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71.
进一步研究了抗三尖杉酯碱的HL-60细胞(HR20)抗细胞凋亡的机制及该抗性和抗药性的关系。结果表明,环孢菌素A(CsA)20,10μg·ml-1诱导HL-60细胞发生凋亡,而阻断HR20细胞于G1期,就不能诱导细胞发生凋亡。低浓度的CsA明显增加柔红霉素在HR20细胞内的积聚,其逆转抗药性作用与阻断细胞周期运行无关。CsA10μg·ml-1处理HR20细胞,可引起50kDa的蛋白质高度磷酸化。结果提示:环孢菌素A阻断抗三尖杉酯碱的HL-60细胞于G1期,而诱导敏感的HL-60细胞发生凋亡,其阻断作用与抗药性无关。 相似文献
72.
The quality of low-contrast portal radiographs for radiation therapy can be improved with electronic contrast enhancement. After the image is copied digitally with a laser scanner microdensitometer into 4,096 gray-scale levels (12 bits) and 1,686 X 2,048 pixels, a special software package permits linear, logarithmic, exponential, or sigmoid transformations of the optical density. The precise representation of the portal image can then be interactively adjusted to emphasize the desired anatomy. Clinical examples demonstrate the value of the digital enhancement approach. 相似文献
73.
Spacing of cytochrome oxidase blobs in visual cortex of normal and strabismic monkeys 总被引:3,自引:3,他引:0
Murphy KM; Jones DG; Fenstemaker SB; Pegado VD; Kiorpes L; Movshon JA 《Cerebral cortex (New York, N.Y. : 1991)》1998,8(3):237-244
Some models of visual cortical development are based on the assumption that
the tangential organization of V1 is not determined prior to visual
experience. In these models, correlated binocular activity is a key element
in the formation of visual cortical columns, and when the degree of
interocular correlation is reduced the models predict an increase in column
spacing. To examine this prediction we measured the spacing of columns, as
defined by cytochrome oxidase (CO) blobs, in the visual cortex of monkeys
whose binocular vision was either normal or disrupted by a strabismus. The
spatial distribution of blobs was examined in seven normal and five
strabismic macaques. Tangential sections through the upper layers of the
visual cortex were stained to reveal the two-dimensional (2D) pattern of CO
blobs. Each blob was localized and their center-to-center spacing, packing
arrangement and density were calculated using 2D nearest-neighbor spatial
analyses. The mean center-to-center spacing of blobs (590 microm for
normally reared and 598 microm for strabismic macaques) and the mean
density of blobs (3.67 blobs/mm2 for normally reared and 3.45 blobs/mm2 for
strabismic macaques) were not significantly different. In addition, the 2D
packing arrangement of the blobs was not affected by strabismus. While it
is clear that neural activity plays a key role in the elaboration and
refinement of ocular dominance cortical modules, we conclude that it does
not determine the spatial period of the pattern of CO blobs. This suggests
that aspects of the neural circuitry underlying the columnar architecture
of the visual cortex are established prenatally and its fundamental
periodicity is not modifiable by experience.
相似文献
74.
Pancreas divisum: thin-section CT 总被引:1,自引:0,他引:1
Zeman RK; McVay LV; Silverman PM; Cattau EL; Benjamin SB; Fleischer DF; Garra BS; Jaffe MH 《Radiology》1988,169(2):395-398
Twelve patients with known pancreas divisum underwent thin-section computed tomography (CT) to determine the capability of CT to depict this pancreatic anomaly. Focal pancreatic enlargement was present in five patients. Two distinct pancreatic moieties separated by a fat cleft were noted in three patients; a fourth patient had focal atrophy in the distribution of the dorsal pancreas. The two pancreatic moieties were identified at the same craniocaudal level in all four of these patients. The dorsal duct was depicted in all 12 patients, while the short ventral duct was seen in only five of the 12 patients. Failure of the ventral and dorsal pancreatic ducts to fuse was identified in all five patients in whom both ducts were seen. CT may not enable specific diagnosis of pancreas divisum in the majority of patients. If, however, distinct pancreatic moieties or unfused ductal systems are evident, the diagnosis may be confidently suggested. 相似文献
75.
76.
Graat HC Witlox MA Schagen FH Kaspers GJ Helder MN Bras J Schaap GR Gerritsen WR Wuisman PI van Beusechem VW 《British journal of cancer》2006,94(12):1837-1844
Despite improvements in treatment regimens for osteosarcoma (OS) patients, survival rate has not increased over the last two decades. New treatment modalities are therefore warranted. Preclinical results with conditionally replicative adenoviruses (CRAds) to treat OS are promising. One type of CRAd that was effective against OS cells is Ad5-Delta24RGD. In other types of cancer, CRAds have been shown to interact synergistically with chemotherapeutic agents. Chemotherapy for OS often includes doxorubicin and cisplatin. Therefore, we explored combination treatment of OS cell lines and primary OS cell cultures with Ad5-Delta24RGD and doxorubicin or cisplatin. On OS cell lines, combination treatment was additive to synergistic. Surprisingly, however, on seven of eight primary OS samples no such combination effects were observed. In contrast, in many cases chemotherapy even inhibited CRAd-mediated cell killing. The inhibitory effect of doxorubicin on Ad5-Delta24RGD in primary OS cells appeared to correlate with slow cell growth rate; reduced viral replication and absence of chemotherapy-induced G2 cell cycle arrest. Our results point to the possibility that, at least for OS, virotherapy and chemotherapy should best not be performed simultaneously. In general, our work underscores the importance of testing new genetic anticancer agents and treatment regimens on primary cancer specimens. 相似文献
77.
The pathogenesis of diphenylhydantoin-induced pure red cell aplasia was investigated in the case of a 32-year-old man who developed pure red cell aplasia while he was under treatment with diphenylhydantoin. The patient's serum IgG purified from serum drawn at the time of diagnosis suppressed normal allogeneic marrow colony-forming (CFU-E) and burst- forming (BFU-E) and autologous blood BFU-E growth in vitro only in the presence of diphenylhydantoin. This IgG-diphenylhydantoin complex had no effect on CFU-GM growth in vitro. Normal IgG or patient's IgG purified from serum drawn after the remission of red cell aplasia had no effect on erythroid colony formation in vitro in the presence of diphenylhydantoin. The IgG-diphenylhydantoin complex exerted no direct cytotoxic effect on normal marrow erythroblasts, CFU-E, and BFU-E, nor did it interfere with the action of erythropoietin on marrow erythroblasts. These studies suggest that diphenylhydantoin-induced red cell aplasia is immunologically mediated through an IgG inhibitor, which requires the presence of the drug to suppress erythroid colony formation in vitro. This inhibitor seems to exert its effect on erythroid progenitors at or beyond the stage of differentiation of CFU- E, but not on erythroblasts. 相似文献
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