AngⅡ受体拮抗剂对SHR肾小球系膜细胞增殖作用

目的观察血管紧张素Ⅱ1型受体（AT1受体）拮抗剂Losartan（芦沙坦）对自发性高血压大鼠（SHR）肾小球系膜细胞增殖的作用。方法采用细胞培养和3H-胸腺嘧啶核苷（3H－TdR）掺入技术。结果1．SHR系膜细胞血管紧张素Ⅱ（AngⅡ）组的3H－TdR掺入率高于对照组;2．SHR系膜细胞AngⅡ＋Losar－tan组的3H－TdR掺入率低于AngⅡ组;3．AngⅡ对SHR肾小球系膜细胞增殖的刺激作用和Losartan的抑制作用均呈剂量依赖关系;4．AngⅡ对正常血压大鼠系膜细胞的3H－TdR无影响。结论Losartan能逆转AugⅡ对SHR肾小球系膜细胞的增殖作用,从细胞水平体现其肾脏保护作用。     

Climate, traffic-related air pollutants, and asthma prevalence in middle-school children in taiwan

This study compared the prevalence of asthma with climate and air pollutant data to determine the relationship between asthma prevalence and these factors. We conducted a nationwide survey of respiratory illness and symptoms in middle-school students in Taiwan. Lifetime prevalences of physician-diagnosed asthma and of typical symptoms of asthma were compared to air monitoring station data for temperature, relative humidity, sulfur dioxide, nitrogen oxides, ozone, carbon monoxide, and particulate matter with aerodynamic diameter [less than/equal to] 10 microm (PM(10)). A total of 331,686 nonsmoking children attended schools located within 2 km of 55 stations. Asthma prevalence rates adjusted for age, history of atopic eczema, and parental education were associated with nonsummer (June-August) temperature, winter (January-March) humidity, and traffic-related air pollution, especially carbon monoxide and nitrogen oxides, for both girls and boys. Nonsummer temperature, winter humidity, and traffic-related air pollution, especially carbon monoxide and nitrogen oxides, were positively associated with the prevalence of asthma in middle-school students in Taiwan.     

Patient Compliance and its Impact on Treatment Outcomes

Despite an abundance of literature that assesses medication compliance associated with specific diseases, its impact on patient outcomes remains poorly characterised, perhaps due to the complexity associated with its influence and measurement. Much of the previous research includes end-points that were reflective of the duration and pattern of medication use; the value of which is questionable. In fact, the accuracy of compliance data remains highly controversial due to the difficulties associated with its measurement. Monitoring, by itself, may result in minor improvements in medication compliance; monitoring that is coupled to a meaningful clinical outcome that patients can self-measure results in enhanced compliance.Disease management programmes frequently contain interventions to impact compliance. Disease management programme developers interested in positively affecting compliance should incorporate patient self-monitoring methods into their compliance interventions.     

依拉地平治疗原发性高血压

本报告了依拉地平（isradipine）治疗30例原发性高血压的临床效果及安全性。观察期限6周,结果显示依拉地平1.25mg,每日2次,有14例病人获得满意的疗效,卧位血压由165.5±3.2/100.9±2.9mmHg降至146.1±1.2/89.1±2.5mmHg。11例病人服用剂量增至2.5mg,每日2次,卧位血压降至148.6±4.1/91.5±2.2mmHg。3例加服马来酸依那普利10mg,每日2次,血压降至152.5±2.3/94.9±5.6mmHg。2例病人降压效果不明显。单用依拉地平治疗高血压总有效率为83.3%,副作用较少,并随用药时间的延长而消失。     

173例胃癌临床分析

目的：提高早期胃癌的诊断水平,防止误诊。方法：采用X线钡餐造影及纤维胃镜等检查手段等。结果：本组173例胃癌中,胃癌好发部位贲门部79例,胃窦部37例;男性以分化型癌占优势,且随年龄的增长,所占比率升高等。结论：对贲门及胃窦溃疡病变应进行追踪寻访,对已达到重度不典型增生或可疑癌者应劝其早日施行手术治疗,对不典型胃癌如腰背困痛、腹腔淋巴结肿大、腹部肿块、胃溃疡术后又出现反复腹痛、慢性萎缩型胃部病变及胃粘膜的肠上皮化生,应警惕胃癌的发生。     

端头修饰PEO有序膜的制备及其微摩擦性能的初步研究

以对氨基苯磺酸修饰聚乙二醇端头,制备兼具刚性与极性端头的聚合物,考察其制备ＬＢ膜与自组装膜的条件,用原子力／摩擦力显微镜表征自组装膜的表面形貌,对其微摩擦性能进行初步的探讨。     

滇白珠化学成分的研究(Ⅱ)

从滇白珠Ｇａｎｌｔｈｅｒｉａ ｙｕｎｎａｎｅｎｓｉｓ根的乙酸忆是活性部位分得１０个化合物,经理化性质和波谱分析,它们的结构分别鉴定为阿魏酸（Ⅰ）,氯原酸（Ⅱ）,（＋）－儿茶素（Ⅲ）,原花色素Ａ２,芦丁,槲皮素,水杨酸,香草酸,２,５－二羟基七甲酸和原儿茶酸,其中Ⅳ为杜鹃化科植物中首次报道,其它９个化合物为白珠树属植物中首次分得。     

The experimental study on mixed culture of osteoblasts and tricalcium phosphate ceramicsin vitro

Summary To study the effects of tricalcium phosphate (TCP) ceramics on osteoblasts, the rat osteoblasts were cultured with the TCP ceramicsin vitro. Scanning electron microscopy and the colorimetric methyl-thiazol-tetrazolium assay showed that the osteoblasts could adhere well to the surface of the ceramics and the culture dish, and the proliferation of the cells was not inhibited. The results demonstrated that TCP ceramics possessed an excellent cytocompatibility with the osteoblasts, and had some promoting effects on proliferation of osteoblasts. This project was supported by grant from the National Natural Science Foundation of China (No. 59493202).     

王自敏治疗早、中期慢性肾功能衰竭的经验

慢性肾功能衰竭一旦进入终末尿毒症期,临床仅能依靠透析和肾移植,别无良策,但由于其造价昂贵,尚难普及。因此,对于其早、中期的治疗则更显重要。王自敏教授从事中医肾病临床数十年,积累了丰富的经验,兹总结如下。１抓住病机正中肯綮慢性肾功能衰竭是各种慢性肾脏疾...     

大肠癌和腺瘤细胞凋亡的特征和意义

目的　观察大肠癌和腺瘤组织细胞凋亡的特征,探讨细胞凋亡和增殖在大肠癌和腺瘤组织中的意义。方法　采用脱氧核糖核酸末端转移酶介导的ｄＵＴＰ缺口末端标记（ＴＵＮＥＬ）法检测凋亡细胞,以凋亡细胞百分率作为凋亡指数（Ａｐｏｐｔｉｃｉｎｄｅｘ,ＡＩ）对１５例大肠癌、１０例腺瘤、５例正常大肠粘膜进行凋亡细胞原位观察。结果　ＴＵＮＥＬ阳性物质位于细胞核内,常聚集于核膜附近,呈新月状和块状。大肠正常粘膜ＴＵＮＥＬ阳性细胞全部位于表层上皮中,数量很少（ＡＩ＝１．７４±１．０１）,腺瘤组织中ＴＵＮＥＬ阳性细胞相对较多（ＡＩ＝３３．４６±１１．３９）,大肠癌组织ＴＵＮＥＬ阳性细胞散在分布,与腺瘤相比数量明显减少（ＡＩ＝１１．０８±４．２７）（Ｐ＜０．０１）。结合增殖细胞核抗原（ＰＣＮＡ）实验结果可见,腺瘤组织中ＡＩ,ＰＩ（ＰＩ＝２７．２４±７．６６）均高,而大肠癌组织中则以细胞增殖（ＰＩ＝５４．１８±１０．４１）占优势。结论　大肠肿瘤癌变过程中不仅存在活跃的细胞增殖,而且有大量的细胞凋亡,因而增加了粘膜上皮的不稳定性,高的增殖能力通过选择而占优势,从而导致癌变的发生。