HPLC法测定复方板蓝根颗粒中靛玉红的含量

目的:建立复方板蓝根颗粒中靛玉红的含量测定方法.方法:C18柱, 以甲醇-0.1%磷酸溶液(65∶35)为流动相,检测波长为289 nm.结果:供试品中靛玉红得到很好的分离与测定,其线性范围为0.02224～0.2224 μg(r＝0.9998),平均回收率为99.31%(n=5),RSD为2.54%.结论:该方法简便准确,重现性好,可用于复方板蓝根颗粒的质量控制.     

莪术不同炮制品镇痛抗炎作用研究

摘要:目的:通过不同的致痛、致炎模型观察莪术不同炮制品的镇痛抗炎作用.方法:采用小鼠热板法和扭体法,观察其镇痛作用;采用耳肿法及毛细血管通透性法观察药物的抗炎作用.结果:莪术不同饮片对二甲苯所致的耳廓肿胀及醋酸所致的毛细血管通透性增加都有明显的抑制作用.其中以醋煮莪术作用较强.莪术不同炮制品对醋酸所致的扭体也有明显的抑制作用,热板法显示各样品均能明显提高小鼠的痛阈值,其中以醋煮莪术作用较为明显.结论:莪术的不同炮制品均有显著的镇痛抗炎作用,其中以醋煮莪术作用较强.     

Clinicopathologic and genetic profile of intracranial marginal zone lymphoma: a primary low-grade CNS lymphoma that mimics meningioma.

PURPOSE: Although rare overall, marginal zone B-cell lymphoma (MZBCL) is the most common primary low-grade CNS lymphoma reported in the literature. The aim of this study is to elucidate the biology and genetic features of this unusual tumor. PATIENTS AND METHODS: Fifteen CNS MZBCLs were studied clinically, pathologically, and genetically, including fluorescent in situ hybridization analyses with commercially available MALT1 and IgH break-apart and centromere 3, 7, 12, and 18 probes. RESULTS: CNS MZBCLs preferentially affect middle-aged women (female-to-male ratio, 4:1), with 93% presenting as dural-based masses mimicking meningioma. Ten patients with 1 to 7.6 years of follow-up after diagnosis showed no evidence of disease after radiation and/or chemotherapy. Like MZBCLs outside of the CNS, they consisted of CD20+, CD3- small B lymphocytes with varying degrees of plasmacytic differentiation and predominantly kappa light-chain restriction (78%). Lymphoid follicles with follicular colonization were seen in three patients and deposition of amyloid was noted in samples from two patients, one of which was tumefactive. Neither Bcl-6 protein nor Epstein-Barr virus-encoded RNA was expressed. Trisomy 3 was detected in six of 12 patients, with no rearrangements of MALT1 or IgH and no trisomies of 7, 12, or 18 detected. CONCLUSION: Our data suggest that intracranial MZBCL is an indolent primary CNS lymphoma that typically presents as a meningioma-like dural-based mass. Trisomy 3, but not MALT1 or IgH translocation, is a common genetic abnormality that may contribute to the pathogenesis of this CNS lymphoma.     

老年原发性高血压患者血压昼夜节律与靶器官损害临床观察

目的探讨老年原发性高血压患者血压昼夜节律与靶器官损害的关系.方法入选年龄≥60岁老年原发性高血压病患者150例,通过24小时动态血压监测了解其血压昼夜节律变化,根据血压昼夜节律监测结果为非杓型组(夜间平均血压较白昼平均血压下降值＜10%)78例,另一对照组为杓型72例.所有入选患者检查:超声心动图、颈动脉超声、肌酐清除率、尿微量白蛋白.结果非杓型组的尿微量白蛋白定量和左心室质量指数均高于杓型组(p＜0.01);非杓型组的颈动脉内膜厚度大于杓型组(P＜0.05);左室射血分数和肌酐清除率小于杓型组(P＜0.05).结论血压昼夜节律消失是老年原发性高血压患者靶器官损害的重要危险因素.     

龙岩市中老年人群骨质疏松的流行病学调查

目的:分析龙岩市中老年人群骨密度(BMD)及骨质疏松(OP)患病率变化规律,为骨质疏松预防提供科学依据.方法:随机对龙岩市40岁以上的人群进行BMD测定,受试者共176人,准确记录受试者性别、年龄,使用美国Lunar公司的DEXA测定L2～4及髋部的BMD,然后进行比较和统计分析.结果:男性的BMD以50岁组或60岁组最低;女性的BMD随年龄的增加而明显下降,但70岁组下降平缓;除了40岁组外,同龄组男性BMD大于女性BMD.男性各年龄组腰椎(L2～4)的骨质疏松症患病率均低于女性各组,且较女性迟10年.结论:女性在40岁以后应监测BMD的变化,男性在50岁以后应监测BMD的变化,60岁组应为男女性BMD监测的重点.龙岩市中老年人群应普及预防知识,加强锻炼,增加户外活动,以减少OP的发生.     

白花前胡提取物对大脑中动脉梗塞大鼠IL-6及IL-8的影响

目的:研究白花前胡对大脑中动脉梗塞后相关病理生理改变及对脑组织及血清IL-6和IL-8水平的影响.方法:线栓法制备大脑中动脉梗塞大鼠模型,研究白花前胡对大脑中动脉梗塞性大鼠脑缺血后行为障碍及脑梗死范围、缺血组织病理形态的影响及脑组织及血清IL-6和IL-8水平的影响.结果:阻断大脑中动脉(MCA)后,所有动物都出现程度不同的运动障碍,大鼠脑组织和血清IL-6及IL-8水平均显著升高,白花前胡水醇提取液可明显抑制大脑中动脉梗塞大鼠血清中IL-6及IL-8水平;4 mg*ml-1和2 mg*ml-1白花前胡水醇提取液可明显改善大脑中动脉梗塞模型大鼠神经症状分值、降低脑梗死范围.结论:白花前胡水醇提取物能有效抑制级联反应中炎性细胞因子IL-6 及IL-8的产生,减轻或消除脑缺血级联反应中的炎症反应,发挥保护神经元,减少梗死面积的作用.     

热量限制对成年弱视小鼠初级视皮质突触可塑性的调控作用

目的：探讨热量限制（CR）对成年单眼形觉剥夺（MD）弱视小鼠视皮质突触可塑性的调控作用及可能的分子机制。方法：实验研究。将54只新生健康昆明小鼠按随机数字表法分为正常组、MD+自由进食（AL）组、MD+CR组,每组18 只。小鼠21 日龄时构建MD模型,35 日龄时去除剥夺因素,63 日龄时通过行为学检测视敏度及电生理检测视功能;免疫组织化学及Western Blot法检测视皮质组织中可塑性相关蛋白突触后致密蛋白95（PSD95）、突触素（SYP）、生长相关蛋白43（GAP-43）等的表达;RT-PCR检测胰岛素样生长因子1（IGF-1）的表达。采用重复测量双因素方差分析比较各组数据差异。结果：最终正常组、MD+AL组、MD+CR组分别有14、18、18 只小鼠完成研究。从第1 周开始,MD+CR组小鼠体质量百分比的增加明显低于MD+AL组（P<0.05）。MD+AL组小鼠视敏度明显低于正常组和MD+CR组小鼠的视敏度（P<0.05）。MD+AL组小鼠PSD95、GAP-43、SYP的表达均明显低于正常组和MD+CR组小鼠（P<0.05）。MD+AL组小鼠视皮质中IGF-1 mRNA水平较正常组和MD+CR组显著降低（P<0.05）。结论：CR能改善成年MD弱视小鼠视觉功能,增加视皮质中可塑性相关蛋白的表达,重新激活视皮质可塑性,其机制可能与其调节IGF-1的表达有关。     

Incremental value of live/real time three‐dimensional transesophageal echocardiography over the two‐dimensional technique in the assessment of a tuberculoma involving the left atrium and appendage

Intracardiac tuberculomas are extremely rare, and cardiac involvement in tuberculosis accounts for only 0.5% of extrapulmonary tuberculosis. We report for the first time incremental value of live/real time three‐dimensional transesophageal echocardiography over two‐dimensional transesophageal echocardiography in the assessment of a tuberculoma involving the left atrium and left atrial appendage.