MiR-19a mediates the negative regulation of the NF-κB pathway in lipopolysaccharide-induced endometritis by targeting TBK1

Objective

In both humans and animals, endometritis is severe inflammation of the uterus, and it causes great economic losses in dairy cow production. MicroRNAs have been reported to play an important role in various inflammatory diseases. However, the regulatory mechanisms of miR-19a in endometritis remain unclear. Thus, the aims of this study are to investigate the role of miR-19a in a mouse model of lipopolysaccharide (LPS)-induced endometritis and elucidate the possible mechanisms in bovine endometrial epithelial cells (bEECs).

Methods and results

Histological analysis showed that LPS induced severe pathological changes, suggesting that the endometritis mouse model was well established. The qPCR assay indicated that miR-19a expression in the uterine tissues of mice with endometritis and in bEECs with LPS stimulation was significantly reduced. The overexpression of miR-19a significantly decreased the expression of inflammatory cytokines (TNF-α, IL-6 and IL-1β) and the phosphorylation of NF-κB p65 and IκBα. Similar results were also obtained following the knockdown of TBK1. Furthermore, a dual luciferase reporter assay further validated that miR-19a inhibited TBK1 expression by binding directly to the 3′-UTR of TBK1.

Conclusion

We demonstrated that miR-19a has anti-inflammatory effects and mediates the negative regulation of the NF-κB Pathway in LPS-induced endometritis by targeting TBK1.

     

Adoptive transfer of IFN-γ-induced M-MDSCs promotes immune tolerance to allografts through iNOS pathway

Inflammation Research - Efficient production of monocytic myeloid-derived suppressor cells (M-MDSCs) with stable immunosuppressive function is crucial for immunomodulatory cell therapy for many...     

TNF-α/calreticulin dual signaling induced NLRP3 inflammasome activation associated with HuR nucleocytoplasmic shuttling in rheumatoid arthritis

Inflammation Research - The present study was undertaken to validate whether TNF-α and calreticulin (CRT) serve as dual signaling to activate nucleotide-binding oligomerization domain-,...     

Study on self-consciousness of children with learning disabilities and related factors

To study the self-consciousness of children with learning disabilities (LD) and to identify related factors. Methods Five hundred and sixty pupils graded from 1 to 6 in an elementary school were investigated. According to the pupil rating scale revised screening for learning disabilities (PRS), combined Raven‘s test (CRT) and achievement of main courses, 35 of 560 pupils were diagnosed as LD children. Thirty-five children were selected from the average children and 35 from advanced children in academic achievement equally matched in class, gender, and age with LD children as control groups. The three groups were tested by Piers-Harris children‘s self-concept scale. Basic information of each subject was collected by self-made questionnaire. Results Compared with the average and advanced children, LD children got significantly lower scores in self-concept scale. Based on logistic regression analysis, 3 factors were identified, including family income per month, single child and delivery model. Conclusion The results suggest that self-consciousness of children with LD is lower than that of normal children.     

Peripheral blood leukocyte telomere length is associated with age but not renal function: A cross-sectional follow-up study

Objectives

We aimed to evaluate the relationship between baseline renal function and changes in telomere length in Han Chinese.

Methods

The telomere restriction fragment (TRF) length of leukocytes in the peripheral blood was measured in healthy volunteers recruited in 2014. The estimated glomerular filtration rate (eGFR) was calculated based on serum creatinine (Scr) and serum cystatin C (CysC)-eGFRcys and eGFRScr-cys through the Cockcroft-Gault formula (eGFRC-G) or the Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI / eGFRCKD-EPI) equation. The correlation between telomere length changes over time and renal function was analyzed.

Results

Leukocyte TRF lengths were negatively correlated to age (r = -0.393, p < 0.001) and serum CysC (r = -0.180, p < 0.01), while positively associated with eGFRCKD-EPI, eGFRC-G, eGFRcys, and eGFRScr-cys (r = 0.182, 0.122, 0.290, and 0.254 respectively, p < 0.01). The 3-year change of telomere length was 46 bp/years. When adjusted for age, the associations between telomere length changes and baseline, subsequent TRF lengths, and serum CysC were no longer present. No association was observed between TRF length changes and renal function.

Conclusion

The rate of telomere length changes was affected by age and baseline telomere length. The telomere length changes might be important markers for aging.

     

DF暂封糊剂的研制

目的：研制一种单组份、糊剂型、水硬性暂封材料。方法：通过测定材料在水中的凝固速度及凝固后材料表面性状，对水硬性材料成份的种类、赋形剂的种类及凝固促进剂的种类进行筛选。结果：水硬性材料Ａ２的凝固速度最快，赋形剂Ｂ２的效果最好，凝固促进剂Ｃ１的作用优于Ｃ２。结论：采用Ａ２、Ｂ２、Ｃ１作为暂封材料基本组成效果最佳，凝固２４ｈ后抗压强度达１３．９４ＭＰａ±２．０７ＭＰａ。     

Root canal systems in mandibular first premolars with C-shaped root configurations. Part I: Microcomputed tomography mapping of the radicular groove and associated root canal cross-sections

The purpose of this study was to investigate the morphology of the radicular grooves and root canal cross-sections in mandibular first premolars that exhibit a C-shaped root. Eighty-six mandibular first premolars with C-shaped roots were scanned from the coronal to anatomical apex using microCT80. The location, length, and depth of the radicular grooves were measured. The cross-sectional morphology of root canal systems was evaluated at 11 levels. Results were subject to the Kruskal-Wallis test. Of the 86 C-shaped roots, a total of 93 radicular grooves were noted. Most of the grooves (78.5%) were located on the mesiolingual surface of the root. The C-shaped canal could vary considerably in shape at different levels, and the incidence of two canals at the middle and apical was 21% and 80%, respectively. This study shows that radicular grooves may present relevant morphologic variations in the root canal system when the C-shaped root is present.     

Anthropometric study of the upper lip and the nose of infants less than a year of age

We divided healthy newborns (aged between 2 weeks and 6 months) into four groups, less than 2 weeks old, 60 +/- 7 days, 120 +/- 7 days, and 180 +/- 7 days, between June 2001 and February 2002, and each group had 40 infants. The lineal distances included 13 items related to the nose, mouth, and lips.The average width of the columella at the midpoint was 3.2, 3.5, 3.7, and 3.8 mm for the 2-week-old group, the 2-month-old group, the 4-month-old group, and the 6-month-old group, respectively. The average height of the columella was 4.7, 4.9, 5.2, and 5.3 mm. The average length between the medial alar bases was 13.7, 14.4, 17.4, and 17.6 mm. The average length from the base to the tip of Cupid's bow was 9.5, 10.0, 10.5, and 10.6 mm. The average length from the columella lateral base to the tip of Cupid's bow was 8.4, 9.9, 10.2, and 10.5 mm. The average length from the columella central base to the center of Cupid's bow was 8.3, 9.5, 9.8, and 9.9 mm. The average width of one limb of Cupid's bow was 2.7, 3.1, 3.4, and 3.5 mm. The average length from the tip of Cupid's bow to the commissure was 13.4, 14.7, 16.4, and 16.9 mm. The average intercommissural distance was 26.8, 30.3, 30.8, and 32.7 mm. The average width of the philtral column at the columella base was 3.1, 3.6, 3.7, and 4.0 mm. The average width of the philtral columns at the mid-portion was 3.7, 4.6, 4.6, and 4.6 mm. The average height of the nasal tip protrusion was 8.7, 11.0, 11.7, and 12.1 mm. The average width of the nose was 20.7, 23.7, 25.3, and 25.9 mm. In conclusion, these data are expected to be useful for patients with a bilateral cleft lip.