PCR检测角膜内单疱病毒DNA的实验及临床研究

为了探索单疱病毒性角膜炎的发病机制和快速诊断ＨＳＫ。方法应用多聚酶链反应对感染的ＨＳＫ的２０只纯新西兰白兔和６０例ＨＳＫ患者的角膜进行了单疱病毒－１－ＤＮＡ检测。结果急性感染期的５只兔角膜和１２只人角膜均阳性；用稳定期４５ｄ的兔角供体行部分穿透性角膜移植术，术后５０ｄ１０只兔角膜中７只阳性，稳定期６个月－６年的１８只人角膜片，１２只阳性；３０例临床可疑ＨＳＫ角膜刮取物，２６例阳性；５只未感染ＨＳＫ     

Chronic Ethanol Inhibits Inositol Metabolism in Specific Brain Regions

Many neurotransmitters and hormones in the nervous system transmit signals through receptors coupled to the poly-phosphoinositide (PI) signaling pathway. In this study, an in vivo protocol with (³H]inositol was used to examine the effect of chronic ethanol administration on inositol metabolism and poly-PI turnover in the cerebral cortex, hippocampus, and cerebellum of mouse brain. C57BL/6 mice were given a nutritionally complete liquid diet containing either ethanol (5%, w/v) or isocaloric sucrose for 2 months. Mice were injected intracerebrally with ^rH]inositol; after 16 or 24 hr, they were injected intraperitoneally with lithium (8 mEq/kg body weight) to inhibit the inositol monophosphatase (IP₁) activity. All mice were decapitated 4 hr after lithium injection. Labeled inositol phospholipids accounted for 16 to 23% of total labeled inositol in different regions of control mouse brain, and the percentages in the hippocampus were consistently higher than the cerebral cortex and cerebellum. In control mice, the percentages of labeled IP, after a 4-hr lithium treatment were 11.5%, 9.9%, and 3.7% for cerebral cortex, hippocampus, and cerebellum, respectively. Chronic ethanol feeding resulted in a significant (p < 0.05) decrease in the percent of labeled IP₁ and inositol phospholipids, and this effect was observed in the cerebral cortex and, to a lesser extent, hippocampus but not cerebellum. When ratios of labeled IP₁ were expressed against labeled inositol phospholipids as an index of the poly-PI turnover activity, significant decreases in IP/lipid ratios were observed in the cerebral cortex, but not the hippocampus or cerebellum. Although mice killed 24 + 4 hr after the last ethanol feeding would have experienced an 8-hr period of ethanol withdrawal, compared with the 16 + 4-hr group, no differences in IP/lipid ratios were observed between the two time groups. These results illustrate regional differences in the effect of chronic ethanol on inositol metabolism in the brain, but no difference in poly-PI turnover in brain due to ethanol withdrawal.     

选择性髂内动脉结扎与栓塞对盆腔血供的影响

目的：旨在探讨选择性髂内动脉结扎与栓塞两种方法对盆腔血供的影响。方法：将两组犬在膀胱上动脉分支以下之髂内动脉分别行结扎与栓塞，对比观察两组膀胱创面出血、盆腔动脉造影及盆腔脏器组织学变化，以确定其盆腔脏器的血供情况。结果：①结扎组膀胱创面出血明显大于栓塞组；②结扎组髂内动脉侧支循环建立时间明显早于且数目多于栓塞组；③两组盆腔脏器均未见缺血坏死。结论：栓塞组阻断髂内动脉侧支循环的数目多于结扎组，故对盆腔血供的影响大于结扎组。避免髂内动脉阻断范围过广可减少盆腔脏器缺血坏死的机会。     

食管癌高发及低发区患者DNA修复功能的研究

本文对食管癌高、低发区食管癌患者和正常人,经MNNG诱导的DNA损伤修复功能进行了研究。实验采用外周血淋巴细胞培养的方法。每组又分为对照组及MNNG诱导组。样品以~3H-TdR标记后经液闪仪计数法进行检测。结果发现,低发区正常人经MNNG诱导的DNA损伤修复功能明显高于对照组,两个地区食管癌患者修复能力均低于正常人,与地区无关。     

The correlation of ureaplasma urealyticum infection with infertility

Summary. A prevalence study of Ureaplasma urealyticum (UU) infection of the male genital tract was carried out in Shanghai between March 1992 and June 1995. Significantly higher frequency of UU infection was found among infertile males (549/1416) as compared to fertile controls (34/375). Examination of 8 specimens each from infertile men and fertile subjects by electron microscopy, immunogold and immunofluorescence techniques, demonstrated adhesion of Ureaplasma urealyticum to the membrane of spermatozoa and exfoliated germ cells. In addition, gold particles on Ureaplasma urealyticum were found to be adhered to the sperm surface in 4 of the 8 samples. Strong specific anti-UU fluorescence was detected in 6 of 8 samples, mainly on the midpieces and post-acrosomal regions of the spermatozoa.
To further study the effects of Ureaplasma urealyticum on fertility, 47 male Sprague-Dawley (SD) rats were infected artificially with Ureaplasma urealyticum serotype 8 (T960). Morphological changes in the seminiferous tubules were observed 3–5 weeks after inoculation in the sacrificed animals. Dramatic impairment of spermatogenesis of both testes was found in 11 rats. Mating experiment confirmed infertility in 12 of 40 rats. Offsprings of the infected rats were significantly smaller than those of controls in terms of prenatal weights and birthweights.     

Histopathologic findings in a case of recurrent parotitis in adulthood

Most cases with recurrent parotitis in childhood (RPC) can be cured before puberty.^1–3 A few of them, however, may continue to adulthood.^1–4 To distinguish this condition from other types of chronic parotitis in adults, such as chronic obstructive parotitis,⁵ we suggest that the diagnosis of recurrent parotitis in adults (RPA) be reserved for the cases derived from RPC.⁶ The pathophysiology and development of RPA is still not well known. To our knowledge, there are few reports of RPA in the literature, especially concerning its histopathology. This report describes a cases of RPA studied with sialography and light and electron microscopy.     

葡萄糖酸锌糖浆的制备与含量测定方法

本文报告了葡萄糖酸锌糖浆的制法及用二甲橙指示剂法测定含量的方法。结果表明制法简便,含量测定方法准确。     

深低温冷冻保存家兔性腺器官的研究

自１９９２年７月开始行深低温（－１９６℃）冷冻保存兔性腺研究。冷冻睾丸３６个，１５天后解冻，完好率９１．７％，其中２０个行器官移植，睾丸接通血管均即时建立血循环。４只兔的冷冻睾丸自体移植后，每次采精７４０．６±１８９０．４万个。２只兔的冷冻睾丸异体移植后，每次采精６３．４±３７．７万个。冷冻兔卵巢１４个，１５天后解冻，完好率９２．９％，其中８个行器官移植，卵巢接通血管均即时建立血循环，术后雌二醇水平为２．７２～１２７．３ｐｍｏｌ／Ｌ。冷冻睾丸及卵巢移植一段时间后病理切片证实睾丸及卵巢组织存活。