苯妥英钠对妊娠小鼠的致畸作用及叶酸的干预影响分析

目的:观察苯妥英钠对妊娠小鼠的不良作用,探讨叶酸对其不良效应的干预效果。方法:将100只雌性和50只雄性小鼠随机分为5组(雌雄比例2:1),分别为:①空白对照组(灌胃蒸馏水,10 ml/kg);②苯妥英钠单次剂量组(50mg/kg);③苯妥英钠二次给药组(上、下午各为25 mg/kg);④苯妥英钠单次剂量与叶酸联合用药组(50 mg/kg+0.07 mg/kg);⑤苯妥英钠二次给药与叶酸联合用药组(25 mg/kg+0.035 mg/kg)。观察小鼠是否受孕、流产、畸形、死胎、每窝仔鼠的数目及孕期增重情况等指标。结果:与其他各组相比,单剂量组小鼠的受孕率下降,孕鼠的流产率、死胎率和畸胎率均上升,孕期增重和每窝正常仔鼠数目减少(P<0.05)。与二次给药组和二次给药与叶酸组相比,单次剂量组和单次剂量与叶酸组小鼠的受孕率下降、死胎率上升(P<0.05);单次剂量与叶酸组和二次给药与叶酸组相比,单次剂量组和二次给药组小鼠的受孕率下降、死胎率上升、孕期增重减少(P<0.05)。结论:苯妥英钠可导致妊娠小鼠妊娠意外的增多,小剂量多次给药以及补充叶酸可降低苯妥英钠的不良作用。     

肥胖孕妇妊娠结局的临床分析

目的:探讨孕妇肥胖对分娩方式及围产儿结局的影响。方法:选取2004年1月~2005年1月在华北煤炭医学院附属医院进行产前检查并分娩的单胎初产妇224例,根据孕妇孕前体重指数(BMI)及孕期体重增加量分为3组,孕前BMI≥25者36例为孕前肥胖组;孕前BMI在17~24之间,孕期体重增加≥15 kg者150例为孕期体重过度增加组;孕前BMI在17~24之间,孕期体重增加<15 kg者38例为正常组。探讨各组的剖宫产率、巨大儿率及出现产科合并症的情况。结果:孕前肥胖组及孕期体重过度增加组孕妇的剖宫产率、巨大儿率明显高于正常组(P<0.05)。结论:加强围产期保健,避免肥胖,可减少母婴并发症。     

DNA repair gene ERCC1 and XPD polymorphisms predict glioma susceptibility and prognosis

Aims: We conducted a case-control study in a Chinese population to clarify the association betweenpolymorphisms in ERCC1 and XPD and susceptibility and survival of glioma. Methods: A total of 393 cases and410 controls were selected from March 2007 to December 2011. Genotyping of ERCC1 and XPD was conductedby TaqMan assays using the ABI Prism 7911HT Sequence Detection System. All analyses were performed usingthe STATA statistical package. Results: Polymorphisms in ERCC1 118C/T, ERCC1 8092C/A and XPD Asp312Asnshowed no statistically significant difference between glioma cases and controls. However, individuals with theXPD 751Gln/Gln genotype had an increased risk of developing glioma compared with those with the Lys/Lysgenotype (adjusted OR=1.64, 95% CI: 1.06-2.89). The ERCC1 118T/T genotype was associated with significantlyhigher median survival than the ERCC1 C/C genotype (HR=0.67, 95%CI=0.35-0.96). In addition, individualswith XPD 751Gln/Gln had a lower median survival time than XPD Lys/Lys carriers (HR=0.54, 95%CI=0.37-0.93). Conclusion: In conclusion, we observed that the XPD 751Gln/Gln genotype is associated with gliomasusceptibility, and ERCC1 118 T/T and XPD 751Gln/Gln genotypes confer a significantly better prognosis.     

巨噬细胞修饰低密度脂蛋白对细胞受体活性的影响及前列腺素E_2的作用

为研究低密度脂蛋白经细胞修饰后对细胞受体的影响及前列腺素E2的作用，利用免疫细胞化学和生物亲和素-酶联免疫吸附法分别检测小鼠腹腔巨噬细胞清道夫受体、小鼠皮肤纤维母细胞低密度脂蛋白受体的结合活性，结果发现修饰组的巨噬细胞胞浆及胞膜有强阳性黄褐色颗粒，而药物组仅中度着色；修饰组低密度脂蛋白与其受体结合量（54±13μg／g细胞蛋白）较对照组（144±μg／g细胞蛋白）显著下降（P＜0．01）；药物组受体结合量（100±11μg／g细胞蛋白）较修饰组明显提高（P＜0．05）。表明细胞修饰低密度脂蛋白能够刺激清道夫受体活性，抑制低密度脂蛋白受体活性；前列腺素E2（20mg／L）可对抗细胞修饰低密度脂蛋白对此二种受体的作用。     

保健灸法对更年期大鼠抗氧化能力及脑组织中p53蛋白表达的影响

目的观察保健灸法对更年期大鼠抗氧化能力及脑组织中p53蛋白表达的影响,以探讨保健灸法抗氧化及细胞凋亡的作用。方法SD雌性大鼠42只,其中青年组14只,12月龄28只,随机分为对照组和保健灸组。实验两个月后采用分光光度法检测各组大鼠血清中丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性;采用免疫组化法检测脑组织中p53蛋白的表达水平。结果与青年组比较,更年期对照组大鼠血清中丙二醛(MDA)含量增多,超氧化物歧化酶(SOD)活性下降,脑组织中p53蛋白表达水平明显升高。与更年期对照组比较,更年期保健灸组丙二醛(MDA)含量降低,而超氧化物歧化酶(SOD)活性升高(P<0.05)。脑组织p53蛋白表达水平明显的下降。结论保健灸法具有抗氧化的功能,并且可通过调控p53蛋白的表达,抑制细胞的凋亡,延缓衰老。     

携带肝细胞生长因子基因的减毒沙门菌促血管形成的实验研究

目的探讨携带肝细胞生长因子(HGF)基因的减毒沙门菌体外促血管形成的效应。方法构建携带HGF基因真核表达载体的减毒沙门菌菌株(TPH),体外转染人脐静脉内皮细胞(HUVEC)后观察目的基因表达及其增殖活性;评价TPH的细胞表达上清对鸡胚绒膜尿囊膜血管生成效应的刺激效应。结果构建的TPH菌株在体外可有效转染HUVEC并表达有活性的HGF蛋白,6×10~5个HUVEC细胞可表达160~190 ng的HGF蛋白,和对照组相比,TPH转染组细胞表达上清可明显促进HUVEC增殖,而且具有剂量-效应关系;TPH转染组细胞表达上清也刺激鸡胚绒膜尿囊膜小血管生成,其血管数量(133.0±11.5)/cm~2明显大于转染TP的HUVEC细胞上清组(83.3±5.5)/cm~2和对照组(62.7±7.1)/cm~2(P0.05)。结论携带HGF基因的减毒沙门菌可有效转染HUVEC并促进细胞增殖,刺激小血管新生,可能在组织创面愈合中起重要作用,在胃溃疡治疗中有潜在的应用价值。     

Effects of methyl jasmonate and melatonin treatments on the sensory quality and bioactive compounds of harvested broccoli

Harvested broccoli is prone to decline in quality with regard to its appearance and nutrition. In this study, freshly harvested broccoli was treated with methyl jasmonate (MeJA) and melatonin (MT) and stored at 20 °C and the changes in sensory qualities and bioactive compounds were analyzed. The control samples began yellowing on day 2, whereas MeJA and MT treatments delayed the yellowing by 2 and 4 days, respectively. Upon yellowing, sweetness and bitterness of control samples increased sharply, accompanied by the accumulation of bioactive compounds, except for sulforaphane; however, no significant change in volatile components was detected. When the samples started losing their green color, MeJA alleviated the bitterness while increasing the sweetness and sulforaphane content. The bitterness, astringency, umami level, and the content of sulfurous volatiles improved significantly in the MT-treated samples. Moreover, these samples showed high antioxidant activity; the protective effect on V_C and carotenoids was extremely significant.

In the process of yellowing, methyl jasmonate and melatonin treatments effectively improved the color, taste, flavor and bioactive compound content in broccoli.