熏蒸处理日常安全检查

口岸熏蒸处理使用的是有毒有害气体,且高压储存于钢瓶中,要求严格管理,杜绝药品丢失和泄漏。在实际工作当中,接触熏蒸气体的部门和人员,并不能完全规范操作,故日常安全检查对发现安全隐患、规范操作,有着重要的意义。     

2010年西宁地区蝇类监测结果分析

目的掌握西宁地区蝇类密度与季节消长规律,为蝇类防制提供科学依据。方法采用笼诱法。每个监测点设置1个配有新鲜饵料的天幕式捕蝇笼,放置8h(9:00～17:00),收集到的蝇类用乙醚杀死后分类,统计各蝇种数量,并计算蝇类密度。结果西宁市2010年平均蝇类密度为13.06只/笼;优势蝇种为厩腐蝇、新陆原伏蝇;蝇类季节消长呈单峰型,9月份达最高峰。结论孳生环境和防治措施是影响蝇类密度和种群的主要因素。蝇类防制应采取环境治理为主、化学防治为辅的综合防制措施,以达到降低蝇密度、控制传染病发生与流行的目的。     

Co-expression of guanine-nucleotide exchange factor DOCK180 and ELMO1 in ovarian cancer cells

目的 寻找卵巢癌细胞中鸟嘌呤核苷交换因子Dock 180与Elmo1呈相互协同作用的依据.方法 采用Westem blot检测人卵巢癌组织、良性肿瘤组织及正常卵巢组织中的Dock 180与Elmo1的表达水平；免疫组化检测卵巢癌组织中Dock 180与Elmol的分布；免疫荧光检测Dock180与Elmo1在卵巢癌细胞SKOV3中的定位分布；检测Dock180表达缺失的SKOV3细胞中内源性Elmo1的表达水平.结果 Dock 180与Elmo1在人卵巢癌组织中的表达水平呈明显正相关(r=0.829,P＜0.05),在卵巢癌组织中二者的表达水平显著高于卵巢良性肿瘤组织组和卵巢正常组织组(P＜0.05)[Dock180:(1.054±0.114)、(0.518 ±0.126)、(0.425±0.072); Elmo1:(0.864 ±0.114)、(0.374±0.076)、(0.300±0.105)].免疫组化及细胞化学染色均显示二者在人卵巢癌组织和细胞内的分布具有一致性.而且在Dock180表达缺失细胞中,Elmol的表达水平也同时降低.结论 Dock180与Elmo1在促进卵巢癌的癌变过程中可能具有相互协同作用.     

Level set evolution with local uncertainty constraints for unsupervised change detection

This letter presents a novel method for unsupervised change detection (CD) from remote sensing images using level set evolution with local uncertainty constraints (LSELUC). Uncertainty analysis of pixel labels was implemented as prior information to guide the evolution of level curves. Then, local uncertainty and gradient information of level curves were incorporated into the level set energy function to construct local energy constraints. The proposed method can reduce noise, while preserving details in change regions. Furthermore, an advanced regularization strategy of the level set function was adopted to improve the computational efficiency. The performance of the proposed method was validated on two remote sensing data sets. Experimental results show that the proposed method can produce satisfactory CD results.     

Genetic variants in NAMPT predict bladder cancer risk and prognosis in individuals from southwest Chinese Han group

Nicotinamide phosphoribosyltransferase (Nampt) was served as a useful biomarker for tumorigenesis and for the prediction of cancer survival. In the present study, we analyzed the SNPs of the NAMPT gene and their impact on the susceptibility and prognosis for patients with bladder cancer (BC). The rs61330082, rs2505568 and rs9034 were selected and genotyped by polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) method in 407 patients with bladder cancer and 316 ethnicity-matched healthy control subjects. The genotyping method was confirmed by the DNA sequencing analysis. Statistically significant increased bladder cancer risk was found to be associated with the C allele and CC genotype of rs61330082; nevertheless, decreased bladder cancer risk was revealed to be associated with A allele and AT genotype of rs2505568. Stratified analyses revealed the rs61330082 to be statistically associated with increased bladder cancer risk in smokers and increased invasiveness of bladder cancer. The AT heterozygote of rs2505568 may prevent the recurrence of bladder cancer. Kaplan-Meier curves revealed a statistically significant association of rs2505568 with recurrence-free survival for total bladder cancer patients and non-muscle-invasive bladder cancer patients, and a statistically significant association of rs9034 with recurrence-free survival for muscle-invasive bladder cancer patients. Multiple Cox regression analysis identified the rs2505568 as a possible independent prognostic factor for recurrence-free survival in total bladder cancer patients. Our results suggested an important role for NAMPT in the pathogenesis of bladder cancer and SNPs of NAMPT gene might be a novel genetic biomarker for the prognosis of bladder cancer.     

Matteucens I-J,phenolics from the rhizomes of Matteuccia orientalis

Two new phenolics, named matteucens I-J (1-2), were isolated from the 60% EtOH extract of the rhizomes of Matteuccia orientalis (HOOK.) TREV. Their structures were elucidated by means of extensive spectroscopic analysis (HRESIMS, NMR).