HPLC-ELSD及紫外分光光度法测定三七中皂苷的含量

采用反相高效液相色谱蒸发激光散射检测器测定三七中人参皂苷Rg1的含量。色谱柱为CLC-ODS(6．0mm×150mm),流动相为乙腈-水(3070),蒸发激光散射检测器。定量方法简单,准确。回收率为100．50％,RSD为1．82％。并用紫外分光光度法测定了三七中总皂苷的含量,结果和HPLC法基本一致,说明该方法定量准确,回收率为101．50％,RSD为1．44％。结论认为两方法均可以控制三七的质量。     

ClinicalStudy on Zhenjian Granule (针箭颗粒) in Improving Essential Hypertension and InsulinResistance

Objective: To observe the effect of Zhenjian Granule (ZJG) in improving essential hypertension and insulin resistance (IR).Methods: The 52 patients of hypertension were randomly divided into the ZJG group (n=32) and the control group (n=20), treated with ZJG and captopril respectively for 4 weeks. The changes of blood pressure, glucose, insulin, insulin sensitivity index (ISI) and blood lipid in them before and after treatment were determined and compared with those in normal group (17 healthy subjects).Results: Fasting insulin, blood glucose, and insulin 2 hrs after meal in hypertension patients before treatment were significantly higher but ISI was significantly lower than those in the normal control, indicating the existence of insulin resistance. After treatment, not only blood pressure in patients obviously lowered, but also levels of fasting insulin, blood glucose, and insulin 2 hrs after meal obviously lowered, while ISI markedly raised. IR was improved in both groups, and blood lipid were also improved in the ZJG group.Conclusion: ZJG was effective in reducing blood pressure, also it had significant effect in improving metabolic disorder, particularly on IR in patients of essential hypertension.     

国产替勃龙的激素活性及药效研究

为在国内开发治疗妇女更年期与手术后绝经症的新药 ,对国产替勃龙进行了多项生殖内分泌生物实验 ,结果表明该药兼具雌激素、孕激素及雄激素活性 ,并有延迟排卵作用 ,对大鼠雌激素缺乏性阴道炎也有治疗作用。其药理特性符合临床治疗女性更年期综合症要求。     

健胎液对人脐静脉内皮细胞分泌血管活性物质的影响

采用血清药理学方法观察健胎液对缺氧人脐静脉内皮细胞（HUVEC）分泌血管活性物质——内皮素（ET）、前列环素（PGI2)、一氧化氮（NO)的影响,以及对缺氧HUVEC形态结构的保护作用,旨在探讨健胎液防治胎儿宫内生长迟缓（IUGR）的机理。结果表明健胎液可显著提高缺氧HUVEC合成分泌PGI2、NO（P<0.05,P<0.01）,同时减少ET的释放(P<0.05);从超微结构上观察,健胎液能明显减轻缺氧对HUVEC细胞器和细胞核的损伤。结果提示健胎液通过增强HUVEC对缺氧的耐受性,调节血管活性物质的分泌是其防治胎儿宫内生长迟缓的机制之一。     

BCL-2,p53蛋白在乳腺原位癌中的表达

目的 :探讨BCL - 2 ,p53蛋白在乳腺原位癌及正常乳腺组织中的表达及相互关系。方法 :采用免疫组化检测 15例正常乳腺组织 ,10例小叶原位癌及 4 5例导管原位癌 (DuctalCarcinomainsitu ,DCIS)。结果 :BCL - 2蛋白在正常乳腺组织及小叶原位癌中的表达均阳性 ,p53蛋白均阴性 ,基因表型呈BCL - 2 /p53-。4 5例DCIS中 ,BCL - 2蛋白阳性率 77.8% (35/ 4 5) ,p53蛋白阳性率 2 4 .2 % (11/ 4 5)。BCL - 2蛋白表达与DCIS组织学分级密切相关 (P <0 .0 1)。DCIS中有 6 6 .7%呈BCL - 2 /p53-表型 ,多为高分化和中分化病例 ,还有 33.3% ,多为差分化病例 ,坏死出现率比呈BCL - 2 /p53-表型的病例高 (P <0 .0 1)。结论 :正常乳腺组织及小叶原位癌均呈BCL - 2 /p53-表型 ;6 6 .7%导管原位癌与正常乳腺组织具有相似的凋亡调控基因 (BCL - 2 /p53- ) ,非正常表型DCLS病例多代表分化差的病例 ,可能这些病例不受正常凋亡调控基因的控制 ,结果导致肿瘤细胞的存活和生长。     

胸腺因子D对实验性大鼠肝纤维化防治作用的研究

目的　研究胸腺因子D(TFD)的体内抗肝纤维化作用。方法　用四氯化碳 (CCL4 )复制大鼠肝纤维化模型 ;造模开始或造模 30d后予TFD ;实验结束后分别测定肝功能、纤维化指标 (Ⅲ型前胶原、透明质酸和层粘连素 )、肝组织中谷胱甘肽、超氧化物岐化酶及丙二醛含量 ,并作肝病理检查。结果　TFD可显著减轻CCl4 肝纤维化程度 ,能明显降低血清Ⅲ型前胶原、透明质酸、层粘连素和肝组织中丙二醛含量 ;显著提高谷胱甘肽和超氧化物岐化酶活性 ,改善肝功能 ,组织学检查亦显示具有抗肝纤维化作用。结论　TFD在体内具有抗肝纤维化的作用 ,可望用于肝纤维化的防治。     

Study on sensitivity of neuroglioma to chemotherapeutic drugs]

In this study, the chemosensitivity of 36 cases' fresh neuroglioma specimens was examined in vitro with MTT assay. The separation method for single tumor cell, the number of planting of cells, and the dosage and acting time of drugs, which have effects on the results of MTT assay, were studied systematically. On the basis of this experiment, we established a screening method for the chemosensitivity of neuroglioma; it is accurate, rapid, and simple. At the same time, the results showed that neuroglioma was more sensitive to the chemotherapeutic drugs Vm26, DDP and MMC, and the sensitivity varied with not only the drugs but also the individuals.     

CD3AK细胞冻融提取液的抗肿瘤活性研究

为研究ＣＤ３ＡＫ细胞冻融提取液的抗肿瘤作用,作者采集正常人外周血分离单个核细胞（ＰＢＭＣ）,先用抗ＣＤ３的单克隆抗体（ＣＤ３ＭｃＡｂ）的ｒＩＬ－２诱导和激活,使之成为ＣＤ３ＭｃＡｂ激活的杀伤细胞（ＣＤ３ＡＫ）,再制备ＣＤ３ＡＫ的冻融提取液,然后进行体内,外抗肿瘤实验。结果;低剂量的ＣＤ３ＭｃＡｂ和ｒＩＬ－２能明显诱导ＰＢＭＣ的活化和增殖;激活后的ＣＤ３ＡＫ细胞冻融提取液可抑制小鼠肝癌实体瘤细胞Ｈ２     

前方牵引器结全方丝弓矫治器矫治前牙反He

目的:进一步了解前方牵引矫治器对前牙反的矫治效果.方法:选择17例安氏Ⅲ类病人,男7例,女10例,最大12.8岁,最小8.5岁,均为替牙期及恒牙早期,X线头影测量显示主要为上颌后缩及下颌轻度前突,头颅侧位片上颈椎多数显示为生长发育高峰前期.结果:平均疗程9.5个月,X线头影测量结果骨骼改变主要为SNA角增大,平均增大2.33°,ANB角增大,平均增大4.17°,SNB角减小,平均减小1.97°.统计学处理有显著性差异.结论:前方牵引矫治器有促进上颌骨向前生长,抑制下颌骨向前生长的作用;而其口内装置选用方丝弓矫治器主弓丝上焊牵引钩配合矫形牵引,使之更为方便简捷.     

Pulmonary function changes following surgical correction for pectus excavatum

Ｐｅｃｔｕｓｅｘｃａｖａｔｕｍｉｓｔｈｅｍｏｓｔｆｒｅｑｕｅｎｔａｎｔｅｒｉｏｒｃｈｅｓｔｗａｌｌｄｅｆｏｒｍｉｔｙａｎｄｉｓｐｒｏｄｕｃｅｄｂｙｐｏｓｔｅｒｉｏｒｄｅｐｒｅｓｓｉｏｎｏｆｔｈｅｓｔｅｒｎｕｍａｎｄｌｏｗｅｒｃｏｓｔａｌｃａｒｔｉｌａｇｅ Ｉｔｏｃｃｕｒｓｍｏｒｅｃｏｍｍｏｎｌｙｉｎｂｏｙｓｂｙａｌｍｏｓｔａ 4∶ 1ｒａｔｉｏ Ｔｈｅｓｔｅｒｎｕｍａｎｄｃｏｓｔａｌｃａｒｔｉｌａｇｅｔｈａｔａｒｅａｎｇｌｅｄｐｏｓｔｅｒｉｏｒｌｙｍａｙｄｅｃｒｅａｓｅｔｈｅｃｈｅｓｔｖｏｌｕｍｅ,ｒ…