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91.
Objective To construct a eukaryotic expression system with pcDNA3-PfCSP/Hela for the Circ umsporozoite protein (CSP) gene of Plasmodium falciparum (P.falciparum), t o observe the immune responses in BALB/c mice induced by the expressed proteins .Methods The recombinant plasmid pcDNA3-PfCSP was transformed into the Hela cell line. The expressed protein was isolated and analyzed by using SDS-PAGE and used for immunization of BALB/c mice by subcutaneous, intravenous, and intraperitone al adminstration.Enzyme-linked immunosorbent assay(ELISA), Dot-ELISA, Wester n blot, T lymphocyte proliferation test, natural killer cell(NKC) activity assay , and CD4(+) and CD8(+) T cell detection were used for observation of humoral an d cellular immune responses.Results Immune sera strongly reacted with the expressed protein, antibody titer was up to 1∶6400 as detected by ELISA.Western blot analysis revealed a specific b and at 38.3 Kda.When the spleen cells of normal and immunized BALB/c mice we re specifically stimulated with expressed protein, the optical densities were 0 .12±0.03 and 0.34±0.04, respectively.The latter were significantly highe r than the former (P<0.01).We used the MTT colorimetric assay to measure NKC activity of mice spleen.The results showed that the NKC activity of immuni zed BALB/c mice was remarkably higher than that of the controls (P<0.05). CD4(+) and CD8(+) T cells were detected by using monoclonal antibody immunofluor escence methods.The results showed that the percentage of CD4(+) and CD8(+) T cells of immunized group were significantly higher than that of control group ( P<0.05).Conclusions The humoral and cell-mediated immune responses and elevated NKC activity to pr oducts made with a eukaryotic expression system could be specifically detected i n BALB/c mice.These findings indicate that the expressed protein could enhance the immune function in mice. 相似文献
92.
0 引言 我科 1996 / 1998分别应用消痔灵与强的松龙混合液、消痔灵液、强的松龙液行鼻息肉内 ,鼻息肉蒂部注射治疗鼻息肉各 5 0例 ,并设对照组为鼻腔滴入及口服类固醇激素 5 0例 ,合计 2 0 0例 ,观察并对比其疗效 .1 对象和方法1.1 对象 男 12 8例 ,女 72例 ,年龄 8~ 78(平均 38)岁 ,病程 32 a~ 45 (平均 4.5 ) a.其中在本次治疗前做过一次鼻息肉摘除术后复发的 2 7例 ,做过 2次或 2次以上手术的 12例 .主要症状为鼻塞、流脓涕、头痛及嗅觉减退 .全部病例治疗前均行鼻窦 X线拍片 ,其中上颌窦炎 12 5例、筛窦炎 5 8例、蝶窦炎 2例、… 相似文献
93.
冷冻同种异体骨段移植修复股骨近段大块骨缺损 总被引:1,自引:0,他引:1
为观察大块冷冻同种异体骨移植后免疫功能的改变及预后,对13例恶性骨肿瘤瘤段切除后的骨缺损10.8~19.4cm(平均为14.6cm),分别采用冻冷异体松质骨段移植的髋关节加压融合术(6例)和异体股骨近段半关节移植成形术(7例)修复.10例分别于术前4天及术后14和28天检测其空腹外周血各项免疫学指标,以及术后3、6、9、12、24、48个月进行99mSPECT骨扫描(5例)和X-线片检查(12例),以观察宿主的免疫反应和异体骨愈合情况.结果:①外周血T淋巴细胞亚群(OKT3+,OKT4+,OKT8+)及血清补体(C3,C4)和循环免疫复合物(CiC)手术前后比较无显著性差异(P>0.05).②99mTe SPECT骨扫描显示:术后3~6个月移植的异体骨两端及髓腔内同位素浓集明显低于正常,而异体骨两端所对应的自体骨端同位素浓集明显高于正常,此现象于植入后9个月开始减弱,可达数年.X线片显示:异体骨愈合率为92.3%(12/13),2例移植的异体松质骨段于术后4~6年完全成活替代.以上可见:①冻冷异体骨段移植后宿主的全身免疫机能改变不明显,其反应是以局部炎症为主的细胞免疫过程;②异体骨可做为修复大块骨缺损的良好材料. 相似文献
94.
喉功能保留的梨状窝癌外科治疗 总被引:25,自引:0,他引:25
目的 评价喉功能保留梨状窝癌的手术治疗效果。方法 回顾性分析了1974~1994年6月我院初治的44例经保留喉功能手术治疗的梨状窝癌病例,其中T110例T218例,T42例。2例术后放射治疗,42例术前放射治疗,原发灶术式包括:36例梨状窝切除术;8例喉及下咽部分切除术。结果 44例5年生存率为50%,I期为80%,Ⅱ期为71.43%,Ⅲ期为52.9%,Ⅳ期为26.7%,总的局部控制率为81.8% 相似文献
95.
Huimin Cheng Liangbing Fu Xia Yang Yujian Yang Zhening Zhang Yuan Tao Junting Wan Zhengchao Tu Jianxin Chen Yingjun Li 《Journal of medical virology》2023,95(1):e28327
Quinolin-2-one represents an important and valuable chemical motif that possesses a wide variety of biological activities; however, the anti-influenza activities of quinolin-2-one-containing compounds were rarely reported. Herein, we describe the screening and identification of 3-aryl-quinolin-2-one derivatives as a novel class of antiviral agents. The 3-aryl-quinolinone derivatives were synthesized via an efficient copper-catalyzed reaction cascade that we previously developed. Using this synthetic method, preliminary structure–activity relationships of this scaffold against the influenza A virus infection were systematically explored. The most potent compound 34 displayed IC50 values of 2.14 and 4.88 μM against the replication of H3N2 (A/HK/8/68) and H1N1 (A/WSN/33) strains, respectively, without apparent cytotoxicity on MDCK cells. We further demonstrated that 27 and 34 potently inhibited the plaque formation of the IAV, rendering this scaffold attractive for pursuing novel anti-influenza agents. 相似文献
96.
DeLima M Amato RJ Jackson A Tu SM Banks M Finn L Ellerhorst J 《Cancer biotherapy & radiopharmaceuticals》1997,12(6):365-370
We have completed a phase I/II trial to evaluate the toxicity and efficacy of the combination of gamma interferon and 5-fluorouracil in metastatic renal cell carcinoma. Gamma interferon was administered at a weekly dose of 100 micrograms. 5-fluorouracil was given as a 5 day continuous infusion days 1-5 of each 21 day cycle. In the phase I portion of the trial, the gamma interferon dose was held constant, while the 5-fluorouracil was escalated from 500 to 800 mg/m2/day. Serum neopterin and beta 2 microglobulin were measured prior to and 48 hours after each dose of gamma interferon for the first six weeks of treatment. Dose limiting toxicity was not encountered in the phase I part of the trial; therefore the phase II study was initiated at the 800 mg/m2/day dose of 5-fluorouracil. No responses were seen among 34 patients treated on the phase II trial. Forty-six percent of patients experienced disease stabilization and the remainder progressed through treatment. In the phase I trial, increments in neopterin and beta 2 microglobulin levels differed significantly between patients treated with lower and higher doses of 5-fluorouracil. We conclude that the addition of 5-fluorouracil to gamma interferon does not appear to enhance the cytokines clinical activity. Incremental increases in macrophage activation markers with escalating 5-fluorouracil doses suggests a role for 5-fluorouracil beyond its usual proposed cytotoxic activity and warrants further investigation into potential immunologic effects of this drug. 相似文献
97.
About 30% of patients who have a Roux-en-Y gastrojejunostomy after gastrectomy suffer from abdominal pain, nausea, vomiting
of food and bloating made worse by eating. This syndrome, called the Roux stasis syndrome, is caused, in part, by a motility
disorder of the Roux limb. Transection of the jejunum during the construction of the limb separates the limb from the natural
small intestinal pacemaker located in the duodenum. Ectopic pacemakers then appear in the limb and trigger retrograde contractions
in its proximal portion. These contractions slow transit through the limb and result in Roux stasis. Current nonsurgical treatment
of the syndrome includes the use of prokinetic agents and intestinal pacing, neither of which has demonstrated long-term benefits.
A near-total gastrectomy may speed upper gastrointestinal transit somewhat, but stasis in the Roux limb often persists. Our
current approach aims at preventing the syndrome by the use of an ‘uncut’ Roux limb, an operation which preserves myoneural
continuity between the duodenal pacemaker and the Roux limb and so prevents the appearance of ectopic pacemakers and stasis
in the limb. 相似文献
98.
《中国海洋药物》杂志刊载论文的引文分析 总被引:1,自引:0,他引:1
采用文献计量学方法,对1989年-1993年《中国海洋药物》杂志刊载论文的引文进行了分析。结果表明,此间共刊载论文181篇,共有引文1301条,每篇论文平均引文量为7.98条。自引率为12.76%,在全部引文中,期刊占65.10%,图书占27.60%,其中占7.30%;语种分布中文占65.72%,英文占29.13%,日文占5.07%,俄文占0.08%。10年文献累计利用率,中文为83.27%,外文 相似文献
99.
<正> 随着创建卫生城市的开展,垃圾处理日益受到重视,无锡市建立的垃圾处理厂,对垃圾处理采用二次发酵工艺,即“好氧堆肥发酵”、“自然堆肥贮存发酵”。现将发酵结果报道如下。1 材料与方法1.1 实验室条件下(温度26±1℃,相对湿度60%±5%)饲养家蝇1龄蛆500条,家蝇蛹500只。1.2 透气塑料筒20只(自制),编号1~20号1.3 垃圾处理厂的垃圾处理采用2次发酵,第1次机械通风好氧堆肥发酵10d,取出后入库,再第2次自然堆肥贮存发酵10d。 相似文献
100.