甲基强的松龙冲击治疗难治性肾病综合征疗效分析

目的探讨甲基强的松龙冲击疗法对难治性肾病综合征的疗效。方法选择38例难治性肾病综合征患儿,给予甲基强的松龙每次20－30mg／kg,加入10％葡萄糖溶液100ml中静滴、1／d,连用3d,第4－7d口服强的松2mg／(kg·d)。未完全缓解者重复第二疗程。结果冲击治疗后,血浆白蛋白有不同程度的升高,24h尿蛋白定量、血胆固醇、血肌酐(Cr)、尿素氮(BUN)有明显降低,冲击治疗前后相差有高度显著性(P<0．01)。随着MP冲击疗程增加,完全及部分缓解例数增多。结论甲基强的松龙冲击疗法可以使难治性肾病达到较稳定的缓解及保护肾功能,尤其对早期病例效果较好,但必须通过前瞻性对照研究加以证实。     

低分子肝素治疗老年人不稳定型心绞痛的疗效分析

目的 观察低分子肝素治疗老年人不稳定型心绞痛的疗效。方法 将１０１例不稳定型心绞痛患者随机分为Ａ、Ｂ两组。在常规治疗基础上Ａ组给予低分子肝素腹部皮下注射;Ｂ组给予等量生理盐水皮下注射。结果 Ａ组心绞痛发作频率比Ｂ组明显减少（Ｐ〈０．０１）。心电图ＳＴ段及Ｔ波较对照组明显改善。Ａ组总有效率９０％,Ｂ组总有效率５４％（Ｐ〈０．０１）。Ａ、Ｂ组治疗期间均未见出血等副作用。结论 低分子肝素治疗老年人不稳定     

The third inactivated vaccine booster dramatically enhanced SARS-CoV-2 antibody responses and did not influence the profile of prothrombotic antibody

The purpose of this study is to investigate the production of both severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2)-specific antibodies and autoantibodies in serum following the third booster vaccination of the inactivated COVID-19 vaccine, and to study the effect of B cell subsets with CD27 and CD38 phenotypes in peripheral blood on antibody production. Routine blood indexes, SARS-CoV-2 antibodies, platelet factor 4 and seven antiphospholipid antibodies were detected both before and 2 months after vaccination in the medical staff of the Zhongnan Hospital of Wuhan University. Peripheral blood B cell subtypes were detected before vaccination. Following immunization, the positive rate of anti-N-S1 immunoglobulin (IgG) had increased from 24.8% to 91.3% and the average antibody concentration had increased by 11 times. The positive rate of neutralizing antibody had increased from 24.8% to 91.3%, the average antibody concentration had increased by 12 times, and the primary increased anti-S1 IgG subtype was that of IgG1. Peripheral blood CD27 + CD38+ B cells were positively correlated with antibody levels after vaccination and were a predictor of the antibody response. In addition, although some indicators showed slight absolute changes, the blood parameters and antiphospholipid antibodies of most volunteers were normal both before and after COVID-19 inactivated vaccine inoculation, and there was no statistical difference in abnormal rates either before or after inoculation. Antibodies in vivo were increased after vaccination with the inactivated vaccine, and IgG1 was the main subtype involved in response to the vaccine. Vaccination with the inactivated COVID-19 vaccine did not appear to affect thrombus-related autoantibodies.     

心理因素在神经性皮炎发病中的作用

用艾森克个性量表对１２５例神经性皮炎患者和１３４例健康志愿者进行心理测试。结果显示,神经性皮炎患者有明显的内倾性－ＥＰＱ－Ｅ分值明显低于对照组（ｔ＝４．５９５,Ｐ〈０．０１）,并且情绪稳定性较低－ＥＰＱ－Ｎ分值明显高于对照组（ｔ＝６．２４５,Ｐ〈０．０１）。结论：社会心理因素在神经性皮炎发病中起重要作用。     

白血病患者血清甲状腺激素水平测定的研究

为了解白血病患者血清甲状腺激素水平的变化,采用放免分析方法测定６７例不同类型白血病患者血清甲状腺激素水平,结果表明急性非淋、慢粒急变及急淋患者,血清三碘甲状腺原氨酸（Ｔ３）水平较正常显著降低,Ｐ〈０．０５,四碘甲状腺原氨酸（Ｔ４）水平无明显变化;促甲状腺激素（ＴＳＨ）仅慢粒急变时较正常升高（Ｐ〈０．０５）,游离Ｔ４（ＦＴ４）急非淋及慢粒急亦患者较正常降低（Ｐ〈０．０５）,而反三碘甲状腺原氨酸（ｒＴ３）较正常升高（Ｐ〈０．０１）,急淋ＦＴ４及ｒＴ３较正常升高（Ｐ〉０．０５）。提示甲状腺激素水平的变化主要在急非淋及慢粒急变者,在急淋变化不突出。     

抗前列腺特异抗原单链抗体/人羧肽酶A融合基因的构建及表达

目的 构建抗前列腺特异抗原（PSA）单链抗体（scFv)/人羧肽酶A(hCPA)融合基因,为前列腺癌的抗全导向酶-前体药物疗法奠定基础。方法 在已克隆抗PSAscFv和hCPA基因的基础上,用加端PCR分别在scFv基因的3′端和hCPA基因的5′端加上部分连接肽（linker)基因序列,回收后混合。应用重叠延伸拼接法,将抗PASscFv基因和hCPA基因通过linker序列串联为scFv/hCPA融合基因;并将构建的融合基因克隆到融合表达载体pGEX-4T-1中进行表达。结果 序列测定结果表明,构建的抗PSAscFv/hCPA融合基因中scFv和hCPA序列均正确,scFv和hCPA间有18bp的linker序列,推导的氨基酸序列为GSGGSG,与设计的序列相符,融合基因经IPTG诱导表达重组蛋白并以SDS-PAGE分析,在Mr为94000左右出现一条新生蛋白带,表达量约占菌体总蛋白的34%。结论 成功构建并原核表达了抗PSAscFv/hCPA融合基因。     

复方环孢菌素A含漱液治疗口腔扁平苔藓的研究

用复方环孢菌素Ａ（ＣｙＡ）含漱液治疗口腔扁平苔藓（ＯＬＰ）３０例,并用口泰治疗作对照。结果发现,复方ＣｙＡ含漱液治疗ＯＬＰ的显效率和总有效率分别为６３％和９７％,显著高于口泰组的１０％和６３％,两者比较差异有显著性（Ｐ〈０．０１）。复方ＣｙＡ无毒副使用,是一种安全、方便、有效地治疗ＯＬＰ的外用药物。     

龙岩市蚊类调查报告

本文报道了1980—2000年闽西地区蚊类调查情况,调查表明;我市蚊为9属36种,未发现微小按蚊及嗜人按蚊,蚊虫全年均有活动,活动高峰为5—10月。人房蚊种主要为中华按蚊、致倦库蚊及三带喙库蚊,夜间活动高峰为21:00—凌晨3:00。同时也调查了白纹伊蚊季节消长及活动规律,为我市控制及消灭丝虫病、疟疾、乙脑及登革热提供了依据。     

不同类型儿童弱视的视觉眼电图研究

目的：探讨不同类型弱视在视网膜水平的改变。方法：对４种类型的弱视儿童８６例分别进行ＶＥＯＧ测试,并与正常对照组进行比较分析。结果：形觉剥夺性弱视组的光峰电位、电势差值和Ａｒｄｅｎ比明显偏高;屈光参差性弱视组光峰电位偏低,光峰时间延长;屈光不正性弱视组中,近视屈光状态者暗谷电位增大,Ａｒｄｅｎ比与Ｇｌｉｅｍ比偏低,远视屈光状态者则无明显变化;斜视性弱视组各参数未见明显差异。结论：提示在视网膜水平不同