系统免疫炎症指数在胰腺导管腺癌患者Whipple术后总体生存率中的预测价值

目的:研究系统免疫炎症指数(SII)在胰腺导管腺癌患者Whipple术后总体生存率中的预测价值。方法:回顾性分析2010年1月1日至2017年12月31日在郑州大学第一附属医院进行Whipple术的胰腺导管腺癌患者临床资料,计算SII值。取SII最佳截断值为900,以900为界值将所有患者分为低SII组(SII≤900...     

Combined prognostic value of preoperative serum thyrotrophin and thyroid hormone concentration in papillary thyroid cancer

BackgroundA growing number of studies have found a close association between thyroid hormones and thyrotrophin (TSH), and they also have prognostic significance in some cancer types; this study aimed to investigate the prognostic value of free triiodothyronine (fT3), free thyroxine (fT4), fT3/fT4, TSH, and their combination in patients with papillary thyroid carcinoma (PTC).MethodsThis study retrospectively analyzed the relevant data of 726 newly diagnosed PTC patients. Both univariate and multivariate analyses were used to predict the recurrence rate, and a risk score was established. In addition, with the use of a random survival forest, a random forest (RF) score was constructed. After calculating the area under the curve (AUC), the diagnostic efficacy of risk score, RF score, and four indicators was compared.ResultsfT3, fT4, fT3/fT4, and TSH were strongly associated with some invasive clinicopathological features and postoperative recurrence. Patients with high expression of fT4 and TSH have a high risk of recurrence. By contrast, patients with high expression of fT3 and fT3/fT4 have a low risk of recurrence. At the same time, the combined use of various indicators is more helpful for establishing an accurate diagnosis. By comparison, we found that the RF score was better than the risk score in terms of predicting the recurrence of PTC.ConclusionThe diagnostic accuracy of a combination of fT3, fT4, fT3/fT4, and TSH can help improve our clinical estimate of the risk of recurrent PTC, thus allowing the development of a more effective treatment plan for patients.     

The role of hypoxia inducible factor-1α in the increased MMP-2 and MMP-9 production by human monocytes exposed to nickel nanoparticles

Nickel is an important economic commodity, but it can cause skin sensitization and may cause lung diseases such as lung fibrosis, pneumonitis, bronchial asthma and lung cancer. With development of nanotechnology, nano-sized nickel (Nano-Ni) and nano-sized titanium dioxide (Nano-TiO?) particles have been developed and produced for many years with new formulations and surface properties to meet novel demands. Our previous studies have shown that Nano-Ni instilled into rat lungs caused a greater inflammatory response as compared with standard-sized nickel (5 μm) at equivalent mass concentrations. Nano-Ni caused a persistent high level of inflammation in lungs even at low doses. Recently, several studies have shown that nanoparticles can translocate from the lungs to the circulatory system. To evaluate the potential systemic effects of metal nanoparticles, we compared the effects of Nano-Ni and Nano-TiO? on matrix metalloproteinases 2 and 9 (MMP-2 and MMP-9) gene expression and activity. Our results showed that exposure of human monocyte U937 to Nano-Ni caused dose- and time- dependent increase in MMP-2 and MMP-9 mRNA expression and pro-MMP-2 and pro-MMP-9 activity, but Nano-TiO? did not. Nano-Ni also caused dose- and time- related increase in tissue inhibitor of metalloproteinases 1 (TIMP-1), but Nano-TiO? did not. To determine the potential mechanisms involved, we measured the expression of hypoxia inducible factor 1α (HIF-1α) in U937 cells exposed to Nano-Ni and Nano-TiO?. Our results showed that exposure to Nano-Ni caused HIF-1α accumulation in the nucleus. Furthermore, pre-treatment of U937 cells with heat shock protein 90 (Hsp90) inhibitor, 17-(Allylamino)-17-demethoxygeldanamycin (17-AAG), prior to exposure to Nano-Ni significantly abolished Nano-Ni-induced MMP-2 and MMP-9 mRNA upregulation and increased pro-MMP-2 and pro-MMP-9 activity. Our results suggest that HIF-1α accumulation may be involved in the increased MMP-2 and MMP-9 production in U937 cells exposed to Nano-Ni.     

复方鱼腥草片微生物限度检查方法的探讨

目的:建立适合复方鱼腥草片的微生物限度检查方法,促进以品种确定方法工作的开展.方法:对由6个生产企业提供的涉及常规法、培养基稀释法、离心沉淀集菌法的复方鱼腥草片细菌计数检查方法,按<中国药典>2005年版一部附录的要求进行再验证.结果:验证数据分析结果表明,6个生产企业的样品采用培养基稀释法进行细菌计数方法检查,试验菌回收率均达到要求.结论:可将复方鱼腥草片的微生物限度细菌计数检查方法统一为培养基稀释法.     

冠心病患者的血浆BNP水平分析

目的探讨冠心病患者血浆脑钠肽(BNP)浓度的变化及其临床意义。方法入选住院AMI患者30例、UAP患者30例及对照组30例,测定BNP浓度,同时测定磷酸肌酸激酶同工酶(CK-MB),进行比较分析及相关分析。结果冠心病患者血浆BNP水平显著高于对照组(P<0.05),急性心肌梗死组BNP水平高于不稳定型心绞痛组(P<0.05),急性心梗及不稳定型心绞痛患者的BNP与其他指标进行相关性分析,结果表明,BNP与CK-MB之间有良好的正相关(P<0.05)。结论冠心病患者血浆BNP水平明显升高,在急性心肌梗死和不稳定型心绞痛患者中两者有明显的相关性,提示血浆BNP在冠心病发病机制中起重要作用,对疾病的危险分层和预后判断具有重要的临床价值。     

高效液相色谱法测定翁沥通胶囊中毛蕊异黄酮葡萄糖苷含量

目的 建立翁沥通胶囊中毛蕊异黄酮葡萄糖苷的含量测定方法.方法 采用高效液相色谱法,色谱柱为Waters XTERRA MS C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-1.5%磷酸(20:80),检测波长为260 nm,流速为1.0 mL/min,柱温为30℃.结果 毛蕊异黄酮葡萄糖苷的线性范围为7.55～151.1 mg/L(r=0.999 2),平均回收率为98.17%,RSD%=1.01%(n=9).结论 该方法简便、快速、专属性强,可有效控制翁沥通胶囊中毛蕊异黄酮葡萄糖苷的含量,能为生产及流通控制翁沥通胶囊质量提供参考.     

水溶性槲皮素的研究

水溶性槲皮素的研究佘戟１）莫丽儿康铁邦梁念慈（广东医学院化学教研室、医用生物化学研究所，湛江５２４０２３）槲皮素（ｑｕｅｒｃｅｔｉｎ；３，３′，４′，５，７－ｐｅｎｔａｈｙｄｒｏｘｙｆｌａｖｏｎｅ）存在于许多植物体内，它的提取和纯化已有大量的报道．国...     

耳鸣掩蔽治疗条件的测定

目的 寻找耳鸣掩蔽治疗较为合适的掩蔽时间、掩蔽声类型的组合。方法 受试的２７名耳蜗性耳鸣患者,平均分别来自于三个治疗点,并定为甲、乙、丙三组。按析因试验设计原理将掩蔽声设定为窄带噪声（ｎａｒ－ｒｏｗ ｂａｎｄ ｎｏｉｓｅ,ＮＢＮ）、宽带噪声（ｗｉｄｅ ｂａｎｄ ｎｏｉｓｅ,ＷＢＮ）和言语噪声（ｓｐｅｃｈ ｎｏｉｓｒｅ,ＳＮ）三个因素,掩蔽时间设定为１５、３０、４５ｍｉｎ三个水平共九种组合,每组内患     

蛋白磷酸酶2A的癌性抑制因子表达对喉癌细胞株Hep-2增殖的影响及相关机制研究

目的:研究蛋白磷酸酶2A的癌性抑制因子(cancerous inhibitor of protein phosphatase 2A,CIP2A)表达对喉癌细胞株Hep-2增殖的影响及相关分子机制。方法:以特异性si RNA和阴性对照序列转染喉癌Hep-2细胞,实验分为CIP2A si RNA组与Control si RNA组。平板克隆形成实验检测CIP2A在喉癌细胞增殖中的作用;MTT法检测CIP2A对细胞生长曲线及顺铂敏感性影响;流式细胞术检测细胞周期分布和凋亡率的改变。real-time PCR检测CIP2A m RNA表达水平,Western blot检测CIP2A及生长相关蛋白c-myc、AKT、p-AKT、cyclin D1的蛋白表达水平。结果:沉默CIP2A后,CIP2A在基因及蛋白水平的表达量明显降低,Hep-2细胞生长减缓,克隆的形成被抑制,Control si RNA组为339.00±34.00,CIP2A si RNA组为126.00±33.00,差异有统计学意义(t=-7.796,P=0.010);沉默CIP2A可增加喉癌细胞对顺铂的敏感性,Control si RNA组IC50为(6.67±0.54)μg/ml,CIP2A si RNA组为(3.53±0.32)μg/ml,差异有统计学意义(t=8.598,P=0.001);细胞周期阻滞于G1期,Control si RNA组G1期细胞比例为66.860±1.972,CIP2A si RNA组为74.613±3.357,差异有统计学意义(t=3.449,P=0.026);S期细胞比例减少,Control si RNA组为23.713±1.564,CIP2A si RNA组为17.747±1.255,差异有统计学意义(t=-5.153,P=0.007);沉默CIP2A没有引起细胞凋亡率的变化(P=0.216)。Western blot结果显示,沉默CIP2A表达可下调Cyclin D1(t=-4.531,P=0.011)、c-myc(t=-4.522,P=0.011)、pAKT(t=-4.574,P=0.010)的表达,但对总AKT蛋白的表达没有影响(t=0.051,P=0.962)。结论:沉默CIP2A基因可抑制喉癌Hep-2细胞生长,增加喉癌细胞对顺铂的敏感性,其机制可能与下调c-myc、p-AKT、cyclin D1的表达有关。     

阿魏酸钠对大鼠角膜新生血管的抑制作用研究

目的 探讨阿魏酸钠(SF)对角膜新生血管(CNV)的抑制作用及其机制.方法 将不同质量浓度的SF用于缝线诱导的大鼠CNV模型,观察其抑制CNV形成的效果,采用免疫组织化学法和RT-PCR法检测血管内皮生长因子(VEGF)蛋白和mRNA在角膜组织中的表达水平.结果 0.025%和0.1%SF治疗组CNV面积均小于阴性对照组,以后者更明显,差异均有统计学意义(P＜0.05),0.1%SF治疗组与阳性对照组间差异无统计学意义(P＞0.05);免疫组织化学显示VEGF在正常角膜不表达或在上皮基底细胞有弱表达,阴性对照组染色明显增强,0.1%SF治疗组表达明显减弱;VEGFmRNA和蛋白水平表达有一致性.结论 SF滴眼液能有效地抑制CNV的生长,其抑制VEGF的表达可能是SF抑制CNV生成的一个重要机制.