Clinical use and late results of transvenous endocardial destruction of the atrioventricular junction by the bipolar method

Transvenous endocardial destruction of the AV junction, using an original bipolar technique, was carried out in 32 patients with supraventricular tachyarrhythmias, resistant to medication. The procedure was effective in 29 (91%) of 32 patients. Stable and complete AV block was achieved in 23 of 30 patients, with subsequent implantation of a pacemaker, while in 4 patients, clinical improvement was achieved with intact AV conductivity. Two patients with re-entry tachycardia of the AV junction were subjected to low-energy contusion of the AV junction, and the results were good. Major hemodynamic and myocardial contractility parameters were basically unchanged at long-term follow-up; spontaneous heart rate was significantly decreased in patients with implanted pacemakers, and some of those developed pacemaker dependence.     

A method of portalizing the venous blood flow of the left adrenal gland

The authors, having studied the long-term results of the operation of creating the renoportal venous anastomoses in patients with severe and malignant course of arterial hypertension, revealed anastomotic thrombosis in presence of hypotensive effect. Developed in the experiment and employed in 7 patients was the technique of dosaged narrowing the central left adrenal vein with the aim of achieving its thrombosis, inclusion of the collateral routes of the left adrenal blood outflow into the system of the portal vein and inactivation of aldosterone in the liver.     

Treatment of gunshot wounds of soft tissues

The authors have determined principal directions of a complex and successive action on the wound process taking into account its phasic character and new ideas as the dynamics of zonal disturbances of microcirculation around the gunshot wound. The use of the systemic treatment of 44 patients with gunshot wounds of soft tissues allowed the period of treatment to become 1.5 times shorter and the amount of suppurations was 4 times less.     

Effect of the physico-chemical form and storage time of ferriferrocyanides on the sorption of cesium-137 in the digestive tract

In consequence of the test of Ferriferrocyanide efficiency under the intoxication of white rats by 137Cs the dependence of its sorption effect on the chemical form, production process and the degree of dispersion of the preparation is demonstrated. Maximum efficiency has been determined in Ferriferrocyanides when their degree of dispersion is 0.02-0.03 mm. Antidote qualities of the preparations can be preserved for 15 years.     

Effect of different factors modifying the activity of some enzyme systems of the endoplasmic reticulum on the sensitivity of cell organelles against the damaging action of chemical agents. II. Studies with chlorpromazine, 2,4-dinitrophenol, phenobarbital and DDT.

Further investigations on the effect of different stress factors on the stability of intracellular membranes were carried out. Large granule fractions derived from livers of sleep-deprived and dehydrated rats and subjected to preincubation at 37 degrees and pH 5 were shown to release latent acid phosphatase with a delayed rate indicating an increased lysosomal stability towards acid media conditioning. Lysosomes of such animals, however, were found to be more sensitive to mechanical treatments (homogenization procedure in this case) than that of controls, a conclusion made on the basis of enhanced "free" and nonsedimentable phosphatase activities in liver homogenates. The stress factors which previously were included in the group of modifiers of the activity of the endoplasmic reticulum-located enzymes caused some changes in the action of certain chemicals on membranes. Earlier such changes were elicited for carbon tetrachloride and only on low-temperature-conditioned rats for chlorpromazine. The present results show that stress factors studied result in deviations (different in extent and in direction) from the usual effects of chlorpromazine, 2,4-dinitrophenol, phenobarbital and DDT on liver lysosomes and peroxisomes.     

Direct mechanical heart massage

Biomedical Engineering -     

Histoautoradiographic and biochemical study of the transport of atherogenic lipoproteins in the vascular wall]

Transport of serous pre-beta and beta-lipopropeins (beta-LP) labeled by C14 protein and by C14 lipid into the wall of the aorta was studied histoautoradiographically and biochemically. The aortas of healthy rabbits and rabbits wuth experimental atherosclerosis were perfused in vitro with a medium containing rabbit pre-beta and beta-LP during 6 hours. After perfusion the mark was identified histoautoradiographically in all layers of the aorta. The presence of gradient in distribution of the mark from the inner layers of the aortas towards the outward part of the media indicated that the process of infiltration of the wall of the LP vessel was caused by their penetration from the lumen of the aorta. Nonuniformity in distribution of LP penetrated into the atherosclerotic aorta was noted, which was considered in connection with the changes occurred in the connective tissue of the wall of the aorta in the process of atherogenesis. Results of both morphological and biochemical investigations confirm an increased permeability of the aorta wall for LP in atherosclerosis and also substantiate the assumption about penetration of pre-beta and beta-LP into the wall of the vessel as a whole particle.     

Ultrastructure of viable and inactivated Coxiella burnetii after inoculation of mice.

C3HA mice were inoculated intraperitoneally (i.p.) with viable Coxiella burnetii (C.b.) (strain Apodemus flavicollis-Luga, phase I) or C.b. antigen (formalin-inactivated C.b.); pieces of spleen, liver, omentum, kidney, and suspension of peritoneal macrophages were examined by electron microscopy at 1, 7, 14, 21, 28, and 42 days post-infection (p.i.). Two main types of viable C.b. cells--small dense (DC) and bacteria-like (BC)--were found in omentum, spleen, and peritoneal exudate cells beginning from the 1st day p.i. but maximal amount they reached in spleen at day 14. Some parasitophorous vacuoles contained damaged BC or lysing C.b. cells. C.b. corpuscular antigen was found in phagosomes in ultrathin sections of spleen macrophages until 28 days p.i. By 7 days p.i. in omentum 3 types of parasitophorous phagosomes were described which were supposed to be the sites of the antigen utilization. It is assumed that most BC are destroyed in omentum macrophages during the 1st week after i.p. injection of the antigen, and DC can persist in spleen at least for one month.