全文获取类型
收费全文 | 1159篇 |
免费 | 104篇 |
专业分类
耳鼻咽喉 | 4篇 |
儿科学 | 32篇 |
妇产科学 | 58篇 |
基础医学 | 147篇 |
口腔科学 | 24篇 |
临床医学 | 178篇 |
内科学 | 172篇 |
皮肤病学 | 13篇 |
神经病学 | 157篇 |
特种医学 | 26篇 |
外科学 | 129篇 |
综合类 | 9篇 |
一般理论 | 1篇 |
预防医学 | 148篇 |
眼科学 | 29篇 |
药学 | 47篇 |
中国医学 | 1篇 |
肿瘤学 | 88篇 |
出版年
2024年 | 3篇 |
2023年 | 16篇 |
2022年 | 26篇 |
2021年 | 45篇 |
2020年 | 34篇 |
2019年 | 67篇 |
2018年 | 65篇 |
2017年 | 42篇 |
2016年 | 42篇 |
2015年 | 40篇 |
2014年 | 64篇 |
2013年 | 74篇 |
2012年 | 101篇 |
2011年 | 101篇 |
2010年 | 45篇 |
2009年 | 49篇 |
2008年 | 66篇 |
2007年 | 66篇 |
2006年 | 72篇 |
2005年 | 63篇 |
2004年 | 46篇 |
2003年 | 45篇 |
2002年 | 45篇 |
2001年 | 1篇 |
2000年 | 2篇 |
1999年 | 7篇 |
1998年 | 6篇 |
1997年 | 5篇 |
1996年 | 2篇 |
1995年 | 2篇 |
1994年 | 1篇 |
1993年 | 3篇 |
1992年 | 1篇 |
1990年 | 3篇 |
1989年 | 1篇 |
1988年 | 2篇 |
1987年 | 2篇 |
1985年 | 1篇 |
1980年 | 1篇 |
1979年 | 1篇 |
1974年 | 1篇 |
1973年 | 4篇 |
排序方式: 共有1263条查询结果,搜索用时 15 毫秒
21.
Fibroblast growth factor-23 mutants causing familial tumoral calcinosis are differentially processed
Larsson T Davis SI Garringer HJ Mooney SD Draman MS Cullen MJ White KE 《Endocrinology》2005,146(9):3883-3891
Familial tumoral calcinosis (TC, OMIM 211900) is a heritable disorder characterized by hyperphosphatemia, normal or elevated serum 1,25-dihydroxyvitamin D, and often severe ectopic calcifications. Two recessive mutations in fibroblast growth factor-23 (FGF23), serine 71/glycine (S71G) and serine 129/phenylalanine (S129F), were identified as causing TC. Herein, we undertook comprehensive biochemical analyses of an extended TC family carrying the S71G FGF23 mutation, which revealed that heterozygous (serine/glycine, S/G) individuals had elevated serum FGF23 C-terminal fragments compared with wild-type (serine/serine, S/S) family members (P < 0.025). To understand the differential processing of FGF23 in TC patients, we transiently expressed S71G as well as S129F FGF23. FGF23 ELISA in tandem with Western analyses revealed increased proteolytic cleavage of mutant FGF23 and a limited secretion of intact protein. Furthermore, S71G and S129F FGF23 carrying mutations that disrupt the furin-like protease RXXR motif in FGF23 rescued the secretion of the intact protein, and both TC mutant proteins harboring the R176Q mutation revealed no altered sensitivity to trypsin compared with the native (R176Q)FGF23. Finally, S71G, but not S129F mutant FGF23, is rescued by temperature. In summary, FGF23 mutations causing TC lead to increased intracellular proteolysis of FGF23, most likely by furin-like proteases, due to conformational changes of the mutant protein. The destabilizing nature of these mutations provides new insight into the pathophysiology of TC and exemplifies the physiological importance of FGF23 in phosphate and vitamin D metabolism. 相似文献
22.
Larsson T Yu X Davis SI Draman MS Mooney SD Cullen MJ White KE 《The Journal of clinical endocrinology and metabolism》2005,90(4):2424-2427
Gain-of-function mutations in fibroblast growth factor-23 (FGF23) are responsible for autosomal dominant hypophosphatemic rickets, a disorder of isolated renal phosphate wasting. Patients with the disorder display hypophosphatemia with normocalcemia as well as inappropriately normal 1,25-dihydroxyvitamin D [1,25(OH)2D3] concentrations. Reciprocally tumoral calcinosis (TC) patients are often hyperphosphatemic with inappropriately normal or elevated serum 1,25(OH)2D3 levels and have ectopic and vascular calcifications, a phenotype similar to that of Fgf23 null mice. Therefore, the goal of the present studies was to test whether FGF23 was a candidate gene for TC. Two sisters in a consanguineous TC family had hyperphosphatemia and normal 1,25(OH)2D3 levels with characteristic ectopic and vascular calcifications. Interestingly, these patients had low-normal intact serum FGF23 levels but demonstrated FGF23 concentrations approximately 40 times normal when assessed with a C-terminal FGF23 serum assay. Mutational analyses identified a homozygous S71G mutation in FGF23 in the TC patients, which was not found in control alleles. Finally, modeling demonstrated that the S71G mutation most likely destabilizes full-length FGF23. In summary, recessive FGF23 mutations can lead to TC. Additionally, our findings indicate that FGF23 may adopt an unstable conformation in some TC patients, possibly leading to nonfunctional FGF23 protein. 相似文献
23.
Siobhan O. Burns Vincent Plagnol Beatriz Morillo Gutierrez Daifulah Al Zahrani James Curtis Miguel Gaspar Amel Hassan Alison M. Jones Marian Malone Dyanne Rampling Alex McLatchie Rainer Doffinger Kimberly C. Gilmour Frances Henriquez Adrian J. Thrasher H. Bobby Gaspar Sergey Nejentsev 《The Journal of allergy and clinical immunology》2014
24.
25.
26.
27.
28.
Sonia Jean PhD Siobhan O'Donnell Claudia Lagacé Peter Walsh Christina Bancej Jacques P Brown Suzanne Morin Alexandra Papaioannou Susan B Jaglal William D Leslie for the Osteoporosis Surveillance Expert Working Group 《Journal of bone and mineral research》2013,28(6):1283-1289
Age‐standardized rates of hip fracture in Canada declined during the period 1985 to 2005. We investigated whether this incidence pattern is explained by period effects, cohort effects, or both. All hospitalizations during the study period with primary diagnosis of hip fracture were identified. Age‐ and sex‐specific hip fracture rates were calculated for nineteen 5‐year age groups and four 5‐year calendar periods, resulting in 20 birth cohorts. The effect of age, calendar period, and birth cohort on hip fracture rates was assessed using age‐period‐cohort models as proposed by Clayton and Schiffers. From 1985 to 2005, a total of 570,872 hospitalizations for hip fracture were identified. Age‐standardized rates for hip fracture have progressively declined for females and males. The annual linear decrease in rates per 5‐year period were 12% for females and 7% for males (both p < 0.0001). Significant birth cohort effects were also observed for both sexes (p < 0.0001). Cohorts born before 1950 had a higher risk of hip fracture, whereas those born after 1954 had a lower risk. After adjusting for age and constant annual linear change (drift term common to both period and cohort effects), we observed a significant nonlinear birth cohort effect for males (p = 0.0126) but not for females (p = 0.9960). In contrast, the nonlinear period effect, after adjustment for age and drift term, was significant for females (p = 0.0373) but not for males (p = 0.2515). For males, we observed no additional nonlinear period effect after adjusting for age and birth cohort, whereas for females, we observed no additional nonlinear birth cohort effect after adjusting for age and period. Although hip fracture rates decreased in both sexes, different factors may explain these changes. In addition to the constant annual linear decrease, nonlinear birth cohort effects were identified for males, and calendar period effects were identified for females as possible explanations. 相似文献
29.
Trevor A. Harley Lesley J. Jessiman Siobhan B. G. MacAndrew Arlene Astell 《Aphasiology》2013,27(3):321-335
Abstract This paper will address the current practice of speech therapists setting homework for aphasic patients. 相似文献
30.
Matthew D Walker Katherine Dinelle Rick Kornelsen Siobhan McCormick Chenoa Mah James E Holden Matthew J Farrer A Jon Stoessl Vesna Sossi 《Journal of cerebral blood flow and metabolism》2013,33(1):59-66
Longitudinal measurements of dopamine (DA) uptake and turnover in transgenic rodents may be critical when developing disease-modifying therapies for Parkinson''s disease (PD). We demonstrate methodology for such measurements using [18F]fluoro-3,4-dihydroxyphenyl-L-alanine ([18F]FDOPA) positron emission tomography (PET). The method was applied to 6-hydroxydopamine lesioned rats, providing the first PET-derived estimates of DA turnover for this species. Control (n=4) and unilaterally lesioned (n=11) rats were imaged multiple times. Kinetic modeling was performed using extended Patlak, incorporating a kloss term for metabolite washout, and modified Logan methods. Dopaminergic terminal loss was measured via [11C]-(+)-dihydrotetrabenazine (DTBZ) PET. Clear striatal [18F]FDOPA uptake was observed. In the lesioned striatum the effective DA turnover increased, shown by a reduced effective distribution volume ratio (EDVR) for [18F]FDOPA. Effective distribution volume ratio correlated (r>0.9) with the [11C]DTBZ binding potential (BPND). The uptake and trapping rate (kref) decreased after lesioning, but relatively less so than [11C]DTBZ BPND. For normal controls, striatal estimates were kref=0.037±0.005 per minute, EDVR=1.07±0.22 and kloss=0.024±0.003 per minute (30 minutes turnover half-time), with repeatability (coefficient of variation) ≤11%. [18F]fluoro-3,4-dihydroxyphenyl-L-alanine PET enables measurements of DA turnover in the rat, which is useful for developing novel therapies for PD. 相似文献