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31.
高频电刀对家兔活体肌肉组织的作用 总被引:1,自引:0,他引:1
目的 :探讨高频电刀对肌肉组织结构的作用。方法 :用高频电极在不同输出功率时测定大白家兔活体肌肉组织的电流强度 ,灼伤中心区汽化深度和汽化表面积 ,光镜和电镜下观察创面下 0~ 6mm深度的组织结构变化。结果 :随着电流输出功率的增强 ,肌肉组织的电流强度 ,中心区汽化深度和汽化表面积也随之增加 ,创面深部组织显微和超微结构发生了热凝固性改变 ,损伤程度随着离中心区距离的延长而减弱 ,但在 6mm深处仍有较明显的损伤性改变。结论 :用高频电刀操作时应注意对深部肌肉组织的损伤性作用 ,为减少损伤应注意操作时的电流强度和电灼时间。 相似文献
32.
急性鸭乙型肝炎病毒感染病毒清除机理研究 总被引:2,自引:0,他引:2
目的 :进一步阐明嗜肝病毒自然感染过程中病毒清除机理。方法 :7只 2~ 3月龄成年重庆麻鸭静脉接种 10~ 2 0mlDHBV阳性血清 (5× 10 8~ 1× 10 9genome) ,接种后每周采血检测外周血中DHBVDNA、DHBsAg和特异抗体的产生 ;感染后第10、35天分离外周血单个核细胞用于抗原特异细胞增殖实验 ;第 5、30、6 0天取肝组织标本进行DHBVDNASouthern杂交、DHB sAg免疫组化及肝组织病理检测。结果 :DHBV感染成年鸭在 1~ 2w潜伏期后出现急性、一过性感染 ,感染高峰期肝内存在多拷贝的DHBV所有复制中间体形式 ,包括cccDNA。进一步分析显示病毒血症的消失是在快速抗原特异细胞增殖反应和高滴度特异抗体产生之后 ;与此同时 ,整个急性感染期间 ,肝细胞并无明显的损害。结论 :非细胞直接损伤机制在嗜肝病毒清除过程中发挥了重要作用。 相似文献
33.
Polymorphisms and the differential antiviral activity of the chicken Mx gene 总被引:29,自引:0,他引:29 下载免费PDF全文
Ko JH Jin HK Asano A Takada A Ninomiya A Kida H Hokiyama H Ohara M Tsuzuki M Nishibori M Mizutani M Watanabe T 《Genome research》2002,12(4):595-601
The nucleotide sequence of chicken Mx cDNA was reported earlier using the White Leghorn breed in Germany, but it showed no enhanced resistance to viruses. In this study, the nucleotide sequences of chicken Mx cDNA were determined in many breeds. A total of 25 nucleotide substitutions, of which 14 were deduced to cause amino acid exchanges, were detected, suggesting that the chicken Mx gene is very polymorphic. Transfected cell clones expressing chicken Mx mRNA were established after the Mx cDNA was constructed with an expression vector and introduced into mouse 3T3 cells, and the Mx genes from some breeds were demonstrated to confer positive antiviral responses to influenza virus and vesicular stomatitis virus. On the basis of the comparison among the antiviral activities associated with many Mx variations, a specific amino acid substitution at position 631 (Ser to Asn) was considered to determine the antivirally positive or negative Mx gene. Thus, a single amino acid substitution influences the antiviral activity of Mx in domesticated chickens. 相似文献
34.
Suzuki J Kawasaki Y Nozawa R Isome M Suzuki S Takahashi A Suzuki H 《Asian Pacific journal of allergy and immunology / launched by the Allergy and Immunology Society of Thailand》2003,21(3):193-197
We present a case report of a 10 years old boy with protein-losing enteropathy and eosinophilic gastroenteritis who had positive histamine release tests, increased allergen-specific IgE antibodies to some food items, and low levels of total serum protein and albumin. Upper gastrointestinal endoscopy revealed a number of polyps and diffuse gastritis. Biopsy specimens of the stomach and duodenum showed widespread eosinophilia and neutrophilia. Although a restricted diet was recommended, a diet which excluded foods with positive results to both histamine release test and allergen-specific IgE antibodies was poorly tolerated, and the patient rejected systemic administration of corticosteroids. Thus, we initiated an oral disodium cromoglycate (DSCG) and ketotifen therapy. After oral DSCG and ketotifen administration, the patient's condition improved gradually. Therefore, oral DSCG and ketotifen therapy might be considered as treatment option in patients with eosinophilic gastroenteritis and protein-losing enteropathy caused by food allergy. 相似文献
35.
Retrograde dopaminergic neuron degeneration following intrastriatal proteasome inhibition 总被引:8,自引:0,他引:8
Recent studies have suggested that defects in the ubiquitin-proteasome system (UPS) contribute to the etiopathogenetic mechanisms underlying dopaminergic neuronal degeneration in Parkinson's disease. The present study aims to study the effects of proteasome inhibition in the nerve terminals of nigrostriatal dopaminergic neurons in the substantia nigra pars compacta (SNpc). Following a unilaterally intrastriatal injection of lactacystin, a selective proteasome inhibitor, dopaminergic neurons in the ipsilateral SNpc progressively degenerated with alpha-synuclein-immunopositive intracytoplasmic inclusions. When lactacystin was administered at a high concentration, the striatum was simultaneously involved, and alpha-synuclein-immunopositive extracytoplasmic granules appeared extensively within the SN pars reticulata (SNpr). In addition, during the retrograde neuron degeneration in SN, the level of heme oxygenase-1 immunopositivity, an oxidative stress marker, was markedly increased in SNpc neurons. These results reveal that intrastriatal proteasome inhibition sufficiently induces retrograde dopaminergic neuronal degeneration with abundant accumulation of alpha-synuclein in the SN. 相似文献
36.
Immunohistochemical localization of 3β-hydroxysteroid dehydrogenase/Δ5→4-isomerase (3β-HSD), which converts Δ5-3β-hydroxysteroids to Δ4-3-ketosteroids, was performed in the human adrenal gland and in its disorders by employing a specific antibody raised against
the enzyme purified from human placenta. Immunoreactivity of 3β-HSD was present in all three cortical zones of the adrenal
glands obtained at autopsy, while in surgically removed adrenal glands, immunoreactivity was dominant in the zona fasciculata
(ZF), with faint immunoreactivity in the zona glomerulosa (ZG) and the zona reticularis (ZR). Intracortical localization of
3β-HSD in the adrenal glands obtained at autopsy may represent an adrenal adaptation to antemortem stress, with shifting of
adrenal pregnenolone- a substrate of 3β-HSD - as well as steroid 17α-hydroxylase from adrenal androgen synthesis to glucocorticoid
synthesis. In adrenocortical hyperplasia, marked immunoreactivity was observed in the ZG and outer ZF in adrenal glands with
idiopathic hyperaldosteronism and in the ZF and ZR, especially in cortical micronodules, in the adrenal glands associated
with Cushing’s disease. In aldosteronoma and Cushing’s adenoma, immunoreactivity of the enzyme was much more intense in large
clear tumor cells than in small compact tumor cells. Immunolocalization of 3β-HSD can yield important information toward an
understanding of adrenal steroid metabolism in both physiological and pathological processes. 相似文献
37.
Babak S. Jahromi Yasuo Aihara Jinglu Ai Zhen-Du Zhang George Weyer Elena Nikitina Reza Yassari Khaled M. Houamed R. Loch Macdonald 《Neuroscience letters》2008
The pathogenesis of cerebral vasospasm after subarachnoid haemorrhage (SAH) involves sustained contraction of arterial smooth muscle cells that is maximal 6–8 days after SAH. We reported that function of voltage-gated K+ (KV) channels was significantly decreased during vasospasm 7 days after SAH in dogs. Since arterial constriction is regulated by membrane potential that in turn is determined predominately by K+ conductance, the compromised K+ channel dysfunction may cause vasospasm. Additional support for this hypothesis would be demonstration that K+ channel dysfunction is temporally coincident with vasospasm. To test this hypothesis, SAH was created using the double haemorrhage model in dogs and smooth muscle cells from the basilar artery, which develops vasospasm, were isolated 4 days (early vasospasm), 7 days (during vasospasm) and 21 days (after vasospasm) after SAH and studied using patch-clamp electrophysiology. We investigated the two main K+ channels (KV and large-conductance voltage/Ca2+-activated (KCa) channels). Electrophysiologic function of KCa channels was preserved at all times after SAH. In contrast, function of KV channels was significantly decreased at all times after SAH. The decrease in cell size and degree of KV channel dysfunction was maximal 7 days after SAH. The results suggest that KV channel dysfunction either only partially contributes to vasospasm after SAH or that compensatory mechanisms develop that lead to resolution of vasospasm before KV channels recover their function. 相似文献
38.
A mouse model of galactose-induced cataracts 总被引:4,自引:0,他引:4
Ai Y Zheng Z O'Brien-Jenkins A Bernard DJ Wynshaw-Boris T Ning C Reynolds R Segal S Huang K Stambolian D 《Human molecular genetics》2000,9(12):1821-1827
Galactokinase (GK; EC 2.7.1.6) is the first enzyme in the metabolism of galactose. In humans, GK deficiency results in congenital cataracts due to an accumulation of galactitol within the lens. In an attempt to make a galactosemic animal model, we cloned the mouse GK gene (Glk1) and disrupted it by gene targeting. As expected, galactose was very poorly metabolized in GK-deficient mice. In addition, both galactose and galactitol accumulated in tissues of GK-deficient mice. Surprisingly, the GK-deficient animals did not form cataracts even when fed a high galactose diet. However, the introduction of a human aldose reductase transgene into a GK-deficient background resulted in cataract formation within the first postnatal day. This mouse represents the first mouse model for congenital galactosemic cataract. 相似文献
39.
Myotonic dystrophy, a progressive autosomal dominant disorder, is associated with an expansion of a CTG repeat tract located in the 3'-untranslated region of a serine/threonine protein kinase, DMPK. DMPK modulates skeletal muscle Na channels in vitro, and thus we hypothesized that mice deficient in DMPK would have altered muscle Na channel gating. We measured macroscopic and single channel Na currents from cell-attached patches of skeletal myocytes from mice heterozygous (DMPK(+/-)) and homozygous (DMPK(-/-)) for DMPK loss. In DMPK(-/-) myocytes, Na current amplitude was reduced because of reduced channel number. Single channel recordings revealed Na channel reopenings, similar to the gating abnormality of human myotonic muscular dystrophy (DM), which resulted in a plateau of Na current. The gating abnormality deteriorated with increasing age. In DMPK(+/-) muscle there was reduced Na current amplitude and increased Na channel reopenings identical to those in DMPK(-/-) muscle. Thus, these mouse models of complete and partial DMPK deficiency reproduce the Na channel abnormality of the human disease, providing direct evidence that DMPK deficiency underlies the Na channel abnormality in DM. 相似文献
40.
碱性成纤维细胞生长因子与卵巢癌的关系 总被引:3,自引:0,他引:3
目的 探讨碱性成纤维细胞生长因子 (basic fibroblast growth factor,b FGF)对卵巢癌细胞增殖、浸润和肿瘤血管生成的影响 ,及 b FGF单克隆抗体 (b FGF monoclonal antibody,b FGF- MAb)的治疗作用。 方法 将人卵巢癌细胞株 SKOV3接种于 2 4孔板 ,加入不同浓度的 b FGF,每日行结晶紫染色后测定光密度 (D4 90 )值 ,绘制细胞生长曲线 ;将 SKOV3细胞团接种于铺设有细胞外基质凝胶的 4孔板 ,每日测定癌细胞在凝胶中的浸润距离 ;建立 SKOV3细胞裸鼠皮下移植瘤模型 ,每周两次分别将 b FGF、b FGF-MAb和生理盐水注射于移植瘤周围 ,8周后测量肿瘤体积 ;对移植瘤组织切片行 因子的免疫组化染色、测定肿瘤内微血管密度 (microvessel density,MVD)。 结果 b FGF能促进 SKOV3细胞增殖并呈浓度依赖 ,实验第 5天 ,5 ng/ml、10 ng/ml组细胞 D4 90 值是对照组的 1.0 9倍和 1.2 1倍 ;b FGF能促进 SKOV3细胞浸润并呈浓度依赖 (P<0 .0 5 ) ,第 7天 ,5 ng/ml、10 ng/ml组细胞浸润距离分别是对照组的 1.5 3倍和2 .4 5倍 ;b FGF组移植瘤体积和 MVD分别是对照组的 1.80倍和 1.4 6倍 (P<0 .0 5 ) ,b FGF- MAb组移植瘤体积和 MVD分别是对照组的 6 3.7%和 6 2 .8% (P<0 .0 5 )。 结论 b FGF能明显促进卵巢癌细胞的增殖、 相似文献