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53.
World Health Organization/World Organisation for Animal Health/Food Agriculture Organization HN Evolution Working Group 《Influenza and other respiratory viruses》2014,8(3):384-388
The divergence of the hemagglutinin gene of A/goose/Guangdong/1/1996‐lineage H5N1 viruses during 2011 and 2012 (807 new sequences collected through December 31, 2012) was analyzed by phylogenetic and p‐distance methods to define new clades using the pre‐established nomenclature system. Eight new clade designations were recommended based on division of clade 1·1 (Mekong River Delta), 2·1·3·2 (Indonesia), 2·2·2 (India/Bangladesh), 2·2·1·1 (Egypt/Israel), and 2·3·2·1 (Asia). A simplification to the previously defined criteria, which adds a letter rather than number to the right‐most digit of fifth‐order clades, was proposed to facilitate this and future updates. 相似文献
54.
HELGA LUDWIG-KÖHN H. V. HENNING ASTRID SZIEDAT D. MATTHAEI G. SPITELLER J. REINER H.-J. EGGER 《European journal of clinical investigation》1983,13(1):91-98
Abstract. The neutral steroid fractions in the urine of eleven patients suffering from various forms of liver disease with cholestasis and of ten healthy individuals were studied by glass capillary gas chromatography-mass spectrometry. The steroid conjugates in urine were enzymatically solvolysed, the liberated steroids extracted and transformed into the trimethylsilylether for measurements.
The excretion rates of androstane and pregnane metabolites of patients with liver disease were far lower than those of healthy persons. The main compounds in the urine of the former were the bile alcohols 27 - nor -3α, 7α, 12α, 24, 25 - pentahydroxy - 5β - cholestane and 3α, 7α, 12α, 25, 26 - pentahydroxy - 5β - cholestane. Our data suggest a correlation between the excretion rates of these bile alcohols and the serum levels of bilirubin. While the excretion rate of the two bile alcohols in the urine of healthy individuals was approximately 0.24 mg/24 h (0.6 μmol/24 h) a patient with a serum bilirubin of 841 μmol/1 excreted 4 mg/24 h (9 μmol/24 h). The accumulation of bile alcohols described in this study possibly indicates alternative pathways of cholic acid formation in liver disease. 相似文献
The excretion rates of androstane and pregnane metabolites of patients with liver disease were far lower than those of healthy persons. The main compounds in the urine of the former were the bile alcohols 27 - nor -3α, 7α, 12α, 24, 25 - pentahydroxy - 5β - cholestane and 3α, 7α, 12α, 25, 26 - pentahydroxy - 5β - cholestane. Our data suggest a correlation between the excretion rates of these bile alcohols and the serum levels of bilirubin. While the excretion rate of the two bile alcohols in the urine of healthy individuals was approximately 0.24 mg/24 h (0.6 μmol/24 h) a patient with a serum bilirubin of 841 μmol/1 excreted 4 mg/24 h (9 μmol/24 h). The accumulation of bile alcohols described in this study possibly indicates alternative pathways of cholic acid formation in liver disease. 相似文献
55.
探讨Pre-S2抗原在乙型肝炎病毒的各类隐性感染中的地位及科研、临床应用价值。以乙肝病毒隐性感染者作为研究对象,采用ELISA法,同时检测感染者的Pre-S2抗原和“乙肝血清标志物(HBV M)”,然后进行各类感染者之间的比较、分析。在乙肝病毒隐性感染者人群中,HBeAg阳性者、HBeAb阳性者的Pre-S2抗原阳性率分别为93.10%、60.00%;“乙肝大三阳”、“小三阳”者的Pre-S2抗原阳性率分别为92.31%、62.50%。Pre-S2抗原在判断乙肝病毒复制、隐性感染者血清的传染性强度方面,有很高使用价值和补缺作用;在判断隐性感染者的发展转化方面,亦有重大参考意义。 相似文献
56.
Predominant role of catalase in the disposal of hydrogen peroxide within human erythrocytes 总被引:3,自引:1,他引:3
Purified enzymes were mixed to form a cell-free system that simulated the conditions for removal of hydrogen peroxide within human erythrocytes. Human glutathione peroxidase disposed of hydrogen peroxide (H2O2) at a rate that was only 17% of the rate at which human catalase simultaneously removed hydrogen peroxide. The relative rates observed were in agreement with the relative rates predicted from the kinetic constants of the two enzymes. These results confirm two earlier studies on intact erythrocytes, which refuted the notion that glutathione peroxidase is the primary enzyme for removal of hydrogen peroxide within erythrocytes. The present findings differ from the results with intact cells, however, in showing that glutathione peroxidase accounts for even less than 50% of the removal of hydrogen peroxide. A means is proposed for calculating the relative contribution of glutathione peroxidase and catalase in other cells and species. The present results raise the possibility that the major function of glutathione peroxidase may be the disposal of organic peroxides rather than the removal of hydrogen peroxide. 相似文献
57.
张海宁 《世界华人消化杂志》2006,14(23):2261-2262
0引言《世界华人消化杂志》创刊于1993-01-15,原刊名《新消化病学杂志》,1999-03-25经国家科学技术部和国家新闻出版总署批准更名为《世界华人消化杂志》.《世界华人消化杂志》综合介绍消化病学前沿基础与临床研究的发现,覆盖消化病学领域中经临床实验证明的技术进展.经历了近14 a的发展,本刊在以下几个方面取得了长足的进步. 相似文献
58.
Aaron A Phillips Franco HN Chan Mei Mu Zi Zheng Andrei V Krassioukov Philip N Ainslie 《Journal of cerebral blood flow and metabolism》2016,36(4):647-664
Neurovascular coupling reflects the close temporal and regional linkage between neural activity and cerebral blood flow. Although providing mechanistic insight, our understanding of neurovascular coupling is largely limited to non-physiological ex vivo preparations and non-human models using sedatives/anesthetics with confounding cerebrovascular implications. Herein, with particular focus on humans, we review the present mechanistic understanding of neurovascular coupling and highlight current approaches to assess these responses and the application in health and disease. Moreover, we present new guidelines for standardizing the assessment of neurovascular coupling in humans. To improve the reliability of measurement and related interpretation, the utility of new automated software for neurovascular coupling is demonstrated, which provides the capacity for coalescing repetitive trials and time intervals into single contours and extracting numerous metrics (e.g., conductance and pulsatility, critical closing pressure, etc.) according to patterns of interest (e.g., peak/minimum response, time of response, etc.). This versatile software also permits the normalization of neurovascular coupling metrics to dynamic changes in arterial blood gases, potentially influencing the hyperemic response. It is hoped that these guidelines, combined with the newly developed and openly available software, will help to propel the understanding of neurovascular coupling in humans and also lead to improved clinical management of this critical physiological function. 相似文献
59.
Szekeres T; Fritzer M; Strobl H; Gharehbaghi K; Findenig G; Elford HL; Lhotka C; Schoen HJ; Jayaram HN 《Blood》1994,84(12):4316-4321
Increased ribonucleotide reductase (RR) activity has been linked with malignant transformation and tumor cell growth. Therefore, this enzyme is considered to be an excellent target for cancer chemotherapy. We have examined the effects of a newly patented RR inhibitor, trimidox (3,4,5-trihydroxybenzohydroxamidoxime). Trimidox inhibited the growth of human promyelocytic leukemia HL-60 cells with an IC50 of 35 mumol/L. Incubation of HL-60 cells with 50 mumol/L trimidox for 24 hours decreased deoxyguanosine triphosphate (dGTP) and deoxycytidine triphosphate (dCTP) pools to 24% and 39% of control values, respectively. Incubation of HL-60 cells with 20 to 80 mumol/L trimidox even up to a period of 4 days did not alter the distribution of cells in different phases of cell cycle. Sequential incubation of HL-60 cells with trimidox (25 mumol/L) for 24 hours and then with 10 mumol/L tiazofurin (an inhibitor of inosine monophosphate dehydrogenase) for 4 days produced synergistic growth inhibitory activity, and the cell number decreased to 16% of untreated controls. When differentiation- linked cell surface marker expressions were determined in cells treated with trimidox and tiazofurin, a significantly increased fluorescence intensity was observed for the CD 11b (2.9-fold). CD 33 (1.9-fold), and HLA-D cell surface antigens. Expression of the transferrin receptor (CD71) increased 7.3-fold in cells treated with both agents, compared with untreated controls. Our results suggest that trimidox in combination with tiazofurin might be useful in the treatment of leukemia. 相似文献
60.
Nimisha Gandhi Zainulabedin M. Saiyed Jessica Napuri Thangavel Samikkannu Pichili V. B. Reddy Marisela Agudelo Pradnya Khatavkar Shailendra K. Saxena Madhavan P. N. Nair 《Journal of neurovirology》2010,16(4):294-305
In recent years, increasing interest has emerged to assess the human immunodeficiency virus type 1 (HIV-1) clade C viral pathogenesis
due to its anticipated spread in the United States and other western countries. Previous studies suggest that clade C is less
neuropathogenic than clade B; however, the underlying mechanism is poorly understood. Additionally, the interactive role of
drugs of abuse such as cocaine on clade C-associated neuropathogenesis has not been reported. In the current study, we hypothesize
that HIV-1 clade-specific Tat proteins exert differential effects on blood-brain barrier (BBB) integrity and cocaine further
differentially aggravates the BBB dysfunction. We evaluated the effect of Tat B and Tat C and/or cocaine on the BBB integrity
using an in vitro model constructed with primary human brain microvascular endothelial cells (HBMECs) and astrocytes. The BBB membrane integrity
was measured by transendothelial electrical resistance (TEER) and paracellular permeability was measured by fluorescein isothiocyanate
(FITC)-dextran transport assay and monocytes transmigration across the BBB. Results indicate that Tat B disrupts BBB integrity
to a greater extent compared to Tat C and cocaine further differentially exacerbates the BBB dysfunction. This BBB dysfunction
was associated with altered expression of tight junction proteins zona occuldens (ZO-1) and junctional adhesion molecule (JAM)-2.
Thus, these results for the first time delineate the differential role of Tat B and Tat C and/or cocaine in BBB dysfunction,
which may be correlated with the clade-specific differences observed in HIV-1-associated neurological disorders. 相似文献