect this equilibrium directly, but has indirect influence via switch 1. The latter region
has been probed by introducing tryptophan residues at positions 239 and 242. The kinetics of the actomyosin ATPase in solution
is discussed with respect to recent crossbridge models based on high-resolution crystal structures.
To whom correspondence should be addressed: Tel.: +44-(0)-116-229-7048; Fax: +44-(0)-116-229-7018; E-mail: crb5@le.ac.uk 相似文献
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Ana Beatriz Mori LIMA Lara Stefania Netto de Oliveira LE?O-VASCONCELOS Dayane de Melo COSTA Larissa Oliveira Rocha VILEFORT Maria Cláudia Dantas Porfírio Borges ANDRé Maria Alves BARBOSA Marinésia Aparecida PRADO-PALOS 《Revista do Instituto de Medicina Tropical de S?o Paulo》2015,57(6):513-514
This cross-sectional study, performed in an oncology hospital in Goiania, aimed to
characterize the prevalence of oral colonization and antimicrobial susceptibility of
Pseudomonas spp. isolated from the saliva of healthcare workers. Microorganisms were
subjected to biochemical tests, susceptibility profile, and phenotypic detection. Of
76 participants colonized with Gram negative bacilli, 12 (15.8%) harbored Pseudomonas
spp. Of all isolates, P. aeruginosa (75.0%), P. stutzeri (16.7%), and P. fluorescens
(8.3%), were resistant to cefoxitin, and therefore likely to be AmpC producers. The
results are clinically relevant and emphasize the importance of surveillance to
minimize bacterial dissemination and multiresistance. 相似文献
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