Effect of kanamycin on the cochleas in guinea pigs

Kanamycin (400 mg/kg) was injected daily for 5 days in guinea pigs, the changes in CM and AP responses were recorded. The CM threshold was elevated in 34.5% of the animals and the CM amplitude was reduced when compared with normal animals (P less than 0.005). The AP threshold was elevated in 13.8%. The latency of N1 was increased (P less than 0.001) and the amplitude of N1 reduced at 80 dB (S-L) (P less than 0.001).cAMP and cGMP levels in the organ of Corti and stria vascularis spiral ligament decreased, and the decrease correlated with the changes in auditory function. We consider the reduction of cyclic nucleotides in the cochlea may be important in kanamycin ototoxicity.     

备劳特联合爱喘乐雾化吸入治疗儿童哮喘急性发作的疗效观察

目的 :观察氧雾化吸入备劳特和爱喘乐治疗儿童哮喘急性发作的疗效。方法 :选择 15 8例哮喘急性发作患儿作为研究对象 ,分为观察组和对照组。观察组采用氧驱动雾化吸入备劳特和爱喘乐 ;对照组静脉滴注氨茶碱和地塞米松。结果 :观察组显效率 87.3% (96 /110 ) ,总有效率 99.1% (10 9/110 ) ,临床治愈率 94 .5 % (10 4 /110 ) ;对照组显效率 2 2 .9% (11/48) ,总有效率 6 0 .4 % (2 9/48) ,临床治愈率 5 4 .2 % (2 6 /48)。观察组显效率、总有效率及临床治愈率皆显著高于对照组 ,差异有高度统计意义 (P <0 .0 1)。观察组治疗后心率较治疗前明显下降 ,差异有统计意义(P <0 .0 5 )。结论 :采用氧驱动雾化吸入备劳特和爱喘乐能快速、有效地控制哮喘发作。     

Freiberg’s Infraction in Identical Twins: A Case Report

Freiberg's infraction is an ostechondrosis of a lesser metatarsal head resulting in degeneration of the metatarsophalangeal joint. Several mechanisms have been suggested in its pathenogenesis. Freiberg first described the entity and believed single impact trauma was the underlying cause. Repetitive biomechanical microtrauma is the most widely accepted etiologic theory. Other factors contributing to its development include aseptic necrosis, ischemia, and a congenital predisposition. We present a case report of Freiberg's infraction occurring in identical twins involving multiple metatarsals in various stages of degeneration. One of the twins was affected unilaterally whereas the other twin was affected bilaterally. Both twins had involvement of the second metatarsal on the same side extremity. The occurrence of Freiberg's infraction in identical twins suggests that an underlying congenital predisposition to the condition may play more of a role than previously considered.     

神经化组织工程骨构建的初步观察

目的评估两种组织工程骨体内神经化重建方法的成骨效果,研究神经化与成骨的相互关系。方法26只新西兰大白兔,其中24只随机分成四组:组织工程骨组(A组),感觉神经束植入组(B组),运动神经束植入组(C组),血管束植入组(D组);另2只为空白对照组。每只动物均制备左侧股骨长1.5cm的段缺性骨与骨膜缺损,钢板固定后骨缺损处分别植入用四种方法制备的组织工程骨。植入的神经分别是隐神经和股神经肌支。术后4、8、12周摄股骨正位X线片,用放射影像学评分和X线阻射影分析比较骨缺损修复情况。结果在组织工程骨中植入感觉神经束后,比单纯组织工程骨和运动神经束植入的修复效果均有明显提高,而在组织工程骨中植入运动神经束与单纯组织工程骨修复骨缺损的效果相比较无明显差异,感觉神经束植入与血管束植入的成骨效果比较无明显差异,血管束植入组的成骨效果优于其它两组。结论利用感觉神经束植入的方法可以提高组织工程骨的成骨作用,而植入运动神经束却无此作用。     

Effect of nuclear factor-κB p65 ASOND on I typ collagen expression and rat hepatic stellate cells proliferation

Objective Sstudy effect of nuclear factor-κB ASOND on I type collagen expression and rat hepatic stellate cells(HSC)proliferation.Methods Rat HSCs were separated by affusing and digestingof Ⅳ type collagenenzyme and density acentric method.Lipid-mediated NF-κB p65 ASOND(0.001,0.01,0.1,1μmol/L)Was transferred into rat HSCs.Toxicity of HSCs caused by NF-κB p65 ASOND and activity of LDH were determined by trypan blue staining.Proliferation affection of transferring NF-κB p65 ASOND into HSCs was determined by MTT.In different concentration NF-κB p65 ASOND.expression of Ⅰ type collagen stimulated by 1mg/L TNF-αwas determined by RT-PCR and ELISA.Results After transfection of NF-κB p65 ASODN,expression of NF-κB protein in HSCs was decreasing.Toxicity experiment indicated that NF-κB p65 ASOND of different concentration(0.001,0.01,0.1 and 1.0 μmol/L)had no effect on HSCs livability and LDH activity(P<0.05).Four different concentration NF-κ B p65 ASOND could restrain HSCs proliferation stimulated by 1 mg/L TNF-α.The expression of I type collagen and mRNA stimulated by 1mg/LTNF-αwas increased,and had a positive correlation with concentration(P>0.05).Conclusion NF-κB p65 ASOND may depress NF-κB activity to restrain HSCs proliferation and Ⅰ type collagen expression,and reduce extracellular matrix.     

使用抗体-生物纳米磁珠复合体的免疫凝集检测法

使用从磁性细菌体内提取的生物纳米磁珠，在磁珠表面联上特定的抗体，以玻片免疫凝集检测法检测癌胚抗原（carcinoembryonic AntigenCEA）。结果表明使用5μg的CEA抗体-磁珠复合体，可以检测出100pg／ml浓度的CEA，在操作性、经济性上优于免疫荧光检测法。     

半导体激光穴位照射与药物治疗急慢性关节损伤的疗效比较

目的探讨低功率半导体激光对急慢性关节损伤的治疗作用.方法1999年至2004年急慢性关节损伤的患者240例,随机分为两组,各120例,激光组采用低功率半导体激光治疗仪照射痛点;对照组口服活血止痛胶囊,外用沈阳红药膏治疗.结果两周后的疗效比较.激光组有效率为85.0%,痊愈率为44.2%;对照组有效率为71.7%,痊愈率为20.8%;差异有显著意义.激光组中,急性关节损伤的患者痊愈率为52.9%,慢性关节损伤患者的痊愈率为32.7%,差异有显著意义(P＜0.05)有效率,差异无显著性意义.结论低功率半导体激光对急慢性关节损伤,尤其是急性小关节损伤有很好的疗效.     

Crohn病的CT表现

目的探讨Crohn病的CT表现特点。方法回顾性分析经病理证实的18例Crohn病的CT征象，大多数病例采用螺旋CT或多层螺旋CT扫描。结果所有病例均有节段性肠壁增厚，CT增强有7例表现为肠壁均匀增厚，缺乏层次，同时肠壁强化不明显；有11例表现为黏膜增厚和分层形成“靶征”和“双环征”，肠壁厚度多在5～10mm；有9例表现为病变段肠管周围纤维脂肪成分增多，蜂窝织炎及肠周或肠系膜血管增多增粗。有5例CT首先考虑为其他疾病，1例考虑为闭攀性肠梗阻，1例首先考虑为肠结核，2例回盲部病变怀疑为癌，1例肠穿孔形成脓肿仅诊为化脓性感染。结论cT不但能准确显示Crohn病肠壁、肠系膜及周围结构的异常，增强CT还能区分活动性与非活动性病变，有助于临床采取合理而积极的治疗方案。