全文获取类型
收费全文 | 4234篇 |
免费 | 283篇 |
国内免费 | 21篇 |
专业分类
耳鼻咽喉 | 36篇 |
儿科学 | 147篇 |
妇产科学 | 88篇 |
基础医学 | 592篇 |
口腔科学 | 138篇 |
临床医学 | 414篇 |
内科学 | 885篇 |
皮肤病学 | 80篇 |
神经病学 | 560篇 |
特种医学 | 151篇 |
外科学 | 413篇 |
综合类 | 12篇 |
预防医学 | 306篇 |
眼科学 | 100篇 |
药学 | 259篇 |
中国医学 | 2篇 |
肿瘤学 | 355篇 |
出版年
2023年 | 38篇 |
2022年 | 70篇 |
2021年 | 130篇 |
2020年 | 86篇 |
2019年 | 110篇 |
2018年 | 126篇 |
2017年 | 133篇 |
2016年 | 140篇 |
2015年 | 133篇 |
2014年 | 164篇 |
2013年 | 206篇 |
2012年 | 311篇 |
2011年 | 294篇 |
2010年 | 165篇 |
2009年 | 137篇 |
2008年 | 256篇 |
2007年 | 284篇 |
2006年 | 247篇 |
2005年 | 286篇 |
2004年 | 239篇 |
2003年 | 196篇 |
2002年 | 189篇 |
2001年 | 46篇 |
2000年 | 45篇 |
1999年 | 44篇 |
1998年 | 42篇 |
1997年 | 32篇 |
1996年 | 24篇 |
1995年 | 25篇 |
1994年 | 20篇 |
1993年 | 12篇 |
1992年 | 19篇 |
1991年 | 11篇 |
1990年 | 8篇 |
1989年 | 18篇 |
1988年 | 18篇 |
1987年 | 10篇 |
1986年 | 12篇 |
1985年 | 19篇 |
1984年 | 20篇 |
1983年 | 18篇 |
1982年 | 8篇 |
1979年 | 10篇 |
1978年 | 15篇 |
1977年 | 7篇 |
1974年 | 12篇 |
1972年 | 14篇 |
1971年 | 8篇 |
1970年 | 7篇 |
1969年 | 6篇 |
排序方式: 共有4538条查询结果,搜索用时 15 毫秒
61.
Chiaravalloti Nancy D. Amato Maria Pia Brichetto Giampaolo Chataway Jeremy Dalgas Ulrik DeLuca John Meza Cecilia Moore Nancy B. Feys Peter Filippi Massimo Freeman Jennifer Inglese Matilde Motl Rob Rocca Maria Assunta Sandroff Brian M. Salter Amber Cutter Gary Feinstein Anthony 《Journal of neurology》2021,268(5):1598-1607
Journal of Neurology - Individuals with pre-existing chronic illness have shown increased anxiety and depression due to COVID-19. Here, we examine the impact of the COVID-19 pandemic on emotional... 相似文献
62.
Francesco Manfredi Danilo Abbati Beatrice Claudia Cianciotti Lorena Stasi Alessia Potenza Eliana Ruggiero Zulma Magnani Erica Carnevale Matteo Doglio Maddalena Noviello Elena Tassi Chiara Balestrieri Silvia Buonanno Francesca Clemente Claudia De Lalla Maria Pia Protti Anna Mondino Giulia Casorati Paolo Dellabona Chiara Bonini 《European journal of immunology》2021,51(8):1992-2005
The phenotype of infused cells is a major determinant of Adoptive T-cell therapy (ACT) efficacy. Yet, the difficulty in deciphering multiparametric cytometry data limited the fine characterization of cellular products. To allow the analysis of dynamic and complex flow cytometry samples, we developed cytoChain, a novel dataset mining tool and a new analytical workflow. CytoChain was challenged to compare state-of-the-art and innovative culture conditions to generate stem-like memory cells (TSCM) suitable for ACT. Noticeably, the combination of IL-7/15 and superoxides scavenging sustained the emergence of a previously unidentified nonexhausted Fit-TSCM signature, overlooked by manual gating and endowed with superior expansion potential. CytoChain proficiently traced back this population in independent datasets, and in T-cell receptor engineered lymphocytes. CytoChain flexibility and function were then further validated on a published dataset from circulating T cells in COVID-19 patients. Collectively, our results support the use of cytoChain to identify novel, functionally critical immunophenotypes for ACT and patients immunomonitoring. 相似文献
63.
Vasileios Kapralos Håkon Valen Rukke Dag Ørstavik Andreas Koutroulis Josette Camilleri Pia Titterud Sunde 《Dental materials》2021,37(2):249-263
ObjectivesAssess the antibacterial, physical and chemical properties of AH Plus, BioRoot RCS and Pulp Canal Sealer (PCS) in contact with 2% chlorhexidine digluconate (CHX) used as final irrigant prior to root canal obturation.MethodsThe antimicrobial properties were investigated by direct contact tests for planktonic and biofilm growth of E. faecalis, S. mutans, S.epidermidis and S.aureus in vitro. The setting time, wettability, microhardness and surface roughness were also assessed. The sealers were studied in no contact, 1-minute (short-term) and continuous contact (long-term) with CHX. Chemical characterization of sealers was performed by scanning electron microscopy, X-ray diffraction analysis and Fourier-transform infrared spectroscopy after CHX or saline used as the last irrigant in an ex vivo tooth model and in endo training blocks.ResultsCHX increased the antibacterial activity of all the sealers investigated against planktonic bacteria and biofilms with PCS exerting the highest antimicrobial activity with and without the presence of CHX. The setting of AH Plus and BioRoot RCS was retarded, while for PCS accelerated in the presence of CHX. AH Plus and PCS were more hydrophilic after contact with CHX, whilst BioRoot RCS was hydrophobic in a time-dependent manner. The microhardness of sealers was compromised and the surface roughness increased after CHX exposure for AH Plus and BioRoot RCS, and decreased for PCS. CHX did not affect the sealers’ chemistry, but PCS that exhibited two extra phases.SignificanceCHX improved the antibacterial efficacy of endodontic sealers but further evidence is needed to confirm its suitability as a final irrigant prior to root canal obturation. 相似文献
64.
Kristiana Gordon Sarah L. Spiden Fiona C. Connell Glen Brice Sally Cottrell John Short Rohan Taylor Steve Jeffery Peter S. Mortimer Sahar Mansour Pia Ostergaard 《Human mutation》2013,34(1):23-31
Milroy disease (MD) is an autosomal dominantly inherited primary lymphedema. In 1998, the gene locus for MD was mapped to 5q35.3 and variants in the VEGFR3 (FLT4) gene, encoding vascular endothelial growth factor receptor 3 (VEGFR3), were identified as being responsible for the majority of MD cases. Several reports have since been published detailing pathogenic FLT4 mutations. To date, a total of 58 different variants in FLT4, 20 of which are unpublished, have been observed in 95 families with MD. A review of published mutations is presented in this update. Furthermore, the unpublished variants are presented including clinical data. Comparison of clinical features in patients and their families with the same mutations reveals incomplete penetrance and variable expression, making genotype–phenotype correlations difficult. Most mutations are missense, but a few deletions and one splicing variant have also been reported. Several animal models have confirmed the role of VEGFR3 in lymphangiogenesis and studies show mutant VEGFR3 receptors are not phosphorylated. Here, an MD patient with the same p.Ile1053Phe change as seen in the Chy mouse is presented for the first time. This finding confirms that this mouse lineage is an excellent model for MD. All the data reviewed here has been submitted to a database based on the Leiden Open (source) Variation Database (LOVD) and is accessible online at www.lovd.nl/flt4. 相似文献
65.
D. James Harris Maria Pia Spigonardi João P. M. C. Maia Regina T. Cunha 《Acta parasitologica / Witold Stefański Institute of Parasitology, Warszawa, Poland》2013,58(4):607-611
Water frogs, Pelophylax perezi, that are introduced in the Azores, were screened for parasites using PCR primers known to amplify Apicomplexa parasites, and using nematode-specific primers. With the former, three different organisms were detected: Hepatozoon, a trichodinid protozoan ciliate and a possible Stramenopile. Using the latter set of primers, a single unknown spirurid nematode was also detected. Phylogenetic analyses indicate that Hepatozoon detected within amphibian hosts appear to form a clade, although relationships of these parasites do not match the vertebrate intermediate host phylogeny. Regarding the possible Stramenopile, it is unclear whether this organism was actually present on the amphibian or in the water on the surface of the tissue sample. Our findings highlight that many different organisms can be detected with these primers and that they can be used to screen introduced host populations to detect parasites that have been brought with them. 相似文献
66.
67.
68.
69.
Maria Wessman Inka Aho Kristina Thorsteinsson Merete Storgaard Isik S Johansen Suzanne Lunding Gitte Pedersen Anne‐Mette Lebech Pia Kivel Marie Helleberg Terese L Katzenstein Nina Weis 《Journal of the International AIDS Society》2015,18(1)
Introduction
As the human immunodeficiency virus (HIV)-positive population ages, issues concerning sexuality and fertility, among others, are becoming relevant. HIV is still surrounded by stigma and taboos, and there have been few studies conducted in industrialized settings concerning these questions. We therefore wanted to investigate the perception of sexuality and fertility in women living with HIV (WLWH) in an industrialized setting, using a questionnaire.Methods
WLWH were recruited at their regular outpatient clinic visits, at the major Departments of Infectious Diseases in Denmark and Finland, from January 2012 to October 2013. A questionnaire was developed, study participants were informed of the nature of study and, if they agreed to participate and signed a consent form, they filled in the questionnaire. Demographic information on the participants was obtained from patient files (in Finland) or from a national HIV cohort (in Denmark). Statistical analysis was performed using STATA®, version 11.Results
In total, 560 women were included in the study. The median age was 44 years. The majority were of white European origin, with fully suppressed HIV viral load, CD4 cell count >350 µL and mild or no symptoms of their HIV infection. A total of 62% were sexually active, stating condom use as their sole form of contraception. Of the sexually inactive women, one-third were in steady relationships. Eighty percent reported prior pregnancies, of which the majority had one or more children. Most children were born prior to the women''s HIV diagnosis and the mode of conception was predominantly natural. One-quarter of the participating women desired pregnancy, while more than half did not. The remaining quarter either stated that they already had the desired number of children or chose not to answer the question. Fourteen percent stated that their HIV diagnosis ended their wish for children; of these women, the median time of diagnosis was between 1995 and 1996. Pregnancy had been attempted unsuccessfully in one-quarter of study participants. The final question inquired what the risk of mother-to-child transmission was, with all precautions taken. Fifteen percent estimated the risk to be above two percent.Conclusions
In conclusion, the majority of WLWH in industrialized settings in Denmark and Finland have few HIV-related symptoms, are sexually active and have a strong desire for children. 相似文献70.
Ekbom P Damm P Feldt-Rasmussen B Feldt-Rasmussen U Jensen DM Mathiesen ER 《Journal of diabetes and its complications》2008,22(5):297-302
The prevalence of preterm delivery is considerably elevated in women with type 1 diabetes. The aim of the study was to evaluate haemoglobin A(1c) (HbA(1c)) as a predictor of preterm delivery. Two hundred thirteen consecutive pregnant women with type 1 diabetes and normal urinary albumin excretion were included prospectively. HbA(1c) was analyzed at 10, 20 and 28 weeks of gestation. Seventy-one women (33%) delivered pre term and 142 at term. At 10 weeks of gestation, HbA(1c) was 7.3% (S.D. 1.0) vs. 6.9% (S.D. 0.9) (P<.01), at 20 weeks of gestation 6.6% (S.D. 0.7) vs. 6.1% (S.D. 0.7) (P<.001) and at 28 weeks of gestation 6.7% (S.D. 0.8) vs. 6.1% (S.D. 0.7) (P<.001). When comparing HbA(1c) at 10, 20 and 28 weeks of gestation, HbA(1c) at 28 weeks of gestation (P<.001) was the best predictor of preterm delivery. The adjusted odds ratio per 1% increment in HbA(1c) at 28 weeks of gestation was 2.8 (95% CI 1.7-4.4). HbA(1c) at 28 weeks of gestation was a clinical significant predictor of preterm delivery in type 1 diabetes. 相似文献