全文获取类型
收费全文 | 5905篇 |
免费 | 307篇 |
国内免费 | 60篇 |
专业分类
耳鼻咽喉 | 109篇 |
儿科学 | 270篇 |
妇产科学 | 130篇 |
基础医学 | 490篇 |
口腔科学 | 236篇 |
临床医学 | 538篇 |
内科学 | 1352篇 |
皮肤病学 | 106篇 |
神经病学 | 280篇 |
特种医学 | 123篇 |
外科学 | 934篇 |
综合类 | 400篇 |
一般理论 | 4篇 |
预防医学 | 403篇 |
眼科学 | 188篇 |
药学 | 415篇 |
1篇 | |
中国医学 | 75篇 |
肿瘤学 | 218篇 |
出版年
2023年 | 86篇 |
2022年 | 273篇 |
2021年 | 397篇 |
2020年 | 189篇 |
2019年 | 205篇 |
2018年 | 229篇 |
2017年 | 151篇 |
2016年 | 186篇 |
2015年 | 255篇 |
2014年 | 298篇 |
2013年 | 307篇 |
2012年 | 445篇 |
2011年 | 415篇 |
2010年 | 253篇 |
2009年 | 220篇 |
2008年 | 325篇 |
2007年 | 301篇 |
2006年 | 312篇 |
2005年 | 304篇 |
2004年 | 237篇 |
2003年 | 244篇 |
2002年 | 178篇 |
2001年 | 52篇 |
2000年 | 55篇 |
1999年 | 36篇 |
1998年 | 33篇 |
1997年 | 25篇 |
1996年 | 27篇 |
1995年 | 13篇 |
1994年 | 15篇 |
1993年 | 8篇 |
1992年 | 23篇 |
1991年 | 18篇 |
1990年 | 19篇 |
1989年 | 13篇 |
1988年 | 17篇 |
1987年 | 14篇 |
1986年 | 5篇 |
1985年 | 11篇 |
1984年 | 6篇 |
1983年 | 6篇 |
1981年 | 6篇 |
1980年 | 6篇 |
1979年 | 5篇 |
1977年 | 6篇 |
1974年 | 6篇 |
1973年 | 4篇 |
1972年 | 4篇 |
1970年 | 5篇 |
1969年 | 4篇 |
排序方式: 共有6272条查询结果,搜索用时 15 毫秒
31.
Abdullah Al-Mitwalli Grigorios Kyriazis Omar El-Taji Elizabeth Chandra Wearmouth Deborah Phillipa Burns Youssef Fady Matthew Simms Smith Nicholas 《Current Urology》2021,15(2):115
Background:Urosepsis is a recognized complication of transrectal ultrasound-guided prostate biopsy (TRUS-Bx). Pre-biopsy rectal swabs have been used to identify patients with microorganisms in the rectal flora resistant to the conventionally used empirical prophylaxis. The transperineal route of biopsy (TP-Bx) has a lower complication risk but comes at an increased cost.Materials and methods:Retrospective cohort study including patients undergoing prostate biopsies between October/2015 and April/2018. The intervention cohort, a rectal swab was performed, the result of which dictated the biopsy route; TRUS-Bx against TP-Bx. TP-Bx for patients with fluoroquinolone resistance or extended-spectrum β-lactamase. The control cohort underwent TRUS without a rectal swab receiving empirical antibiotics—oral ciprofloxacin and intravenous gentamicin.Results:Total 1000 patients were included in which 500 underwent a swab, 14 (2.8%) developed post-TRUS biopsy infective complications with 3 having positive bacteremia (0.6%); 500 had no swab, 47 (9.4%) developed post-TRUS biopsy infective complications with 22 (4.4%, p < 0.05) having positive bacteremia. Three patients (0.6%) of patients who underwent swab developed urinary tract infection symptoms whilst 12 (2.4%) had urinary tract infection in the control group. In those patients that underwent a swab, 14 required hospitalization with mean length of stay of 2.5 days versus 43 patients of the control with 3.6 days. Cost analysis concluded savings of this strategy was £18,711.Conclusions:We have demonstrated a protocol that reserves template biopsies for higher risk patients and can significantly reduce sepsis and other infectious complication rates whilst also proving to be a cost-efficient strategy. We recommend that units not utilizing rectal swabs to uncover the fluoroquinolone resistance rate by introducing them. We advocate units that already utilize rectal swabs, to introduce transperineal biopsy for their higher risk patients. 相似文献
32.
1. We have investigated the differences between the nitric oxide synthase inhibitor L-NMMA, the guanylate cyclase inhibitor methylene blue and the potassium channel blockers apamin and charybdotoxin or apamin and iberiotoxin, in their abilities to increase vasoconstrictor responses in rat small mesenteric arterial rings. 2. When administered during the maintained contraction to PGF2alpha (10 microM), L-NMMA (100 microM) or the combination of apamin (0.7 microM) and charybdotoxin (0.1 microM) significantly increased the contractile response. Methylene blue (10 microM) increased the contraction, but this did not reach significance. However, apamin (0.7 microM) and iberiotoxin (0.1 microM) also significantly increased the contractile response. 3. The combination of L-NMMA or methylene blue with apamin/charybdotoxin produced significantly greater increases in the contractile response to PGF2alpha than achieved individually. 4. Relaxations to acetylcholine (10 microM) were significantly reduced by L-NMMA or methylene blue but not by apamin in combination with charybdotoxin or iberiotoxin. 5. Since apamin/iberiotoxin had similar effects to apamin/charybdotoxin, it is likely that the actions of these agents involve direct actions on smooth muscle potassium channels rather than inhibition of endothelium-derived hyperpolarising factor (EDHF). These results suggest that endothelium-derived nitric oxide but not EDHF has a major role in modulating vascular tone under these conditions. 相似文献
33.
参麦注射液对内皮细胞增殖和迁移的影响 总被引:7,自引:0,他引:7
目的:探讨参麦注射液对血管生成的影响。方法:采用MTT法检测参麦注射液对牛血清和肿瘤细胞条件培养液促进的牛主动脉内皮细胞增殖的影响,采用琼脂糖刮除法检测参麦对牛血清和肿瘤条件培养液促进的牛内皮细胞迁移的影响。结果:在含10%新生小牛血清培养液中和在肿瘤细胞条件培养液中,参麦都能明显抑制牛主动脉内皮细胞增殖,且呈量效关系,对肿瘤细胞条件培养液诱导的内皮细胞迁移抑制作用显著。方法:参麦能抑制牛内细胞增殖和迁移,具有抑制血管生成的作用。 相似文献
34.
目的 对恶性腹水患者血清白细胞介素2(IL-2)水平进行测定,了解血清IL-2水平与腹腔灌注IL-2治疗疗效之间的关系,以期获得判定恶性腹水IL-2治疗疗效的指标。方法 54例恶性非肝癌性腹水患者均接受IL-2腹腔灌注治疗,治疗前应用ELISA法检测血清IL-2水平,将IL-2测定值较高的27例患者分为A组,IL-2测定值较低的27例患者分为B组。比较两组患者的治疗效果。结果 血清IL-2测定值较低组IL-2腹腔灌注治疗的有效率为77.8%(21/27),明显高于IL-2测定值较高组的51.9%(14/27,P<0.05)。结论 恶性腹水患者IL-2腹腔灌注治疗前血清IL-2水平高低与IL-2治疗的疗效明显相关,原有IL-2水平较低的患者IL-2腹腔灌注治疗的疗效较好。血清IL-2水平可作为判定IL-2腹腔灌注治疗疗效的指标。 相似文献
35.
目的探讨膀胱癌细胞nm23蛋白表达量与丝裂霉素药物敏感性之间的相关性.为临床膀胱移行细胞癌治疗方案选择提供理论依据.方法对32例临床病理诊断为膀胱移行细胞癌标本采用S-P免疫组化和图像分析技术检测细胞的nm23蛋白表达量;同时采用MTT法测定膀胱癌细胞对丝裂霉素的药物敏感性.结果膀胱癌nm23表达量与MMC抗药指数相关,相关指数为0.890(P<0.001).结论膀胱癌nm23蛋白表达量与MMC抗药指数呈正相关. 相似文献
36.
37.
Objective To analyse the genome of influenza A (H1N1) vires so as to elucidate its molecular characteristics and evolution status. Methods DNA sequences of the influenza viruses were collected from NCBI, and compared with the genomes of referenced intluenza viruses. The phylogenetic trees were constructed by the neighbor-joining method, and the pathogenicity, drug susceptibility and vaccine protection were analyzed. Results Phyiogenetic analysis showed that the genes encoding HA, PB2, PBI, PA, NP, and NS protein were most closely related to those influenza A viruses circulating in swine populations in North America. NA and M gene belonged to Eurasia lineages swine influenza vires. The amino acid sequence of the cleavage site between HA1 and HA2 was PARSSR ↓ GLFGAI with the typical characteristics of the low pathogenic influenza virus. Influenza A(H1N1) virus can spread from person-to-person. It is sensitive to oseltamivir and zanamivir but resistant to amantadine and remantadine. The current human seasonal influenzavaccines confered little protection against influenza A/H1N1 because of the great diversity on antigenic domains between A/H1N1 virus and vaccine virus. Conclusions Influenza A(H1N1) virus is a reassortant virus of North America and Eurasia hneages swine influenza virus. It is important to develop a vaccine against the currently circulating virus strain to control the disease spread. 相似文献
38.
目的:研制胸腺肽α1(Tα1)单克隆抗体,为Tα1作用机制的阐明奠定基础。方法:用戊二醛将Tα1和牛血清白蛋白进行交联,以其交联物为抗原,按常规淋巴细胞杂交瘤技术制备单克隆抗体,并采用间接酶联免疫吸附(ELISA)法和Western免疫印迹来鉴定单克隆抗体的特异性。结果:建立了分泌抗Tα1抗体的杂交瘤细胞株,能特异性地与Tα1结合,但与单纯牛血清白蛋白、酵母抽提物和转染Tα1酵母未诱导表达产物无反应。其抗体效价为1:10240,Ig亚类为IgG2b。结论:本研究建立了针对Tα1高效价、高特异性单克隆抗体细胞株。 相似文献
39.
Abdullah MH Van Arsdell GS Hornberger LK Adatia I 《The Annals of thoracic surgery》2000,70(6):94-2149
We report a patient with stenosis of the native ascending aorta after palliation of hypoplastic left heart syndrome and aortic atresia. We describe the approach to diagnosis, temporary support with extracorporeal membrane oxygenation, and successful surgical reintervention. Stenosis of the native ascending aorta is an important, potentially reversible cause of acute, early postoperative ventricular dysfunction. 相似文献
40.
目的:比较胃粘膜活检组织快速尿素酶试验和改良的Giemsa染色诊断幽门螺杆菌(Hp)感染的异同。方法:对413例接受胃镜检查的患者,同时利用快速尿素酶试验和改良的Giemsa染色诊断胃粘膜幽门螺杆菌感染,规定两者均阳性为真阳性,两者均阴性为真阴性,快速尿素酶试验阳性而Giemsa染色阴性为假阳性,快速尿素酶试验阴性而Giemsa染色阳性为假阴性。结果:两者诊断结果一致者有311例(75.3% ) ,其中真阳性225例,真阴性86例,两项检查结果符合率为75.3% ;假阴性54例(13.07% ) ,假阳性48例(11.63% )。结论:快速尿素酶试验检测Hp感染需结合组织学诊断结果综合分析。 相似文献