周围神经端侧缝合与神经移植的实验研究

目的　比较周围神经端侧缝合与神经移植的效果。方法　选用体重 2 0 0～ 30 0gWistar大白鼠 ,左侧后肢腓总神经与胫神经端侧缝合 ,右侧腓总神经采用神经移植修复。结果　 3个月后运动神经传导速度分别为2 9.6 8± 5 .34m/s、 30 .87± 6 .0m/s(P >0 .0 5 ) ,潜伏期 2 .1± 0 .1ms ,2 .0± 0 .1ms(P >0 .0 5 ) ,波幅 12 .5± 0 .6mV、13.9± 0 .5mV(P >0 .0 5 ) ,组织切片中 ,两组均可见大量神经纤维和髓鞘 ,有髓神经纤维计数分别为 75 7.2± 2 2 .31、775± 2 1.87(P >0 .0 5 )。结论　①正常神经发出侧芽能通过端侧缝合口长入远端神经 ,使变性神经再神经化 ;②周围神经端侧缝合能取得与神经移植相近的结果。     

Chronic Effects of Bromate on Sexual Reproduction of Freshwater Rotifer Brachionus calyciflorus

The effects of potassium bromate (KBrO₃), sodium bromate (NaBrO₃), and potassium bromide (KBr) on the sexual reproduction of the rotifer Brachionus calyciflorus were studied by 2-d population growth, 4-d sexual reproduction, and 7-d resting egg production tests. The results showed that low concentrations of bromate promote 2-d and 4-d rotifer population growth, while high concentrations limit it. Bromate stress significantly affected parameters of rotifer sexual reproduction, including the ratio of mictic to amictic females, the mictic rate of rotifers, and the fertilization rate of mictic females. KBrO₃ at 0.001, 0.01, 1, and 10 mg/L, NaBrO₃ at 1 and 10 mg/L, and KBr at 100 and 200 mg/L significantly increased resting egg production, while KBrO₃ at 100 and 200 mg/L, and NaBrO₃ at 200 mg/L significantly decreased it. Resting egg production appears to provide a sensitive endpoint in evaluating the effect of bromate on rotifer sexual reproduction.

     

An Environmental Dilemma for China During the COVID-19 Pandemic: The Explosion of Disposable Plastic Wastes

Plastic pollution control has been on top of the political agenda in China. In January 2020, China announced a phased ban on the production and usage of various types of single-use plastics as a solution to environmental pollution problems. However, the outbreak of COVID-19 seems to be a new obstacle to the ban on single-use plastic products. To basically satisfied the daily necessities and contain the spread of SARS-CoV-2 under the background of the regular epidemic prevention and control in China, online ordering, contactless delivery and wearing mask have become an important and feasible way of daily life. However, the unrestrained use of disposable plastic bags, lunch boxes and masks within the nationwide quarantine leads to hundreds of millions of plastics wastes every day. The potential environmental pollution caused by the use of disposable plastic products during the pandemic should arouse social concern. The Chinese government should manage environmental protection in parallel with anti-pandemic endeavors as the situation of the pandemic evolves.

     

刺五加对肺纤维化BALF中IL－6抑制作用的研究

在用石棉灌肺制成大鼠支气管肺纤维化模型基础上，应用刺五加注射液进行体内实验治疗。测定了治疗及非治疗组大鼠支气管肺泡灌洗液中单个核细胞自分泌白细胞介素６（ＩＬ－６）水平，经统计学处理两组差异显著（Ｐ＜０．０１）。说明了刺五加对ＩＬ－６的分泌有抑制作用。可能对细胞因子网络紊乱有调节作用，为临床应用刺五加治疗肺纤维化提供了实验依据。     

微电极记录技术在立体定向手术治疗帕金森病中的应用

目的：提高立体定向手术的定位精确度。方法：32例帕金森患者均采用CT定位。通过微电极描述和记录苍白球腹后内侧部（Gpi)及丘脑腹外侧核（Vim)的电生理信号。从而pi和Vim核的电生理靶点。结果：电生理靶点坐标与原始CT靶点坐标的更换率为64%。总有效率为90%,无永久性并发症,结论：微电极记录技术的应用提高了手术治疗帕金森病的效果。降低了手术并发症。     

EGb对MPP^＋诱导的PC12细胞凋亡的影响

目的 观察EGb对MPP^ 诱导的PC12细胞凋亡的细胞。方法 MPP^ 诱导PC12细胞凋亡,AO／EB染色,荧光显微镜下观察凋亡细胞。结果 EGb50、100μg/ml在6h、12h、24h可抑制细胞凋亡,而EGb25μg/ml则无效。结论 EGb在6h、12h、24h时呈剂量依赖性降低细胞凋亡率,提示EGb有可能通过抑制DA能神经元凋亡而相对增加DA含量。     

一种新的鼠大脑局部缺血模型

目的 制成鼠大脑局部缺血模型。方法 采用左侧颈动脉离心端插管,灌注一定压力和流量的生理盐水。结果 ①美蓝染色显示大脑左侧大部分区域被染蓝,除皮层着色较浅外,其余部位着色较深。②体感诱发电位在灌注后30min时,其幅度超过灌注前20％。结论 染色说明大脑左侧为缺血区,体感诱发电位幅度的增高,表明大脑处于兴奋性损伤状态。证实了此方法制成的大脑局部缺血可靠,且省时省力简便易行。     

PTCA及血管内支架术中冠脉循环血小板功能的变化

目的 探讨经皮腔内冠脉成形术（PTCA）及冠脉内支架术对冠脉循环中血小板功能的影响。方法 29例冠心病患者分成冠脉造影组（8例）和PTCA加支架组（21例）,分别于术前及术后即刻采集冠状静脉窦血,采用比浊法测定血小板最大聚集率（MPAR）,放免法测定血小板α-颗粒膜蛋白（GMP－140）及血栓素B2（TXB2）的浓度。并对PTCA及支架术中不同时间点血小板功能指标的变化情况进行了观察。结果 冠脉造影前后血小板功能指标无显著变化;PTCA及支架组在球囊预扩张即刻各血小板功能指标均轻度增高;支架后即刻显著升高（P＜0．05）,支架后10min有下降趋势。结论 冠脉造影对冠脉循环血小板功能无显著影响;PTCA及支架术中能使血小板功能激活。     

高三尖杉酯碱对人眼球后成纤维细胞增殖的影响

为探讨高三尖杉酯碱对人眼球后成纤维细胞 (HROF) )增殖的影响 ,采用体外培养人眼球后成纤维细胞 ,用 MTT比色法测定人眼球后成纤维细胞的增殖程度 ,并观察高三尖杉酯碱对 HROFb生长和增殖的影响。结果显示 :MTT比色法能精确反映 HROFb的增殖程度 ,所测光吸收值与细胞数目成正相关 (r=0 .996 ,P<0 .0 0 1 )。高三尖杉酯碱可抑制 HROFb的增殖 ,细胞抑制率与高三尖杉酯碱浓度呈显著正相关 (r=0 .6 932 ,P<0 .0 0 1 )     

舌鳞状细胞癌组织中p53、p21和bcl-2基因蛋白的表达

【目的】研究 p5 3、p2 1和bcl 2基因蛋白在舌鳞状细胞癌组织中的表达及其临床意义。【方法】用免疫组化SP法检测 5 4例舌鳞状细胞癌组织标本p5 3、p2 1和bcl 2癌基因蛋白表达。【结果】舌鳞状细胞癌组织中p5 3、p2 1和bcl 2蛋白阳性率分别为 46 %、6 1%、46 % ;p5 3和 p2 1蛋白表达与肿瘤细胞分化程度及颈淋巴结转移有关 (P <0 0 5 ) ;p5 3蛋白表达与 p2 1蛋白表达有关 (P <0 0 5 ) ,bcl 2基因蛋白表达与 p5 3或p2 1基因蛋白表达无关 (P >0 0 5 )。【结论】舌鳞状细胞癌组织中具有较高的 p5 3、p2 1和bcl 2癌基因蛋白表达 ;p5 3和 /或p2 1蛋白阳性病例恶性程度高 ,易发生颈淋巴结转移。p5 3和 /或 p2 1蛋白可作为舌鳞癌预后的参考指标