Coexistence of Low and High Grade Squamous Intraepithelial Lesions of the Cervix: Morphologic Progression or Multiple Papillomaviruses?

Background.The diagnosis of both low (LSIL) and high (HSIL) grade squamous intraepithelial lesions in the same cervical specimen may reflect classification variation, morphologic progressionin situ,and, conceivably, different HPV infections. We addressed these possibilities in cervical specimens previously diagnosed as containing both LSIL (condyloma/CIN1) and HSIL (CIN2/3).Methods.All cases with a histologic diagnosis of LSIL and HSIL from 1994–1996 were reviewed. On review, lesions were scored as (1) no significant variation in lesion grade (classification discrepancies) and showing a (2) one (CIN1–2) or (3) two (CIN1–3) grade shift in the same case. In cases in which a one or two grade shift was confirmed, low (CIN1) and high (CIN2–3) grade foci were microdissected and extracted DNA analyzed for HPV by PCR and RFLP analysis.Results.Of 98 cases available for review, 58 (59%) did not exhibit significant variation in grade (classification discrepancy), and 40 (41%) showed a one (25) or two (15) grade shift. Of the latter group both LSIL and HSIL foci were HPV(+) in 26 (65.0%). The same HPV was present in both LSIL and HSIL foci in 15/15 lesions with a one grade shift (CIN1–2). In contrast, a significantly higher proportion of lesions with a two grade shift (CIN1–3) contained two different HPV types (4/11 vs 0/15;P= 0.01). Combinations of HPVs in the low/high grade foci, respectively, included HPV 11/16 (1), 11/16 + 18 (1), and HPV39/16 (2).Conclusions.Lesions containing LSIL and HSIL which span two grades (CIN1 and CIN2) most likely represent morphologic progression in a single infection. Lesions containing CIN1 and CIN 3 may be attributed to both lesion progression and two coincident infections; the latter sometimes present in the same histologic section. The latter phenomenon has implications for both the diagnosis of CIN and interpretation of “morphologic progression” from very low to high grade in the same case.     

内皮素—1与脑血管痉挛缺血性脑损害的关系及银杏叶制？…

目的：探讨内皮素－１（ｅｎｄｏｔｈｅｌｉｎ－１，ＥＴ－１）与蛛网膜下腔出血（ｓｕｂａｒａｃｈｎｏｉｄ ｈｅｍｏｒｒｈａｇｅ，ＳＡＨ）后缺血性脑损害的关系和银杏叶制剂（ｇｉｎｋｇｏ ｂｉｌｏｂａ ｅｘｔｒａｃｔ，ＧＢＥ）的防治作用。方法：应用非开颅大鼠模型，对ＳＡＨ组和ＧＢＥ组测量基底动脉（ｂａｓｉｌａｒ ａｒｔｅｒｙ，ＢＡ）管径并观察２４ｈ内微区脑血流量（ｒｅｇｉｏｎａｌ ｃｅｒｅｂｒａｌ ｂｌｏ     

针刺,中药对胆道“T”字管外引流胆汁量的影响

对９０例阻塞性黄疸患者作胆囊切除、胆总管切开取石及Ｔ字管外引流手术，术后随机分３组，分别予以耳针、中药及自然对照，结果针刺组和中药组在胆汁量、肝功能恢复及肛门排气恢复均优于对照组，说明针刺、中药结合手术治疗阻塞性黄疸效果优于单纯手术治疗。     

黄芪毛状根对造血系统功能的作用

本实验研究了黄芪毛状根对动物造血系统的作用,采用灌服给予黄芪毛状根5～20 g/kg,每日1次,连续5～12天,可提高失血性贫血小鼠的红细胞数、网织红细胞数,增加溶血性贫血小鼠的红细胞数、血红蛋白值、红细胞压积值,可对抗环磷酰胺、X射线所致小鼠的白细胞数和骨髓有核细胞数的下降。实验提示黄芪毛状根有促进造血系统功能的作用,其作用基本上与天然黄芪相似。     

Synovial sarcoma in childhood: Clinical and radiological findings

Objective: To study the clinical characteristics andradiological features of synovial sarcoma in childhood and its relation to the diagnosis and treatment. Methods: The clinical radiological features of 15 children with synovial sarcoma proved surgically and pathologically were analyzed. Results: In children, the tumor boundaries are poorly defined due to paucity of fat, and metastasis usually occurs early. Eight patients in this series had bone involvement, including: direct erosion by tumor causing cortical destruction, indirect pressure defect with sharp margin and reactive bone sclerosis and bone destruction of the primary intraosseous synovial sarcoma. Conclusion: The tumor is often misdiagnosed, the final confirmed diagnosis must be made by histological examination with imaging findings. It is emphasized that the patients should be treated with radiotherapy and chemotherapy preoperatively and postoperatively.     

P53 fusion protein expression in prokaryote and preparation of monoclonal antibody to P53

Objective: Conventional immunohistochemistry (IHC) is available to assess P⁵³ mutations, and expensive imported anti-P⁵³ monoclonal antibody has been used in China, it is necessary to study a new monoclonal antibody. Methods: The P⁵³ DNA fragment enconding N-terminal 180 amiao acide was obtained by PCR and was cloned into P^GEX-2T plasmid expressing glutathione S-transferase (GST). The P⁵³-GST fusion protein expressed by JM109 was used for immunizing BALB/C mice. We have raised one hybridoma strain secreting McAb to human P⁵³ (named M126). Results: The IHC analysis of 52 paraffin-embedded sections from human breast cancer with M126 and P_AB1801 (Zymed Co.) has showed that the positive immunoreactions were 25 cases (48%) and 22 cases (42.3%) respectively. The staining of M126 was stronger and preferable to P_AB1801. Conclusion: M126 can be instead of P_AB1801 for studying immunohistochemical analysis on P⁵³ protein.     

复发耐药性非霍奇金淋巴瘤小鼠肾包膜下移植肿瘤药敏测定的临床意义

目的:探讨小鼠肾包膜下移植肿瘤药敏测定(SRCA)在复发耐药NHL治疗中的作用.方法:对25例CHOP方案化疗后出现复发耐药的NHL病人进行了SRCA药敏测定,并依据SRCA结果进行化疗.结果:NHL肿瘤细胞在小鼠肾包膜下能够快速生长,移植成功率100%;复发耐药NHL对CTX、VCR、ADM表现为耐药,而对VP_(16)、MTX、DDP敏感性较高,其中对VP_(16)的敏感性显著高于CTX和VCR(P<0.05);依据药敏结果化疗,25例病人6例CR、13例PR,总有效率76%;其中21例接受由VP_(16)、MTX、DDP和强的松组成的方案治疗,6例CR,11例PR,有效率达81%.结论:NHL适宜用SRCA法进行药敏测定;复发耐药NHL的治疗可以VP_(16)、MTX和DDP为基础;化疗前进行SRCA药敏测定,能够减少用药盲目性,提高有效率.     

TCMP方案治疗对常规化疗耐药的复发转移性乳腺癌

目的:为评估由三苯氧胺、环磷酰胺、氨甲喋呤和顺铂组成的TCMP方案对常规化疗耐药后复发转移性乳腺癌的疗效.方法:25例对常规化疗耐药的复发转移性乳腺癌患者接受TCMP方案化疗.给药方法是三苯氧胺,10mg,口服,每天2次;环磷酸胺,0.8～1.0,静推,第1天;氨甲喋呤,40mg,肌注,第1、8天;顺铂40mm,静滴,第1～4天.23例患者有客观评价指标,均接受TCMP方案化疗最小2周期.结果:23例可评价患者中,总客观有效率为52.17%(95%可信区间31～73%),其中2例CR,10例PR.经Ridit检验治疗效果与雌激素受体状态无关.结论:以上提示TCMP方案对常规治疗耐药后复发转移性乳腺癌有较好疗效,三苯氧胺与以顺铂为主化疗方案在乳腺癌的治疗中有协同作用.     

腺病毒介导的肿瘤血管靶向的体外自杀基因治疗研究

本工作在血管内皮细胞中分别建立了HSV-tk/GCV和EC-cd/5-FC自杀基因系统,用于抑制实体肿瘤中血管生成的研究.构建含自杀基因的重组腺病毒AdCMVtk和AdCMVcd,滴度均1×10~(12)pfu/ml.病毒感染小鼠血管内皮细胞lGll(MOI=100),并鉴定自杀基因的表达,测定原药对它们的杀伤.生长抑制试验表明,GCV对lGll/Ad-tk细胞杀伤的IG_(50)为0.2μmol/ L,5-FC对lGll/Ad-cd细胞杀伤的IC_(50)为20μmol/L.电镜下显示被原药杀伤的血管内皮细胞有明显的细胞凋亡现象.同时混合细胞实验证明两系统均存在明显的旁杀伤效应.从而在体外水平证明了HSV-tk/GCV和EC-cd/5-FC自杀基因系统杀伤增生血管内皮细胞的有效性.     

人骨髓中自然抑制细胞对肿瘤细胞体外增殖的抑制作用

自然抑制(NS)活性是一类尚未完全定性的细胞所具有的对多种免疫反应的非特异抑制作用.NS细胞表面不存在成熟B细胞、T细胞及巨噬细胞的表面标记,为此,我们研究了NS细胞和造血干细胞(HSC)间的关系,结果证明,NS细胞是处于增殖周期中的HSC,并证明小鼠NS细胞具有抑制肿瘤细胞增殖的作用.本实验中,我们研究人骨髓中NS细胞的体外抗肿瘤活性,并确定其作用模式和生物学特性.