60例老年乳腺癌的临床病理特点及分子分型

目的 探讨老年女性乳腺癌临床病理特点及分子分型.方法 回顾性分析郑州人民医院2011年1月至2016年8月收治的60例60岁以上(包括60岁)老年女性乳腺癌及同期随机选取的60例青年乳腺癌(年龄≤35岁)的临床及病理资料.结果 60例老年女性乳腺癌中临床分期Ⅰ期12例,Ⅱ期44例,Ⅲ期4例;浸润性导管癌49例,有腋窝淋巴结转移32例;雌激素受体阳性48例,孕激素受体阳性43例,CerbB-2受体阳性表达18例;与青年组比较,在临床分期、肿块大小及ER、PR受体阳性表达差异均有统计学意义(P值分别为0.028、0.040、0.044、0.038);而在淋巴结有无转移、病理类型及CerbB-2受体阳性表达上差异均无统计学意义(P值分别为0.199、0.178、0.404);老年组LuminalA 29例、Luminal B 19例、TNBC(三阴性乳腺癌)5例、CerbB-2过表达7例,与青年组乳腺癌分子分型比较,P值为0.035.结论 老年乳腺癌具有独特的生物学特点,临床分期以Ⅰ、Ⅱ期为主,ER、PR受体阳性较多,病理类型以浸润性导管癌为主,分子分型以Luminal A、Luminal B较多.     

A natural lipopeptide of surfactin for oral delivery of insulin

Abstract

Surfactin, a natural lipopeptide produced by Bacillus, is gaining attention for potentially biomedical and pharmaceutical applications. Here, surfactin was assayed for oral delivery of insulin (INS) by its ability to bind to and promote protein to penetrate through the cell membrane. Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, surfactin was found to form co-precipitates with INS to protect it from acidic and enzymatic attack in the gastrointestinal tract. Further analysis by non-reductive electrophoresis showed surfactin could bind to INS forming heteropolymers. Analysis with circular dichroism, we found this binding significantly influenced the INS structure with decreased rigid α-helix and β-turn, but with increased flexible β-sheet and random coil. The change with more flexible structure was favorable for INS to penetrate through the cell membrane. Fluorescence spectra analysis also showed surfactin could lead Phe and Tyr in the inner of INS exposed outside, further promoting INS permeabilization by improving the hydrophobic-lipophilic interactions between INS and cell membrane. As a result, the effective permeability (Peff) of INS plus surfactin was 4.3 times of that of INS alone. In vivo assay showed oral INS with surfactin displayed excellent hypoglycemic effects with a relative bioavailability of 12.48% and 5.97% in diabetic mice and non-diabetic dogs, respectively. Summary, surfactin is potential for oral delivery of INS by its role as an effective protease inhibitor and permeability enhancer.     

获取高质量生物样品图象的电镜技术浅析

获取高分辨率和高反差的生物样品电镜图象受许多因素的影响,它包括切片厚度的选择,电镜的校直合轴、加速电压的选择、以及聚焦方法和曝光时间等。除生物样品制备技术这一重要因素外,必须考虑到上述各因素间的相互关系及其综合选择、然后才有可能获得高质量的生物样品的超微结构图象。     

张琪以加味甘露饮治疗慢性肾功能衰竭的经验分析

张琪教授是全国著名中医临床学家,精于仲景学说,对金元四大家、明清各家学派及温病学术理论有高深造诣。临证善治多种疑难病症,如肾病、心病、神志病、肝病、风湿病、糖尿病等,但尤对慢性肾脏病有较深造诣,治疗方法独具特色。     

活血化瘀强度与气血双亏型胃癌转移率相关性机理探讨

本文回顾研究了益气生血方剂中活血化瘀强度与气血双亏型胃癌转移率之间的相关性,显示两者有较强的正相关,提示气血双亏型胃癌转移率随活血化瘀作用加强而增高。推测可能与本型胃癌血小板数量及聚集性下降、血浆纤维蛋白含量低及凝血抑制、纤溶增强易于肿瘤转移有关,且大多数活血化瘀方药具有抑制血小板聚集,抗血凝和增加纤溶的现代药理机制。进而建议气血双亏型胃癌,在血小板数量及聚集率明显下降、凝血时间明显延长及FDP高时,尽量降低方剂中活血化瘀强度,以避免促进肿瘤转移。     

不同产地金银花挥发油GC-MS的比较分析

金银花为忍冬科植物忍冬 Lonicera japonicaThunb.的干燥花蕾 ,具有清热解毒、凉散风热的功效。金银花分布广泛 ,山东和河南是我国传统的道地产区 ,而产地不同 ,其有效成分含量存在着较大差异 [1,2 ] 。有关挥发油的成分研究曾有报道[3～ 5] ,为全面了解其挥发油成分的差异及变化 ,提高金银花的质量 ,我们采用 GC- MS首次对山东、河南及江苏等地金银花的挥发油成分做了比较分析。1　实验部分1 .1　样品及挥发油的提取 :样品分别采自山东平邑、日照 ,河南封丘、新密 ,江苏南京等地 ,经鉴定为L.japonica Thunb.。称取新鲜的花蕾 1 0 0 g,…     

大蒜素对T细胞激活的影响

大蒜素在高浓度(50μg／ml)时对T细胞激活有抑制作用。但在适当浓度(3．125～12．5μg／ml)时则对T细胞激活有促进作用，这种促进作用与大蒜素抑制巨噬细胞产生NO的能力有关。大蒜素能够对抗S180细胞和艾氏暖水癌细胞产生的肿瘤免疫抑制因子对T细胞激活的抑制作用。提示大蒜素在肿瘤治疗中可能有较大的应用价值。     

老年慢性阻塞性肺病治验一得

本文秉承姚培发老中医"肾精虚衰是人体衰老和老年病的主要因素"之学术观点.认为老年慢性阻塞性肺病中多见脾肾两虚,痰瘀内阻,尤其阳气不足,三焦气化不利.更易痰饮停聚,为咳为喘.总结剖析老年慢性阻塞性肺病医案二则.提出三条主张:1.补肾健睥为治本之重点;2.注重扶阳.阴阳平衡;3.久病宜益气活血祛瘀.     

Study on modified shengmai yin injection for prevention and treatment of brain impairment in endotoxin shock rats

Objective:To examine the effects of modified Shenmai Yin on invigorating vital energy, promoting blood flow, and protection against neural impairment in an endotoxin-induced shock rat model. Methods: Ninety-six SD rats were randomly divided into four groups: sham operation (saline 20 ml/kg), shock model (lipopolysaccharide, LPS, 8 mg/kg), Reformed Shengmai Yin (加味生脉饮 Pulse-activating Decoction) (LPS 8 mg/kg + reformed Shengmai Yin Injection 10 ml/kg), and dexamethasone (LPS 8 mg/kg + dexamethasone 5 mg/kg) groups. Each group was subdivided into 1 h, 2 h, 3 h, and 6 h time points for observation. The carotid artery was separated and connected with a biological functional system to monitor mean arterial pressure (MAP). Brain water levels, malonaldehyde (MDA) content, and superoxide dismutase (SOD) activity were also determined. Results: In the shock model group, MAP was progressively decreased after injection of LPS, brain water and MDA contents were increased, brain SOD activity was decreased, and capillary vessel edema in brain tissue was also observed. All these parameters were improved significantly in both treatment groups, although the effects were more marked with Shengmai Yin than with dexamethasone. Conclusion: Modified Shengmai Yin exhibits strong anti-shock and neuroprotective effects against Endotoxininduced shock.