抗龋IgY和IgY牙膏对体外培养细胞影响的形态学观察

目的：了解抗龋IgY和IgY牙膏对体外培养细胞的影响。方法：体外培养人成纤维细胞（human skin fibrobasts,HSF）和中国仓鼠卵巢癌细胞（chinese hamster ovary,CHO），分别经不同浓度的抗龋IgY和IgY牙膏处理后常规培养，通过倒置显微镜和电子显微镜观察细胞生长情况和细胞超微结构。结果：经抗龋IgY处理的HSF和CHO细胞生长旺盛，超微结构无变化；经抗龋IgY牙膏处理的HSF和CHO细胞加药后部分细胞出现变圆或脱落、细胞生长缓慢和细胞器变性等变化，第2天恢复良好生长状态。结论：抗龋IgY对体外培养细胞生长、形态无影响；抗龋IgY牙膏中的某些成分可能对培养细胞有短暂的不良影响。     

重视患者知情同意权提高医疗服务质量

为了提高医院的医疗服务质量，保证医疗安全，避免和减少医疗纠纷，医院必须严格遵守患者知情同意原则。作者着重讨论知情同意的意义，如何规范知情同意书的范围、格式和内容，以及填写注意事项等问题。     

自动平衡记录仪与HPLC匹配定量测定腺苷酸

本文以腺苷酸为测定物,探讨了以国产自动平衡记录仪为高效液相色谱记录并定量分析结果的方法。结果表明,从自动平衡记录上得到峰形、计算结果均与原机所带积分仪的一致。其稳定性、灵敏度和线性响应均能达到高效液相色谱检测器的要求,是一种可以代替积分仪分析结果的经济、可靠的方法。     

ARRIF-Ⅲ型的研制、生物力学测试及临床初步应用

目的　研制一种新型腰椎滑脱复位内固定器 ,在此内固定器固定后 ,能方便地平行置入两枚BAK ;同时测试RF Ⅱ型及ARRIF Ⅲ型的生物力学特点。方法　使用对照法 ,10具健康成年人腰2 ～骶2 椎体标本 ,制成腰椎滑脱模型 ,用生物力学测试机测试RF Ⅱ型及ARRIF Ⅲ型固定后的脊柱标本复合物的应力遮挡及应力 应变。结果　 30 0 0N轴向载荷下及 2 0Nm前屈与侧弯力矩下RF Ⅱ型和ARRIF Ⅲ型的应力遮挡及应力 应变均有显著差异 ,但其绝对值较小。ARRIF Ⅲ型的临床初步应用取得了满意的效果。结论　两种内固定器的固定效果均较好 ,但RF Ⅱ型在固定后的即刻 ,有较显著的微动 ;ARRIF Ⅲ型在复位固定后可较方便地同时置入两枚BAK。ARRIF Ⅲ型可用于Ⅰ°～Ⅲ°的椎体滑脱症的复位与固定     

检测各型乙肝患者血小板功能的临床意义

检测198例各型乙肝患者血小板功能的五个项目：血小板总数、粘附试验、聚集试验、血块退缩、血小板第3因子有效性，发现各期乙肝患者血小板功能的异常有显著性差异（P＜0．01）．并提示乙肝患者除有血小板数量的改变外，还有质量的改变，因此，全面的血小板功能检测可作为估计乙肝患者肝损害程度的辅助指标．     

Fluorescence behaviour of human arterial tissue

Total fluorescence from arterial tissue is influenced by three factors: the absorption coefficient of tissue at a specific excitation wavelength, the laser excitation power and the fluorescence coefficient which is related to chemical species in tissue. These various influences were demonstrated by the following experimental results in vitro: (1) the effect of increasing power on fluorescence intensity, (2) the total fluorescence intensity in normal aorta and plaque and (3) the effect of a chromophore such as β-carotene on total fluorescence intensity. The fluorescence intensity of normal artery is an incremental function of laser excitation power, and the fluorescence emission from normal artery compared to fluorescence emission from plaque is significantly different at the same excitation power. The total fluorescence of normal artery was measured to be twice as great as that of atheromatous plaque (relative mean ratio of 2.58±0.46 compared to unity,p<0.0002 at 488 nm; relative mean ratio of 2.57±0.51 compared to unity,p<0.0009 at 514 nm). The total fluorescence emission decreases with the increase of β-carotene content in arterial tissue (R=0.97). These emission differences, when intensified by an exogenous chromophore of β-carotene, may provide an improved guidance signal for diagnosis of plaque from normal artery during laser angioplasty procedures.     

Comparative Study on the Immunogenicity between Recombinant MS-Sj26GST Vaccine and Recombinant BCG-Sj26GST Vaccine in Schistosoma japonicum

TuberculosisandSchistosomiasisarethemajorcontagiousdiseaseswhicharethemostdangeroustothepeople’shealth Inordertogetridofthem ,wemustlookforamoreusefulvaccine Bythetech niquesofmolecularbiology ,2 6 0 0 0DaGlutathionStransferase (GST) genewasclonedintotheE coli MycobacteriumtransferringandexpressionvectorpBCG 2 0 0 0totransformittoMycobacteriumsmeg matismc2 15 5 (MS)andBCGseparatelyinordertoconstructrMS Sj2 6GSTvaccineandrBCG Sj2 6GSTvaccine Inthisstudy ,theBALB/cmicewereimmu niz…     

一种中西药复方制剂(PESM)对大鼠实验性肾结石形成的抑制作用

用乙二醇法诱导大鼠产生草酸钙结石，用45~Ca示踪测定法了解中草药复方制剂（PESM）对草酸钙结石形成的抑制作用。结果显示，实验组肾脏放射性强度明显减少（实验组3.47±3.52cpm／mg，对照组10.89±14.24cpm／mg）。干肾重量明显下降（实验组306.0±47.0mg，对照组343.1±59.4mg），说明PESM能明显抑制肾脏草酸钙结石的形成。     

A study on various symptoms of chronic hepatitis treated with radix astragali and its regulative effect to levels of serum hormone

Sixty-seven cases of chronic hepatitis were treated with Radix Astragali. After treatment (2-month course), the clinical improvement rate in 38 cases of the Stagnation of the Liver-Qi and Deficiency of the Spleen type was 92.1%, and in 26 cases of the Deficiency of Liver Yin and Kidney Yin type was 88.5%, more effective than in the control group (P<0.05). The regulative effect to the levels of serum hormone was observed in the patients of the Stagnation of the Liver-Qi and Deficiency of the Spleen type treated with this medicine. The results showed that the levels of serum triiodothyronine, estradiol (female) and testosterone (male) were increased after treatment (1.40±1.38 ng/dl, 129.30±1.23 pg/ml and 496.24±1.47 ng/dl). Pre-treatment levels were 1.22±1.49 ng/dl, 104.60±1.45 pg/ml and 398. 17±1.55 ng/dl respectively (P<0.05); however, the level of serum prolactin (2.75±4.46 ng/ml) was lower after treatment than before treatment (3.20±3.82 ng/ml,P<0.05). No obvious changes were observed in the levels of serum follicle stimulating hormone, luteinizing hormone, thyroid stimulating hormone, thyroxine, triiodothyronine uptake ratio and cortisol after treatment.