眼前房转移性肺癌一例

患者男,５２岁,因发现复视、伴右眼视力下降１周就诊。眼科检查：视力右０．８,左１．２。右眼无充血,角膜透明,前房上方偏外见一灰白色肿块生长,约６ｍｍ×４ｍｍ×２ｍｍ大小,裂隙图１右眼前房转移性肺癌灯下可见肿块表面小血管生长,根部起源于上方前房角,游离...     

转化生长因子β2诱导人小梁细胞凋亡的体外研究

目的　观察转化生长因子 β2 (transforminggrowthfactor β2 ,TGF β2 )是否能诱导体外培养人眼小梁细胞 (humantrabecularmeshworkcells,HTMC)发生凋亡。方法　将体外培养的第 3～ 5代人眼小梁细胞分为对照组和实验组 ,实验各组分别经 0 .32、1.0 0、3.2μg·L-1TGF β2 作用 4 8h后 ,分别用透射电镜、TUNEL法和流式细胞术检测细胞凋亡的诱导情况。结果　透射电镜观察发现凋亡细胞典型的形态学变化———核染色质凝集边集 ,凋亡小体形成等。TUNEL法检出发生DNA片段化的HTMC。经流式细胞术定量分析 ,不同浓度TGF β2 处理的HTMC凋亡细胞百分数分别为 ( 2 .79± 0 .4 4 ) %、( 4 .4 3±1.17) %、( 9.6 0± 2 .0 5 ) % ,其中 1、3.2 μg·L-1TGF β2 处理组与对照组 ( 1.4 1± 0 .34) %相比分别有显著差异和极显著差异。结论　TGF β2 能诱导体外培养HTMC发生凋亡 ,推测其可能参与了原发性开角型青光眼患者和正常人衰老过程中HTMC数目的减少。     

兔眼结膜下注射5-氟尿嘧啶多相脂质体的药代动力学研究

为探讨5-氟尿嘧啶（5-Fu）脂质体应用于临床青光眼手术的可能性，应用卵磷脂胆固醇等原料，通过熔溶法自制成5-Fu多相脂质体，其包封率为52％，脂质体粒径＜2μm者占97.2％，对冷热均有较好稳定性。将0.5ml多相脂质体制剂（内含氯标5-Fu5mg）注射于家兔球结膜下，在注射后12、24、48、72及96小时利用闪烁法测定距注射处180°的结膜，巩膜及结膜巩膜筋膜合并的整块组织的含药量，发现上述各组织样本的5-Fu浓度均不低于0.2μg/g（成纤维细胞增殖的半数抑制量）。结果提示有可能以5-Fu多相脂质体代替5-Fu水溶液应于青光眼手术，以减少注射的…     

5－氟尿嘧啶对体外培养牛眼小梁细胞的毒性研究

目的探讨５－氟尿嘧啶（５－ｆｌｕｏｒｏｕｒａｃｉｌ，５－Ｆｕ）在临床应用中对小梁细胞的毒性损伤。方法在体外培养牛眼小梁细胞，从细胞的形态、结构、生存及吞噬功能等方面观察５－Ｆｕ对牛眼小梁细胞的影响。结果５－Ｆｕ对牛眼小梁细胞的安全浓度为１×１０－６ｇｍｌ－１。结论结合５－Ｆｕ在兔眼前房的药代动力学研究资料，推论目前在人眼应用的剂量，不致对小梁细胞造成损伤。     

先天性青光眼50例(90只眼)临床分析

本文对1963～1978年住院的先天性青光眼患者50例进行了远期随访及临床分析。发现此病占同期全部青光眼住院患者的5.2％。其中36例为男性、14例女性,双眼40例、单眼10例。由于角膜大而引起家长注意者最多,因畏光流泪而发现者反而较少。曾测角膜直径者43眼,其中有16眼大于14毫米汞柱。86眼做手术98     

转移因子在眼科的应用

转移因子是胸腺依赖淋巴细胞(简称T细胞)产生的一种淋巴激活素,可存在于完整的淋巴细胞内或高度提纯的淋巴细胞浸出液中。在T细胞表面上有与一定的抗原相应的特异性受体。当抗原与受体相作用时,T细胞即被激活,而产生转移因产及其他淋巴激活素(因子)。局时在淋巴细胞衷面还有一种与转移因子相应的微型受体,故藉助于转移因子还可以激活其也未与抗原接触的淋巴细胞。如将转移因子注入同种其他个体,也可以使其增加细胞免疫能力。     

Inhibitory Effect of Dexamethasone on TGF-β1 Expression of Rabbit Ciliary Pigment Epithelia Cultured in Vitro

As is well-known,transforming growth fac-tor-beta1(TGF-β1)is a multi-functioning factorwith sti mulating or inhibitory effects to majority ofcells in the body.In ocular,many cell types ex-pressed TGF-β1andits receptors,such as cornea,lens,trabecular meshwork and retinal cells.Par-ticularly,TGF-β1has been believed to play a cru-cial roleinthe developing of eye embryo[1].Due tothe double-direction regulating effect to differentcell types,the exact mechanism and regulatorypattern are uncle…     

Apoptosis of Human Trabecular Meshwork Cells Induced by Transforming Growth Factor-p2 in vitro

Summary: Whether transforming growth factor-β2 (TGF-β2) induces apoptosis of human trabecular meshwork cells was investigated in vitro. Cultured 3-5 passage human trabecular meshwork cells were treated with 0 (control), 0.32, 1, 3.2 ng/ml TGF-β2 for 48 h and divided into control group and experimental group. The apoptosis of human trabecular meshwork cells was examined by transmisson electron microscopy, TUNEL technique and flow cytometry. The results showed characteristic morphologic changes of apoptotic cells were observed under transmission electron microscopy.DNA fragmentation of human trabecular meshwork cells was found by TUNEL technique. Quantitative analysis of flow cytometry showed that percentages of apoptotic human trabecular meshwork cells were (2.79±0.44) %, (4.43±1.17) % and (9. 60±2.05) % respectively with different concentrations [1 ng/ml (P＜0. 05), 3.2 ng/ml (P＜0.01)] of TGF-β2 with the difference being significant between experimental group and control group[(1. 41±0.34) %]. It was concluded that TGF-β2 can induce apoptosis of human trabecular meshwork cells in vitro and may be involved in the decrease of trabecular meshwork cells in the patients with primary open angle glaucoma and aging of normal people.     

伽玛刀治疗眼内肿瘤的近期疗效观察（附3例报告）

报告了３例眼内肿瘤患者用伽玛刀治疗的初步结果。术后３～６个月夏查，１例肿瘤消失，２刑肿瘤缩小。伽玛刀与眼科手术相比，具有不需摘除眼球，保存面部美观，甚至保存视力等优点。