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排序方式: 共有438条查询结果,搜索用时 7 毫秒
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Susanne H Hodgson Prateek Choudhary Sean C Elias Kathryn H Milne Thomas W Rampling Sumi Biswas Ian D Poulton Kazutoyo Miura Alexander D Douglas Daniel GW Alanine Joseph J Illingworth Simone C de Cassan Daming Zhu Alfredo Nicosia Carole A Long Sarah Moyle Eleanor Berrie Alison M Lawrie Yimin Wu Ruth D Ellis Adrian V S Hill Simon J Draper 《Molecular therapy》2014,22(12):2142-2154
The development of effective vaccines against difficult disease targets will require the identification of new subunit vaccination strategies that can induce and maintain effective immune responses in humans. Here we report on a phase 1a clinical trial using the AMA1 antigen from the blood-stage Plasmodium falciparum malaria parasite delivered either as recombinant protein formulated with Alhydrogel adjuvant with and without CPG 7909, or using recombinant vectored vaccines—chimpanzee adenovirus ChAd63 and the orthopoxvirus MVA. A variety of promising “mixed-modality” regimens were tested. All volunteers were primed with ChAd63, and then subsequently boosted with MVA and/or protein-in-adjuvant using either an 8- or 16-week prime-boost interval. We report on the safety of these regimens, as well as the T cell, B cell, and serum antibody responses. Notably, IgG antibody responses primed by ChAd63 were comparably boosted by AMA1 protein vaccine, irrespective of whether CPG 7909 was included in the Alhydrogel adjuvant. The ability to improve the potency of a relatively weak aluminium-based adjuvant in humans, by previously priming with an adenoviral vaccine vector encoding the same antigen, thus offers a novel vaccination strategy for difficult or neglected disease targets when access to more potent adjuvants is not possible. 相似文献
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Cesare Hassan Marco Spadaccini Andrea Iannone Roberta Maselli Manol Jovani Viveksandeep Thoguluva Chandrasekar Giulio Antonelli Honggang Yu Miguel Areia Mario Dinis-Ribeiro Pradeep Bhandari Prateek Sharma Douglas K. Rex Thomas Rösch Michael Wallace Alessandro Repici 《Gastrointestinal endoscopy》2021,93(1):77-85.e6
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Comparison of conventional and Hang-back methods of inferior oblique recession in V-pattern strabismus with inferior oblique overaction 下载免费PDF全文
Kamlesh Anan Ankita Bharadwaj Shweta Dhiman Yashpal Goel Anju Rastogi Richa Agarwal Manisha Mishr Prateek Kumar 《国际眼科》2020,13(6):985-990
AIM: To investigate the regulatory roles of the members of the peroxisome proliferator-activated receptor (PPAR) family in lacrimal gland dysfunction under conditions of desiccating stress or diabetes. METHODS: Quantitative polymerase chain reaction (qPCR) was used to examine the expression of PPARs in the cornea, conjunctiva, meibomian gland, and lacrimal gland in adult rats. The rats were divided into 3 groups: a control group, dry eye group, and diabetic group. The phenol red threads test, tear film break-up time (BUT) test and fluorescein staining were carried out to evaluate the development of dry eye. Based on bioinformatics research, qPCR was used to examine the expression level of PPARγ, tumor necrosis factor-α (TNF-α), interleukin-β (IL-β), interleukin-6 (IL-6), sirtuin 1 (Sirt1), myeloid differentiation factor 88 (MyD88) and transforming growth factor-β (TGF-β) in the lacrimal glands.RESULTS: PPARα and PPARβ/δ were mainly expressed in the conjunctiva and the lacrimal gland, respectively. However, PPARγ was expressed in both the conjunctiva and lacrimal gland, at much higher levels than those measured for PPARα and PPARβ/δ. Dry eye rats and diabetic rats both showed decreased tear secretion, shortened BUT, and increased corneal staining. Significant changes in gene expression were observed compared with the control group. In the lacrimal glands of dry eye rats and diabetic rats, expression of PPARγ decreased (P<0.05), expression of Sirt1 also decreased (P<0.01), whereas expression of TNF-α, IL-β, IL-6, MyD88, and TGF-β increased (P<0.05).CONCLUSION: Among PPARs, PPARγ might play a dominant role in the regulation of metabolic- and inflammatory-signaling pathways on the ocular surfaces and in lacrimal glands. Down-regulation of PPARγ is highly relevant to lacrimal gland dysfunction under desiccating-stress and diabetic conditions. PPARγ, thus, is a potential therapeutic target in the treatment of environment- or diabetes-induced dry eye diseases. 相似文献
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Cathryn A Broderick Alexander J Smith Kam S Balaggan Anastasios Georgiadis Anastasios Georgarias Prateek K Buch Peter C Trittibach Susie E Barker Gian-Marco Sarra Adrian J Thrasher Andrew D Dick Robin R Ali 《Molecular therapy》2005,12(2):369-373
Autoimmune posterior uveitis is a chronic, potentially blinding inflammatory disease of the eye. It is commonly treated with immunosuppressive drugs that have adverse long-term effects. Advances in gene transfer techniques have enabled long-term, stable transduction of retinal cells following subretinal injection with adeno-associated viral (AAV) vectors. Here we report for the first time that subretinal injection of rAAV-2 encoding murine IL-10 into the retina of C57BL/6 mice significantly decreases the median experimental autoimmune uveitis (EAU) disease severity. This protection is shown to be due to a decrease in the number and activation status of infiltrating monocytes during EAU, as determined by costimulatory molecule expression and nitrotyrosine detection. No differences within splenocyte proliferative responses or serum antibody levels were detected, emphasizing the potential of gene therapy strategies in ameliorating autoimmune responses in local microenvironments without unwanted systemic effects. 相似文献
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Pinto Benzeeta Deo Prateek Sharma Susmita Syal Arshi Sharma Aman 《Clinical rheumatology》2021,40(10):3883-3896
Clinical Rheumatology - Deficiency of adenosine deaminase 2 (DADA2) is a monogenic disease caused by biallelic mutations in ADA2 gene (previously CECR1). The aim of this review was to... 相似文献