全文获取类型
收费全文 | 2311篇 |
免费 | 180篇 |
国内免费 | 9篇 |
专业分类
耳鼻咽喉 | 13篇 |
儿科学 | 62篇 |
妇产科学 | 33篇 |
基础医学 | 352篇 |
口腔科学 | 68篇 |
临床医学 | 213篇 |
内科学 | 504篇 |
皮肤病学 | 17篇 |
神经病学 | 233篇 |
特种医学 | 88篇 |
外国民族医学 | 5篇 |
外科学 | 306篇 |
综合类 | 37篇 |
一般理论 | 6篇 |
预防医学 | 264篇 |
眼科学 | 26篇 |
药学 | 159篇 |
中国医学 | 3篇 |
肿瘤学 | 111篇 |
出版年
2023年 | 18篇 |
2022年 | 19篇 |
2021年 | 42篇 |
2020年 | 25篇 |
2019年 | 29篇 |
2018年 | 46篇 |
2017年 | 35篇 |
2016年 | 37篇 |
2015年 | 31篇 |
2014年 | 62篇 |
2013年 | 102篇 |
2012年 | 139篇 |
2011年 | 142篇 |
2010年 | 75篇 |
2009年 | 55篇 |
2008年 | 129篇 |
2007年 | 129篇 |
2006年 | 127篇 |
2005年 | 98篇 |
2004年 | 100篇 |
2003年 | 91篇 |
2002年 | 108篇 |
2001年 | 55篇 |
2000年 | 58篇 |
1999年 | 52篇 |
1998年 | 37篇 |
1997年 | 21篇 |
1996年 | 19篇 |
1995年 | 30篇 |
1994年 | 19篇 |
1993年 | 22篇 |
1992年 | 50篇 |
1991年 | 50篇 |
1990年 | 57篇 |
1989年 | 39篇 |
1988年 | 39篇 |
1987年 | 31篇 |
1986年 | 19篇 |
1985年 | 21篇 |
1984年 | 16篇 |
1983年 | 19篇 |
1982年 | 15篇 |
1981年 | 14篇 |
1979年 | 20篇 |
1978年 | 14篇 |
1976年 | 11篇 |
1974年 | 14篇 |
1973年 | 13篇 |
1971年 | 10篇 |
1970年 | 12篇 |
排序方式: 共有2500条查询结果,搜索用时 15 毫秒
91.
Michael Theisen Will Roeffen Susheel K. Singh Gorm Andersen Linda Amoah Marga van de Vegte-Bolmer Theo Arens Régis Wendpayangde Tiendrebeogo Sophie Jones Teun Bousema Bright Adu Morten H. Dziegiel Michael Christiansen Robert Sauerwein 《Vaccine》2014
Effective control and eventual eradication of malaria drives the imperative need for clinical development of a malaria vaccine. Asexual parasite forms are responsible for clinical disease and death while apathogenic gametocytes are responsible for transmission from man to mosquito. Vaccines that combine antigens from both stages may provide direct protection and indirect benefit by reducing the force of infection. We constructed a chimeric antigen composed of a fragment of the Plasmodium falciparum (Pf) glutamate-rich protein fused in frame to a correctly folded fragment of Pfs48/45. The chimera was produced in Lactococcus lactis and induced robust antibody responses in rodents to the individual components. Specific antibodies showed strong transmission blocking activity against multiple Pf-strains in the standard membrane feeding assay and functional activity against asexual stages in the antibody dependent cellular inhibition assay. The combined data provide a strong rationale for entering the next phase of clinical grade production and testing. 相似文献
92.
Craig Barnes Binam Bajracharya Matthew Cannalte Zakir Gowani Will Haley Taha Kass-Hout Kyle Hernandez Michael Ingram Hara Prasad Juvvala Gina Kuffel Plamen Martinov J Montgomery Maxwell John McCann Ankit Malhotra Noah Metoki-Shlubsky Chris Meyer Andre Paredes Jawad Qureshi Xenia Ritter Philip Schumm Mingfei Shao Urvi Sheth Trevar Simmons Alexander VanTol Zhenyu Zhang Robert L Grossman 《J Am Med Inform Assoc》2022,29(4):619
ObjectiveThe objective was to develop and operate a cloud-based federated system for managing, analyzing, and sharing patient data for research purposes, while allowing each resource sharing patient data to operate their component based upon their own governance rules. The federated system is called the Biomedical Research Hub (BRH).Materials and MethodsThe BRH is a cloud-based federated system built over a core set of software services called framework services. BRH framework services include authentication and authorization, services for generating and assessing findable, accessible, interoperable, and reusable (FAIR) data, and services for importing and exporting bulk clinical data. The BRH includes data resources providing data operated by different entities and workspaces that can access and analyze data from one or more of the data resources in the BRH.ResultsThe BRH contains multiple data commons that in aggregate provide access to over 6 PB of research data from over 400 000 research participants.Discussion and conclusionWith the growing acceptance of using public cloud computing platforms for biomedical research, and the growing use of opaque persistent digital identifiers for datasets, data objects, and other entities, there is now a foundation for systems that federate data from multiple independently operated data resources that expose FAIR application programming interfaces, each using a separate data model. Applications can be built that access data from one or more of the data resources. 相似文献
93.
94.
95.
96.
97.
98.
Hans Jürgen Netter Hans Herbert Guldner Carin Szostecki Heinz-Jürgen Lakomek Hans Will 《Arthritis \u0026amp; Rheumatology》1988,31(5):616-622
A human liver complementary DNA expression library was screened using sera from patients with high titers of autoantibodies, to search for clones expressing major autoantigens that are relevant in connective tissue diseases. One of the clones isolated expressed a major epitope(s) that was immunoreactive with anti-U1 RNP sera, as shown by several techniques. Affinity-purified autoantibodies from the cloned RNP protein specifically recognized the 68-kd U1 RNP protein of HeLa cell nuclear extracts. All sera containing anti-U1 RNP antibodies detected by immunodiffusion, counterimmuno-electrophoresis, or immunoblotting also recognized the cloned RNP protein. The RNP antigen-expressing bacterial colonies and the partially purified cloned RNP fusion protein have been applied to fast and sensitive immunologic assays for the detection and quantification of anti-U1 RNP antibodies. 相似文献
99.
Jon B. Toledo Matthias Arnold Gabi Kastenmüller Rui Chang Rebecca A. Baillie Xianlin Han Madhav Thambisetty Jessica D. Tenenbaum Karsten Suhre J. Will Thompson Lisa St. John-Williams Siamak MahmoudianDehkordi Daniel M. Rotroff John R. Jack Alison Motsinger-Reif Shannon L. Risacher Colette Blach Joseph E. Lucas Rima Kaddurah-Daouk 《Alzheimer's & dementia》2017,13(9):965-984
Introduction
The Alzheimer's Disease Research Summits of 2012 and 2015 incorporated experts from academia, industry, and nonprofit organizations to develop new research directions to transform our understanding of Alzheimer's disease (AD) and propel the development of critically needed therapies. In response to their recommendations, big data at multiple levels are being generated and integrated to study network failures in disease. We used metabolomics as a global biochemical approach to identify peripheral metabolic changes in AD patients and correlate them to cerebrospinal fluid pathology markers, imaging features, and cognitive performance.Methods
Fasting serum samples from the Alzheimer's Disease Neuroimaging Initiative (199 control, 356 mild cognitive impairment, and 175 AD participants) were analyzed using the AbsoluteIDQ-p180 kit. Performance was validated in blinded replicates, and values were medication adjusted.Results
Multivariable-adjusted analyses showed that sphingomyelins and ether-containing phosphatidylcholines were altered in preclinical biomarker-defined AD stages, whereas acylcarnitines and several amines, including the branched-chain amino acid valine and α-aminoadipic acid, changed in symptomatic stages. Several of the analytes showed consistent associations in the Rotterdam, Erasmus Rucphen Family, and Indiana Memory and Aging Studies. Partial correlation networks constructed for Aβ1–42, tau, imaging, and cognitive changes provided initial biochemical insights for disease-related processes. Coexpression networks interconnected key metabolic effectors of disease.Discussion
Metabolomics identified key disease-related metabolic changes and disease-progression-related changes. Defining metabolic changes during AD disease trajectory and its relationship to clinical phenotypes provides a powerful roadmap for drug and biomarker discovery. 相似文献100.