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991.
992.
Tseng-tong Kuo Tse-Ching Chen Li-Yu Lee Pei-Hsuan Lu 《Journal of cutaneous pathology》2009,36(10):1069-1073
Background: Cutaneous Rosai-Dorfman disease (CRDD) shares the histopathological features of abundant plasma cells and stromal fibrosis with IgG4-related sclerosing disease. The possible role of IgG4+ plasma cells in CRDD was investigated.
Methods: Twelve cases of CRDD were reviewed, and their lesions were immunostained with anti-IgG4 and anti-IgG antibodies. The number of IgG4+ and IgG+ plasma cells and their ratios were estimated. Serum IgG4 and IgG concentrations were measured in two recent cases.
Results: Many IgG4+ and IgG+ plasma cells were found in all 12 cases. IgG4+ plasma cells ranged from 21 to 204 per high-power field (HPF) (mean 117/HPF), and IgG+ plasma cells ranged from 114 to 759/HPF (mean 349/HPF). All cases had more than 30 IgG4+ cells/HPF, except one case. The IgG4/IgG ratio ranged from 16% to 51% (mean 34%). Serum IgG4 concentration and serum IgG4/IgG ratio were increased in one recent case. Various degrees of stromal fibrosis were present in all cases.
Conclusions: The presence of many IgG4+ plasma cells and stromal fibrosis suggests that CRDD may be related to IgG4-related sclerosing disease. Many IgG4+ plasma cells is another feature of CRDD, and serum IgG4 may be elevated. 相似文献
Methods: Twelve cases of CRDD were reviewed, and their lesions were immunostained with anti-IgG4 and anti-IgG antibodies. The number of IgG4+ and IgG+ plasma cells and their ratios were estimated. Serum IgG4 and IgG concentrations were measured in two recent cases.
Results: Many IgG4+ and IgG+ plasma cells were found in all 12 cases. IgG4+ plasma cells ranged from 21 to 204 per high-power field (HPF) (mean 117/HPF), and IgG+ plasma cells ranged from 114 to 759/HPF (mean 349/HPF). All cases had more than 30 IgG4+ cells/HPF, except one case. The IgG4/IgG ratio ranged from 16% to 51% (mean 34%). Serum IgG4 concentration and serum IgG4/IgG ratio were increased in one recent case. Various degrees of stromal fibrosis were present in all cases.
Conclusions: The presence of many IgG4+ plasma cells and stromal fibrosis suggests that CRDD may be related to IgG4-related sclerosing disease. Many IgG4+ plasma cells is another feature of CRDD, and serum IgG4 may be elevated. 相似文献
993.
Objectives
Intracranial arterial stenosis (IAS) is a severe disease with a high recurrent stroke rate even under the best medical treatment.
Statins have been demonstrated to prevent stroke and to slow or halt atherosclerosis progression. This study was performed
to observe the effect of atorvastatin on the progression of IAS, explore the factors associated with atherosclerosis regression
and the recurrent rate of stroke.
Methods
A hospital-base observation study enrolled 40 stroke patients with middle cerebral artery (MCA) or/and basilar artery (BA)
stenosis. All participants had hyperlipidemia and were given atorvastatin 40 mg per day for at least six months. IAS was assessed
by magnetic resonance angiogram (MRA) at the time of enrollment and then at least six months later. The primary outcome was
the progression of IAS. All patients were also given antiplatelet agents for stroke prevention.
Results
At the end of the study, 23 (58 %), 15 (38 %) and 2 (4 %) patients had regressed, stationary and progressed IAS, respectively.
Females were likely to have regressed IAS. The recurrent stroke rate was 18 %. Among the 54 stenotic vessels, 29 (54 %) vessels
were assessed as improvement in stenosis.
Conclusion
Compared with other studies, more regressed, stationary IAS and less progressed IAS were found in our study. Female gender
was likely to have regressed IAS after statin treatment. Further clinical outcome trials are required to assess the effects
of such therapy on morbidity and mortality in this particular group of patients. 相似文献
994.
Porcine neonatal pancreatic cell clusters (NPCCs) isolated from 1- to 3-day-old pigs cured diabetic nude mice more than 14 weeks after transplantation. To shorten the latent period between transplantation and reversal of hyperglycemia, we investigated the effects of insulin-like growth factor-1 (IGF-1) and NPCCs isolated from 1-month-old pigs after transplantation. Pig pancreata were cut into fragments, collagenase digested, and then cultured. Three hundred and 2000 NPCCs were transplanted under the kidney capsule of nondiabetic and diabetic nude mice, respectively. After transplantation, the graft-bearing kidneys were removed to measure insulin content. NPCCs isolated from 1- to 3-day-old pigs were cultured with or without IGF-1 for 6 days. The stimulation index was not significantly different between the 2 groups at 1, 2, or 4 weeks. Moreover, at 4 weeks after transplantation of 300 NPCCs to nondiabetic nude mice yielded comparable graft insulin content as the recipients of NPCCs precultured with or without IGF-1. Two thousand cultured NPCCs isolated from 1-to 3-day-old pigs or 1-month-old pigs were transplanted into diabetic nude mice. The blood glucose levels of diabetic recipients in both groups decreased at the same rate after transplantation, achieving normoglycemia at 8 weeks. The graft insulin content at 12 weeks was not different between the 2 groups. Our data indicated that isolated NPCCs cultured with IGF-1 showed no beneficial effects on insulin secretion and transplantation; NPCCs isolated from 1-to 3-day-old and 1-month-old pigs displayed similar effects on transplantation. 相似文献
995.
Differential roles of hypogastric and pelvic nerves in the analgesic and motoric effects of vaginocervical stimulation in rats 总被引:1,自引:0,他引:1
S. Tiffany Cunningham Judith L. Steinman Beverly Whipple Anne D. Mayer Barry R. Komisaruk 《Brain research》1991,559(2):337-343
Bilateral transection of the pelvic and/or hypogastric nerves, which convey afferent activity from the reproductive tract, was performed to ascertain the role of these nerves in the analgesic and motoric effects of vaginocervical mechanostimulation (VS) in rats. Two indices of analgesia were used: tail flick latency to radiant heat (TFL) and vocalization threshold to electrical shock of the tail (Voc-T). Nerve cuts were performed at least one week prior to behavioral testing. Bilateral transection of both the pelvic and hypogastric nerves eliminated the analgesic effects of VS on the TFL and Voc-T tests. Bilateral transection of only the pelvic nerves reduced the number of rats showing maximal VS-induced elevation in TFL, without altering the effect of VS on Voc-T. By contrast, bilateral transection of only the hypogastric nerves attenuated the Voc-T-elevating effect of VS, without reducing the effect of VS on elevating TFL. The effects of VS on producing immobility, hindlimb extension and blockage of hindlimb withdrawal to foot pinch were eliminated by combined bilateral pelvic and hypogastric neurectomy. However, bilateral transection of either nerve alone did not significantly alter the efficacy of VS in producing these effects. These findings indicate that the pelvic and hypogastric nerves contribute to the immobility- and extensor-inducing, and flexor-inhibiting effects of VS, and differentially mediate the analgesia-producing effects of VS. 相似文献
996.
The safety and efficacy of recombinant DNA produced human growth hormone in the treatment of growth failure in prepubertal children with idiopathic or organic deficiency of pituitary GH has been assessed. Five patients entered this clinical trial. They had never been treated with hGH, anabolic steroids or any medicine that affected GH and all of them were healthy without any chronic disease; except patient 1, who took a surgical operation for cerebellar astrocytoma at the age of 3. Each patient was treated with subcutaneous injection of recombinant somatropin (SAIZEN) at a dosage of 0.2 IU/Kg +/- 10% three times per week in the evening for 1 year. Typical catch up growth was observed. The height increased by between 6.3 and 15.1 cm and their mean growth velocity of 3.7 cm/yr prior to therapy increased to 11.1 cm/yr during one year of treatment. The annual change in bone age during the treatment with SAIZEN was 2.0 +/- 0.6 years in four patients, except patient 2 who showed different bone age (right hand 3.5 years, left hand 6.0 years). Anti-hGH antibodies were observed in patient 1, but the binding capacity was low (1:10), and no clinical symptoms or growth attenuation occurred. No side effects or laboratory abnormalities were noted throughout the clinical trial. In conclusion, recombinant somatropin has a growth promoting effect and low immunogenicity, and it is shown to be safe and effective during the first year of therapy in children with GH deficiency. 相似文献
997.
Hsin‐Tzu Liu An‐Bang Liu Michael B. Chancellor Hann‐Chorng Kuo 《BJU international》2009,104(8):1158-1162
OBJECTIVE
To measure urinary nerve growth factor (uNGF, essential in nerve growth and regeneration) levels in patients with a cerebrovascular accident (CVA), to determine whether uNGF could be a biomarker for predicting the neurological deficits in CVA, as the level of uNGF increases in patients with idiopathic detrusor overactivity (DO) and incontinence.PATIENTS, SUBJECTS AND METHODS
uNGF levels were measured using an enzyme‐linked immunosorbent assay in normal subjects and patients with CVA and different severities of neurological impairment. Total uNGF levels were normalized to the concentration of urinary creatinine (uNGF/Cr).RESULTS
The median (interquartile range) uNGF/Cr levels were significantly higher in patients, at 0.13 (0–1.04), than in normal subjects (undetectable). The uNGF/Cr levels correlated well with the severity of neurological impairment. Patients with none/minimal neurological impairment had no detectable uNGF/Cr level, like the controls. Patients with mild/moderate impairment had levels of 0.27 (0.09–0.8) and with severe impairment level of 1.53 (0.5–3.0) (both P < 0.001), significantly greater than that of none/minimal impairment or controls. However, uNGF/Cr levels were not correlated with age, location of CVA, multiplicity of CVA, duration of CVA, urodynamic findings or the presence of urge urinary incontinence.CONCLUSIONS
The uNGF level is correlated with the severity of neurological impairment in patients with CVA but not with urge symptoms or urodynamic findings, suggesting elevated uNGF might be a result of the neurological lesion rather than lower urinary tract dysfunction in CVA. 相似文献998.
999.
Chiung-Nien Chen MD PhD Cheng-Chi Chang PhD Ting-En Su Wen-Ming Hsu MD PhD Yung-Ming Jeng MD Ming-Chih Ho MD PhD Fon-Jou Hsieh MD Po-Huang Lee MD PhD Min-Liang Kuo PhD Hsinyu Lee PhD King-Jen Chang MD PhD 《Annals of surgical oncology》2009,16(2):524-533
The purpose of this study was to identify genes of interest for a subsequent functional and clinical cohort study using complementary
(c)DNA microarrays. cDNA microarray hybridization was performed to identify differentially expressed genes between tumor and
nontumor specimens in 30 gastric cancer patients. Subsequent functional studies of the selected gene were carried out, including
cell cycle analysis, cell migration analysis, analyses of vascular endothelial growth factor (VEGF) and placenta growth factor
(PlGF), and oligo-microarray studies using two pairs of stable cell lines of the selected gene. Another independent cohort
study of 79 gastric cancer patients was conducted to evaluate the clinical significance of the selected gene in human gastric
cancer. Calreticulin (CRT) was selected for further investigation. Two pairs of stable cell lines of CRT overexpression and
CRT knockdown were constructed to perform functional studies. CRT enhanced gastric cancer cell proliferation and migration.
Overexpressed CRT upregulated the expression and secretion of PlGF and VEGF. CRT had a reciprocal effect on connective tissue
growth factor (CTGF) expression. Positive immunohistochemical staining of calreticulin was significantly correlated with high
microvessel density (MVD)
(p = 0.014), positive serosal invasion (p = 0.013), lymph node metastasis (p = 0.002), perineural invasion (p = 0.008), and poor patient survival (p = 0.0014). Multivariate survival analysis showed that CRT, MVD, and serosal invasion were independent prognosticators. We
conclude that CRT overexpression enhances angiogenesis, and facilitates proliferation and migration of gastric cancer cells,
which is in line with the association of CRT with MVD, tumor invasion, lymph node metastasis, and survival in gastric cancer
patients.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
1000.
A simple approach to 1,1′,2,2′,3,3′,4,4′-octahydro-1,1′-biisoquinolines is described. Reaction of phenethylamines with oxalyl chloride led to N,N′-bis(phenethyl) oxamides (1). Cyclization of oxamides by using Bischler – Napieralski conditions gave 3,3′,4,4′-tetrahydro-1,1′-biisoquinoline(3) and unusual products 2, 4, 5. Reduction of 3,3′,4,4′-tetrahydro-1,1′-biisoquinolines with sodium boron hydride gave both rac-1,1′,2,2′,3,3′,4,4′-octahydro-1,1′-biisoquinolines(6) and meso-1,1′,2,2′,3,3′,4,4′ -octahydro-1,1′-biisoquinolines(7). Compound 6 was resolved to (1S, 1S′) (8) and (1R, 1R′) (9) furtherly. By treating all the biisoquinolines with K2PtCl4 afforded their cis-dichloridoplatinum (II) complexes (12–18). The antitumor activity of these complexes was evaluated. 相似文献