全文获取类型
收费全文 | 14896篇 |
免费 | 1025篇 |
国内免费 | 21篇 |
专业分类
耳鼻咽喉 | 90篇 |
儿科学 | 499篇 |
妇产科学 | 385篇 |
基础医学 | 1900篇 |
口腔科学 | 94篇 |
临床医学 | 2161篇 |
内科学 | 2742篇 |
皮肤病学 | 188篇 |
神经病学 | 1359篇 |
特种医学 | 317篇 |
外科学 | 1403篇 |
综合类 | 182篇 |
一般理论 | 17篇 |
预防医学 | 2086篇 |
眼科学 | 187篇 |
药学 | 1008篇 |
中国医学 | 15篇 |
肿瘤学 | 1309篇 |
出版年
2023年 | 99篇 |
2022年 | 155篇 |
2021年 | 336篇 |
2020年 | 225篇 |
2019年 | 380篇 |
2018年 | 413篇 |
2017年 | 316篇 |
2016年 | 335篇 |
2015年 | 393篇 |
2014年 | 516篇 |
2013年 | 786篇 |
2012年 | 1205篇 |
2011年 | 1186篇 |
2010年 | 621篇 |
2009年 | 588篇 |
2008年 | 949篇 |
2007年 | 1105篇 |
2006年 | 1039篇 |
2005年 | 1047篇 |
2004年 | 930篇 |
2003年 | 889篇 |
2002年 | 764篇 |
2001年 | 113篇 |
2000年 | 80篇 |
1999年 | 138篇 |
1998年 | 163篇 |
1997年 | 120篇 |
1996年 | 96篇 |
1995年 | 112篇 |
1994年 | 90篇 |
1993年 | 74篇 |
1992年 | 50篇 |
1991年 | 41篇 |
1990年 | 46篇 |
1989年 | 32篇 |
1988年 | 43篇 |
1987年 | 40篇 |
1986年 | 33篇 |
1985年 | 25篇 |
1984年 | 44篇 |
1983年 | 29篇 |
1982年 | 44篇 |
1981年 | 43篇 |
1980年 | 29篇 |
1979年 | 19篇 |
1978年 | 20篇 |
1977年 | 16篇 |
1976年 | 24篇 |
1975年 | 15篇 |
1974年 | 14篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
71.
Ansar M Din MA Arshad M Sohail M Faiyaz-Ul-Haque M Haque S Ahmad W Leal SM 《European journal of human genetics : EJHG》2003,11(1):77-80
Autosomal recessive nonsyndromic deafness is one of the most frequent forms of inherited hearing impairment. Over 30 autosomal recessive nonsyndromic hearing loss loci have been mapped, and 15 genes have been isolated. Of the over 30 reported autosomal recessive nonsyndromic hearing loss (NSHL) loci, the typical phenotype is prelingual non-progressive severe to profound hearing loss with the exception of DFNB8, which displays postlingual onset and DFNB13, which is progressive. In this report we describe a large inbred kindred from a remote area of Pakistan, comprising six generations and segregating autosomal recessive nonsyndromic prelingual deafness. DNA samples from 24 individuals were used for genome wide screen and fine mapping. Linkage analysis indicates that in this family the NSHL locus, (DFNB35) maps to a 17.54 cM region on chromosome 14 flanked by markers D14S57 and D14S59. Examination of haplotypes reveals a region that is homozygous for 11.75 cM spanning between markers D14S588 and D14S59. A maximum two-point LOD score of 5.3 and multipoint LOD score of 7.6 was obtained at marker D14S53. The interval for DFNB35 does not overlap with the regions for DFNA9, DFNA23 or DFNB5. 相似文献
72.
Marcie R Finney Nicholas J Greco Stephen E Haynesworth Joseph M Martin David P Hedrick Jimmy Z Swan Daniel G Winter Suzanne Kadereit Matthew E Joseph Pingfu Fu Vincent J Pompili Mary J Laughlin 《Biology of blood and marrow transplantation》2006,12(5):585-593
Endothelial precursor cells (EPCs) cultured from adult bone marrow (BM) have been shown to mediate neovasculogenesis in murine models of vascular injury. We sought to directly compare umbilical cord blood (UCB)- and BM-derived EPC surface phenotypes and in vivo functional capacity. UCB and BM EPCs derived from mononuclear cells (MNC) were phenotyped by surface staining for expression of stromal (Stro-1, CXCR4, CD105, and CD73), endothelial (CD31, CD146, and vascular endothelial [VE]-cadherin), stem cell (CD34 and CD133), and monocyte (CD14) surface markers and analyzed by flow cytometry. The nonobese diabetic/severe combined immunodeficiency murine model of hind-limb ischemia was used to analyze the potential of MNCs and culture-derived EPCs from UCB and BM to mediate neovasculogenesis. Histologic evaluation of the in vivo studies included capillary density as a measure of neovascularization. Surface CXCR4 expression was notably higher on UCB-derived EPCs (64.29%+/-7.41%) compared with BM (19.69%+/-5.49%; P=.021). Although the 2 sources of EPCs were comparable in expression of endothelial and monocyte markers, BM-derived EPCs contained higher proportions of cells expressing stromal cell markers (CD105 and CD73). Injection of UCB- or BM-derived EPCs resulted in significantly improved perfusion as measured by laser Doppler imaging at days 7 and 14 after femoral artery ligation in nonobese diabetic/severe combined immunodeficiency mice compared with controls (P<.05). Injection of uncultured MNCs from BM or UCB showed no significant difference from control mice (P=.119; P=.177). Tissue samples harvested from the lower calf muscle at day 28 demonstrated increased capillary densities in mice receiving BM- or UCB-derived EPCs. In conclusion, we found that UCB and BM-derived EPCs differ in CXCR4 expression and stromal surface markers but mediate equivalent neovasculogenesis in vivo as measured by Doppler flow and histologic analyses. 相似文献
73.
Constance Schrander-Stumpel Christine de Die-Smulders Marc de Krom Suzanne Schyns-Fleuren Ben Hamel Deni Jaeken Jean-Pierre Fryns 《Clinical genetics》1993,43(6):303-308
Schrander-Stumpel C, de Die-Smulders C, de Krom M, Schyns-Fleuren S, Hamel B, Jaeken D, Fryns J-P. Marden-Walker syndrome: case report, literature review and nosologic discussion.
Clin Genet 1993: 43: 303–308. © Munksgaard, 1993
The Marden-Walker syndrome is characterized by psychomotor retardation, a mask-like face with blepharophimosis, micrognathia and a high-arched or cleft palate, low-set ears, kyphoscoliosis and joint contractures. We report on a male patient with the clinical features of the syndrome. In addition, he had a Dandy-Walker malformation with hydrocephalus and vertebral abnormalities. During pregnancy, there were feeble fetal movements and polyhydramnios. We propose that Marden-Walker syndrome is one of the etiologic possibilities in children with the heterogeneous fetal a(hypo)kinesia deformation sequence (FADS). Differential diagnosis is discussed. The etiology is probably heterogeneous. 相似文献
Clin Genet 1993: 43: 303–308. © Munksgaard, 1993
The Marden-Walker syndrome is characterized by psychomotor retardation, a mask-like face with blepharophimosis, micrognathia and a high-arched or cleft palate, low-set ears, kyphoscoliosis and joint contractures. We report on a male patient with the clinical features of the syndrome. In addition, he had a Dandy-Walker malformation with hydrocephalus and vertebral abnormalities. During pregnancy, there were feeble fetal movements and polyhydramnios. We propose that Marden-Walker syndrome is one of the etiologic possibilities in children with the heterogeneous fetal a(hypo)kinesia deformation sequence (FADS). Differential diagnosis is discussed. The etiology is probably heterogeneous. 相似文献
74.
Gilissen LJ Bolhaar ST Matos CI Rouwendal GJ Boone MJ Krens FA Zuidmeer L Van Leeuwen A Akkerdaas J Hoffmann-Sommergruber K Knulst AC Bosch D Van de Weg WE Van Ree R 《The Journal of allergy and clinical immunology》2005,115(2):364-369
BACKGROUND: Apple allergy is dominated by IgE antibodies against Mal d 1 in areas where birch pollen is endemic. Apples with significantly decreased levels of Mal d 1 would allow most patients in these areas to eat apples without allergic reactions. OBJECTIVE: The aim of this study was to inhibit the expression of Mal d 1 in apple plants by RNA interference. METHODS: In vitro -grown apple plantlets were transformed with a construct coding for an intron-spliced hairpin RNA containing a Mal d 1-specific inverted repeat sequence separated by a Mal d 1-specific intron sequence. The presence of the construct in transformants was checked by PCR. Expression of Mal d 1 in leaves was monitored by prick-to-prick skin testing in 3 patients allergic to apples and by immunoblotting with a Mal d 1-reactive mAb and with IgE antibodies against Mal d 1. RESULTS: After transformation, plantlets were selected on the basis of having a normal phenotype and growth rate. With PCR, in 6 of 9 selected plantlets, the presence of the gene-silencing construct was demonstrated. By skin prick test it was shown that a wild-type plantlet had significantly ( P < .05) higher allergenicity than 5 of the transformants. Reduction of expression of Mal d 1 was confirmed by immunoblotting. In wild-type and unsuccessful transformants, a strong band was detected with Mal d 1-reactive mAb 5H8 at the expected apparent M r of 17 kDa. This band was virtually absent in the transformants that carried the gene-silencing construct. With human IgE antibodies, the same observations were made. CONCLUSIONS: Mal d 1 expression was successfully reduced by RNA interference. This translated into significantly reduced in vivo allergenicity. These observations support the feasibility of the production by gene silencing of apples hypoallergenic for Mal d 1. 相似文献
75.
van Eden W Hauet-Broere F Berlo S Paul L van der Zee R de Kleer I Prakken B Taams L 《International reviews of immunology》2005,24(3-4):181-197
Immunization with microbial or mammalian stress proteins or heat-shock proteins in models of experimental autoimmunity has been observed to lead to increased disease resistance. Furthermore, such immunization has been proposed to result in the induction and expansion of T cells that suppress disease upon transfer. Comparisons of microbial heat-shock proteins with other conserved immunogenic proteins of bacterial origin have indicated a unique capacity for heat-shock proteins to induce a regulatory phenotype in T cells, such as reflected by the production of IL10. Also, studies in children with chronic arthritis have indicated that T-cell responses to heat-shock proteins are associated with a benign course of the disease and with remission. Furthermore, in patients, heat-shock-protein-(HSP-) activated T cells were shown to display regulatory phenotypes consistent with CD4+ CD25+ T regulatory cells. 相似文献
76.
Although many experiments on laboratory stocks ofDrosophila have suggested that mate choice is a major feature of sexual selection in this organism, few attempts have been made to measure its extent in wild populations. In this study, a crossing design was used to obtain a set of 13 genetically identical independent lines representative of genotypes from an African population ofDrosophila melanogaster. They were tested for variation in sexual behavior using dyadic tests. Significant variation in orientation and vibration latencies was found for males, and in mating speed and copulation duration for both sexes. No evidence of assortative mating, either positive or negative, was found. The absence of a correlation in mating speed between males and females sharing the same genotype leads us to doubt the applicability of the notion of male eagerness and female reluctancy inDrosophila and the importance of vigor as a factor in mating speed. The absence of mate choice in natural populations ofDrosophila seems to us the most likely hypothesis on the basis of both theory and empirical evidence. 相似文献
77.
78.
John P. McCarthy Marcas M. Bamman Janice M. Yelle Adrian D. LeBlanc Roger M. Rowe Michael C. Greenisen Stuart M. C. Lee Elisabeth R. Spector Suzanne M. Fortney 《European journal of applied physiology》1997,76(1):32-40
Resistance exercise has been suggested to increase blood volume, increase the sensitivity of the carotid baroreceptor cardiac reflex response (BARO), and decrease leg compliance, all factors that are expected to improve orthostatic tolerance. To further test these hypotheses, cardiovascular responses to standing and to pre-syncopal limited lower body negative pressure (LBNP) were measured in two groups of sedentary men before and after a 12-week period of either exercise (n = 10) or no exercise (control, n = 9). Resistance exercise training consisted of nine isotonic exercises, four sets of each, 3 days per week, stressing all major muscle groups. After exercise training, leg muscle volumes increased (P?0.05) by 4–14%, lean body mass increased (P = 0.00) by 2.0 (0.5)?kg, leg compliance and BARO were not significantly altered, and the maximal LBNP tolerated without pre-syncope was not significantly different. Supine resting heart rate was reduced (P = 0.03) without attenuating the heart rate or blood pressure responses during the stand test or LBNP. Also, blood volume (125I and 51Cr) and red cell mass were increased (P?0.02) by 2.8% and 3.9%, respectively. These findings indicate that intense resistance exercise increases blood volume but does not consistently improve orthostatic tolerance. 相似文献
79.
Differential expression of extracellular matrix metalloproteinase inducer (CD147) in normal and ulcerated corneas: role in epithelio-stromal interactions and matrix metalloproteinase induction 总被引:12,自引:0,他引:12
下载免费PDF全文
![点击此处可从《The American journal of pathology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Gabison EE Mourah S Steinfels E Yan L Hoang-Xuan T Watsky MA De Wever B Calvo F Mauviel A Menashi S 《The American journal of pathology》2005,166(1):209-219
Extracellular matrix metalloproteinase inducer (EMMPRIN) was originally identified on the tumor cell surface as an inducer of matrix metalloproteinase (MMP) production in neighboring fibroblasts. Here we demonstrate a role for EMMPRIN in MMP induction during corneal wound healing. MMP and EMMPRIN expression was analyzed in normal and ulcerated human corneas, as well as in corneal epithelial and stromal cells in culture using confocal microscopy, zymography, immunoblots, and real-time polymerase chain reaction. In normal cornea EMMPRIN was predominantly expressed in the epithelium but was markedly induced in the anterior stroma of ulcerated corneas. This coincided with MMP-2 induction that co-localized with EMMPRIN at the epithelio-stromal boundary. The role of epithelial-stromal interaction in MMP induction was investigated in an in vitro co-culture system and demonstrated an induction and co-localization of EMMPRIN and MMP-2 in the fibroblasts at the interface with epithelial cells. Direct contact of fibroblasts with EMMPRIN-containing purified epithelial cell membranes also induced MMP-1, MMP-2, and EMMPRIN and this was inhibited by a blocking anti-EMMPRIN antibody, suggesting that EMMPRIN was primarily responsible for this induction. These findings, and the up-regulation of EMMPRIN by epidermal growth factor and transforming growth factor-beta, demonstrate a role for EMMPRIN in wound healing and suggest that sustained local up-regulation of EMMPRIN and MMPs in chronic situations in which healing is delayed may lead to excessive matrix degradation and corneal melts. 相似文献
80.
ApoAI deficiency results in marked reductions in plasma cholesterol but no alterations in amyloid-beta pathology in a mouse model of Alzheimer's disease-like cerebral amyloidosis
下载免费PDF全文
![点击此处可从《The American journal of pathology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Fagan AM Christopher E Taylor JW Parsadanian M Spinner M Watson M Fryer JD Wahrle S Bales KR Paul SM Holtzman DM 《The American journal of pathology》2004,165(4):1413-1422
Epidemiological studies suggest links between cholesterol metabolism and Alzheimer's disease (AD), with hypercholesterolemia associated with increased AD risk, and use of cholesterol-lowering drugs associated with decreased risk. Animal models using cholesterol-modifying dietary or pharmacological interventions demonstrate similar findings. Proposed mechanisms include effects of cholesterol on the metabolism of amyloid-beta (Abeta), the protein that deposits in AD brain. To investigate the effect of genetic alterations in plasma cholesterol on Abeta pathology, we crossed the PDAPP transgenic mouse model of AD-like cerebral amyloidosis to apolipoprotein AI-null mice that have markedly reduced plasma cholesterol levels due to a virtual absence of high density lipoproteins, the primary lipoprotein in mice. Interestingly and in contrast to models using non-physiological high fat diets or cholesterol-lowering drugs to modify plasma cholesterol, we observed no differences in Abeta pathology in PDAPP mice of the various apoAI genotypes despite robust differences in plasma cholesterol levels between the groups. Absence of apoAI also resulted in reductions in brain but not cerebrospinal fluid cholesterol, but had no effect on brain apolipoprotein E levels. These and other data suggest that it is perhaps the level of brain apolipoprotein E, not cholesterol per se, that plays a primary role in brain Abeta metabolism. 相似文献