Inflammatory epidermolysis bullosa acquisita with coexistent IgA antibodies to plectin

We present a case of inflammatory epidermolysis bullosa acquisita (EBA) with IgA antibodies to plectin. Analysis of lesional skin biopsies by electron microscopy revealed the split level to be in the sublamina densa zone, corresponding to the diagnosis of EBA. Direct immunofluorescence of perilesional skin demonstrated u-serrated depositions of IgG and IgA that under immunoelectron microscopy were shown to be located in the sublamina densa. In contrast, indirect immunofluorescence on salt-split skin revealed circulating IgA antibodies that stained the roof rather than the floor of the blister. Immunoblotting showed these serum antibodies to be directed to the cytoplasmic hemidesmosomal antigen plectin. The antiplectin specificity of these antibodies was confirmed by 'knockout' immunofluorescence analysis; the serum IgA did not bind to skin sections of a patient with plectin-deficient epidermolysis bullosa. To our knowledge, this case demonstrates for the first time the existence of IgA antibodies against plectin.     

Population genetic structure and cladistic analysis of Trypanosoma brucei isolates.

Using a novel multilocus DNA marker analysis method, we studied the population genetic structure of Trypansoma brucei stocks and derived clones isolated from animal and rhodesiense sleeping sickness patients during a national sleeping sickness control program in Mukono district, Uganda. We then performed a cladistic analysis to trace relationships and evolution, using stocks and clones recovered from geographically and temporally matched hosts, including inter-strain comparisons with T. b. gambiense stocks and clones. Our results show that while there was close genetic relatedness among parasite populations from the same geographical region, micro-heterogeneities exist between different stocks. Data are presented that indicate that not every human sleeping sickness focus may be associated with a particular human-infective trypanosome strain responsible for long-term stability of the reference focus. We provide evidence of genetic sub-structuring among type 1 T. b. gambiense stocks, which has potentially important implications for molecular epidemiology of T. brucei.     

Evaluating asymmetry in prosthetic gait with step-length asymmetry alone is flawed

Prosthetic gait is often asymmetric in step length, but the direction of this asymmetry varies inconsistently across amputees. This situation is akin to that seen in stroke patients, where step-length asymmetry has been shown to be the additive result of asymmetries in trunk progression and asymmetries in forward foot placement relative to the trunk. The present study examined the validity of this notion in three trans-tibial and seven trans-femoral amputees wearing a unilateral prosthesis while walking over a walkway at a comfortable and slower-than-comfortable speed. The latter manipulation was added to examine the expectation that the magnitude of the trunk-progression asymmetry - attributable to a weaker propulsion generating capacity on the prosthetic side - would be smaller when walking slower because of the diminished propulsion demands. Step length, forward foot placement relative to the trunk, and trunk progression of prosthetic and non-prosthetic steps, as well as asymmetries therein, were quantified. The direction of step-length and forward foot placement asymmetries varied inconsistently across (but consistently within) participants. As expected, step-length asymmetry depended on the combination of asymmetries in forward foot placement and trunk progression, with a smaller contribution of trunk-progression asymmetry at slow speed. These results extend our previous finding for hemiplegic patients that an analysis of gait asymmetry in terms of step length alone is flawed to prosthetic gait, implying that knowledge of asymmetries in trunk progression and forward foot placement relative to the trunk is required to help elucidate the contribution of underlying impairments (viz. propulsion generating capacity) and adopted compensations on prosthetic gait asymmetry.