全文获取类型
收费全文 | 4374篇 |
免费 | 372篇 |
国内免费 | 47篇 |
专业分类
耳鼻咽喉 | 27篇 |
儿科学 | 98篇 |
妇产科学 | 76篇 |
基础医学 | 532篇 |
口腔科学 | 120篇 |
临床医学 | 390篇 |
内科学 | 1001篇 |
皮肤病学 | 43篇 |
神经病学 | 531篇 |
特种医学 | 532篇 |
外科学 | 417篇 |
综合类 | 56篇 |
一般理论 | 3篇 |
预防医学 | 384篇 |
眼科学 | 23篇 |
药学 | 240篇 |
中国医学 | 6篇 |
肿瘤学 | 314篇 |
出版年
2022年 | 32篇 |
2021年 | 54篇 |
2020年 | 29篇 |
2019年 | 69篇 |
2018年 | 70篇 |
2017年 | 66篇 |
2016年 | 62篇 |
2015年 | 99篇 |
2014年 | 129篇 |
2013年 | 189篇 |
2012年 | 187篇 |
2011年 | 203篇 |
2010年 | 155篇 |
2009年 | 145篇 |
2008年 | 199篇 |
2007年 | 222篇 |
2006年 | 164篇 |
2005年 | 174篇 |
2004年 | 158篇 |
2003年 | 136篇 |
2002年 | 157篇 |
2001年 | 138篇 |
2000年 | 136篇 |
1999年 | 141篇 |
1998年 | 74篇 |
1997年 | 61篇 |
1996年 | 61篇 |
1995年 | 71篇 |
1994年 | 54篇 |
1993年 | 54篇 |
1992年 | 108篇 |
1991年 | 102篇 |
1990年 | 114篇 |
1989年 | 97篇 |
1988年 | 86篇 |
1987年 | 70篇 |
1986年 | 66篇 |
1985年 | 62篇 |
1984年 | 50篇 |
1983年 | 43篇 |
1982年 | 32篇 |
1981年 | 44篇 |
1980年 | 35篇 |
1979年 | 34篇 |
1978年 | 26篇 |
1976年 | 26篇 |
1975年 | 27篇 |
1974年 | 31篇 |
1973年 | 23篇 |
1971年 | 25篇 |
排序方式: 共有4793条查询结果,搜索用时 15 毫秒
41.
J. KRØLL 《Scandinavian journal of immunology》1983,17(S10):171-173
42.
J. KRØLL 《Scandinavian journal of immunology》1983,17(S10):165-169
43.
J. KRØLL 《Scandinavian journal of immunology》1983,17(S10):135-139
44.
45.
46.
The OPAR mouse monoclonal antibody (mAb) directed against rat hepatocytes was previously shown to inhibit adhesion of TA3/Ha mammary carcinoma cells to hepatocytes. The antigen is abundantly present at the surface of hepatocytes beneath the endothelium of liver capillaries where we have observed invasion of carcinoma cells to occur. The OPAR mAb reacted with three major bands on a Western blot of liver plasma membrane proteins. The same proteins were also seen upon immunoprecipitation from iodinated liver plasma membrane proteins. We have isolated OPAR antigens by lectin wheat germ agglutinin (WGA) and OPAR affinity chromatography. Amino acid sequence analysis revealed that two of the bands were 1-macroglobulin and C4-binding protein, which are serum components produced by hepatocytes. The presence of the epitope on distinct proteins and our previous observation that it can be detected in the Golgi apparatus but not in the endoplasmic reticulum, suggested that OPAR reacts with a liver-specific glycoconjugate. Loss of OPAR reactivity after neuraminidase and N-glycosidase F treatment showed that the epitope contains sialic acid residues on N-linked sugar moieties. OPAR also reacted with rat fibronectin, and inhibited adhesion of TA3/St cells to fibronectin. This explains the inhibition by the OPAR mAb of TA3/St cell adhesion to hepatocytes, which we have shown to be due mainly to interaction with hepatocyte surface-associated fibronectin. However, adhesion of the related TA3/Ha cells to hepatocytes, which is mediated by the 6P4 integrin, and does not involve binding to fibronectin, is also inhibited. This suggests that 64 on liver-metastasizing carcinoma cells binds to an OPAR epitope-carrying glycoprotein produced by hepatocytes. 相似文献
47.
Seelen MA Trouw LA van der Hoorn JW Fallaux-van den Houten FC Huizinga TW Daha MR Roos A 《Clinical and experimental immunology》2003,134(2):335-343
In systemic lupus erythematosus (SLE), autoantibodies directed against complement components of the classical pathway, especially against C1q, are associated with severe disease and are of prognostic value for flares of lupus nephritis. Mannose-binding lectin (MBL), the recognition unit of the MBL pathway of complement activation, has structural similarities to C1q. Deficiencies of MBL have been shown to predispose to the development of SLE and to influence the course of the disease. We hypothesized that the presence of autoantibodies to MBL, analogous to autoantibodies to C1q in patients with SLE, may contribute to disease development. The occurrence of anti-MBL autoantibodies was assessed by enzyme-linked immunosorbent assay (ELISA) of 68 serum samples from 20 patients with SLE and in serum from 70 healthy controls. Levels of antibodies directed against MBL were significantly higher in patients with SLE compared to healthy subjects. No significant difference was found between patients with active disease compared to those with inactive disease. While the occurrence of anti-C1q autoantibodies was associated with renal involvement, no such relationship was found for anti-MBL autoantibodies. A significant correlation was found between anti-MBL and anti-C1q antibody levels. The level of anti-MBL antibodies was negatively correlated with MBL-complex activity of circulating MBL. Anti-MBL autoantibodies were of the immunoglobulin G (IgG) isotype and the binding site of IgG anti-MBL was located in the F(ab')2 portion. We conclude that anti-MBL are present in sera from SLE patients and influence the functional activity of MBL. 相似文献
48.
G M de Boo A Tibben J B Lanser A Jennekens-Schinkel J Hermans M Vegter-van der Vlis R A Roos 《Journal of medical genetics》1997,34(7):564-568
Intelligence in 20 presymptomatic subjects with an increased risk (> 95%) for carrying the gene for Huntington's disease (HD) was studied in a prospective, case-control, single blind study. No significant differences between the groups were detected for intelligence indices and subtest scores (Wechsler Adult Intelligence Scale). The high level of the performance IQ and the significant discrepancy between performance IQ and verbal IQ found in both the high risk and the low risk groups contrasted with our expectations based on anamnestic information, general clinical opinion, and the results of previously conducted studies. We propose that psychosocial circumstances could explain the test results and discuss the consequences of our findings for clinical genetics practice. 相似文献
49.
LuSIV cells: a reporter cell line for the detection and quantitation of a single cycle of HIV and SIV replication 总被引:14,自引:0,他引:14
A single cycle of viral replication is the time required for a virus to enter the host cell, replicate its genome, and produce infectious progeny virions. The primate lentiviruses, human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV), require on average 24 h to complete one cycle of replication. We have now developed and characterized a reporter assay system in CEMx174 cells for the quantitative measurement of HIV/SIV infection within a single replication cycle. The SIV(mac)239 LTR (-225 --> +149) was cloned upstream of the firefly luciferase reporter gene and this reporter plasmid is maintained in CEMx174 cells under stable selection. This cell line, designated LuSIV, is highly sensitive to infection by primary and laboratory strains of HIV/SIV, resulting in Tat-mediated expression of luciferase, which correlates with viral infectivity. Furthermore, manipulation of LuSIV cells for the detection of luciferase activity is easy to perform and requires a minimal amount of time as compared to current HIV/SIV detection systems. The LuSIV system is a powerful tool for the analysis of HIV/SIV infection that provides a unique assay system that can detect virus replication prior to 24 h and does not require virus to spread from cell to cell. Thus these cells can be used for the study of replication-deficient viruses and the high throughput screening of antivirals, or other inhibitors of infection. 相似文献
50.
Tumour necrosis factor-alpha stimulates invasiveness of T-cell hybridomas and cytotoxic T-cell clones by a pertussis toxin-insensitive mechanism. 下载免费PDF全文
Tumour necrosis factor-alpha (TNF-alpha) stimulated invasion by mouse T-cell hybridomas and cytotoxic T-lymphocyte clones into rat embryo fibroblast monolayers. The effect on these highly invasive cells was limited: invasion was stimulated maximally to 130% of controls. However, when cells were pretreated with pertussis toxin (PT), which inhibits invasion to +/- 20% of controls, a clearcut effect was observed: 400 U TNF-alpha per ml stimulated invasion usually two- to threefold, and sometimes even up to 10-fold. Therefore, experiments were done with PT-pretreated cells. Stimulation was dose dependent and maximal at 200-400 U TNF-alpha per ml. An anti-TNF-alpha monoclonal antibody completely abolished TNF-alpha-induced invasion. The effect was maximal 30 min after addition of cells and TNF-alpha to the monolayer and then declined. TNF-alpha preincubation of T-cell hybridoma cells, but not of fibroblasts, had a similar stimulatory effect, which was also maximal after 30 min. This shows that TNF-alpha acts directly on the T-cell hybridoma cells. Invasive T-cell hybridomas colonize many tissues from the blood similarly as normal T cells. Our data thus suggest that TNF-alpha can stimulate migration of normal T lymphocytes into inflamed tissues and can promote metastasis of malignant T lymphomas. The signals involved are transmitted via a pertussis toxin-insensitive pathway. 相似文献