Efficient purification of the biosurfactant viscosin from Pseudomonas libanensis strain M9-3 and its physicochemical and biological properties

Viscosin (1), an effective surface-active cyclic lipopeptide, was efficiently recovered from Pseudomonas libanensis M9-3 with a simple purification protocol. A major pigment also obtained during this process was identified as phenazine-1-carboxylic acid. The critical micelle concentration (cmc) of viscosin was determined to be 54 mg L (-1), and the minimum surface tension between air and water at the cmc was 28 mN m (-1). Viscosin forms stable emulsions even at low concentrations (7.5 mg L (-1)), and the conditional stability constant for a cadmium-viscosin complex was determined to be 5.87. The physicochemical properties measured for viscosin are similar to other well-studied biosurfactants such as rhamnolipid and surfactin. Viscosin inhibited migration of the metastatic prostate cancer cell line, PC-3M, without visible toxicity. These properties suggest the potential of viscosin in environmental and biomedical applications.     

Lung cancer in patients with head and neck cancer. Incidence and long-term survival

Of the 3,907 cases of primary head and neck or lung cancer diagnosed between 1961 and 1984, 94 patients were identified with a history of cancer at both sites. The total incidence of lung cancer in our head and neck cancer patients was 5.4 percent. Of the 94 patients, 73 had both cancers diagnosed at our institution. These 73 patients were further analyzed. Squamous cell carcinoma accounted for 63 percent of the lung cancers. Twenty of the lung cancers were synchronous and 47 were metachronous after head and neck cancer. Of the synchronous lung cancers, 50 percent were postoperative stage I, whereas only 11 percent of the metachronous cancers were postoperative stage I. The lung cancer survival rate was significantly better for the synchronous cancer group at 5 years (34 percent) than for the metachronous cancer group (5 percent). The better survival rate was evidently due to the greater proportion of early-stage lung lesions. The relatively large number of advanced-stage lung lesions in the metachronous cancer group suggests that aggressive screening of head and neck cancer patients for lung cancer may detect more metachronous lung cancers at an earlier stage and thus improve the survival rate of these patients.     

Impact of oral rehydration therapy on maternal beliefs and practices related to acute diarrhea

Maternal beliefs and practices related to acute diarrhea were evaluated in 69 villages of a district in Haryana. Oral rehydration therapy (ORT) programme was introduced in 47 villages; in 25 by health workers and in 22 by health volunteers. Twenty two villages served as control. Impact was assessed by interviewing 200 mothers initially and 210 each, one and two year after initiating the programme. Dehydration as a complication of diarrhea was perceived by 67.2% mothers in health worker and 71.4% in volunteer villages of Intervention area as compared to only 32.8% in control area (P < 0.01). In Intervention area, 88.6% mothers had tried ORT as compared to 22.8% in control area and 85% of the mothers found it to be useful. The restriction of breast feeding during acute diarrheal episode was favoured by only 15.7% mothers in intervention area and by 47.1% in control area (P < 0.01). The knowledge regarding use of home available fluids in increased amounts did not show an impressive change. There was no change as regards understanding of cause of diarrhea. Favourable change in beliefs and practices is possible by instituting ORT with culturally relevant health education.     

Stage Ie malignant lymphoma of paranasal sinus —A report of 5 cases and review of literature

Primary lymphoma is the most common nonepithelial malignant tumour arising from the paranasal sinus region. It occurs mostly in middle aged and elderly patients with a nonspecific clinical profile resembling that of commoner epithelial sinonasal malignancies. Modified Rappaport’s and the Working Formulation are the frequently used histopathological classification systems. Radiation therapy is the mainstay of treatment with excellent local control of the disease. Most of the failures occur at distant sites outside the treatment area or in the form of disseminateed disease. Chemotherapy, though used in limited number of patients in some series, seems to have a better role to play in the management of these tumours in future as most of the sinonasal lymphomas are of poorly differentiated variety or of unfavourable histology.     

Tubercular endophthalmitis simulating retinoblastoma

PURPOSE: To report a case of tubercular endophthalmitis simulating retinoblastoma. METHODS: Case report. An 8-year-old female presented with a history of complete loss of vision and a white pupillary reflex in the left eye of 3 month's duration. RESULTS: Retinoblastoma could not be excluded on the basis of clinical examination and relevant investigational studies. In the left eye, a computed tomography (CT) scan demonstrated a large vitreous mass with foci of calcification. Enucleation in the left eye was performed, and histopathological examination revealed a chronic granulomatous endophthalmitis and acid-fast bacilli consistent with tubercular pathology. CONCLUSION: This case illustrates that tubercular endophthalmitis with leukocoria and a vitreous mass containing focal calcification may simulate retinoblastoma.     

Improving access to MCH care facilities within existing health infrastructure

To improve maternal-child services (MCH) services within existing health infrastructures, an operational project was initiated in district Ambala, Haryana, in northern India in February 1985. The main project strategies are training of primary health care (PHC) workers, logistic improvement, and monitoring. This paper reports on the 1st year of project operation and deals with the changes in availability of MCH care facilities. Before and after surveys were undertaken in a sample of 71 and 74 villages, respectively, across 3 blocks of the intervention area (population=370,000). 62 villages from 2 adjacent blocks (population=250,000) were also surveyed at the same time as the after survey; these served as concurrent controls. The information was collected from the village leaders and healthworkers by trained fieldworkers. The villages in intervention area registered a substantial improvement in availability of oral rehydration salts (from 0-67.5%), cotrimoxazole for treatment of acute respiratory infection (from 0-54%), contraceptives (from 34-65%), disposable safe birth kit (from 0-54%), scales for weight (from 24-53%), and health education material (from 0-46%) at the end of the 1st year of project operation. Although the developmental characteristics of the villages included in the after survey and in the concurrent control group did not differ significantly, there was a 2-4 fold increase in MCH facilities in the intervention area. A substantial improvement in MCH care facilities can be achieved in a short period of time even within the constraints of an existing health infrastructure through focused efforts in training and logistics improvement.     

Photographic documentation of spontaneous extrusion of a subconjunctival cysticercus cyst

Spontaneous extrusion of a subconjunctival cysticercus cyst is a rare occurrence. The case reported is of a 20-year-old man who presented with a subconjunctival cysticercus cyst that was suspected to be undergoing spontaneous extrusion. The eye was photographed as the parasite extruded through the conjunctival opening with undulating movements over 3-4 min.     

Immobilization of aminothiols on poly(oxyalkylene phosphates). Formation of poly(oxyethylene phosphates)/cysteamine complexes and their radioprotective efficiency

The necessity to apply near-toxic amounts of radioprotective drugs to achieve adequate protection during radiation treatments represents a major problem in human medicine. One of the promising strategies to suppress the toxicity of these drugs involves their incorporation into biocompatible polymers. In this study cysteamine (Cy) was attached to poly(oxyethylene phosphate), POEP, via an ionic bond. Radioprotection of E. coli B cells by this substance and its acute toxicity on male C57 BL mice were measured. The toxicity of Cy immobilized within the poly(oxyethylene phosphate) was significantly lower in comparison to pure Cy while its radioprotective efficiency remained high at half the maximum tolerable dose. The high radioprotective efficiency of the Cy/POEP complexes was further confirmed on mice at different polymer molecular weight characteristics, drug immobilization degrees, application times, and doses. It was found that POEP with molecular weight 4700 Da and containing 24% repeating units with attached Cy has the highest protection potential combined with a depot effect.