Micropropagation, antinociceptive and antioxidant activities of extracts of Verbena litoralis Kunth (Verbenaceae)

This work describes an efficient micropropagation protocol for Verbena litoralis and the study of the antinociceptive and antioxidant activities in extracts of this species. For the establishment in vitro, surface-sterilization procedures and PVPP showed high efficiency in fungal-bacterial contamination and phenol oxidation controls. Nodal segments cultivation in MS medium supplemented with 6-benzyladenine (7.5 μM)/α-naphthaleneacetic acid (NAA; 0.005 μM) induced multiple shoots. Elongated shoots were rooted with IAA (0.2 μM). Acclimatization rates were elevated and the plants showed the typical features of this species. The hexanic fraction (HF) of powdered leaves presented a radical scavenging activity with IC(50) = 169.3 μg mL(-1). HF showed a non-dose dependent analgesic activity in the writhing test; its antinociceptive activity in the hot plate test was restricted to 500 mg kg(-1), which is the highest dose. The results of this study showed the potential of tissue culture on conservation and large scale multiplication and confirmed the traditional folk medicine use of V. litoralis.     

Actigraphic assessment of sleep in chronic obstructive pulmonary disease

Purpose

Previously, sleep in chronic obstructive pulmonary disease (COPD) has been objectively investigated only by lab-based polysomnography. The main purpose of this study was to evaluate sleep quality in COPD patients in their home environment using actigraphy. We also investigated the factors associated with sleep impairment and the relationship between objective and subjective sleep quality and daytime somnolence in these patients.

Methods

Twenty-six patients with moderate to very severe COPD and 15 controls were studied by actigraphy for at least 5 days. Subjective sleep quality was evaluated by the Pittsburgh Sleep Quality Index and daytime sleepiness by the Epworth Sleepiness Scale (ESS). Dyspnea was quantified by the modified Medical Research Council (MMRC) scale.

Results

COPD patients showed increased sleep latency (p?=?0.003), mean activity (p?=?0.003), and wake after sleep onset (p?=?0.003) and reduced total sleep time (TST; p?=?0.024) and sleep efficiency (p?=?0.001), as compared to controls. In patients, severity of dyspnea was correlated with sleep activity (r?=?0.41; p?=?0.04) and TST (r?=??0.46; p?=?0.02) and multiple regression analysis showed that MMRC score was the best predictor of TST (p?=?0.02) and sleep efficiency (p?=?0.03). Actigraphy measures during daytime were not significantly different between patients and controls. Subjective sleep quality was poorer in patients than controls (p?=?0.043). ESS scores were not significantly different between the two groups. Actigraphy measures were not correlated with subjective sleep quality or daytime somnolence in both groups.

Conclusions

Nocturnal sleep is markedly impaired in stable COPD patients studied by actigraphy in their home environment and this impairment is related to severity of dyspnea.     

Evaluation of scleral incisions and their effects on corneal curvature in manual small-incision cataract surgery

Purpose:Incisions in cataract surgery can be modified in various ways in terms of size, shape, and axis to reduce or tailor astigmatism. This study was conducted to examine the effect of site (superior vs, temporal) and shape (frown vs. V-shaped, chevron) of scleral incisions for cataract surgery on corneal curvature.Methods:The prospective study was carried out on 200 consecutive patients with senile cataract and who were planned for surgery at a tertiary eye hospital in north India. The placement of the incision was decided by the steeper corneal meridian—whether superior or temporal—and then patients of these two groups were randomized for frown and V-shaped incision; in this way, four groups of 50 patients each were formed. Follow-up was done on day 1, at 2 weeks, 4 weeks, 8 weeks, and 12 weeks. At each follow-up, post-operative keratometry with routine postoperative examination was done. The results were statistically analyzed by using student’s t-test, Chi-squared test, and the Pearson correlation coefficient.Results:In all the four groups, the difference of preoperative astigmatism and surgically-induced astigmatism was statistically highly significant. The analysis of uncorrected visual acuity (UCVA) was statistically significant (P < 0.05) on postoperative day 1 and at 2, 4, and 12 postoperative weeks; it was statistically insignificant (P > 0.05) at postoperative week 8.Conclusion:Temporal incisions result in lesser postoperative surgically induced astigmatism (SIA) than superior incisions. Chevron incisions result in minimal change in corneal curvature. This effect can be utilized to tailor the postoperative astigmatism.     

A coordinated approach for the assessment of molecular subgroups in pediatric ependymomas using low-cost methods

Journal of Molecular Medicine - Although ependymoma (EPN) molecular subgroups have been well established by integrated high-throughput platforms, low- and middle-income countries still need...     

Light transmission through porcelain.

OBJECTIVE: This study evaluates the effect of shade and thickness of porcelain in light transmission. METHODS: One hundred and twenty-eight disks of Duceram porcelain were made to combine four different thicknesses (1.5; 2.0; 3.0; 4.0 mm) and eight shades (A(1); A(4); B(1); B(4); C(1); C(4); D(2); D(4)). A digital power meter (Newport Optical Power Meter was used to measure light transmission. The porcelain transmission coefficient was calculated using Lambert-Beer law, t(c)=Ce(-alphad), where t(c) is the transmission coefficient, C the contribution factor of the reflection coefficient, e a constant, alpha the absorption coefficient and d is the sample thickness. RESULTS: The transmission coefficients did not vary statistically in relation to the two visible light-curing units studied. From all the samples, the colors A(1) and D(2), thickness 1.5 mm, presented the highest percentages of transmission (8%) and the shades, A(4), B(4) and C(4), thickness 4 mm, the lowest (0.5%). The relationship between the Naperian logarithm of the transmission coefficient and the samples thickness followed the Lambert-Beer law. The linear adjustment of the experimental points of the two variables, showed the absorption coefficient (alpha) and the constant value related to the reflection (C) of each porcelain shade. The reflection coefficient values of all shades did not vary statistically among themselves. SIGNIFICANCE: For most shades there was a significant decrease in light transmission as the sample porcelain thickness increased. For the same thickness most shades presented statistical difference between the transmission coefficients. However, the larger the thickness, the higher the number of shades which, statistically, showed no difference.