全文获取类型
收费全文 | 1407篇 |
免费 | 79篇 |
国内免费 | 5篇 |
专业分类
耳鼻咽喉 | 17篇 |
儿科学 | 50篇 |
妇产科学 | 18篇 |
基础医学 | 123篇 |
口腔科学 | 42篇 |
临床医学 | 138篇 |
内科学 | 267篇 |
皮肤病学 | 11篇 |
神经病学 | 48篇 |
特种医学 | 334篇 |
外科学 | 136篇 |
综合类 | 29篇 |
预防医学 | 137篇 |
眼科学 | 18篇 |
药学 | 70篇 |
1篇 | |
中国医学 | 8篇 |
肿瘤学 | 44篇 |
出版年
2021年 | 20篇 |
2020年 | 9篇 |
2018年 | 21篇 |
2017年 | 9篇 |
2016年 | 20篇 |
2015年 | 22篇 |
2014年 | 16篇 |
2013年 | 50篇 |
2012年 | 51篇 |
2011年 | 55篇 |
2010年 | 45篇 |
2009年 | 44篇 |
2008年 | 37篇 |
2007年 | 39篇 |
2006年 | 46篇 |
2005年 | 34篇 |
2004年 | 22篇 |
2003年 | 43篇 |
2002年 | 32篇 |
2001年 | 29篇 |
2000年 | 36篇 |
1999年 | 34篇 |
1998年 | 44篇 |
1997年 | 37篇 |
1996年 | 39篇 |
1995年 | 48篇 |
1994年 | 39篇 |
1993年 | 37篇 |
1992年 | 20篇 |
1991年 | 7篇 |
1990年 | 23篇 |
1989年 | 46篇 |
1988年 | 30篇 |
1987年 | 30篇 |
1986年 | 41篇 |
1985年 | 32篇 |
1984年 | 24篇 |
1983年 | 18篇 |
1982年 | 17篇 |
1981年 | 25篇 |
1980年 | 21篇 |
1979年 | 13篇 |
1978年 | 24篇 |
1977年 | 20篇 |
1976年 | 16篇 |
1975年 | 17篇 |
1972年 | 11篇 |
1971年 | 8篇 |
1967年 | 8篇 |
1966年 | 10篇 |
排序方式: 共有1491条查询结果,搜索用时 10 毫秒
31.
32.
33.
Compressed sensing cine imaging with high spatial or high temporal resolution for analysis of left ventricular function
下载免费PDF全文
![点击此处可从《Journal of magnetic resonance imaging : JMRI》网站下载免费的PDF全文](/ch/ext_images/free.gif)
34.
35.
二十世纪初 ,广为普及的现代药物只有一个 :乙酰水杨酸 (阿司匹林 )。二十世纪四十年代 ,人类发现第一种抗菌素、第一种批量生产的抗疟药和第一种抗痨药。五、六十年代 ,很快研制出口服避孕药 ,治疗糖尿病的药 ,及治疗精神疾病、各种感染疾病、心血管疾病和癌症的药物。到七十年代 ,几乎每大类疾病都能找到有效药物 ,尽管这些药物的效果不很理想。但全球约有一半的人口似乎依然生活在十九世纪。他们由于贫穷 ,得不到、买不起现代药物 ;因用法不当而不能正确发挥药物疗效。1 975年 ,世界卫生大会提出“基本药物”和“国家药品政策”的概念 ,… 相似文献
36.
Systemic embolic events are known complications of bacterial endocarditis. Embolization of prosthetic valves has previously been reported in the literature. We report a case of embolization of native aortic valve tissue to the popliteal artery as the presenting event in a patient with subacute bacterial endocarditis. To our knowledge, this rare complication has not been previously reported. 相似文献
37.
The cytoskeleton in Chediak-Higashi syndrome fibroblasts 总被引:2,自引:0,他引:2
The Chediak-Higashi syndrome (CHS) trait is expressed in cultured human skin fibroblasts as an abnormal perinuclear concentration of moderately enlarged lysosomes. The cytoskeleton of CHS fibroblasts appears intact. Microtubules are normal in number and morphology, as assessed by colchicine binding studies, antitubulin immunofluorescence, and electron microscopy. Deformability by shear force is unaltered and microfilaments are abundant. However, CHS lysosomes appear to interact abnormally with the cytoskeleton, since the perinculear aggregation partially disperses after depolymerization of cell microtubules with colchicine. These results suggest that CHS is associated with a defect of either the lysosomal membrane itself or of lysosomal membrane- microtubule interaction. 相似文献
38.
Modulation of the RNA-binding activity of a regulatory protein by iron in vitro: switching between enzymatic and genetic function?
下载免费PDF全文
![点击此处可从《Proceedings of the National Academy of Sciences of the United States of America》网站下载免费的PDF全文](/ch/ext_images/free.gif)
A Constable S Quick N K Gray M W Hentze 《Proceedings of the National Academy of Sciences of the United States of America》1992,89(10):4554-4558
The iron-responsive element-binding protein (IRE-BP) is an RNA-binding protein that regulates the expression of several mRNAs in response to availability of cellular iron. The iron-dependent control of IRE-BP activity has been reconstituted in vitro. Incubation of purified IRE-BP with iron salts in the presence of the reducing agent cysteine decreases IRE-BP binding to the cognate RNA element. The specificity of this effect is established by several parameters: (i) the interaction of the spliceosomal protein U1A with its U1 small nuclear RNA target sequence as an internal control is unaffected by iron perturbations, (ii) non-iron metals fail to mimic the iron effect, and (iii) iron chelator activates the IRE-binding activity of IRE-BP and titrates the effect of iron salts. Modulation of IRE-BP activity by chelatable iron is reversible and thus does not involve permanent alterations of the integrity of the protein. These findings accurately mirror the physiological basis for iron regulation of transferrin receptor mRNA stability as well as ferritin and erythroid 5-aminolevulinate synthase mRNA translation in vivo. We discuss these data vis-a-vis the structural homology of IRE-BP with the iron-sulfur protein aconitase and propose a mechanism by which the same cytoplasmic protein serves a dual function as an RNA-binding factor and an enzyme. 相似文献
39.
The contribution of von Willebrand factor (vWF)-platelet binding to platelet-collagen interaction was examined in vitro. The binding of vWF to platelets was mediated and regulated by ristocetin. Subthreshold concentrations of ristocetin (less than or equal to 1 mg/mL), insufficient to cause ristocetin-induced platelet aggregation (RIPA), were added to platelet-rich plasma (PRP) prior to the addition of collagen. The collagen-induced platelet aggregation (CIPA) was modified by ristocetin and the degree of alteration was dependent on the ristocetin concentration. Response as a function of ristocetin concentration was designated the Collagen-Platelet Aggregation Response (CoI-PAR). In normal PRP the CoI-PAR was a progressive inhibition followed by decreasing inhibition and then an enhanced response. The enhanced response occurred over a narrow range of ristocetin concentrations (0.8 to 1.0 mg/mL). In the absence of vWF (severe von Willebrand's disease, Type I, vWF less than 1%) the CoI-PAR was a progressive, eventually complete inhibition with no enhanced response (with ristocetin concentrations up to 3.0 mg/mL). With addition of vWF to this PRP an enhanced response was observed at a ristocetin concentration inversely proportional to the vWF level. PRP from a patient with severe Hemophilia A showed a response within the normal range. Subthreshold ristocetin did not cause plasma protein precipitation or platelet release of 3H-serotonin, nor induce micro platelet aggregate formation. Digestion of platelet membrane glycoproteins (GP(s] with chymotrypsin demonstrated that upon removal of GPI, RIPA was absent, CIPA retained and the CoI-PAR was progressive inhibition, with no enhancement. With removal of GPs I, II, and III, RIPA, CIPA, and the CoI-PAR were absent. A dose-response 125I-vWF- platelet binding occurred with increasing ristocetin concentrations which was unchanged by the addition of collagen. These results demonstrated that ristocetin-platelet association inhibited CIPA, and vWF-platelet binding enhanced platelet-collagen adhesion and platelet aggregation. The in vitro-enhanced CIPA represents a vWF-dependent aggregation of sufficient magnitude to overcome the inhibitory effect of ristocetin. These studies demonstrate an influential interaction of ristocetin, vWF, and collagen with the platelet membrane and imply an important hemostatic contribution of vWF-platelet binding in platelet- collagen interaction. 相似文献
40.
Fiona Ashford Angus Best Steven J. Dunn Zahra Ahmed Henna Siddiqui Jordan Melville Samuel Wilkinson Jeremy Mirza Nicola Cumley Joanne Stockton Jack Ferguson Lucy Wheatley Elizabeth Ratcliffe Anna Casey Tim Plant The COVID- Genomics UK Consortium Joshua Quick Alex Richter Nicholas Loman Alan McNally 《Journal of clinical microbiology》2022,60(4)