Effect of “Re Du Qing” on the activation,proliferation and membrane fluidity of lymphocytes

Summary Effects of Chinese Medicinal Preparation “Re Du Qing” (RDQ) on the activation, proliferation and membrane fluidity of T lymphocytes from human peripheral blood Were studied by means of³H-TdR incorporation and DPH fluorescence polarization. The results showed that “RDQ” can:1) significantly inhibit the activation of T lymphocytes; 2) restrain the proliferation of activated T lymphoblasts in the presence of exogenous interleukin-2 (IL-2); and 3) increase the membrane fluidity of T lymphocytes and antagonize the decreased fluidity of lymphocyte membrane mediated by Con A or PHA. The functional abnormalities of T lymphocytes in some autoimmune diseases such as arthritis and the usefulness of RDQ in the treatment of these diseases were also discussed.     

中枢和外周脑钠素(BNP)调节醛固酮分泌的不同作用

将BNP和AT-Ⅱ、ACTH AVP单独或BNP与这3种肽分别合并在大鼠icv或iv注入后观察血Ald浓度的变化。实验结果表明:①iv给予AVP(5μg/3ml·h~(-1))、ACTH(5μg/3ml·h~(-1))和AT-Ⅱ(5 v.g/3 ml·h(-1)),1h后均能增加血Ald的浓度。iv给予BNP(5μg/3ml·h(-1))能明显抑制AVP和ACTH的刺激作用,而不影响AT-Ⅱ的刺激作用。②icv给予BNP(2 Vg/10 pl NS)能降低血Aid浓度。icv给予AVP(2μg/10μl NS)能增加血Aid浓度,但ACTH(2μg/10μl NS)和AT-Ⅱ(2μg/10μl NS)无此种作用。但如脑室同时给予BNP(2μg/10μlNS)却可明显刺激Aid分泌。③icv注入BNP对外周注入的3种多肽的刺激作用无任何影响。从上述的实验结果可看出:脑内BNP可“反常”地增加AT-Ⅱ、ACTH和AVP对Ald分泌的刺激作用,与外周抑制AVP,ACTH的刺激作用不同。而脑内BNP不影响这3种多肽的外周刺激作用。结论是脑内BNP以其独特的方式调节Ald的分泌,控制水盐代谢。     

显微外科治疗颅内肿瘤65例临床分析

目的：介绍65例颅内肿瘤显微外科手术治疗经验；方法：本组患者65例，根据肿瘤部位、大小、性质，选择合适的手术入路，采用显微手术切除肿瘤；结果：全切53例，部分切除12例，无手术死亡；术后并发视神经麻痹1例，面神经并动眼神经麻痹1例，三叉神经麻痹1例，舌咽神经麻痹1例，偏瘫5例，无其他手术并发症；结论：正确的手术思路、合适的手术入路是手术成功的基础；采用显微外科手术技术是提高全切率、减少并发症的关键。     

口服二巯基丁二酸治疗狗路易氏剂中毒

为证实口服二巯基丁二酸（ＤＭＳＡ）治疗路易氏剂中毒的效价，以狗为实验动物，进行实验治疗研究，狗口服ＤＭＳＡ（４０～１２０ｍｇ／ｋｇ）后，２周内未见到有关的生理，生化指标明显变化，该药毒性较小。ＤＭＳＡ可在肠道内较好地被吸收，与静脉注射途径比较，口服组血药浓度上升慢，下降亦慢，可在体内维持较长时间，ＤＭＳＡ在体内促排砷的作用与静脉注射同剂量ＤＭＳＡ（钠盐）相当。狗口服ＤＭＳＡ４０ｍｇ／ｋｇ可对抗路易     

抗Ⅲ型前胶原肽单克隆抗体的制备及初步应用

制备出两株分泌抗ＰⅢＰ（Ⅲ型前胶原肽）单克隆抗体的杂交瘤细胞株。竞争抑制ＥＬＩＳＡ试验表明：两株单抗与ＰＣＩ（Ⅰ型前胶原）及ＣⅢ（Ⅲ型胶原）无交叉反应。Ｄｏｔ－ＥＬＩＳＡ示两株单抗钧针对ＰⅢＰ构象决定簇。应用所制备的单抗对人肝细胞癌组织及大鼠、小鼠成纤维母细胞进行免疫定位。结果显示两种成纤维母细胞呈明显膜阳性；肝癌细胞浆内呈粗颗粒状染色，提示肝癌细胞具有合成Ⅲ型前胶原的能力。     

珊瑚型人工髋关节置换术的临床随访及松动原因的分析

自１９８３年至今应用珊瑚型人工髋关节置换术共５４例６０髋。其中人工全髋关节置换术３４例４０髋，人工股骨头置换术２０例２０髋。在５４例６０髋珊瑚型人工髋关节置换术中，有３７髋随访６个月～１３年，平均随访时间约７年，疗效满意率为８５．１％，其中假体松动４例占１４．８％，髋臼松动２例７．４％。假体松动原因是假体与股骨负重界面之间没有达到稳定接触，假体与髓腔形状不相匹配，修整髓腔松质骨时，髓腔扩大器应比假体小一号，避免假体与界面存留缝隙。对于髋臼发育不良的患者应避免髋关节旋转中心向外侧移位，应向内上方加深髋臼以减少水平移位距离，其次髋臼植骨加盖勿在负重区。     

胜利黄岛原油深度电脱盐的研究：Ⅰ.破乳剂组成的影响

根据胜利黄岛原油的性质，在筛选了几十种破乳剂的基础上，选择了酚醛树脂和环氧环烷，环氧乙烷的嵌段共聚物作破乳剂，探讨了破乳剂组成对原油脱水，脱盐以及界面张力的影响，通过静态脱盐，脱水试验，得到了适合黄岛原油的最佳破乳剂组成。     

一种中西药复方制剂(PESM)对大鼠实验性肾结石形成的抑制作用

用乙二醇法诱导大鼠产生草酸钙结石，用45~Ca示踪测定法了解中草药复方制剂（PESM）对草酸钙结石形成的抑制作用。结果显示，实验组肾脏放射性强度明显减少（实验组3.47±3.52cpm／mg，对照组10.89±14.24cpm／mg）。干肾重量明显下降（实验组306.0±47.0mg，对照组343.1±59.4mg），说明PESM能明显抑制肾脏草酸钙结石的形成。     

Key role for pregnenolone in combination therapy that promotes recovery after spinal cord injury.

Controlled compressive injury to rat spinal cord was chosen to test therapies that might attenuate the progression of tissue destruction and locomotor deficits that characteristically occur after spinal injury. A highly significant reduction of damage was achieved by immediate postinjury treatment with a combination of the following: an antiinflammatory substance, indomethacin; a stimulator of cytokine secretion, bacterial lipopolysaccharide; and the parent steroid, from which all other steroids arise, pregnenolone. This treatment reduced histopathological changes, spared tissue from secondary injury, and increased restoration of motor function. Remarkably, 11 of 16 of the animals treated with the above combination were able to stand and walk at 21 days after injury, 4 of them almost normally. The results were far superior to those obtained in controls or in animals to which the substances were given separately or in combination of two. This approach may prove to be applicable to nervous system injury, in general, and to injury in other tissues.     

27例肾腺癌的图象细胞仪定量分析

测定２７例肾腺癌的ＤＮＡ含量，并联系肿瘤的分级等部分形态学指标，对透明细胞型和颗粒细胞型肿瘤进行比较分析。发现：透明细胞型肾腺癌的分化一般较好，ＤＮＡ以二倍体为主，预后相对较好；而颗粒细胞型肾腺癌的分化较差，ＤＮＡ以非二倍体为主，其预后较差。