全文获取类型
收费全文 | 4064篇 |
免费 | 311篇 |
国内免费 | 42篇 |
专业分类
耳鼻咽喉 | 18篇 |
儿科学 | 178篇 |
妇产科学 | 35篇 |
基础医学 | 562篇 |
口腔科学 | 159篇 |
临床医学 | 362篇 |
内科学 | 847篇 |
皮肤病学 | 85篇 |
神经病学 | 255篇 |
特种医学 | 234篇 |
外科学 | 539篇 |
综合类 | 55篇 |
预防医学 | 194篇 |
眼科学 | 48篇 |
药学 | 428篇 |
中国医学 | 13篇 |
肿瘤学 | 405篇 |
出版年
2022年 | 32篇 |
2021年 | 50篇 |
2020年 | 33篇 |
2018年 | 72篇 |
2017年 | 73篇 |
2016年 | 58篇 |
2015年 | 80篇 |
2014年 | 112篇 |
2013年 | 137篇 |
2012年 | 121篇 |
2011年 | 102篇 |
2010年 | 108篇 |
2009年 | 134篇 |
2008年 | 99篇 |
2007年 | 133篇 |
2006年 | 121篇 |
2005年 | 105篇 |
2004年 | 134篇 |
2003年 | 94篇 |
2002年 | 121篇 |
2001年 | 114篇 |
2000年 | 102篇 |
1999年 | 142篇 |
1998年 | 143篇 |
1997年 | 123篇 |
1996年 | 127篇 |
1995年 | 86篇 |
1994年 | 95篇 |
1993年 | 58篇 |
1992年 | 90篇 |
1991年 | 107篇 |
1990年 | 100篇 |
1989年 | 101篇 |
1988年 | 110篇 |
1987年 | 86篇 |
1986年 | 95篇 |
1985年 | 84篇 |
1984年 | 46篇 |
1983年 | 60篇 |
1982年 | 44篇 |
1981年 | 56篇 |
1980年 | 56篇 |
1979年 | 60篇 |
1978年 | 42篇 |
1977年 | 36篇 |
1976年 | 36篇 |
1975年 | 37篇 |
1974年 | 38篇 |
1973年 | 33篇 |
1971年 | 27篇 |
排序方式: 共有4417条查询结果,搜索用时 140 毫秒
121.
122.
Adipokines played a limited role in predicting temporary growth differences between very low birthweight infants with and without bronchopulmonary dysplasia
下载免费PDF全文
![点击此处可从《Acta paediatrica (Oslo, Norway : 1992)》网站下载免费的PDF全文](/ch/ext_images/free.gif)
123.
124.
Yang Liu Yoshifumi Baba Takatsugu Ishimoto Hiroyasu Tsutsuki Tianli Zhang Daichi Nomoto Kazuo Okadome Kensuke Yamamura Kazuto Harada Kojiro Eto Yukiharu Hiyoshi Masaaki Iwatsuki Yohei Nagai Shiro Iwagami Yuji Miyamoto Naoya Yoshida Yoshihiro Komohara Masaki Ohmuraya Xiaoming Wang Jaffer A. Ajani Tomohiro Sawa Hideo Baba 《British journal of cancer》2021,124(5):963
Background Fusobacterium nucleatum (F. nucleatum) is a gut microbe implicated in gastrointestinal tumorigenesis. Predicting the chemotherapeutic response is critical to developing personalised therapeutic strategies for oesophageal cancer patients. The present study investigated the relationship between F. nucleatum and chemotherapeutic resistance in oesophageal squamous cell carcinoma (ESCC).Methods We examined the relationship between F. nucleatum and chemotherapy response in 120 ESCC resected specimens and 30 pre-treatment biopsy specimens. In vitro studies using ESCC cell lines and co-culture assays further uncovered the mechanism underlying chemotherapeutic resistance.Results ESCC patients with F. nucleatum infection displayed lesser chemotherapeutic response. The infiltration and subsistence of F. nucleatum in the ESCC cells were observed by transmission electron microscopy and laser scanning confocal microscopy. We also observed that F. nucleatum modulates the endogenous LC3 and ATG7 expression, as well as autophagosome formation to induce chemoresistance against 5-FU, CDDP, and Docetaxel. ATG7 knockdown resulted in reversal of F. nucleatum-induced chemoresistance. In addition, immunohistochemical studies confirmed the correlation between F. nucleatum infection and ATG7 expression in 284 ESCC specimens.Conclusions F. nucleatum confers chemoresistance to ESCC cells by modulating autophagy. These findings suggest that targeting F. nucleatum, during chemotherapy, could result in variable therapeutic outcomes for ESCC patients.Subject terms: Tumour biomarkers, Oesophageal cancer 相似文献
125.
High calcium enhances the expression of double‐stranded RNA sensors and antiviral activity in epidermal keratinocytes
下载免费PDF全文
![点击此处可从《Experimental dermatology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Yuriko Yamamura Shin Morizane Takenobu Yamamoto Jun Wada Keiji Iwatsuki 《Experimental dermatology》2018,27(2):129-134
Double‐stranded RNA (dsRNA) sensors including TLR3, MDA5 and RIG‐I are expressed in epidermal keratinocytes and play an important immunological role by enhancing various innate and adaptive immune responses. Although the role of elevated extracellular calcium concentration in keratinocyte differentiation is well understood, the effect of high calcium on dsRNA sensors is not well studied. We investigated alterations in dsRNA sensor expression and antiviral activity induced by a high extracellular concentration of calcium in epidermal keratinocytes. Normal human epidermal keratinocytes (NHEKs) were stimulated with high calcium and/or synthetic dsRNA, poly (I:C). TLR3, IFIH1 (MDA5) and DDX58 (RIG‐I) expression were measured via qPCR, and IFN‐β and human beta‐defensin 2 (HBD2) levels were measured using ELISA. TLR3 localization was evaluated with immunocytofluorescence. Antiviral activity was quantified with virus plaque assays using herpes simplex virus type 1 (HSV‐1). High calcium significantly upregulated mRNA expression of TLR3, IFIH1 and DDX58 in NHEKs. In addition, high calcium significantly enhanced poly (I:C)‐induced anti‐HSV‐1 activity in NHEKs. The antiviral molecule HBD2 but not IFN‐β induction by poly (I:C) was enhanced by high calcium. Our findings indicate that high levels of extracellular calcium enhance the expression of dsRNA sensors and augment antiviral activity in epidermal keratinocytes. 相似文献
126.
127.
Graziani G; Pasqualetti D; Lopez M; D'Onofrio C; Testi AM; Mandelli F; Gallo RC; Bonmassar E 《Blood》1987,69(4):1175-1181
Peripheral mononuclear cells (MNC) collected from 12 healthy donors and 44 leukemic patients at various stages of the disease were tested for natural killer (NK) activity and for their susceptibility to HTLV-I infection in vitro, measured in terms of percentage of p19 positive cells. MNC from leukemic donors at any stage of leukemia (ie, onset or relapse, ON/REL; complete remission or off-therapy, CR/OT donors) were highly susceptible to HTLV-I infection. This was true for acute leukemias of lymphoblastic (ALL) or nonlymphoblastic (ANLL) type. MNC of ON/REL patients were more susceptible to HTLV-I than those of CR/OT donors. In addition, leukemic blasts were more rapidly infected (ie, within five to seven days) than the HTLV-I-susceptible normal cord- blood lymphocytes. However, the presence of circulating blasts was not essential to virus susceptibility, since CR/OT MNC, presumably free of leukemic blasts, were still more susceptible to HTLV-I than normal cells. Basal NK function of MNC from leukemic patients was significantly lower than that detectable in healthy controls. However, no correlation was found between susceptibility to HTLV-I infection and NK activity. 相似文献
128.
Burst patterns in the digastric, mylohyoid, and masseter muscles and the resultant jaw movement orbits during chewing and
swallowing were investigated in the freely behaving rabbit. Activities in the posterior mylohyoid fibers consisted of two
continuous bursts. Peaks in the first burst of the posterior fibers occurred in the middle part of opening and preceded the
digastric burst. Peaks in the second burst occurred in the final part of opening and coincided with those in the working side
of the digastric burst. After removal of the bilateral digastric muscles, the gape size during chewing was largely reduced
in the final part of opening and in the early part of closing. The results suggest that (a) the digastric may have a role
in opening the mandible widely beyond the rest position but may not have a major role in the control of the horizontal (mediolateral)
jaw movement, (b) the posterior mylohyoid fibers may have a function as an elevator of the tongue in the early part of opening,
and (c) the posterior mylohyoid fibers may have a function as a depressor of the jaw in the late part of opening. Electromyographic
burst in the mylohyoid muscle began with marked activity in the mid-closing phase. The results support a role for the mylohyoid
muscle as a leading muscle of swallowing. Swallowing events in the rabbit are easily distinguished from the activities of
the mylohyoid muscle and the thyrohyoid muscle. 相似文献
129.
Spontaneous echo contrast in the descending aorta (DA-SEC) was examined as a possible risk factor for cerebral thromboembolism. In 19 patients (10 males, 9 females) in the chronic stage of cerebral infarction, abnormal findings by transesophageal echocardiography, flow dynamics of the common carotid artery (CCA), and hemostatic factors including blood coagulation and fibrinolysis were investigated. In nine patients, DA-SEC was detected, and SEC in left atrium (LA-SEC) was detected in nine patients. The DA-SEC positive group showed decreased blood-flow velocity (BFV) in bilateral CCA, high levels of thrombin-antithrombin III complex (TAT) and prothrombin fragment 1.2 (F1+2), a decrease in platelet count and a slight increase in D-dimer, which means an activated state of thrombin generation and resulting fibrinolysis, compared to the DA-SEC negative group. On the other hand, the LA-SEC positive group showed normal BFV in CCA and only a slight increase in D-dimer. We conclude that the condition producing DA-SEC is a stronger risk factor for cerebral infarction than that producing LA-SEC. 相似文献
130.
Jacqueline AM Smith DL Patil OT Daniels Y-S Ding J-D Gallezot S Henry KHS Kim S Kshirsagar WJ Martin GP Obedencio E Stangeland PR Tsuruda W Williams RE Carson ST Patil 《The international journal of neuropsychopharmacology / official scientific journal of the Collegium Internationale Neuropsychopharmacologicum (CINP)》2015,18(2)