Regulation on the biocompatibility of bioactive titanium metals by type I collagen

To study the regulation on the biocompatibility of titanium metal surface structure, the interaction between the collagen and the titanium surface structure were studied with titanium surfaces subjected to anodic oxidation and acid-alkali treatment. The cell response on the treated surfaces was studied in vitro experiments of MG63 osteoblasts. The effects of different collagen adsorption ability on the biomineralization were investigated with simulated body fluid (SBF) experiment and osteoblasts culture experiments in a mineralization culture medium. It was found that the collagen adsorption ability was controlled by the wettability. The acid-alkali treated titanium could adsorb much more collagen on its surface. The abilities of cell attachment and proliferation were improved after collagen soaking. The apatite formation ability was inhibited in SBF after collagen adsorption on the surfaces, but improved in cell-involved situation. The ALP and OCN activity of MG63 cells assay showed the collagen on the titanium surface could enhance the bioactivity of the cells, which could accelerate the biomineralization process in cell culture experiments. The result indicated that the different adsorption ability of type I collagen could regulate the biocompatibility of titanium metal surface. It is possible to optimize the biocompatibility of the titanium metals by using suitable surface modification method.     

A prediction model of substrates and non-substrates of breast cancer resistance protein (BCRP) developed by GA-CG-SVM method

Breast cancer resistance protein (BCRP) is one of the key multi-drug resistance proteins, which significantly influences the therapeutic effects of many drugs, particularly anti-cancer drugs. Thus, distinguishing between substrates and non-substrates of BCRP is important not only for clinical use but also for drug discovery and development. In this study, a prediction model of the substrates and non-substrates of BCRP was developed using a modified support vector machine (SVM) method, namely GA–CG–SVM. The overall prediction accuracy of the established GA–CG–SVM model is 91.3% for the training set and 85.0% for an independent validation set. For comparison, two other machine learning methods, namely, C4.5 DT and k-NN, were also adopted to build prediction models. The results show that the GA–CG–SVM model is significantly superior to C4.5 DT and k-NN models in terms of the prediction accuracy. To sum up, the prediction model of BCRP substrates and non-substrates generated by the GA–CG–SVM method is sufficiently good and could be used as a screening tool for identifying the substrates and non-substrates of BCRP.     

Urodynamic changes in a female case of obstructive sleep apnea syndrome with enuresis: 7 years’ follow-up

Obstructive sleep apnea syndrome （OSAS） is characterized by repetitive upper airway occlusion resulting in apnea lasting 10 seconds or more. Clinical manifestations include snoring, daytime somnolence, intellectual deficiency, sexual impotence, and nocturnal polyuria. Enuresis associated with OSAS is suggested to be more common in children than in adults） In children, removal of upper airway obstruction by surgical intervention （tonsillectomy and/or adenoidectomy） leads to a significant decrease in or complete cure of enuresis. Although a great deal has been written concerning aduk enuresis,     

腹膜后副神经节瘤19例临床诊治分析

目的 探讨腹膜后副神经节瘤的临床特征及预后因素,提高腹膜后副神经节瘤的诊治水平.方法 回顾性分析本院1999年11月至2009年3月手术治疗腹膜后副神经节瘤19例患者的临床表现、肿瘤功能、术中所见、手术方式、病理和影像学资料,以及术后生存时间.结果 (1)本组19例腹膜后副神经节瘤男女比例为1.375,中位年龄50岁,最常见的临床表现为腹部肿块(9/19,47%),术前CT诊断误诊率高(89%);(2)肿瘤好发于下腔静脉及腹主动脉周(9/19,47%),平均最大直径为8.6 cm(3～23 cm),58%(11/19)肿块包膜完整,42%(8/19)肿块与周围脏器紧贴或紧密粘连,26%(5/19)需作毗邻受累器官切除;(3)本组63%(12/19)肿瘤为功能性,术前表现高血压占67%(8/12),术中血压波动剧烈占33%(4/12);(4)16例18个肿块行免疫组化染色,89%(16/18)Chg-A(+),67%(12/18)S-100(+),增殖细胞核抗原(PCNA)呈不同程度表达(0%～48%),Ki-67与P53均只在恶性病例阳性表达(分别为20%,34%);(5)本组病例随访1～107个月,中位随访40个月,Kaplan-Meier及Log-Rank生存分析显示:全组总体的5年生存率为77%,生存率与肿瘤出现远处转移相关(χ2=6.604,P=0.01),与肿瘤大小(χ2=3.208,P=0.201)、功能状态(χ2=0.121,P=0.728)、肿瘤局部情况(χ2=0.036,P=0.849)相关不显著.结论 早期诊断腹膜后副神经节瘤较为困难;患者生存预后与转移相关,应强调终生随访;术后病理免疫组化检查对判断肿瘤性质和预后非常必要.     

鼻中隔组织瓣修补上颌骨手术后的硬腭缺损

目的 探讨应用鼻中隔组织瓣修补上颌骨切除后硬腭缺损的效果.方法 对26例行上颌骨手术的患者分别应用全鼻中隔组织瓣(16例)和部分鼻中隔组织瓣(10例)进行修复.结果 26例中21例Ⅰ期愈合,恢复了正常吞咽及发音功能.5例出现瘘孔,2例经换药愈合,3例经2次手术缝合后2例痊愈,1例仍遗留小的瘘孔;外侧颊部略凹4例;6例术后安装义齿不适合;无鼻背下陷者.随访时间6～134个月,中位时间34.5个月.Kaplan-Meier生存曲线计算总的3年、5年、10年的生存率分别为46.2％、30.8％和11.5％.结论 应用鼻中隔组织瓣修复方法优点是鼻中隔不易被侵犯,取材方便,抗感染能力强,易于愈合.鼻中隔有软骨作支架,可减少面部的畸形.该修复方法适合于修复切除硬腭或部分切除硬腭的缺损.     

家兔前肢“商阳”“二间”“三间”“合谷”“曲池”穴巨显微解剖结构

目的:研究家兔前肢阳明经"商阳"二间"三间"合谷"曲池"穴区的层次结构。方法:先对各穴进行体表定位标识,然后针刺各穴,用多用电子穴位测定治疗仪协助探穴,以确定上述各穴定位准确性。空气栓塞处死家兔,打开胸腔后从锁骨下动脉注入不同浓度的ABS灌注液,使前肢血管全部染色清晰,在巨显微条件下逐一对腧穴进行层次解剖,观察并测量腧穴与周围组织结构的毗邻关系。结果:家兔前肢阳明经"商阳"二间"三间"合谷"曲池"穴浅层穴区以头静脉为主,桡神经浅支神经干和其分支为基础;深层穴区以桡动脉及分支、正中神经为基础。结论:"商阳"二间"三间"合谷"曲池"穴和头静脉,桡动、静脉及分支,桡神经浅支及正中神经有密切的关系,这是上述5个腧穴穴区的形态学基础。     

Smad4 expression in hepatocellular carcinoma differs by hepatitis status

Aims: Primary hepatocellular carcinoma (HCC) is a common malignancy often related to hepatitis viralinfection. Smad4 is known to mediate the TGF-β pathway to suppress tumorigenesis. However, the function ofSmad4 in HCC is still controversial. In this study we compared levels of Smad4 in HCC tissues with or withouthepatitis virus infection and adjacent normal-appearing liver. Methods: Samples from HCC patients wereanalyzed for Smad4 protein and mRNA expression by immunohistochemistry (IHC), RT-PCR and Westernblotting. Results: We found that tumor tissues expressed less Smad4 mRNA and protein than the adjacent tissues.Most HCC tumor tissues were negative for Smad4 in IHC staining, while the majority of adjacent tissues werepositively stained. Interestingly, protein levels were higher in HCC tissues with viral hepatitis than those withoutvirus infection. Suppression of expression appeared closely related to HCC, so that Smad4 appears to functionas a tumor suppressor gene (TSG). Conclusion: Patients with hepatitis viral infection, at higher risk for HCC,exhibited increased Smad4 protein expression suggesting hepatitis virus may modulate Smad4 expression, whichis functionally distinct from its putative role as a TSG. Smad4 expression may thus be an applicable marker fordiagnosis and/or a target to develop therapeutic agents for HCC.     

陕西省黄陵县农村50～75岁妇女贫血危险因素分析

目的 分析陕西省黄陵县农村老年妇女贫血的相关危险因素.方法 以西北地区抽样点陕西省黄陵县50～75岁妇女为研究对象,进行血红蛋白(Hb)筛查和基本健康状况询问,选择200例病例组人员,按年龄1:1配对选择200名对照组人员.对两组的一般状况、饮食情况进行问卷调查,测定相关血液生化指标.结果 贫血组和对照组的个人年收入分别为(446.1±107.9)、(903.8±179.1)元(t=3.06,P＜0.01),平均每天活动时间分别为(9.6±3.2)、(10.3±3.1)h(t=1.94,P＜0.05)、经历食物短缺比例分别为31.8%、22.6%(χ2=4.14,P＜0.05)、腰围分别为(76.2±7.3)、(79.5±8.9)cm(t=4.08,P＜0.01).贫血组和对照组的总蛋白分别为(78.0±5.8)、(81.9±6.0)g/L(t=5.94,P＜0.01)、血清铁分别为(13.9±5.7)、(16.1±5.0)μmol/L(t=4.19,P＜0.01)、铁蛋白分别为(94.9±76.4)、(116.6±85.2)μg/L(t=2.58,P＜0.01)、转铁蛋白饱和度分别为22.9%±10.0%、25.6%±8.7%(t=3.16,P＜0.01).多因素条件logistic回归分析显示,个人年收入、是否经历食物短缺、平均每天活动时间、主食、豆类及其制品、能量的OR值分别为:0.57、4.74、0.06、0.59、0.55、0.65.95%CI分别为0.45～0.71、0.73～30.56、0.01～0.52、0.38～0.91、0.34～0.87、0.44～0.98.结论 黄陵地区老年贫血妇女的膳食质量、体格状况和相关血液指标均低于对照组,收入水平低、活动时间少、主食、豆类及其制品、能量摄入量低为贫血的危险因素.