螺钉与经皮克氏针治疗Buechler2区Bennett骨折的2年随访

背景:治疗Bennett骨折方法颇多,对于较为多发的Buechler2区Bennett骨折目前尚无统一的治疗方案及评价标准。目的:对比切开复位螺钉内固定及经皮克氏针内固定对Buechler2区Bennett骨折固定后的功能恢复情况。方法:将Buechler2区Bennett骨折患者随机分为螺钉治疗组和克氏针治疗组,螺钉治疗组施行切开复位螺钉内固定,克氏针组施行闭合复位经皮交叉克氏针内固定。采用上肢功能评分评价其功能恢复情况。结果与结论:固定后、随访1年及随访2年,螺钉组上肢功能评分均低于克氏针组(P<0.05)。证实,短期内螺钉对于Buechler2区Bennett骨折的功能恢复优于克氏针。     

丹参多酚酸与三七总皂苷合用保护OGD/R损伤胶质细胞线粒体、促进神经因子表达作用研究*

目的 探讨丹参多酚酸（Salvianolate Acid，SAL）和三七总皂苷（Panax Notoginseng Saponins，PNS）合用保护氧糖剥夺/复氧复糖（Oxygen-Glucose Deprivation/Reoxygenation，OGD/R）损伤后星形胶质细胞线粒体、促进神经营养因子表达的作用及机制。方法 大鼠星形胶质细胞原代培养，建立OGD/R损伤模型。Cell counting kit-8（CCK-8）法检测星形胶质细胞活力，流式细胞仪检测活性氧（reactive oxygen species，ROS）释放量、胞内Ca2+ 浓度、线粒体膜电位（mitochondrial membrane potential，MMP）变化，实时定量PCR（real time polymerase chain reaction，RT-PCR）和蛋白质免疫印迹（western blot，WB）法检测HIF-1α、PI3K/Akt/mTOR信号通路蛋白及脑源性神经营养因子（brain-derived neurotrophic factor，BDNF）、神经生长因子（nerve growth factor，NGF）、胰岛素样生长因子（insulin-like growth factors，IGF-1α）的表达情况。结果 胶质细胞OGD/R条件确定为OGD4h/R24h，给药浓度确定为SAL 25 μg·mL-1、PNS 6.25 μg·mL-1。与OGD/R组相比，SAL 25 μg·mL-1、PNS 6.25 μg·mL-1、SAL 25 μg·mL-1与PNS 6.25 μg·mL-1合用均能增强损伤后星形胶质细胞活力（P＜0.05），升高MMP，降低ROS释放量（P＜0.05），降低损伤后星形胶质细胞HIF-1α蛋白和mRNA的表达，升高磷脂酰肌醇激酶（PI3K）磷酸化水平（P＜0.05）；SAL还可升高mTOR蛋白磷酸化水平（P＜0.05），PNS可提高Akt磷酸化水平（P＜0.05），增加损伤星形胶质细胞BDNF mRNA 表达（P＜0.05）；SAL、SAL与PNS合用能增加损伤后星形胶质细胞 BDNF、NGF、IGF-1α mRNA 表达（P＜0.05）。结论 丹参多酚酸和三七总皂苷组分合用可保护OGD/R损伤后星形胶质细胞线粒体、促进胶质细胞表达神经营养因子BDNF、NGF、IGF-1α。     

A Prenatal Ultrasound Scanning Approach: One-Touch Technique in Second and Third Trimesters

This study was aimed at evaluating the performance of the innovative technique Smart Fetus (SF) developed to recognize the planes and obtain the basic biometric measurements of fetuses automatically. This prospective study included 1005 uncomplicated singleton pregnancies undergoing routine examinations. For every pregnancy, planes, including the transverse section of the thalami, transverse section of the abdomen and longitudinal section of the femur, were acquired, and standard biometric measurements, including biparietal diameter, head circumference, abdominal circumference and femur length, were obtained using SF and traditional ultrasound technique (TUT). The accuracy, reproducibility and time required for the analysis of SF were compared with those of TUT. In 998 of 1005 cases (99.30%), SF successfully acquired the sections and made all measurements. The agreement between the techniques was high for all measurements. The time to obtain sections and measure biometric parameters or solely measure biometric parameters was significantly shorter with SF than with TUT. No significant differences were found in SF repeated measurements obtained by two independent observers. The SF technique helped in the acquisition of reliable standard sections and biometric measurements and saved time. It might serve as a novel ultrasound scanning approach and improve workflow efficiency.     

品管圈活动在提高“12345”工单办理作风满意度中的应用△

目的：探讨开展品管圈活动在提高“12345”工单办理的作风满意度中的效果。方法：依据品管圈活动的十大步骤，遵循PDCA原则，运用质量管理工具，对影响工单办理作风满意度的因素进行调查分析，拟定对策并实施，比较品管活动前后满意度情况。结果：工单办理的作风满意度由53.68%提升至82.35%，活动前后差异显著；同时，圈员们对品管手法的运用、自信心等均较活动前有所提高。结论：通过品管活动的实施，提高了工单办理的作风满意度，对工单办理工作有了标准化的作业要求，提高了工作的效果与效率，也增加了圈员们对品管手法知识的掌握，增强品管工具运用的能力。     

CBX7mRNA在卵巢癌组织中的表达及意义

目的研究CBX7mRNA在正常卵巢组织和卵巢肿瘤组织中的表达及意义。方法利用Real-TimePCR方法检测CBX7mRNA在70例卵巢正常组织和肿瘤组织中的表达并分析其与分期、分化、组织类型的相关性。结果CBX7mRNA在卵巢肿瘤组织中的表达明显低于卵巢正常组织。根据FIGO分期，CBX7mRNA在I、Ⅱ期的表达高于Ⅲ、Ⅳ期，表达差异具有统计学意义（P〈0．05）。在卵巢恶性肿瘤组织中，高、中分化组的表达高于低分化组，表达差异具有统计学意义（P〈0．05）。结论CBX7mRNA的低表达可能与卵巢癌的发生发展有关。     

Relation Between Cellular Senescence and Liver Diseases

Cellular senescence refers to a process that cellular proliferation and differentiation modulated by the multiple stimulating factors gradually decline.Aging cells present the irreversible stop of proliferation and differentiation and change in secretory function because the cell cycle of aging cells is steadily blocked at some point. It has have been shown that cellular senescence plays an important role in the occurrence and development of liver diseases. In this paper, we review the advances in relations between cellular senescence and liver diseases.     

PDCA循环在医院后勤管理中实例分析

应用PDCA循环法对微波炉集中统一管理进行效益分析。通过PDCA在实际工作中的实例分析,探讨PDCA循环管理模式在医院后勤管理实践中的运用。运用PDCA循环对微波炉实行集中管理进行实例分析,是一种行之有效的管理方法,杜绝了安全隐患,降低了维修维护费用,在短期内取得显著管理效果。     

宫颈腺癌的研究进展

宫颈腺癌是宫颈癌的一种特殊病理类型,较鳞癌少见.近年来,宫颈腺癌的发病率及其在宫颈癌中所占比例不断上升,且发病趋于年轻化,预后比同期鳞癌差.此外,宫颈腺癌卵巢转移率高于鳞癌,对放、化疗的敏感性显著低于鳞癌,这些均对宫颈腺癌的诊治有重要影响.从宫颈腺癌致病因素(雌激素、孕激素与人乳头瘤病毒)、卵巢转移高危因素及卵巢保留以及放化疗进展3个热点问题着手,简述近年来宫颈腺癌的研究进展.