ATR inhibition sensitizes liposarcoma to doxorubicin by increasing DNA damage

Liposarcomas account for approximately 20% of all adult sarcomas and have limited therapeutic options outside of surgery. Inhibition of ataxia-telangiectasia and Rad3 related protein kinase (ATR) has emerged as a promising chemotherapeutic strategy in various cancers. However, its activation, expression, and function in liposarcoma remain unkown. In this study, we investigated the expression, function, and potential of ATR as a therapeutic target in liposarcoma. Activation and expression of ATR in liposarcoma was analyzed by immunohistochemistry, which was further explored for correlation with patient clinical characteristics. ATR-specific siRNA and the ATR inhibitor VE-822 were applied to determine the effect of ATR inhibition on liposarcoma cell proliferation and anti-apoptotic activity. Migration activity and clonogenicity were examined using wound healing and clonogenic assays. ATR (p-ATR) was overexpressed in 88.1% of the liposarcoma specimens and correlated with shorter overall survival in patients. Knockdown of ATR via specific siRNA or inhibition with VE-822 suppressed liposarcoma cell growth, proliferation, migration, colony-forming ability, and spheroid growth. Importantly, ATR inhibition significantly and synergistically enhanced liposarcoma cell line chemosensitivity to doxorubicin. Our findings support ATR as critical to liposarcoma proliferation and doxorubicin resistance. Therefore, the addition of ATR inhibition to a standard doxorubicin regimen is a potential treatment strategy for liposarcoma.     

异佛尔酮二异氰酸酯免疫毒性的实验研究

本文选用小鼠溶血空斑形成试验研究了异佛尔酮二异氰酸酯(IPDI)对Bal/C小鼠脾细胞的作用,选用ANAE染色法观察了IPDI对SD大鼠外周血T细胞的影响,并采用体外培养方法研究了IPDI对人外周血T细胞在PHA刺激下增殖转化的影响。结果表明,IPDI对小鼠脾脏抗体生成细胞的数量和功能均有抑制作用,并呈现出剂量-反应关系;而对细胞免疫反应在大鼠和人均表现为双向作用。     

敦煌医学及其文化内涵探析

探讨了敦煌医学的文化内涵,提出其特色主要表现在地域性、开放性、多样性、文学性与艺术性,旨在为敦煌医学及其文化的研究提供思路。     

Effects of tooth rotation on neighboring teeth and periodontal tissues

In the present experiment,we investigate the effects of orthodontic tooth rotation onneighboring teeth and periodontal tissues.The second maxillary incisors of 16 randomly selecteddogs were subjected to rotation at a magnitude of 100 grams and a moment of 550 g·mm in a du-ration of 3 to 56 days.Then sections were prepared for histological examination.The findings re-vealed that,due to tooth rotation,neighboring teeth were drifted and rotated(82%),and therewere disarrangement and hyalinization of the periodontal ligament(88%),remodeling of the pe-riodontium(86%)and root resorption(32%)were also observed.These results demonstratethat tooth rotation may exert marked effects on neighboring teeth and periodontal tissues,whichmay be of important clinical significance.     

A study of the rotation rate of single-rooted teeth in dogs

Sixteen one-year-old dogs were used in our experiment.We rotated the maxillary secondincisors of the dogs,applying a force of 100g and a moment of 550g·mm.The duration of theexperiment varied from 72h to 8 weeks.Activation was performed once every 2 weeks and the impressions were taken in order to measure the angles of rotation.The results of our experiment showthat the rotation rate of single-rooted tooth in dogs is 3.04/week.The graph shows the form ofgradual stairs.This paper introduces a new specialized experimental apparatus for tooth rotation.The angles of rotation are measured geometrically.     

翻转课堂在骨科教学中的可行性及必要性分析

翻转课堂目前已逐渐成为教育领域的关注焦点,但在医学教育领域内,目前该技术应用不多。该文介绍了翻转课堂的由来、特点,重点讨论了在骨科教学中应用的可行性、必要性,并分析了目前推广应用该技术的难点和障碍所在,旨在推动医学教育的不断发展。     

老年性上消化道疾病的临床及x线特点

本文报告144例老年性上消化道疾病,并与112例青年病人进行比较。初步探讨老年人上消化道疾病在临床及 x 线上有以下特点:1.病史长,平均12.3年;2.一般性消化道症状多,典型症状少,并且消化道肿瘤、胃下垂,憩室明显增多;3.并发症多,为36.3%;4.x 线表现功能性改变、粘膜破坏、胃壁僵硬多见而明显。     

Role of arachidonoylethanolamine in blood pressure regulation in volume-resistant patients on peritoneal dialysis

In this study we explored the possible role of arachidonoylethanolamine (AEA) in regulating blood pressure in patients on continuous ambulatory peritoneal dialysis (CAPD). One hundred and five patients on CAPD were enrolled. Volume status was evaluated by the overhydration (OH) value obtained by bioimpedance analysis. OH?<?2.0?kg was defined as normal volume (NV) and OH????2.0?kg as high volume (HV). Home mean systolic blood pressure <130?mmHg was defined as controlled hypertension (CHT) and ??130?mmHg as uncontrolled hypertension (UHT). The patients were divided into four subgroups: (1) controlled hypertension with normal volume (CHT-NV), (2) controlled hypertension with high volume (CHT-HV), (3) uncontrolled hypertension with normal volume (UHT-NV), and (4) uncontrolled hypertension with high volume (UHT-HV). AEA was measured by ultra performance liquid chromatography-tandem mass spectrometry. AEA was significantly higher in the HV group as compared with the NV group (P?<?0.05). In addition, AEA was also significantly higher in the CHT-HV group as compared with the UHT-NV group (P?<?0.05). These results may suggest a compensatory function of AEA and TRPV1 pathway to lower blood pressure during volume expansion in CAPD patients.     

华南地区某眼科医院的感染性角膜炎病原菌谱特征

目的 探讨华南地区某眼科医院感染性角膜炎的病原菌谱及其特点。方法 回顾性系列病例研究。选择2013年7月1日至2017年12月31日在中山大学中山眼科中心进行角膜取材和微生物检测的角膜感染患者3 914例。表面麻醉后刮取病灶边缘的病灶组织,进行细菌、真菌培养及鉴定。培养结果阳性的细菌采用生化比色或质谱进行种属鉴定;真菌依据形态学进行种属鉴定。各季节菌谱阳性率差异比较采用卡方检验。结果 感染性角膜炎患者中,男2 468例（63.1%）,女1 446例（36.9%）,年龄（49.5±16.8）岁。病原菌培养阳性的患者共1 673例（42.7%）,其中单纯细菌749例（44.8%）,单纯真菌849例（50.7%）,混合感染75例（4.5%）。最常见的细菌是葡萄球菌属（20.1%）,其次是假单胞菌属（7.5%）;最常见的真菌是镰刀菌属（25.5%）,其次是曲霉菌属（10.3%）。细菌的阳性率在5-8月明显高于1-2月和9-12月（P<0.05）,真菌的阳性率在5-6月明显低于1-2月和9-12月（P<0.05）。结论 在华南地区一所三甲眼科医院的感染性角膜炎中,真菌感染略高于细菌感染,且细菌和真菌感染存在季节性差异。