An adenosine triphosphate bioluminescence assay for detecting the number of living cells]

The method for detecting the number of living cells was studied. Using an adenosine triphosphate (ATP) bioluminescence assay, the present authors reported a perfect linear relationship between lg ATP concentrations and lg luminescence counts (r = 0.9963) as well as a relationship between lg number of cells and lg ATP luminescence counts (r = 0.9922). The detectable cells ranged from 10(2) to 10(6) cells/ml, the coefficients of variation 1-3%. This method is simple, accurate and sensitive and has a high reproducibility.     

肝素对脂多糖诱导人肾小球系膜细胞增殖及细胞分泌物影响

为了解肝素对脂多糖诱导人肾小球系膜细胞增殖和细胞因子分泌的影响。作者采用四甲基偶氮唑蓝比色分析法及生物活性法,分别测定了人肾小球ＭＣ增殖和细胞因子的分泌情况。结果显示;在正常培养条件下,人肾上球ＭＣ分泌一定理的白细胞介素６（ＩＬ－６０和肿瘤坏死因子α（ＴＮＦ－α）。脂多糖诱导ＭＣ增殖及分泌ＩＬ－６,ＴＮＦ－α。高浓度的肝素（５００Ｕ／ｍｌ）促进ＭＣ增殖及分泌细胞因子,低浓度的肝素（５Ｕ／ｍｌ）抑制     

扑热息痛所致狗急性肝功能衰竭时血浆5-羟色胺变化及肝脏血流动力学改变的实验研究

目的;通过扑热息痛所致急性肝功能衰竭的动物模型,以期探讨门静脉,肝静脉,腹主动脉,下腔静脉血浆５－羟色胺浓度变化和肝脏血流动力学改变。方法：成年杂种狗１２只,在戊巴比妥钠静脉麻醉下,作上腹正中切口,从门静脉,肝静脉,腹主动脉,下腔静脉各采血２ｍｌ,作血浆５－羟色胺含量检测,作楔形肝组织活检,关腹。     

Golytely肠道清洁法应用292例分析

本院自 1 993年初以来 ,用Ｇｏｌｙｔｅｌｙ液对2 92例结、直肠手术及纤维结肠镜检查病人作术前准备均取得较清单效果 ,现介绍如下。1　临床资料1 .1　 一般资料　本组患者共 2 92例 ,其中男性 1 85例 ,女性 1 0 7例。结、直肠手术 1 2 7例 ,诊断结肠肿瘤 72例 ,直肠肿瘤 5 5例。纤维结肠镜检查 1 6 5例。1 .2　配方及使用方法　Ｇｏｌｙｔｅｌｙ液配方〔1〕:聚乙二醇 40 0 0 5 9.1 ｇ,硫酸钠 5 .6 ｇ,氯化钠0 .75 ｇ,碳酸氢钠 1 .3ｇ加水至 1 0 0 0ｍｌ。使用方法 :术前 1ｄ下午服Ｇｏｌｙｔｅｌｙ ,饮速大于1 5 0 0ｍｌ/ｈ ,至排出清液止 …     

哮喘中IL—5和IL—10基因表达及其相关性研究

３１只健康豚鼠分为哮喘组、地塞米松干预组及对照组。测定各组豚鼠肺泡灌洗液细胞成分,应用ＲＴ－ＰＣＲ方法检测支气管组织白细胞介素－５（ＩＬ－５）和白细胞介素－１０（ＩＬ－１０）ｍＲＮＡ表达强度。结果显示,哮喘组豚鼠肺泡灌反。ＩＬ－５ｍＲＮＡ表达与ＥＯＳ百分比呈正相关,而与ＩＬ－１０ｍＲＮＡ表达呈负相关。说明ＥＯＳ,ＩＬ－以正相关参与了哮喘发病。哮喘时ＩＬ－１０表达受抑制,这可能是炎症细胞因子ＩＬ－５     

脑血管病危险因素在海口城乡对比的调查

目的 通过脑血管病危险因素在海口城乡对比的调查,了解脑血管病发病的相关因素。方法 以整群随机抽样法,从超过４０岁人群中取城市１９２人,农村２３１人进行测定血糖、血甭胆固醇、苷油三酯、高密度脂蛋白、低密度脂蛋白、极低密度脂蛋白。结果 经统计学处理,发现胆固醇、苷油三酯高、低密度脂蛋白、极低密度脂蛋白、极低密度脂蛋白城乡比较均有显著差异（Ｐ〈０．０１）,脑血管病发病率、死亡率,城市明显高于乡村（Ｐ〈０     

急性缺血性心肌损伤患者肌钙蛋白T检测的临床意义

目的 :为了早期、准确地判定急性缺血性心肌损伤程度。方法 :采用酶联免疫吸附方法 ,对 41例急性心肌梗塞患者、 38例心绞痛 (AP)疑有微小心肌损伤 (MMD)患者以及 40例健康 (HP)人进行心脏肌钙蛋白 T(c Tn T)测定 ,同时与 CK和CK— MB两项酶学指标相比较。结果 :AMI组 ,c Tn T、CK及 CK— MB三者之间的异常率无明显差别 (异常率 97%～ 10 0 % ) ;而AP组 ,c Tn T的异常率为 44 .7% ,明显高于 CK异常率的 7.9%和 CK— MB异常率的 13.2 %。与健康组相比 ,AP组的 c Tn T测定的水平明显高于对照组水平 (P<0 .0 1)。结论 :对于不同程度的急性缺血性心肌损伤的诊断 ,c Tn T较 CK及 CK— MB,具有更高的灵敏度及特异性。     

房水引流植入物手术治疗顽固性青光眼

目的 :探讨房水机械引流手术在顽固性青光眼治疗中的应用价值。方法 :采用国产房水引流植入物 ,共治疗 11例(11眼 )顽固性青光眼 ,其中新生血管性青光眼 6眼 ,原发性绝对期青光眼 3眼 ,滤过性手术失败者 2眼。结果 :术后随访 3个月～9个月 ,平均 6 .5个月 ,眼压由术前 38mm Hg～ 6 7m m Hg降至术后 7mm Hg～ 33m m Hg。术后常见并发症浅前房 ,前房积血等。结论 :房水引流植入物手术为顽固性青光眼的治疗提供了新的途径。     

慢性重型肝炎124例临床分析

目的 通过１２４例慢重肝临床分析对上前慢重肝治疗疗效、临床难点作一简要分析。方法 １２４例慢重肝进行病原分型、临床分期、分组治疗及疗效判定。结果 ①早、中期慢重父瘟菩Ы暇捌谡呦灾圆钜欤ǎ小矗埃埃担＂谠纭⒅衅诼馗尾∷缆式贤砥谡哂邢灾差异（Ｐ〈０．０５）。③分组治疗病死率无显著性差异（Ｐ〉０．０５）。④死亡组与存活组病例ＴＢ、ＡＬＢ、ＰＴＡ有显著性差异（Ｐ〈０．０５）。结论 ①早、中期慢重肝     

THE OPERATIVE TREATMENT OF SPINAL FRACTURE-DISLOCATION WITHOUT NEUROLOGIC DEFICITS

Objective. To evaluate the results of operative treatment of spinal fracture-dislocation without neumlogic deficits. Metods. Eighteen patients with spinal fracture-dislocation were neurologically intact at the time of injury, and all were treated operatively. The fracture sites were:8 cases in cervical spine, 3 cases in thoracic spine, and 7 cases in lumbar spine. Eight patients with cervical injuries had variant degrees of forward slide and kyphotic deformity. Of the 10 thoracic and lumbar fractures, one had lateral dislocation, 4 cases with kyphotic deformities, 5 cases withspinal canal compromise averaged 50% (ranging from 40% to 70% ).Results. The aveiage period of follow-up was 4.4 years with a range of 11 months to 13 years. All the patients returned to full-time work. No patient developed neurologic deterioration. Kyphotic deformity was corrected in the 4 cases, and no progressive kyphosis was noted. There was no operation-related complication. The averaged post-opera-five hospitalization time was 13 days. Conclusions. Despite the rare incidence of spinal fracture-dislocation without neurologic deficits, we suggested that kind of fracture be considered unstable fracture because of its potential risk of delayed neurologic deterioration and kyphotic deformity, and be treated operatively to restore the sagittal alignment and the stability of the spine.